Experiment: 1-ethyl-3-methylimidazolium chloride 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0700 and rpsF are separated by 212 nucleotides rpsF and Shewana3_0702 are separated by 23 nucleotides Shewana3_0702 and rpsR are separated by 11 nucleotides rpsR and rplI are separated by 36 nucleotides
Shewana3_0700: Shewana3_0700 - hypothetical protein (RefSeq), at 817,318 to 818,073
_0700
Shewana3_0701: rpsF - 30S ribosomal protein S6 (RefSeq), at 818,286 to 818,681
rpsF
Shewana3_0702: Shewana3_0702 - primosomal replication protein N (RefSeq), at 818,705 to 818,995
_0702
Shewana3_0703: rpsR - 30S ribosomal protein S18 (RefSeq), at 819,007 to 819,234
rpsR
Shewana3_0704: rplI - 50S ribosomal protein L9 (RefSeq), at 819,271 to 819,723
rplI
Position (kb) 818
819 Strain fitness (log2 ratio) -1
0
1 at 817.338 kb on + strand at 817.338 kb on + strand at 817.346 kb on - strand at 817.346 kb on - strand at 817.346 kb on - strand at 817.373 kb on - strand at 817.649 kb on + strand, within Shewana3_0700 at 817.763 kb on + strand, within Shewana3_0700 at 817.765 kb on - strand, within Shewana3_0700 at 817.771 kb on - strand, within Shewana3_0700 at 817.773 kb on - strand, within Shewana3_0700 at 817.814 kb on - strand, within Shewana3_0700 at 817.824 kb on - strand, within Shewana3_0700 at 817.902 kb on + strand, within Shewana3_0700 at 817.933 kb on + strand, within Shewana3_0700 at 819.451 kb on + strand, within rplI
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 20 mM remove 817,338 + -0.3 817,338 + +1.4 817,346 - -0.3 817,346 - -0.1 817,346 - -0.0 817,373 - -0.5 817,649 + Shewana3_0700 0.44 -0.1 817,763 + Shewana3_0700 0.59 -0.5 817,765 - Shewana3_0700 0.59 +0.3 817,771 - Shewana3_0700 0.60 -0.8 817,773 - Shewana3_0700 0.60 -0.8 817,814 - Shewana3_0700 0.66 -0.1 817,824 - Shewana3_0700 0.67 -0.1 817,902 + Shewana3_0700 0.77 -0.0 817,933 + Shewana3_0700 0.81 -0.5 819,451 + rplI Shewana3_0704 0.40 -0.9
Or see this region's nucleotide sequence