Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_158 and Pf1N1B4_159 are separated by 26 nucleotides Pf1N1B4_159 and Pf1N1B4_160 are separated by 89 nucleotides Pf1N1B4_160 and Pf1N1B4_161 are separated by 198 nucleotides Pf1N1B4_161 and Pf1N1B4_162 are separated by 177 nucleotides
Pf1N1B4_158 - FIG00456158: hypothetical protein, at 200,783 to 201,112
_158
Pf1N1B4_159 - Putative threonine efflux protein, at 201,139 to 201,768
_159
Pf1N1B4_160 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 201,858 to 203,183
_160
Pf1N1B4_161 - hypothetical protein, at 203,382 to 203,504
_161
Pf1N1B4_162 - Aha1 domain superfamily, at 203,682 to 204,170
_162
Position (kb) 201
202
203
204 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 200.935 kb on + strand, within Pf1N1B4_158 at 200.935 kb on + strand, within Pf1N1B4_158 at 200.956 kb on - strand at 200.959 kb on - strand, within Pf1N1B4_158 at 200.959 kb on - strand, within Pf1N1B4_158 at 200.959 kb on - strand, within Pf1N1B4_158 at 201.090 kb on + strand at 201.091 kb on - strand at 201.095 kb on - strand at 201.108 kb on + strand at 201.108 kb on + strand at 201.108 kb on + strand at 201.108 kb on + strand at 201.108 kb on + strand at 201.109 kb on - strand at 201.109 kb on - strand at 201.231 kb on + strand at 201.255 kb on + strand, within Pf1N1B4_159 at 201.256 kb on - strand, within Pf1N1B4_159 at 201.392 kb on - strand, within Pf1N1B4_159 at 201.665 kb on + strand, within Pf1N1B4_159 at 201.666 kb on - strand, within Pf1N1B4_159 at 201.797 kb on - strand at 201.797 kb on - strand at 201.801 kb on + strand at 201.801 kb on + strand at 201.840 kb on + strand at 202.002 kb on + strand, within Pf1N1B4_160 at 202.003 kb on - strand at 202.168 kb on + strand, within Pf1N1B4_160 at 202.169 kb on - strand, within Pf1N1B4_160 at 202.280 kb on - strand, within Pf1N1B4_160 at 202.280 kb on - strand, within Pf1N1B4_160 at 202.407 kb on + strand, within Pf1N1B4_160 at 202.534 kb on - strand, within Pf1N1B4_160 at 202.647 kb on + strand, within Pf1N1B4_160 at 202.648 kb on - strand, within Pf1N1B4_160 at 202.685 kb on + strand, within Pf1N1B4_160 at 202.685 kb on + strand, within Pf1N1B4_160 at 202.686 kb on - strand, within Pf1N1B4_160 at 202.686 kb on - strand, within Pf1N1B4_160 at 202.710 kb on + strand, within Pf1N1B4_160 at 202.710 kb on + strand, within Pf1N1B4_160 at 202.711 kb on - strand, within Pf1N1B4_160 at 202.711 kb on - strand, within Pf1N1B4_160 at 202.711 kb on - strand, within Pf1N1B4_160 at 202.711 kb on - strand, within Pf1N1B4_160 at 202.711 kb on - strand, within Pf1N1B4_160 at 202.711 kb on - strand, within Pf1N1B4_160 at 202.711 kb on - strand, within Pf1N1B4_160 at 202.711 kb on - strand, within Pf1N1B4_160 at 202.711 kb on - strand at 202.711 kb on - strand, within Pf1N1B4_160 at 202.711 kb on - strand, within Pf1N1B4_160 at 202.713 kb on - strand, within Pf1N1B4_160 at 202.772 kb on + strand, within Pf1N1B4_160 at 202.773 kb on - strand, within Pf1N1B4_160 at 202.773 kb on - strand, within Pf1N1B4_160 at 202.773 kb on - strand, within Pf1N1B4_160 at 202.773 kb on - strand, within Pf1N1B4_160 at 202.977 kb on + strand, within Pf1N1B4_160 at 202.978 kb on - strand, within Pf1N1B4_160 at 202.978 kb on - strand, within Pf1N1B4_160 at 202.980 kb on + strand, within Pf1N1B4_160 at 202.980 kb on + strand, within Pf1N1B4_160 at 202.980 kb on + strand, within Pf1N1B4_160 at 202.980 kb on + strand, within Pf1N1B4_160 at 202.981 kb on - strand, within Pf1N1B4_160 at 202.981 kb on - strand, within Pf1N1B4_160 at 203.008 kb on + strand at 203.043 kb on - strand, within Pf1N1B4_160 at 203.269 kb on + strand at 203.327 kb on + strand at 203.328 kb on - strand at 203.328 kb on - strand at 203.359 kb on + strand at 203.359 kb on + strand at 203.360 kb on - strand at 203.360 kb on - strand at 203.360 kb on - strand at 203.369 kb on + strand at 203.370 kb on - strand at 203.375 kb on + strand at 203.375 kb on + strand at 203.376 kb on - strand at 203.487 kb on - strand, within Pf1N1B4_161 at 203.540 kb on + strand at 203.540 kb on + strand at 203.541 kb on - strand at 203.541 kb on - strand at 203.541 kb on - strand at 203.541 kb on - strand at 203.541 kb on - strand at 203.541 kb on - strand at 203.577 kb on + strand at 203.578 kb on - strand at 203.578 kb on - strand at 203.578 kb on - strand at 203.578 kb on - strand at 203.578 kb on - strand at 203.578 kb on - strand at 203.578 kb on - strand at 203.578 kb on - strand at 203.578 kb on - strand at 203.578 kb on - strand at 203.578 kb on - strand at 203.578 kb on - strand at 203.578 kb on - strand at 203.578 kb on - strand at 203.578 kb on - strand at 203.578 kb on - strand at 203.578 kb on - strand at 203.581 kb on + strand at 203.582 kb on - strand at 203.582 kb on - strand at 203.582 kb on - strand at 203.582 kb on - strand at 203.582 kb on - strand at 203.582 kb on - strand at 203.593 kb on + strand at 203.594 kb on - strand at 203.594 kb on - strand at 203.607 kb on + strand at 203.635 kb on + strand at 203.636 kb on - strand at 203.636 kb on - strand at 203.985 kb on + strand, within Pf1N1B4_162 at 203.986 kb on - strand, within Pf1N1B4_162 at 203.986 kb on - strand, within Pf1N1B4_162 at 204.047 kb on + strand, within Pf1N1B4_162 at 204.170 kb on + strand at 204.170 kb on + strand at 204.170 kb on + strand at 204.170 kb on + strand at 204.170 kb on + strand at 204.171 kb on - strand at 204.171 kb on - strand at 204.171 kb on - strand at 204.171 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 200,935 + Pf1N1B4_158 0.46 -0.2 200,935 + Pf1N1B4_158 0.46 -1.2 200,956 - +0.4 200,959 - Pf1N1B4_158 0.53 -0.8 200,959 - Pf1N1B4_158 0.53 -1.7 200,959 - Pf1N1B4_158 0.53 +0.1 201,090 + -0.2 201,091 - +0.2 201,095 - -0.2 201,108 + -0.2 201,108 + -1.4 201,108 + +1.4 201,108 + +0.8 201,108 + -0.4 201,109 - +1.8 201,109 - -0.2 201,231 + -0.2 201,255 + Pf1N1B4_159 0.18 +1.1 201,256 - Pf1N1B4_159 0.19 -2.8 201,392 - Pf1N1B4_159 0.40 -1.5 201,665 + Pf1N1B4_159 0.83 -0.6 201,666 - Pf1N1B4_159 0.84 +0.4 201,797 - -1.2 201,797 - +1.8 201,801 + -0.6 201,801 + -0.2 201,840 + -2.5 202,002 + Pf1N1B4_160 0.11 +0.2 202,003 - -0.2 202,168 + Pf1N1B4_160 0.23 -0.2 202,169 - Pf1N1B4_160 0.23 +0.4 202,280 - Pf1N1B4_160 0.32 +0.8 202,280 - Pf1N1B4_160 0.32 +0.8 202,407 + Pf1N1B4_160 0.41 -0.6 202,534 - Pf1N1B4_160 0.51 -1.2 202,647 + Pf1N1B4_160 0.60 -1.8 202,648 - Pf1N1B4_160 0.60 -1.2 202,685 + Pf1N1B4_160 0.62 +0.6 202,685 + Pf1N1B4_160 0.62 -0.8 202,686 - Pf1N1B4_160 0.62 -0.4 202,686 - Pf1N1B4_160 0.62 -1.0 202,710 + Pf1N1B4_160 0.64 -0.2 202,710 + Pf1N1B4_160 0.64 -0.5 202,711 - Pf1N1B4_160 0.64 -0.2 202,711 - Pf1N1B4_160 0.64 -0.2 202,711 - Pf1N1B4_160 0.64 +0.1 202,711 - Pf1N1B4_160 0.64 -0.8 202,711 - Pf1N1B4_160 0.64 -0.7 202,711 - Pf1N1B4_160 0.64 -0.2 202,711 - Pf1N1B4_160 0.64 -0.8 202,711 - Pf1N1B4_160 0.64 -1.2 202,711 - +0.4 202,711 - Pf1N1B4_160 0.64 +0.7 202,711 - Pf1N1B4_160 0.64 -0.2 202,713 - Pf1N1B4_160 0.64 -0.8 202,772 + Pf1N1B4_160 0.69 +1.1 202,773 - Pf1N1B4_160 0.69 -0.9 202,773 - Pf1N1B4_160 0.69 +0.8 202,773 - Pf1N1B4_160 0.69 -0.6 202,773 - Pf1N1B4_160 0.69 -1.8 202,977 + Pf1N1B4_160 0.84 -0.2 202,978 - Pf1N1B4_160 0.84 -0.8 202,978 - Pf1N1B4_160 0.84 -0.6 202,980 + Pf1N1B4_160 0.85 +1.4 202,980 + Pf1N1B4_160 0.85 -0.8 202,980 + Pf1N1B4_160 0.85 +0.1 202,980 + Pf1N1B4_160 0.85 -2.8 202,981 - Pf1N1B4_160 0.85 -0.2 202,981 - Pf1N1B4_160 0.85 -1.8 203,008 + -0.2 203,043 - Pf1N1B4_160 0.89 -1.8 203,269 + +0.4 203,327 + +0.4 203,328 - -1.2 203,328 - -0.4 203,359 + +1.4 203,359 + +1.2 203,360 - -1.5 203,360 - -1.0 203,360 - -1.2 203,369 + +2.6 203,370 - -1.2 203,375 + -0.2 203,375 + -0.2 203,376 - +2.1 203,487 - Pf1N1B4_161 0.85 -1.4 203,540 + -0.2 203,540 + +0.8 203,541 - -0.2 203,541 - -0.2 203,541 - -0.2 203,541 - +0.4 203,541 - -0.4 203,541 - -0.2 203,577 + +0.6 203,578 - -1.8 203,578 - -1.8 203,578 - -0.6 203,578 - +2.1 203,578 - +0.6 203,578 - +0.6 203,578 - +0.1 203,578 - -1.8 203,578 - +1.4 203,578 - -0.2 203,578 - +0.8 203,578 - -1.2 203,578 - +0.6 203,578 - -1.2 203,578 - -1.2 203,578 - -1.2 203,578 - -0.6 203,581 + -0.2 203,582 - -0.8 203,582 - -1.8 203,582 - -1.1 203,582 - -1.8 203,582 - +1.5 203,582 - -0.7 203,593 + -1.8 203,594 - -0.6 203,594 - -0.2 203,607 + -0.3 203,635 + +0.4 203,636 - -0.2 203,636 - -1.2 203,985 + Pf1N1B4_162 0.62 -0.2 203,986 - Pf1N1B4_162 0.62 +0.4 203,986 - Pf1N1B4_162 0.62 +0.4 204,047 + Pf1N1B4_162 0.75 +0.8 204,170 + -1.2 204,170 + -0.2 204,170 + -2.5 204,170 + -3.0 204,170 + +0.2 204,171 - +2.0 204,171 - -0.3 204,171 - -0.6 204,171 - -0.2
Or see this region's nucleotide sequence