Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1913 and Pf1N1B4_1914 overlap by 4 nucleotides Pf1N1B4_1914 and Pf1N1B4_1915 are separated by 22 nucleotides
Pf1N1B4_1913 - Methionyl-tRNA formyltransferase (EC 2.1.2.9), at 2,187,304 to 2,188,263
_1913
Pf1N1B4_1914 - Ribosomal RNA small subunit methyltransferase B (EC 2.1.1.-), at 2,188,260 to 2,189,570
_1914
Pf1N1B4_1915 - Trk system potassium uptake protein TrkA, at 2,189,593 to 2,190,966
_1915
Position (kb)
2188
2189
2190 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2187.287 kb on + strand at 2187.287 kb on + strand at 2187.287 kb on + strand at 2187.287 kb on + strand at 2187.287 kb on + strand at 2187.287 kb on + strand at 2188.503 kb on + strand, within Pf1N1B4_1914 at 2188.504 kb on - strand, within Pf1N1B4_1914 at 2188.515 kb on + strand, within Pf1N1B4_1914 at 2188.515 kb on + strand, within Pf1N1B4_1914 at 2188.515 kb on + strand, within Pf1N1B4_1914 at 2188.516 kb on - strand, within Pf1N1B4_1914 at 2188.812 kb on + strand, within Pf1N1B4_1914 at 2188.813 kb on - strand, within Pf1N1B4_1914 at 2188.813 kb on - strand, within Pf1N1B4_1914 at 2188.813 kb on - strand, within Pf1N1B4_1914 at 2188.813 kb on - strand, within Pf1N1B4_1914 at 2188.813 kb on - strand, within Pf1N1B4_1914 at 2188.813 kb on - strand, within Pf1N1B4_1914 at 2188.860 kb on + strand, within Pf1N1B4_1914 at 2188.860 kb on + strand, within Pf1N1B4_1914 at 2188.861 kb on - strand, within Pf1N1B4_1914 at 2188.877 kb on + strand, within Pf1N1B4_1914 at 2188.877 kb on + strand, within Pf1N1B4_1914 at 2189.330 kb on + strand, within Pf1N1B4_1914 at 2189.331 kb on - strand, within Pf1N1B4_1914 at 2189.331 kb on - strand, within Pf1N1B4_1914 at 2189.331 kb on - strand, within Pf1N1B4_1914 at 2189.331 kb on - strand, within Pf1N1B4_1914 at 2189.331 kb on - strand, within Pf1N1B4_1914 at 2189.368 kb on - strand, within Pf1N1B4_1914 at 2189.497 kb on + strand at 2189.497 kb on + strand at 2189.529 kb on + strand at 2189.530 kb on - strand at 2189.532 kb on + strand at 2189.578 kb on + strand at 2189.579 kb on - strand at 2189.903 kb on - strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2190.023 kb on + strand, within Pf1N1B4_1915 at 2190.023 kb on + strand, within Pf1N1B4_1915 at 2190.023 kb on + strand, within Pf1N1B4_1915 at 2190.023 kb on + strand, within Pf1N1B4_1915 at 2190.023 kb on + strand, within Pf1N1B4_1915 at 2190.024 kb on - strand, within Pf1N1B4_1915 at 2190.070 kb on + strand, within Pf1N1B4_1915 at 2190.070 kb on + strand, within Pf1N1B4_1915 at 2190.071 kb on - strand, within Pf1N1B4_1915 at 2190.071 kb on - strand, within Pf1N1B4_1915 at 2190.071 kb on - strand, within Pf1N1B4_1915 at 2190.071 kb on - strand, within Pf1N1B4_1915 at 2190.071 kb on - strand, within Pf1N1B4_1915 at 2190.074 kb on - strand, within Pf1N1B4_1915 at 2190.215 kb on + strand, within Pf1N1B4_1915 at 2190.215 kb on + strand, within Pf1N1B4_1915 at 2190.215 kb on + strand, within Pf1N1B4_1915 at 2190.240 kb on + strand, within Pf1N1B4_1915 at 2190.283 kb on + strand, within Pf1N1B4_1915 at 2190.283 kb on + strand, within Pf1N1B4_1915 at 2190.283 kb on + strand, within Pf1N1B4_1915 at 2190.283 kb on + strand, within Pf1N1B4_1915 at 2190.283 kb on + strand, within Pf1N1B4_1915 at 2190.283 kb on + strand, within Pf1N1B4_1915 at 2190.283 kb on + strand, within Pf1N1B4_1915 at 2190.283 kb on + strand, within Pf1N1B4_1915 at 2190.283 kb on + strand, within Pf1N1B4_1915 at 2190.283 kb on + strand at 2190.284 kb on - strand, within Pf1N1B4_1915 at 2190.284 kb on - strand, within Pf1N1B4_1915 at 2190.284 kb on - strand, within Pf1N1B4_1915 at 2190.284 kb on - strand, within Pf1N1B4_1915 at 2190.284 kb on - strand, within Pf1N1B4_1915 at 2190.284 kb on - strand, within Pf1N1B4_1915 at 2190.284 kb on - strand, within Pf1N1B4_1915 at 2190.284 kb on - strand, within Pf1N1B4_1915 at 2190.284 kb on - strand, within Pf1N1B4_1915 at 2190.284 kb on - strand, within Pf1N1B4_1915 at 2190.291 kb on + strand, within Pf1N1B4_1915 at 2190.291 kb on + strand, within Pf1N1B4_1915 at 2190.291 kb on + strand, within Pf1N1B4_1915 at 2190.291 kb on + strand, within Pf1N1B4_1915 at 2190.291 kb on + strand, within Pf1N1B4_1915 at 2190.292 kb on - strand, within Pf1N1B4_1915 at 2190.292 kb on - strand, within Pf1N1B4_1915 at 2190.292 kb on - strand, within Pf1N1B4_1915 at 2190.292 kb on - strand, within Pf1N1B4_1915 at 2190.292 kb on - strand, within Pf1N1B4_1915 at 2190.360 kb on - strand, within Pf1N1B4_1915
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 2,187,287 + -1.3 2,187,287 + +1.3 2,187,287 + -2.0 2,187,287 + -0.4 2,187,287 + -1.3 2,187,287 + -0.7 2,188,503 + Pf1N1B4_1914 0.19 -1.4 2,188,504 - Pf1N1B4_1914 0.19 -1.5 2,188,515 + Pf1N1B4_1914 0.19 +0.5 2,188,515 + Pf1N1B4_1914 0.19 +0.3 2,188,515 + Pf1N1B4_1914 0.19 +0.3 2,188,516 - Pf1N1B4_1914 0.20 +1.3 2,188,812 + Pf1N1B4_1914 0.42 -1.5 2,188,813 - Pf1N1B4_1914 0.42 -1.3 2,188,813 - Pf1N1B4_1914 0.42 -0.8 2,188,813 - Pf1N1B4_1914 0.42 +0.3 2,188,813 - Pf1N1B4_1914 0.42 -1.7 2,188,813 - Pf1N1B4_1914 0.42 -2.3 2,188,813 - Pf1N1B4_1914 0.42 -1.0 2,188,860 + Pf1N1B4_1914 0.46 -1.0 2,188,860 + Pf1N1B4_1914 0.46 -2.0 2,188,861 - Pf1N1B4_1914 0.46 -1.3 2,188,877 + Pf1N1B4_1914 0.47 +0.3 2,188,877 + Pf1N1B4_1914 0.47 -0.7 2,189,330 + Pf1N1B4_1914 0.82 -0.7 2,189,331 - Pf1N1B4_1914 0.82 -2.3 2,189,331 - Pf1N1B4_1914 0.82 -1.3 2,189,331 - Pf1N1B4_1914 0.82 -0.7 2,189,331 - Pf1N1B4_1914 0.82 +0.3 2,189,331 - Pf1N1B4_1914 0.82 -1.0 2,189,368 - Pf1N1B4_1914 0.85 -0.0 2,189,497 + -1.1 2,189,497 + -0.3 2,189,529 + -0.1 2,189,530 - +0.3 2,189,532 + -0.7 2,189,578 + +0.3 2,189,579 - -0.6 2,189,903 - Pf1N1B4_1915 0.23 -1.3 2,189,983 + Pf1N1B4_1915 0.28 -0.7 2,189,983 + Pf1N1B4_1915 0.28 +0.3 2,189,983 + Pf1N1B4_1915 0.28 -2.7 2,189,983 + Pf1N1B4_1915 0.28 -0.7 2,189,983 + Pf1N1B4_1915 0.28 -0.7 2,189,983 + Pf1N1B4_1915 0.28 -2.0 2,189,983 + Pf1N1B4_1915 0.28 -0.9 2,189,983 + Pf1N1B4_1915 0.28 -1.7 2,189,983 + Pf1N1B4_1915 0.28 +0.5 2,189,983 + Pf1N1B4_1915 0.28 -0.9 2,189,983 + Pf1N1B4_1915 0.28 -1.3 2,189,983 + Pf1N1B4_1915 0.28 +0.3 2,189,983 + Pf1N1B4_1915 0.28 +0.7 2,189,984 - Pf1N1B4_1915 0.28 -0.1 2,189,984 - Pf1N1B4_1915 0.28 -1.7 2,189,984 - Pf1N1B4_1915 0.28 -0.0 2,189,984 - Pf1N1B4_1915 0.28 -0.3 2,189,984 - Pf1N1B4_1915 0.28 -0.9 2,189,984 - Pf1N1B4_1915 0.28 -0.0 2,189,984 - Pf1N1B4_1915 0.28 -0.7 2,189,984 - Pf1N1B4_1915 0.28 +0.9 2,189,984 - Pf1N1B4_1915 0.28 +0.3 2,189,984 - Pf1N1B4_1915 0.28 -0.7 2,189,984 - Pf1N1B4_1915 0.28 +0.1 2,189,984 - Pf1N1B4_1915 0.28 +0.3 2,189,984 - Pf1N1B4_1915 0.28 -0.7 2,189,984 - Pf1N1B4_1915 0.28 +0.1 2,189,984 - Pf1N1B4_1915 0.28 -0.7 2,189,984 - Pf1N1B4_1915 0.28 -0.3 2,190,023 + Pf1N1B4_1915 0.31 -0.7 2,190,023 + Pf1N1B4_1915 0.31 -2.3 2,190,023 + Pf1N1B4_1915 0.31 -0.0 2,190,023 + Pf1N1B4_1915 0.31 -0.0 2,190,023 + Pf1N1B4_1915 0.31 -0.7 2,190,024 - Pf1N1B4_1915 0.31 +0.8 2,190,070 + Pf1N1B4_1915 0.35 -1.5 2,190,070 + Pf1N1B4_1915 0.35 +1.9 2,190,071 - Pf1N1B4_1915 0.35 +0.6 2,190,071 - Pf1N1B4_1915 0.35 +2.3 2,190,071 - Pf1N1B4_1915 0.35 -0.3 2,190,071 - Pf1N1B4_1915 0.35 -1.5 2,190,071 - Pf1N1B4_1915 0.35 -0.3 2,190,074 - Pf1N1B4_1915 0.35 +0.9 2,190,215 + Pf1N1B4_1915 0.45 -0.2 2,190,215 + Pf1N1B4_1915 0.45 +0.3 2,190,215 + Pf1N1B4_1915 0.45 -0.7 2,190,240 + Pf1N1B4_1915 0.47 +0.3 2,190,283 + Pf1N1B4_1915 0.50 -0.3 2,190,283 + Pf1N1B4_1915 0.50 -0.9 2,190,283 + Pf1N1B4_1915 0.50 -1.7 2,190,283 + Pf1N1B4_1915 0.50 +1.3 2,190,283 + Pf1N1B4_1915 0.50 -1.7 2,190,283 + Pf1N1B4_1915 0.50 -0.7 2,190,283 + Pf1N1B4_1915 0.50 -0.7 2,190,283 + Pf1N1B4_1915 0.50 -1.9 2,190,283 + Pf1N1B4_1915 0.50 +0.3 2,190,283 + -0.7 2,190,284 - Pf1N1B4_1915 0.50 +0.3 2,190,284 - Pf1N1B4_1915 0.50 +1.1 2,190,284 - Pf1N1B4_1915 0.50 +0.3 2,190,284 - Pf1N1B4_1915 0.50 +0.6 2,190,284 - Pf1N1B4_1915 0.50 -0.7 2,190,284 - Pf1N1B4_1915 0.50 -0.3 2,190,284 - Pf1N1B4_1915 0.50 -1.3 2,190,284 - Pf1N1B4_1915 0.50 -2.0 2,190,284 - Pf1N1B4_1915 0.50 +0.3 2,190,284 - Pf1N1B4_1915 0.50 -0.9 2,190,291 + Pf1N1B4_1915 0.51 -1.1 2,190,291 + Pf1N1B4_1915 0.51 +0.7 2,190,291 + Pf1N1B4_1915 0.51 -0.4 2,190,291 + Pf1N1B4_1915 0.51 +0.3 2,190,291 + Pf1N1B4_1915 0.51 +0.3 2,190,292 - Pf1N1B4_1915 0.51 -2.5 2,190,292 - Pf1N1B4_1915 0.51 -0.7 2,190,292 - Pf1N1B4_1915 0.51 -0.2 2,190,292 - Pf1N1B4_1915 0.51 -1.3 2,190,292 - Pf1N1B4_1915 0.51 +0.1 2,190,360 - Pf1N1B4_1915 0.56 +0.9
Or see this region's nucleotide sequence