Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5996 and Pf1N1B4_5997 are separated by 87 nucleotides Pf1N1B4_5997 and Pf1N1B4_5998 are separated by 14 nucleotides
Pf1N1B4_5996 - Transposase, at 447,126 to 448,178
_5996
Pf1N1B4_5997 - Taurine dioxygenase (EC 1.14.11.17), at 448,266 to 449,114
_5997
Pf1N1B4_5998 - Quinone oxidoreductase (EC 1.6.5.5), at 449,129 to 450,109
_5998
Position (kb) 448
449
450 Strain fitness (log2 ratio) -2
-1
0
1
2 at 447.344 kb on + strand, within Pf1N1B4_5996 at 447.345 kb on - strand, within Pf1N1B4_5996 at 447.345 kb on - strand, within Pf1N1B4_5996 at 447.375 kb on - strand, within Pf1N1B4_5996 at 447.544 kb on + strand, within Pf1N1B4_5996 at 447.544 kb on + strand, within Pf1N1B4_5996 at 447.544 kb on + strand, within Pf1N1B4_5996 at 447.545 kb on - strand, within Pf1N1B4_5996 at 447.545 kb on - strand, within Pf1N1B4_5996 at 448.027 kb on + strand, within Pf1N1B4_5996 at 448.111 kb on + strand at 448.184 kb on - strand at 448.189 kb on + strand at 448.189 kb on + strand at 448.189 kb on + strand at 448.189 kb on + strand at 448.190 kb on - strand at 448.195 kb on + strand at 448.195 kb on + strand at 448.270 kb on + strand at 448.350 kb on - strand at 448.406 kb on - strand, within Pf1N1B4_5997 at 448.417 kb on + strand, within Pf1N1B4_5997 at 448.418 kb on - strand, within Pf1N1B4_5997 at 448.418 kb on - strand, within Pf1N1B4_5997 at 448.501 kb on + strand, within Pf1N1B4_5997 at 448.501 kb on + strand, within Pf1N1B4_5997 at 448.502 kb on - strand at 448.594 kb on + strand, within Pf1N1B4_5997 at 448.868 kb on - strand, within Pf1N1B4_5997 at 449.130 kb on + strand at 449.131 kb on - strand at 449.131 kb on - strand at 449.208 kb on + strand at 449.209 kb on - strand at 449.209 kb on - strand at 449.301 kb on + strand at 449.301 kb on + strand, within Pf1N1B4_5998 at 449.301 kb on + strand, within Pf1N1B4_5998 at 449.301 kb on + strand, within Pf1N1B4_5998 at 449.302 kb on - strand, within Pf1N1B4_5998 at 449.532 kb on + strand at 449.532 kb on + strand, within Pf1N1B4_5998 at 449.533 kb on - strand, within Pf1N1B4_5998 at 449.533 kb on - strand, within Pf1N1B4_5998 at 449.533 kb on - strand, within Pf1N1B4_5998 at 449.533 kb on - strand, within Pf1N1B4_5998 at 449.718 kb on + strand, within Pf1N1B4_5998 at 449.824 kb on - strand, within Pf1N1B4_5998 at 449.836 kb on + strand, within Pf1N1B4_5998 at 449.837 kb on - strand, within Pf1N1B4_5998 at 449.837 kb on - strand, within Pf1N1B4_5998 at 449.853 kb on + strand at 449.854 kb on - strand, within Pf1N1B4_5998 at 449.876 kb on + strand, within Pf1N1B4_5998 at 449.876 kb on + strand at 449.876 kb on + strand, within Pf1N1B4_5998 at 449.876 kb on + strand, within Pf1N1B4_5998 at 449.876 kb on + strand, within Pf1N1B4_5998 at 449.876 kb on + strand at 449.877 kb on - strand, within Pf1N1B4_5998 at 449.973 kb on + strand, within Pf1N1B4_5998 at 449.973 kb on + strand, within Pf1N1B4_5998 at 449.974 kb on - strand, within Pf1N1B4_5998 at 449.974 kb on - strand, within Pf1N1B4_5998 at 449.974 kb on - strand, within Pf1N1B4_5998
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 447,344 + Pf1N1B4_5996 0.21 -1.3 447,345 - Pf1N1B4_5996 0.21 -1.9 447,345 - Pf1N1B4_5996 0.21 +0.7 447,375 - Pf1N1B4_5996 0.24 -1.1 447,544 + Pf1N1B4_5996 0.40 +1.4 447,544 + Pf1N1B4_5996 0.40 -0.3 447,544 + Pf1N1B4_5996 0.40 -0.3 447,545 - Pf1N1B4_5996 0.40 -0.3 447,545 - Pf1N1B4_5996 0.40 -0.9 448,027 + Pf1N1B4_5996 0.86 -0.7 448,111 + -0.9 448,184 - +1.7 448,189 + -0.3 448,189 + -0.6 448,189 + -1.0 448,189 + +1.3 448,190 - -1.7 448,195 + -0.3 448,195 + +1.3 448,270 + -0.9 448,350 - +2.5 448,406 - Pf1N1B4_5997 0.16 +0.1 448,417 + Pf1N1B4_5997 0.18 -1.3 448,418 - Pf1N1B4_5997 0.18 -1.3 448,418 - Pf1N1B4_5997 0.18 -0.9 448,501 + Pf1N1B4_5997 0.28 -0.3 448,501 + Pf1N1B4_5997 0.28 +0.7 448,502 - -0.3 448,594 + Pf1N1B4_5997 0.39 +0.3 448,868 - Pf1N1B4_5997 0.71 -0.0 449,130 + -1.3 449,131 - +1.0 449,131 - -1.9 449,208 + -0.3 449,209 - -0.3 449,209 - -1.9 449,301 + -0.3 449,301 + Pf1N1B4_5998 0.18 -1.9 449,301 + Pf1N1B4_5998 0.18 +0.1 449,301 + Pf1N1B4_5998 0.18 +0.3 449,302 - Pf1N1B4_5998 0.18 -0.3 449,532 + +0.7 449,532 + Pf1N1B4_5998 0.41 -0.9 449,533 - Pf1N1B4_5998 0.41 -0.3 449,533 - Pf1N1B4_5998 0.41 -0.3 449,533 - Pf1N1B4_5998 0.41 -0.1 449,533 - Pf1N1B4_5998 0.41 -0.9 449,718 + Pf1N1B4_5998 0.60 -2.3 449,824 - Pf1N1B4_5998 0.71 -2.3 449,836 + Pf1N1B4_5998 0.72 -0.3 449,837 - Pf1N1B4_5998 0.72 +2.1 449,837 - Pf1N1B4_5998 0.72 +1.7 449,853 + -1.3 449,854 - Pf1N1B4_5998 0.74 -0.6 449,876 + Pf1N1B4_5998 0.76 +0.7 449,876 + -1.9 449,876 + Pf1N1B4_5998 0.76 +0.7 449,876 + Pf1N1B4_5998 0.76 -0.8 449,876 + Pf1N1B4_5998 0.76 +1.0 449,876 + -0.3 449,877 - Pf1N1B4_5998 0.76 -0.3 449,973 + Pf1N1B4_5998 0.86 -0.3 449,973 + Pf1N1B4_5998 0.86 -1.9 449,974 - Pf1N1B4_5998 0.86 +0.4 449,974 - Pf1N1B4_5998 0.86 -0.7 449,974 - Pf1N1B4_5998 0.86 -2.3
Or see this region's nucleotide sequence