Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_803 and Pf1N1B4_804 are separated by 144 nucleotides Pf1N1B4_804 and Pf1N1B4_805 are separated by 34 nucleotides
Pf1N1B4_803 - Membrane carboxypeptidase (penicillin-binding protein), at 916,810 to 919,224
_803
Pf1N1B4_804 - hypothetical protein, at 919,369 to 921,210
_804
Pf1N1B4_805 - hypothetical protein, at 921,245 to 922,516
_805
Position (kb)
919
920
921
922 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 918.409 kb on - strand, within Pf1N1B4_803 at 918.469 kb on + strand, within Pf1N1B4_803 at 918.470 kb on - strand, within Pf1N1B4_803 at 918.470 kb on - strand, within Pf1N1B4_803 at 918.732 kb on + strand, within Pf1N1B4_803 at 918.732 kb on + strand, within Pf1N1B4_803 at 918.733 kb on - strand, within Pf1N1B4_803 at 919.003 kb on + strand at 919.003 kb on + strand at 919.003 kb on + strand at 919.003 kb on + strand at 919.003 kb on + strand at 919.003 kb on + strand at 919.004 kb on - strand at 919.004 kb on - strand at 919.004 kb on - strand at 919.004 kb on - strand at 919.004 kb on - strand at 919.004 kb on - strand at 919.004 kb on - strand at 919.004 kb on - strand at 919.004 kb on - strand at 919.015 kb on - strand at 919.039 kb on + strand at 919.039 kb on + strand at 919.039 kb on + strand at 919.040 kb on - strand at 919.040 kb on - strand at 919.040 kb on - strand at 919.129 kb on + strand at 919.130 kb on - strand at 919.232 kb on + strand at 919.232 kb on + strand at 919.233 kb on - strand at 919.233 kb on - strand at 919.233 kb on - strand at 919.241 kb on - strand at 919.259 kb on - strand at 919.299 kb on + strand at 919.299 kb on + strand at 919.300 kb on - strand at 919.332 kb on - strand at 919.502 kb on + strand at 919.503 kb on - strand at 919.698 kb on + strand, within Pf1N1B4_804 at 919.698 kb on + strand, within Pf1N1B4_804 at 919.711 kb on + strand, within Pf1N1B4_804 at 919.808 kb on + strand, within Pf1N1B4_804 at 920.188 kb on + strand at 920.188 kb on + strand, within Pf1N1B4_804 at 920.188 kb on + strand, within Pf1N1B4_804 at 920.188 kb on + strand, within Pf1N1B4_804 at 920.189 kb on - strand, within Pf1N1B4_804 at 920.189 kb on - strand, within Pf1N1B4_804 at 920.189 kb on - strand, within Pf1N1B4_804 at 920.189 kb on - strand, within Pf1N1B4_804 at 920.189 kb on - strand, within Pf1N1B4_804 at 920.189 kb on - strand at 920.189 kb on - strand, within Pf1N1B4_804 at 920.191 kb on + strand at 920.191 kb on + strand, within Pf1N1B4_804 at 920.191 kb on + strand, within Pf1N1B4_804 at 920.191 kb on + strand, within Pf1N1B4_804 at 920.191 kb on + strand, within Pf1N1B4_804 at 920.191 kb on + strand, within Pf1N1B4_804 at 920.192 kb on - strand, within Pf1N1B4_804 at 920.192 kb on - strand, within Pf1N1B4_804 at 920.192 kb on - strand, within Pf1N1B4_804 at 920.192 kb on - strand, within Pf1N1B4_804 at 920.192 kb on - strand, within Pf1N1B4_804 at 920.192 kb on - strand, within Pf1N1B4_804 at 920.192 kb on - strand, within Pf1N1B4_804 at 920.284 kb on + strand, within Pf1N1B4_804 at 920.284 kb on + strand, within Pf1N1B4_804 at 920.284 kb on + strand, within Pf1N1B4_804 at 920.284 kb on + strand, within Pf1N1B4_804 at 920.285 kb on - strand, within Pf1N1B4_804 at 920.285 kb on - strand, within Pf1N1B4_804 at 920.285 kb on - strand, within Pf1N1B4_804 at 920.285 kb on - strand, within Pf1N1B4_804 at 920.596 kb on + strand, within Pf1N1B4_804 at 920.642 kb on - strand, within Pf1N1B4_804 at 920.833 kb on + strand, within Pf1N1B4_804 at 920.833 kb on + strand, within Pf1N1B4_804 at 920.834 kb on - strand, within Pf1N1B4_804 at 920.834 kb on - strand, within Pf1N1B4_804 at 920.918 kb on + strand, within Pf1N1B4_804 at 920.918 kb on + strand, within Pf1N1B4_804 at 920.918 kb on + strand, within Pf1N1B4_804 at 920.918 kb on + strand, within Pf1N1B4_804 at 920.919 kb on - strand, within Pf1N1B4_804 at 920.919 kb on - strand, within Pf1N1B4_804 at 920.919 kb on - strand at 920.919 kb on - strand, within Pf1N1B4_804 at 920.919 kb on - strand at 920.919 kb on - strand, within Pf1N1B4_804 at 921.416 kb on + strand, within Pf1N1B4_805 at 921.416 kb on + strand, within Pf1N1B4_805 at 921.416 kb on + strand, within Pf1N1B4_805 at 921.416 kb on + strand, within Pf1N1B4_805 at 921.416 kb on + strand, within Pf1N1B4_805 at 921.417 kb on - strand, within Pf1N1B4_805 at 921.417 kb on - strand, within Pf1N1B4_805 at 921.476 kb on + strand, within Pf1N1B4_805 at 921.476 kb on + strand, within Pf1N1B4_805 at 921.476 kb on + strand, within Pf1N1B4_805 at 921.476 kb on + strand, within Pf1N1B4_805 at 921.477 kb on - strand, within Pf1N1B4_805 at 921.645 kb on - strand, within Pf1N1B4_805 at 921.824 kb on + strand, within Pf1N1B4_805 at 921.824 kb on + strand, within Pf1N1B4_805 at 921.824 kb on + strand, within Pf1N1B4_805 at 921.824 kb on + strand, within Pf1N1B4_805 at 921.825 kb on - strand, within Pf1N1B4_805 at 921.838 kb on + strand, within Pf1N1B4_805 at 921.839 kb on - strand, within Pf1N1B4_805 at 922.015 kb on + strand, within Pf1N1B4_805 at 922.016 kb on - strand, within Pf1N1B4_805 at 922.016 kb on - strand, within Pf1N1B4_805 at 922.127 kb on - strand, within Pf1N1B4_805 at 922.127 kb on - strand, within Pf1N1B4_805 at 922.149 kb on + strand, within Pf1N1B4_805 at 922.149 kb on + strand, within Pf1N1B4_805 at 922.149 kb on + strand, within Pf1N1B4_805 at 922.149 kb on + strand, within Pf1N1B4_805 at 922.149 kb on + strand, within Pf1N1B4_805 at 922.150 kb on - strand, within Pf1N1B4_805
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 918,409 - Pf1N1B4_803 0.66 +0.2 918,469 + Pf1N1B4_803 0.69 +0.1 918,470 - Pf1N1B4_803 0.69 +1.0 918,470 - Pf1N1B4_803 0.69 -0.3 918,732 + Pf1N1B4_803 0.80 -0.5 918,732 + Pf1N1B4_803 0.80 -0.0 918,733 - Pf1N1B4_803 0.80 -1.0 919,003 + -0.0 919,003 + -3.0 919,003 + +0.6 919,003 + +0.7 919,003 + -0.0 919,003 + -1.6 919,004 - +1.3 919,004 - +1.0 919,004 - +0.4 919,004 - -0.6 919,004 - -1.6 919,004 - -1.0 919,004 - -0.0 919,004 - +0.4 919,004 - +0.6 919,015 - +2.6 919,039 + -0.8 919,039 + -0.6 919,039 + -0.3 919,040 - +2.3 919,040 - -0.0 919,040 - -0.3 919,129 + +0.7 919,130 - +0.6 919,232 + +0.3 919,232 + +0.6 919,233 - -0.5 919,233 - -0.0 919,233 - -2.0 919,241 - +0.4 919,259 - -0.0 919,299 + -0.6 919,299 + -0.7 919,300 - +0.6 919,332 - -1.0 919,502 + +0.3 919,503 - +0.7 919,698 + Pf1N1B4_804 0.18 +1.6 919,698 + Pf1N1B4_804 0.18 -0.7 919,711 + Pf1N1B4_804 0.19 -1.6 919,808 + Pf1N1B4_804 0.24 +0.3 920,188 + -0.6 920,188 + Pf1N1B4_804 0.44 +0.8 920,188 + Pf1N1B4_804 0.44 -0.0 920,188 + Pf1N1B4_804 0.44 -0.4 920,189 - Pf1N1B4_804 0.45 +0.4 920,189 - Pf1N1B4_804 0.45 +0.2 920,189 - Pf1N1B4_804 0.45 +1.1 920,189 - Pf1N1B4_804 0.45 -0.8 920,189 - Pf1N1B4_804 0.45 -0.5 920,189 - -0.0 920,189 - Pf1N1B4_804 0.45 -0.2 920,191 + +1.0 920,191 + Pf1N1B4_804 0.45 -0.6 920,191 + Pf1N1B4_804 0.45 -0.0 920,191 + Pf1N1B4_804 0.45 +1.3 920,191 + Pf1N1B4_804 0.45 +0.6 920,191 + Pf1N1B4_804 0.45 -0.2 920,192 - Pf1N1B4_804 0.45 -0.0 920,192 - Pf1N1B4_804 0.45 -1.3 920,192 - Pf1N1B4_804 0.45 -2.6 920,192 - Pf1N1B4_804 0.45 -0.6 920,192 - Pf1N1B4_804 0.45 -0.6 920,192 - Pf1N1B4_804 0.45 +2.0 920,192 - Pf1N1B4_804 0.45 +1.0 920,284 + Pf1N1B4_804 0.50 +1.3 920,284 + Pf1N1B4_804 0.50 +1.0 920,284 + Pf1N1B4_804 0.50 -1.9 920,284 + Pf1N1B4_804 0.50 +2.0 920,285 - Pf1N1B4_804 0.50 +1.0 920,285 - Pf1N1B4_804 0.50 -0.0 920,285 - Pf1N1B4_804 0.50 +1.6 920,285 - Pf1N1B4_804 0.50 -0.0 920,596 + Pf1N1B4_804 0.67 +1.0 920,642 - Pf1N1B4_804 0.69 -0.3 920,833 + Pf1N1B4_804 0.79 +0.3 920,833 + Pf1N1B4_804 0.79 -0.8 920,834 - Pf1N1B4_804 0.80 +0.9 920,834 - Pf1N1B4_804 0.80 +0.2 920,918 + Pf1N1B4_804 0.84 -1.0 920,918 + Pf1N1B4_804 0.84 +0.4 920,918 + Pf1N1B4_804 0.84 -0.0 920,918 + Pf1N1B4_804 0.84 -0.0 920,919 - Pf1N1B4_804 0.84 +0.6 920,919 - Pf1N1B4_804 0.84 +1.0 920,919 - +1.0 920,919 - Pf1N1B4_804 0.84 -0.1 920,919 - -0.0 920,919 - Pf1N1B4_804 0.84 -1.0 921,416 + Pf1N1B4_805 0.13 +0.6 921,416 + Pf1N1B4_805 0.13 -0.4 921,416 + Pf1N1B4_805 0.13 -0.3 921,416 + Pf1N1B4_805 0.13 +1.0 921,416 + Pf1N1B4_805 0.13 -1.4 921,417 - Pf1N1B4_805 0.14 -0.3 921,417 - Pf1N1B4_805 0.14 -0.2 921,476 + Pf1N1B4_805 0.18 -0.0 921,476 + Pf1N1B4_805 0.18 +2.0 921,476 + Pf1N1B4_805 0.18 +1.3 921,476 + Pf1N1B4_805 0.18 -1.0 921,477 - Pf1N1B4_805 0.18 -0.6 921,645 - Pf1N1B4_805 0.31 -0.6 921,824 + Pf1N1B4_805 0.46 -0.0 921,824 + Pf1N1B4_805 0.46 +0.4 921,824 + Pf1N1B4_805 0.46 -0.0 921,824 + Pf1N1B4_805 0.46 -1.0 921,825 - Pf1N1B4_805 0.46 +0.3 921,838 + Pf1N1B4_805 0.47 -0.3 921,839 - Pf1N1B4_805 0.47 -0.5 922,015 + Pf1N1B4_805 0.61 +1.0 922,016 - Pf1N1B4_805 0.61 -1.0 922,016 - Pf1N1B4_805 0.61 +1.6 922,127 - Pf1N1B4_805 0.69 -0.0 922,127 - Pf1N1B4_805 0.69 -0.0 922,149 + Pf1N1B4_805 0.71 +1.0 922,149 + Pf1N1B4_805 0.71 -0.0 922,149 + Pf1N1B4_805 0.71 -0.0 922,149 + Pf1N1B4_805 0.71 +1.0 922,149 + Pf1N1B4_805 0.71 +1.9 922,150 - Pf1N1B4_805 0.71 +0.4
Or see this region's nucleotide sequence