Experiment: 1-ethyl-3-methylimidazolium chloride 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2157 and Shewana3_2158 are separated by 132 nucleotides Shewana3_2158 and Shewana3_2159 are separated by 27 nucleotides Shewana3_2159 and Shewana3_2160 are separated by 79 nucleotides
Shewana3_2157: Shewana3_2157 - Smr protein/MutS2 (RefSeq), at 2,586,084 to 2,586,674
_2157
Shewana3_2158: Shewana3_2158 - hypothetical protein (RefSeq), at 2,586,807 to 2,587,385
_2158
Shewana3_2159: Shewana3_2159 - SecC motif-containing protein (RefSeq), at 2,587,413 to 2,587,907
_2159
Shewana3_2160: Shewana3_2160 - diguanylate cyclase/phosphodiesterase with PAS/PAC and GAF sensor(s) (RefSeq), at 2,587,987 to 2,590,170
_2160
Position (kb)
2587
2588 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2586.418 kb on + strand, within Shewana3_2157 at 2586.613 kb on + strand, within Shewana3_2157 at 2587.516 kb on + strand, within Shewana3_2159 at 2587.615 kb on + strand, within Shewana3_2159 at 2587.623 kb on - strand, within Shewana3_2159 at 2587.623 kb on - strand, within Shewana3_2159 at 2587.635 kb on + strand, within Shewana3_2159 at 2587.651 kb on + strand, within Shewana3_2159 at 2587.681 kb on + strand, within Shewana3_2159 at 2587.895 kb on + strand at 2588.416 kb on - strand, within Shewana3_2160 at 2588.416 kb on - strand, within Shewana3_2160 at 2588.798 kb on + strand, within Shewana3_2160
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 20 mM remove 2,586,418 + Shewana3_2157 0.57 +0.7 2,586,613 + Shewana3_2157 0.90 +0.7 2,587,516 + Shewana3_2159 0.21 +0.7 2,587,615 + Shewana3_2159 0.41 -0.3 2,587,623 - Shewana3_2159 0.42 +0.7 2,587,623 - Shewana3_2159 0.42 +0.7 2,587,635 + Shewana3_2159 0.45 +3.0 2,587,651 + Shewana3_2159 0.48 -1.3 2,587,681 + Shewana3_2159 0.54 +1.3 2,587,895 + +0.5 2,588,416 - Shewana3_2160 0.20 +0.7 2,588,416 - Shewana3_2160 0.20 +0.7 2,588,798 + Shewana3_2160 0.37 +0.7
Or see this region's nucleotide sequence