Experiment: D-Raffinose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02965 and BWI76_RS02970 are separated by 131 nucleotides BWI76_RS02970 and BWI76_RS02975 are separated by 37 nucleotides BWI76_RS02975 and BWI76_RS02980 are separated by 145 nucleotides
BWI76_RS02965: BWI76_RS02965 - PTS trehalose transporter subunit IIBC, at 607,360 to 608,778
_RS02965
BWI76_RS02970: BWI76_RS02970 - trehalose operon repressor, at 608,910 to 609,857
_RS02970
BWI76_RS02975: BWI76_RS02975 - hypothetical protein, at 609,895 to 610,091
_RS02975
BWI76_RS02980: BWI76_RS02980 - type I magnesium transporting ATPase, at 610,237 to 612,945
_RS02980
Position (kb) 608
609
610 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 607.912 kb on + strand, within BWI76_RS02965 at 607.915 kb on - strand, within BWI76_RS02965 at 608.178 kb on + strand, within BWI76_RS02965 at 608.408 kb on + strand, within BWI76_RS02965 at 608.534 kb on + strand, within BWI76_RS02965 at 608.549 kb on + strand at 608.576 kb on - strand, within BWI76_RS02965 at 608.585 kb on + strand, within BWI76_RS02965 at 608.585 kb on + strand, within BWI76_RS02965 at 608.698 kb on - strand at 608.792 kb on - strand at 608.945 kb on + strand at 609.101 kb on + strand, within BWI76_RS02970 at 609.396 kb on - strand, within BWI76_RS02970 at 609.488 kb on + strand, within BWI76_RS02970 at 609.617 kb on - strand, within BWI76_RS02970 at 609.668 kb on - strand, within BWI76_RS02970 at 609.888 kb on - strand at 609.890 kb on + strand at 609.898 kb on - strand at 610.134 kb on + strand at 610.197 kb on - strand at 610.303 kb on - strand at 610.567 kb on + strand, within BWI76_RS02980 at 610.852 kb on - strand, within BWI76_RS02980
Per-strain Table
Position Strand Gene LocusTag Fraction D-Raffinose (C) remove 607,912 + BWI76_RS02965 0.39 -3.8 607,915 - BWI76_RS02965 0.39 -3.5 608,178 + BWI76_RS02965 0.58 -3.1 608,408 + BWI76_RS02965 0.74 -4.4 608,534 + BWI76_RS02965 0.83 -3.3 608,549 + +1.3 608,576 - BWI76_RS02965 0.86 -3.3 608,585 + BWI76_RS02965 0.86 -1.5 608,585 + BWI76_RS02965 0.86 -2.8 608,698 - -1.5 608,792 - -0.7 608,945 + +1.2 609,101 + BWI76_RS02970 0.20 +0.6 609,396 - BWI76_RS02970 0.51 -0.7 609,488 + BWI76_RS02970 0.61 +1.0 609,617 - BWI76_RS02970 0.75 +0.6 609,668 - BWI76_RS02970 0.80 +0.9 609,888 - -0.0 609,890 + +0.5 609,898 - -1.0 610,134 + -0.6 610,197 - -0.4 610,303 - +0.1 610,567 + BWI76_RS02980 0.12 -0.5 610,852 - BWI76_RS02980 0.23 +0.6
Or see this region's nucleotide sequence