Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08260

Experiment: D-Raffinose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08255 and BWI76_RS08260 are separated by 97 nucleotidesBWI76_RS08260 and BWI76_RS08265 are separated by 129 nucleotidesBWI76_RS08265 and BWI76_RS08270 are separated by 291 nucleotides BWI76_RS08255: BWI76_RS08255 - tricarballylate dehydrogenase, at 1,747,578 to 1,748,981 _RS08255 BWI76_RS08260: BWI76_RS08260 - LysR family transcriptional regulator, at 1,749,079 to 1,750,005 _RS08260 BWI76_RS08265: BWI76_RS08265 - transcriptional repressor, at 1,750,135 to 1,750,587 _RS08265 BWI76_RS08270: BWI76_RS08270 - flavodoxin, at 1,750,879 to 1,751,508 _RS08270 Position (kb) 1749 1750 1751Strain fitness (log2 ratio) -1 0 1at 1748.208 kb on + strand, within BWI76_RS08255at 1748.216 kb on - strand, within BWI76_RS08255at 1748.299 kb on + strand, within BWI76_RS08255at 1748.336 kb on + strand, within BWI76_RS08255at 1748.344 kb on - strand, within BWI76_RS08255at 1748.584 kb on + strand, within BWI76_RS08255at 1748.660 kb on + strand, within BWI76_RS08255at 1748.666 kb on + strand, within BWI76_RS08255at 1749.594 kb on + strand, within BWI76_RS08260at 1749.845 kb on - strand, within BWI76_RS08260at 1750.030 kb on - strandat 1750.044 kb on + strandat 1750.688 kb on + strandat 1750.810 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Raffinose (C)
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1,748,208 + BWI76_RS08255 0.45 -0.5
1,748,216 - BWI76_RS08255 0.45 -0.1
1,748,299 + BWI76_RS08255 0.51 +0.4
1,748,336 + BWI76_RS08255 0.54 -0.8
1,748,344 - BWI76_RS08255 0.55 +0.4
1,748,584 + BWI76_RS08255 0.72 +1.1
1,748,660 + BWI76_RS08255 0.77 -0.1
1,748,666 + BWI76_RS08255 0.77 -0.4
1,749,594 + BWI76_RS08260 0.56 -1.4
1,749,845 - BWI76_RS08260 0.83 -1.0
1,750,030 - -0.6
1,750,044 + +0.2
1,750,688 + -0.4
1,750,810 + -0.9

Or see this region's nucleotide sequence