Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_603 and Pf1N1B4_604 are separated by 77 nucleotides Pf1N1B4_604 and Pf1N1B4_605 are separated by 28 nucleotides Pf1N1B4_605 and Pf1N1B4_606 overlap by 69 nucleotides Pf1N1B4_606 and Pf1N1B4_607 are separated by 122 nucleotides
Pf1N1B4_603 - Methylglyoxal synthase (EC 4.2.3.3), at 697,008 to 697,472
_603
Pf1N1B4_604 - FIG006045: Sigma factor, ECF subfamily, at 697,550 to 698,068
_604
Pf1N1B4_605 - hypothetical protein, at 698,097 to 698,288
_605
Pf1N1B4_606 - Iron siderophore sensor protein, at 698,220 to 699,128
_606
Pf1N1B4_607 - TonB-dependent hemin , ferrichrome receptor, at 699,251 to 701,812
_607
Position (kb)
698
699
700 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 697.389 kb on + strand, within Pf1N1B4_603 at 697.389 kb on + strand, within Pf1N1B4_603 at 697.389 kb on + strand, within Pf1N1B4_603 at 697.390 kb on - strand, within Pf1N1B4_603 at 697.390 kb on - strand, within Pf1N1B4_603 at 697.400 kb on + strand, within Pf1N1B4_603 at 697.400 kb on + strand, within Pf1N1B4_603 at 697.401 kb on - strand, within Pf1N1B4_603 at 697.401 kb on - strand, within Pf1N1B4_603 at 697.444 kb on - strand at 697.452 kb on + strand at 697.452 kb on + strand at 697.452 kb on + strand at 697.452 kb on + strand at 697.453 kb on - strand at 697.453 kb on - strand at 697.453 kb on - strand at 697.471 kb on - strand at 697.664 kb on + strand, within Pf1N1B4_604 at 697.665 kb on - strand, within Pf1N1B4_604 at 697.665 kb on - strand, within Pf1N1B4_604 at 697.958 kb on + strand, within Pf1N1B4_604 at 697.958 kb on + strand, within Pf1N1B4_604 at 697.958 kb on + strand, within Pf1N1B4_604 at 697.958 kb on + strand, within Pf1N1B4_604 at 697.959 kb on - strand, within Pf1N1B4_604 at 697.959 kb on - strand, within Pf1N1B4_604 at 697.959 kb on - strand, within Pf1N1B4_604 at 697.959 kb on - strand, within Pf1N1B4_604 at 698.098 kb on + strand at 698.098 kb on + strand at 698.635 kb on - strand, within Pf1N1B4_606 at 698.840 kb on + strand, within Pf1N1B4_606 at 698.840 kb on + strand, within Pf1N1B4_606 at 698.974 kb on - strand, within Pf1N1B4_606 at 699.030 kb on - strand, within Pf1N1B4_606 at 699.219 kb on + strand at 699.219 kb on + strand at 699.219 kb on + strand at 699.220 kb on - strand at 699.220 kb on - strand at 699.220 kb on - strand at 699.222 kb on - strand at 699.222 kb on - strand at 699.294 kb on + strand at 699.294 kb on + strand at 699.294 kb on + strand at 699.294 kb on + strand at 699.295 kb on - strand at 699.295 kb on - strand at 699.295 kb on - strand at 699.386 kb on + strand at 699.386 kb on + strand at 699.584 kb on - strand, within Pf1N1B4_607 at 699.737 kb on - strand at 699.813 kb on - strand, within Pf1N1B4_607 at 699.897 kb on - strand, within Pf1N1B4_607 at 699.917 kb on + strand, within Pf1N1B4_607 at 699.917 kb on + strand, within Pf1N1B4_607 at 699.917 kb on + strand, within Pf1N1B4_607 at 699.917 kb on + strand, within Pf1N1B4_607 at 699.917 kb on + strand, within Pf1N1B4_607 at 699.917 kb on + strand, within Pf1N1B4_607 at 699.917 kb on + strand, within Pf1N1B4_607 at 699.917 kb on + strand, within Pf1N1B4_607 at 699.917 kb on + strand at 699.918 kb on - strand at 699.918 kb on - strand at 699.918 kb on - strand, within Pf1N1B4_607 at 699.918 kb on - strand, within Pf1N1B4_607 at 699.918 kb on - strand, within Pf1N1B4_607 at 699.918 kb on - strand, within Pf1N1B4_607 at 699.975 kb on + strand, within Pf1N1B4_607 at 699.976 kb on - strand, within Pf1N1B4_607 at 699.978 kb on - strand, within Pf1N1B4_607 at 700.011 kb on - strand, within Pf1N1B4_607 at 700.011 kb on - strand, within Pf1N1B4_607 at 700.011 kb on - strand, within Pf1N1B4_607 at 700.013 kb on + strand, within Pf1N1B4_607 at 700.013 kb on + strand, within Pf1N1B4_607 at 700.013 kb on + strand, within Pf1N1B4_607 at 700.013 kb on + strand, within Pf1N1B4_607 at 700.014 kb on - strand, within Pf1N1B4_607 at 700.014 kb on - strand, within Pf1N1B4_607 at 700.014 kb on - strand, within Pf1N1B4_607 at 700.014 kb on - strand, within Pf1N1B4_607 at 700.014 kb on - strand, within Pf1N1B4_607 at 700.079 kb on + strand, within Pf1N1B4_607
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 697,389 + Pf1N1B4_603 0.82 -0.7 697,389 + Pf1N1B4_603 0.82 +0.5 697,389 + Pf1N1B4_603 0.82 +0.9 697,390 - Pf1N1B4_603 0.82 +0.9 697,390 - Pf1N1B4_603 0.82 +1.9 697,400 + Pf1N1B4_603 0.84 -2.1 697,400 + Pf1N1B4_603 0.84 -0.8 697,401 - Pf1N1B4_603 0.85 -0.7 697,401 - Pf1N1B4_603 0.85 +1.3 697,444 - +0.5 697,452 + -0.1 697,452 + -2.1 697,452 + -0.7 697,452 + +1.7 697,453 - -1.7 697,453 - +1.0 697,453 - +0.5 697,471 - +0.7 697,664 + Pf1N1B4_604 0.22 -0.4 697,665 - Pf1N1B4_604 0.22 +1.5 697,665 - Pf1N1B4_604 0.22 +0.8 697,958 + Pf1N1B4_604 0.79 -2.2 697,958 + Pf1N1B4_604 0.79 +0.3 697,958 + Pf1N1B4_604 0.79 -0.1 697,958 + Pf1N1B4_604 0.79 -0.8 697,959 - Pf1N1B4_604 0.79 -1.9 697,959 - Pf1N1B4_604 0.79 +0.3 697,959 - Pf1N1B4_604 0.79 -0.1 697,959 - Pf1N1B4_604 0.79 +0.3 698,098 + +0.5 698,098 + -0.1 698,635 - Pf1N1B4_606 0.46 +0.3 698,840 + Pf1N1B4_606 0.68 -2.1 698,840 + Pf1N1B4_606 0.68 +0.7 698,974 - Pf1N1B4_606 0.83 -1.7 699,030 - Pf1N1B4_606 0.89 -2.1 699,219 + -0.5 699,219 + +0.9 699,219 + -1.1 699,220 - -0.3 699,220 - +0.7 699,220 - -1.7 699,222 - -0.1 699,222 - +0.9 699,294 + -0.9 699,294 + -1.1 699,294 + +0.1 699,294 + -0.4 699,295 - -0.8 699,295 - -1.7 699,295 - -0.5 699,386 + -1.1 699,386 + -0.5 699,584 - Pf1N1B4_607 0.13 -0.3 699,737 - +0.9 699,813 - Pf1N1B4_607 0.22 +0.7 699,897 - Pf1N1B4_607 0.25 +1.3 699,917 + Pf1N1B4_607 0.26 +1.9 699,917 + Pf1N1B4_607 0.26 +0.7 699,917 + Pf1N1B4_607 0.26 +0.9 699,917 + Pf1N1B4_607 0.26 +0.9 699,917 + Pf1N1B4_607 0.26 +1.5 699,917 + Pf1N1B4_607 0.26 -1.4 699,917 + Pf1N1B4_607 0.26 +1.3 699,917 + Pf1N1B4_607 0.26 +0.4 699,917 + -1.6 699,918 - -0.1 699,918 - -1.6 699,918 - Pf1N1B4_607 0.26 -1.3 699,918 - Pf1N1B4_607 0.26 +0.2 699,918 - Pf1N1B4_607 0.26 -0.8 699,918 - Pf1N1B4_607 0.26 -0.1 699,975 + Pf1N1B4_607 0.28 -0.5 699,976 - Pf1N1B4_607 0.28 +0.7 699,978 - Pf1N1B4_607 0.28 -0.6 700,011 - Pf1N1B4_607 0.30 +0.1 700,011 - Pf1N1B4_607 0.30 +0.5 700,011 - Pf1N1B4_607 0.30 +0.4 700,013 + Pf1N1B4_607 0.30 -0.1 700,013 + Pf1N1B4_607 0.30 -0.1 700,013 + Pf1N1B4_607 0.30 -1.6 700,013 + Pf1N1B4_607 0.30 -1.6 700,014 - Pf1N1B4_607 0.30 -0.1 700,014 - Pf1N1B4_607 0.30 +0.3 700,014 - Pf1N1B4_607 0.30 +1.7 700,014 - Pf1N1B4_607 0.30 -1.3 700,014 - Pf1N1B4_607 0.30 -2.9 700,079 + Pf1N1B4_607 0.32 -0.6
Or see this region's nucleotide sequence