Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1761 and Pf1N1B4_1762 are separated by 50 nucleotides Pf1N1B4_1762 and Pf1N1B4_1763 are separated by 142 nucleotides
Pf1N1B4_1761 - Fibronectin type III domain protein, at 2,014,076 to 2,015,176
_1761
Pf1N1B4_1762 - FIG00957387: hypothetical protein, at 2,015,227 to 2,016,078
_1762
Pf1N1B4_1763 - putative membrane protein, at 2,016,221 to 2,017,786
_1763
Position (kb)
2015
2016
2017 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2014.446 kb on + strand, within Pf1N1B4_1761 at 2014.446 kb on + strand, within Pf1N1B4_1761 at 2014.620 kb on + strand, within Pf1N1B4_1761 at 2014.620 kb on + strand, within Pf1N1B4_1761 at 2014.620 kb on + strand, within Pf1N1B4_1761 at 2014.621 kb on - strand, within Pf1N1B4_1761 at 2014.629 kb on + strand, within Pf1N1B4_1761 at 2014.629 kb on + strand, within Pf1N1B4_1761 at 2014.716 kb on + strand, within Pf1N1B4_1761 at 2014.716 kb on + strand, within Pf1N1B4_1761 at 2014.716 kb on + strand, within Pf1N1B4_1761 at 2014.716 kb on + strand, within Pf1N1B4_1761 at 2014.716 kb on + strand, within Pf1N1B4_1761 at 2014.716 kb on + strand at 2014.717 kb on - strand, within Pf1N1B4_1761 at 2014.717 kb on - strand, within Pf1N1B4_1761 at 2014.717 kb on - strand, within Pf1N1B4_1761 at 2014.717 kb on - strand, within Pf1N1B4_1761 at 2014.717 kb on - strand, within Pf1N1B4_1761 at 2014.717 kb on - strand, within Pf1N1B4_1761 at 2014.717 kb on - strand, within Pf1N1B4_1761 at 2014.797 kb on + strand, within Pf1N1B4_1761 at 2014.954 kb on + strand, within Pf1N1B4_1761 at 2014.954 kb on + strand, within Pf1N1B4_1761 at 2014.955 kb on - strand, within Pf1N1B4_1761 at 2014.955 kb on - strand, within Pf1N1B4_1761 at 2014.955 kb on - strand, within Pf1N1B4_1761 at 2014.955 kb on - strand, within Pf1N1B4_1761 at 2015.253 kb on + strand at 2015.254 kb on - strand at 2015.310 kb on - strand at 2015.376 kb on + strand, within Pf1N1B4_1762 at 2015.376 kb on + strand, within Pf1N1B4_1762 at 2015.376 kb on + strand, within Pf1N1B4_1762 at 2015.424 kb on - strand, within Pf1N1B4_1762 at 2015.558 kb on + strand, within Pf1N1B4_1762 at 2015.559 kb on - strand, within Pf1N1B4_1762 at 2015.559 kb on - strand, within Pf1N1B4_1762 at 2015.559 kb on - strand, within Pf1N1B4_1762 at 2015.629 kb on - strand, within Pf1N1B4_1762 at 2015.630 kb on + strand, within Pf1N1B4_1762 at 2015.630 kb on + strand, within Pf1N1B4_1762 at 2015.631 kb on - strand, within Pf1N1B4_1762 at 2015.631 kb on - strand, within Pf1N1B4_1762 at 2015.631 kb on - strand, within Pf1N1B4_1762 at 2015.631 kb on - strand, within Pf1N1B4_1762 at 2015.631 kb on - strand, within Pf1N1B4_1762 at 2016.045 kb on + strand at 2016.045 kb on + strand at 2016.078 kb on + strand at 2016.078 kb on + strand at 2016.078 kb on + strand at 2016.078 kb on + strand at 2016.078 kb on + strand at 2016.078 kb on + strand at 2016.078 kb on + strand at 2016.078 kb on + strand at 2016.078 kb on + strand at 2016.078 kb on + strand at 2016.079 kb on - strand at 2016.079 kb on - strand at 2016.079 kb on - strand at 2016.079 kb on - strand at 2016.079 kb on - strand at 2016.090 kb on + strand at 2016.090 kb on + strand at 2016.091 kb on - strand at 2016.091 kb on - strand at 2016.101 kb on + strand at 2016.101 kb on + strand at 2016.102 kb on - strand at 2016.102 kb on - strand at 2016.139 kb on - strand at 2016.152 kb on + strand at 2016.177 kb on + strand at 2016.177 kb on + strand at 2016.178 kb on - strand at 2016.178 kb on - strand at 2016.178 kb on - strand at 2016.178 kb on - strand at 2016.204 kb on - strand at 2016.223 kb on - strand at 2016.234 kb on + strand at 2016.234 kb on + strand at 2016.234 kb on + strand at 2016.234 kb on + strand at 2016.234 kb on + strand at 2016.234 kb on + strand at 2016.234 kb on + strand at 2016.234 kb on + strand at 2016.234 kb on + strand at 2016.234 kb on + strand at 2016.234 kb on + strand at 2016.234 kb on + strand at 2016.234 kb on + strand at 2016.234 kb on + strand at 2016.235 kb on - strand at 2016.235 kb on - strand at 2016.235 kb on - strand at 2016.235 kb on - strand at 2016.235 kb on - strand at 2016.235 kb on - strand at 2016.381 kb on + strand, within Pf1N1B4_1763 at 2016.381 kb on + strand, within Pf1N1B4_1763 at 2016.381 kb on + strand, within Pf1N1B4_1763 at 2016.382 kb on - strand, within Pf1N1B4_1763 at 2016.382 kb on - strand, within Pf1N1B4_1763 at 2016.415 kb on - strand, within Pf1N1B4_1763 at 2016.459 kb on + strand, within Pf1N1B4_1763 at 2016.459 kb on + strand, within Pf1N1B4_1763 at 2016.460 kb on - strand, within Pf1N1B4_1763 at 2016.460 kb on - strand, within Pf1N1B4_1763 at 2016.489 kb on + strand, within Pf1N1B4_1763 at 2016.489 kb on + strand, within Pf1N1B4_1763 at 2016.489 kb on + strand, within Pf1N1B4_1763 at 2016.489 kb on + strand, within Pf1N1B4_1763 at 2016.489 kb on + strand at 2016.489 kb on + strand, within Pf1N1B4_1763 at 2016.489 kb on + strand, within Pf1N1B4_1763 at 2016.489 kb on + strand, within Pf1N1B4_1763 at 2016.489 kb on + strand, within Pf1N1B4_1763 at 2016.489 kb on + strand, within Pf1N1B4_1763 at 2016.490 kb on - strand, within Pf1N1B4_1763 at 2016.490 kb on - strand, within Pf1N1B4_1763 at 2016.490 kb on - strand, within Pf1N1B4_1763 at 2016.490 kb on - strand, within Pf1N1B4_1763 at 2016.490 kb on - strand, within Pf1N1B4_1763 at 2016.490 kb on - strand, within Pf1N1B4_1763 at 2016.490 kb on - strand, within Pf1N1B4_1763 at 2016.490 kb on - strand, within Pf1N1B4_1763 at 2016.492 kb on + strand, within Pf1N1B4_1763 at 2016.492 kb on + strand, within Pf1N1B4_1763 at 2016.492 kb on + strand, within Pf1N1B4_1763 at 2016.492 kb on + strand, within Pf1N1B4_1763 at 2016.493 kb on - strand, within Pf1N1B4_1763 at 2016.493 kb on - strand, within Pf1N1B4_1763 at 2016.493 kb on - strand, within Pf1N1B4_1763 at 2016.493 kb on - strand, within Pf1N1B4_1763 at 2016.498 kb on + strand, within Pf1N1B4_1763 at 2016.498 kb on + strand, within Pf1N1B4_1763 at 2016.498 kb on + strand, within Pf1N1B4_1763 at 2016.498 kb on + strand, within Pf1N1B4_1763 at 2016.498 kb on + strand, within Pf1N1B4_1763 at 2016.498 kb on + strand, within Pf1N1B4_1763 at 2016.498 kb on + strand, within Pf1N1B4_1763 at 2016.498 kb on + strand, within Pf1N1B4_1763 at 2016.498 kb on + strand at 2016.498 kb on + strand, within Pf1N1B4_1763 at 2016.498 kb on + strand, within Pf1N1B4_1763 at 2016.498 kb on + strand, within Pf1N1B4_1763 at 2016.498 kb on + strand, within Pf1N1B4_1763 at 2016.499 kb on - strand, within Pf1N1B4_1763 at 2016.499 kb on - strand, within Pf1N1B4_1763 at 2016.499 kb on - strand, within Pf1N1B4_1763 at 2016.499 kb on - strand, within Pf1N1B4_1763 at 2016.499 kb on - strand, within Pf1N1B4_1763 at 2016.499 kb on - strand, within Pf1N1B4_1763 at 2016.499 kb on - strand, within Pf1N1B4_1763 at 2016.499 kb on - strand, within Pf1N1B4_1763 at 2016.499 kb on - strand, within Pf1N1B4_1763 at 2016.499 kb on - strand, within Pf1N1B4_1763 at 2016.499 kb on - strand at 2016.564 kb on + strand, within Pf1N1B4_1763 at 2016.564 kb on + strand, within Pf1N1B4_1763 at 2016.564 kb on + strand, within Pf1N1B4_1763 at 2016.564 kb on + strand, within Pf1N1B4_1763 at 2016.564 kb on + strand, within Pf1N1B4_1763 at 2016.564 kb on + strand, within Pf1N1B4_1763 at 2016.564 kb on + strand, within Pf1N1B4_1763 at 2016.564 kb on + strand, within Pf1N1B4_1763 at 2016.565 kb on - strand, within Pf1N1B4_1763 at 2016.565 kb on - strand, within Pf1N1B4_1763 at 2016.668 kb on + strand, within Pf1N1B4_1763 at 2016.668 kb on + strand, within Pf1N1B4_1763 at 2016.685 kb on - strand, within Pf1N1B4_1763 at 2016.732 kb on + strand, within Pf1N1B4_1763 at 2016.744 kb on + strand, within Pf1N1B4_1763 at 2016.745 kb on - strand at 2016.785 kb on + strand, within Pf1N1B4_1763 at 2016.786 kb on - strand, within Pf1N1B4_1763 at 2016.786 kb on - strand, within Pf1N1B4_1763 at 2016.901 kb on + strand, within Pf1N1B4_1763 at 2017.062 kb on + strand, within Pf1N1B4_1763 at 2017.062 kb on + strand, within Pf1N1B4_1763 at 2017.062 kb on + strand, within Pf1N1B4_1763 at 2017.063 kb on - strand, within Pf1N1B4_1763 at 2017.063 kb on - strand, within Pf1N1B4_1763 at 2017.063 kb on - strand, within Pf1N1B4_1763
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 2,014,446 + Pf1N1B4_1761 0.34 -1.7 2,014,446 + Pf1N1B4_1761 0.34 -1.1 2,014,620 + Pf1N1B4_1761 0.49 -0.6 2,014,620 + Pf1N1B4_1761 0.49 +0.0 2,014,620 + Pf1N1B4_1761 0.49 +0.3 2,014,621 - Pf1N1B4_1761 0.50 -1.0 2,014,629 + Pf1N1B4_1761 0.50 +0.9 2,014,629 + Pf1N1B4_1761 0.50 -0.7 2,014,716 + Pf1N1B4_1761 0.58 -1.3 2,014,716 + Pf1N1B4_1761 0.58 -0.3 2,014,716 + Pf1N1B4_1761 0.58 -1.0 2,014,716 + Pf1N1B4_1761 0.58 -1.3 2,014,716 + Pf1N1B4_1761 0.58 +0.3 2,014,716 + -0.7 2,014,717 - Pf1N1B4_1761 0.58 -0.2 2,014,717 - Pf1N1B4_1761 0.58 -1.7 2,014,717 - Pf1N1B4_1761 0.58 -1.3 2,014,717 - Pf1N1B4_1761 0.58 -0.7 2,014,717 - Pf1N1B4_1761 0.58 -0.1 2,014,717 - Pf1N1B4_1761 0.58 -0.3 2,014,717 - Pf1N1B4_1761 0.58 -1.3 2,014,797 + Pf1N1B4_1761 0.65 -0.3 2,014,954 + Pf1N1B4_1761 0.80 -1.3 2,014,954 + Pf1N1B4_1761 0.80 -0.1 2,014,955 - Pf1N1B4_1761 0.80 -0.1 2,014,955 - Pf1N1B4_1761 0.80 +1.1 2,014,955 - Pf1N1B4_1761 0.80 -0.7 2,014,955 - Pf1N1B4_1761 0.80 +1.3 2,015,253 + -1.7 2,015,254 - -1.3 2,015,310 - -0.7 2,015,376 + Pf1N1B4_1762 0.17 -1.0 2,015,376 + Pf1N1B4_1762 0.17 +0.3 2,015,376 + Pf1N1B4_1762 0.17 -1.3 2,015,424 - Pf1N1B4_1762 0.23 +0.3 2,015,558 + Pf1N1B4_1762 0.39 -0.5 2,015,559 - Pf1N1B4_1762 0.39 -1.0 2,015,559 - Pf1N1B4_1762 0.39 -0.7 2,015,559 - Pf1N1B4_1762 0.39 -0.7 2,015,629 - Pf1N1B4_1762 0.47 +0.3 2,015,630 + Pf1N1B4_1762 0.47 -0.7 2,015,630 + Pf1N1B4_1762 0.47 -0.3 2,015,631 - Pf1N1B4_1762 0.47 +1.6 2,015,631 - Pf1N1B4_1762 0.47 +0.3 2,015,631 - Pf1N1B4_1762 0.47 -1.0 2,015,631 - Pf1N1B4_1762 0.47 -2.1 2,015,631 - Pf1N1B4_1762 0.47 -0.7 2,016,045 + -1.3 2,016,045 + +0.9 2,016,078 + -0.2 2,016,078 + -0.3 2,016,078 + +1.0 2,016,078 + -0.6 2,016,078 + -1.3 2,016,078 + -0.7 2,016,078 + -1.6 2,016,078 + -1.6 2,016,078 + -0.3 2,016,078 + +1.3 2,016,079 - +0.3 2,016,079 - -1.3 2,016,079 - +0.3 2,016,079 - -1.3 2,016,079 - -0.7 2,016,090 + -0.5 2,016,090 + +0.6 2,016,091 - -1.3 2,016,091 - -0.7 2,016,101 + -1.0 2,016,101 + -0.4 2,016,102 - +1.3 2,016,102 - +0.3 2,016,139 - -1.3 2,016,152 + +1.3 2,016,177 + +0.3 2,016,177 + -0.1 2,016,178 - -0.3 2,016,178 - +0.7 2,016,178 - -1.2 2,016,178 - -1.1 2,016,204 - -2.3 2,016,223 - -2.1 2,016,234 + +1.3 2,016,234 + -1.7 2,016,234 + +0.3 2,016,234 + +0.3 2,016,234 + -0.2 2,016,234 + +0.3 2,016,234 + -0.3 2,016,234 + -0.7 2,016,234 + +0.3 2,016,234 + +0.3 2,016,234 + -0.6 2,016,234 + -0.7 2,016,234 + -1.3 2,016,234 + -1.1 2,016,235 - -0.3 2,016,235 - -0.7 2,016,235 - -0.1 2,016,235 - +2.6 2,016,235 - +0.3 2,016,235 - +1.3 2,016,381 + Pf1N1B4_1763 0.10 -0.7 2,016,381 + Pf1N1B4_1763 0.10 +0.3 2,016,381 + Pf1N1B4_1763 0.10 +0.1 2,016,382 - Pf1N1B4_1763 0.10 +0.1 2,016,382 - Pf1N1B4_1763 0.10 -0.6 2,016,415 - Pf1N1B4_1763 0.12 -1.7 2,016,459 + Pf1N1B4_1763 0.15 -0.2 2,016,459 + Pf1N1B4_1763 0.15 -0.1 2,016,460 - Pf1N1B4_1763 0.15 -1.1 2,016,460 - Pf1N1B4_1763 0.15 +0.3 2,016,489 + Pf1N1B4_1763 0.17 +1.3 2,016,489 + Pf1N1B4_1763 0.17 -0.1 2,016,489 + Pf1N1B4_1763 0.17 -0.7 2,016,489 + Pf1N1B4_1763 0.17 -0.7 2,016,489 + +1.3 2,016,489 + Pf1N1B4_1763 0.17 -0.3 2,016,489 + Pf1N1B4_1763 0.17 +0.3 2,016,489 + Pf1N1B4_1763 0.17 +0.9 2,016,489 + Pf1N1B4_1763 0.17 +1.9 2,016,489 + Pf1N1B4_1763 0.17 +0.3 2,016,490 - Pf1N1B4_1763 0.17 -0.3 2,016,490 - Pf1N1B4_1763 0.17 -0.1 2,016,490 - Pf1N1B4_1763 0.17 +0.3 2,016,490 - Pf1N1B4_1763 0.17 +0.9 2,016,490 - Pf1N1B4_1763 0.17 +0.3 2,016,490 - Pf1N1B4_1763 0.17 +0.9 2,016,490 - Pf1N1B4_1763 0.17 +1.3 2,016,490 - Pf1N1B4_1763 0.17 -1.7 2,016,492 + Pf1N1B4_1763 0.17 +1.6 2,016,492 + Pf1N1B4_1763 0.17 -1.1 2,016,492 + Pf1N1B4_1763 0.17 -1.7 2,016,492 + Pf1N1B4_1763 0.17 +0.7 2,016,493 - Pf1N1B4_1763 0.17 -0.5 2,016,493 - Pf1N1B4_1763 0.17 -0.3 2,016,493 - Pf1N1B4_1763 0.17 -0.7 2,016,493 - Pf1N1B4_1763 0.17 -0.1 2,016,498 + Pf1N1B4_1763 0.18 -1.7 2,016,498 + Pf1N1B4_1763 0.18 -1.0 2,016,498 + Pf1N1B4_1763 0.18 -1.1 2,016,498 + Pf1N1B4_1763 0.18 +0.3 2,016,498 + Pf1N1B4_1763 0.18 -1.3 2,016,498 + Pf1N1B4_1763 0.18 +0.1 2,016,498 + Pf1N1B4_1763 0.18 -0.7 2,016,498 + Pf1N1B4_1763 0.18 -0.7 2,016,498 + +0.3 2,016,498 + Pf1N1B4_1763 0.18 -1.1 2,016,498 + Pf1N1B4_1763 0.18 +0.3 2,016,498 + Pf1N1B4_1763 0.18 -1.3 2,016,498 + Pf1N1B4_1763 0.18 -0.1 2,016,499 - Pf1N1B4_1763 0.18 -0.1 2,016,499 - Pf1N1B4_1763 0.18 -0.7 2,016,499 - Pf1N1B4_1763 0.18 -1.7 2,016,499 - Pf1N1B4_1763 0.18 -1.9 2,016,499 - Pf1N1B4_1763 0.18 -1.7 2,016,499 - Pf1N1B4_1763 0.18 +1.5 2,016,499 - Pf1N1B4_1763 0.18 -1.0 2,016,499 - Pf1N1B4_1763 0.18 -1.3 2,016,499 - Pf1N1B4_1763 0.18 +0.9 2,016,499 - Pf1N1B4_1763 0.18 +0.3 2,016,499 - -0.3 2,016,564 + Pf1N1B4_1763 0.22 +0.7 2,016,564 + Pf1N1B4_1763 0.22 -0.2 2,016,564 + Pf1N1B4_1763 0.22 -1.5 2,016,564 + Pf1N1B4_1763 0.22 -0.1 2,016,564 + Pf1N1B4_1763 0.22 -2.1 2,016,564 + Pf1N1B4_1763 0.22 +0.1 2,016,564 + Pf1N1B4_1763 0.22 -1.0 2,016,564 + Pf1N1B4_1763 0.22 +0.7 2,016,565 - Pf1N1B4_1763 0.22 +0.3 2,016,565 - Pf1N1B4_1763 0.22 -0.1 2,016,668 + Pf1N1B4_1763 0.29 -1.3 2,016,668 + Pf1N1B4_1763 0.29 +0.3 2,016,685 - Pf1N1B4_1763 0.30 -0.3 2,016,732 + Pf1N1B4_1763 0.33 -1.7 2,016,744 + Pf1N1B4_1763 0.33 -0.8 2,016,745 - +0.3 2,016,785 + Pf1N1B4_1763 0.36 +0.9 2,016,786 - Pf1N1B4_1763 0.36 +0.7 2,016,786 - Pf1N1B4_1763 0.36 +2.4 2,016,901 + Pf1N1B4_1763 0.43 -1.7 2,017,062 + Pf1N1B4_1763 0.54 -0.4 2,017,062 + Pf1N1B4_1763 0.54 -1.1 2,017,062 + Pf1N1B4_1763 0.54 -0.1 2,017,063 - Pf1N1B4_1763 0.54 +0.7 2,017,063 - Pf1N1B4_1763 0.54 -1.9 2,017,063 - Pf1N1B4_1763 0.54 +0.9
Or see this region's nucleotide sequence