Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_590 and Pf1N1B4_591 are separated by 108 nucleotides Pf1N1B4_591 and Pf1N1B4_592 are separated by 231 nucleotides
Pf1N1B4_590 - Phosphogluconate repressor HexR, RpiR family, at 680,911 to 681,780
_590
Pf1N1B4_591 - Aldose 1-epimerase, at 681,889 to 682,758
_591
Pf1N1B4_592 - Glucose-selective porin OprB, at 682,990 to 684,336
_592
Position (kb) 681
682
683 Strain fitness (log2 ratio) -2
-1
0
1
2 at 680.898 kb on + strand at 680.899 kb on - strand at 681.160 kb on + strand, within Pf1N1B4_590 at 681.160 kb on + strand, within Pf1N1B4_590 at 681.160 kb on + strand at 681.161 kb on - strand, within Pf1N1B4_590 at 681.161 kb on - strand, within Pf1N1B4_590 at 681.161 kb on - strand, within Pf1N1B4_590 at 681.202 kb on + strand, within Pf1N1B4_590 at 681.203 kb on - strand, within Pf1N1B4_590 at 681.203 kb on - strand, within Pf1N1B4_590 at 681.204 kb on + strand, within Pf1N1B4_590 at 681.618 kb on + strand, within Pf1N1B4_590 at 681.618 kb on + strand, within Pf1N1B4_590 at 681.619 kb on - strand, within Pf1N1B4_590 at 681.619 kb on - strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.622 kb on + strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.623 kb on - strand, within Pf1N1B4_590 at 681.624 kb on + strand, within Pf1N1B4_590 at 681.624 kb on + strand, within Pf1N1B4_590 at 681.624 kb on + strand, within Pf1N1B4_590 at 681.625 kb on - strand, within Pf1N1B4_590 at 681.774 kb on + strand at 681.774 kb on + strand at 681.774 kb on + strand at 681.774 kb on + strand at 681.775 kb on - strand at 681.778 kb on + strand at 681.779 kb on - strand at 682.007 kb on - strand, within Pf1N1B4_591 at 682.007 kb on - strand at 682.269 kb on + strand, within Pf1N1B4_591 at 682.269 kb on + strand, within Pf1N1B4_591 at 682.396 kb on + strand, within Pf1N1B4_591 at 682.397 kb on - strand, within Pf1N1B4_591 at 682.397 kb on - strand, within Pf1N1B4_591 at 682.397 kb on - strand, within Pf1N1B4_591 at 682.397 kb on - strand, within Pf1N1B4_591 at 682.397 kb on - strand, within Pf1N1B4_591 at 682.742 kb on - strand at 682.843 kb on + strand at 682.843 kb on + strand at 682.843 kb on + strand at 682.843 kb on + strand at 682.843 kb on + strand at 682.965 kb on + strand at 682.965 kb on + strand at 682.965 kb on + strand at 682.965 kb on + strand at 682.966 kb on - strand at 682.966 kb on - strand at 682.966 kb on - strand at 682.966 kb on - strand at 682.966 kb on - strand at 683.066 kb on + strand at 683.066 kb on + strand at 683.066 kb on + strand at 683.066 kb on + strand at 683.066 kb on + strand at 683.067 kb on - strand at 683.067 kb on - strand at 683.067 kb on - strand at 683.067 kb on - strand at 683.067 kb on - strand at 683.067 kb on - strand at 683.067 kb on - strand at 683.067 kb on - strand at 683.067 kb on - strand at 683.067 kb on - strand at 683.067 kb on - strand at 683.067 kb on - strand at 683.067 kb on - strand at 683.067 kb on - strand at 683.067 kb on - strand at 683.067 kb on - strand at 683.067 kb on - strand at 683.067 kb on - strand at 683.076 kb on + strand at 683.115 kb on - strand at 683.115 kb on - strand at 683.115 kb on - strand at 683.115 kb on - strand at 683.127 kb on - strand, within Pf1N1B4_592 at 683.127 kb on - strand, within Pf1N1B4_592 at 683.133 kb on - strand, within Pf1N1B4_592 at 683.133 kb on - strand, within Pf1N1B4_592 at 683.133 kb on - strand, within Pf1N1B4_592 at 683.171 kb on + strand, within Pf1N1B4_592 at 683.171 kb on + strand, within Pf1N1B4_592 at 683.172 kb on - strand, within Pf1N1B4_592 at 683.172 kb on - strand, within Pf1N1B4_592 at 683.269 kb on - strand, within Pf1N1B4_592 at 683.294 kb on + strand, within Pf1N1B4_592 at 683.294 kb on + strand, within Pf1N1B4_592 at 683.295 kb on - strand at 683.295 kb on - strand, within Pf1N1B4_592 at 683.295 kb on - strand, within Pf1N1B4_592 at 683.295 kb on - strand, within Pf1N1B4_592 at 683.315 kb on + strand, within Pf1N1B4_592 at 683.315 kb on + strand, within Pf1N1B4_592 at 683.315 kb on + strand, within Pf1N1B4_592 at 683.316 kb on - strand, within Pf1N1B4_592 at 683.316 kb on - strand, within Pf1N1B4_592 at 683.316 kb on - strand, within Pf1N1B4_592 at 683.429 kb on + strand, within Pf1N1B4_592 at 683.457 kb on - strand, within Pf1N1B4_592 at 683.531 kb on + strand, within Pf1N1B4_592 at 683.531 kb on + strand, within Pf1N1B4_592 at 683.531 kb on + strand, within Pf1N1B4_592 at 683.531 kb on + strand at 683.531 kb on + strand, within Pf1N1B4_592 at 683.532 kb on - strand, within Pf1N1B4_592 at 683.532 kb on - strand, within Pf1N1B4_592 at 683.532 kb on - strand, within Pf1N1B4_592 at 683.547 kb on - strand, within Pf1N1B4_592 at 683.614 kb on - strand, within Pf1N1B4_592 at 683.672 kb on + strand, within Pf1N1B4_592 at 683.672 kb on + strand, within Pf1N1B4_592 at 683.690 kb on + strand, within Pf1N1B4_592 at 683.691 kb on - strand, within Pf1N1B4_592 at 683.691 kb on - strand, within Pf1N1B4_592 at 683.711 kb on + strand, within Pf1N1B4_592 at 683.711 kb on + strand, within Pf1N1B4_592 at 683.712 kb on - strand, within Pf1N1B4_592 at 683.712 kb on - strand, within Pf1N1B4_592 at 683.712 kb on - strand, within Pf1N1B4_592 at 683.712 kb on - strand, within Pf1N1B4_592 at 683.712 kb on - strand, within Pf1N1B4_592
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 680,898 + -1.1 680,899 - -0.1 681,160 + Pf1N1B4_590 0.29 -0.1 681,160 + Pf1N1B4_590 0.29 -0.1 681,160 + +0.9 681,161 - Pf1N1B4_590 0.29 -0.6 681,161 - Pf1N1B4_590 0.29 -0.1 681,161 - Pf1N1B4_590 0.29 -0.8 681,202 + Pf1N1B4_590 0.33 -0.3 681,203 - Pf1N1B4_590 0.34 +0.9 681,203 - Pf1N1B4_590 0.34 +0.3 681,204 + Pf1N1B4_590 0.34 -0.5 681,618 + Pf1N1B4_590 0.81 -2.4 681,618 + Pf1N1B4_590 0.81 -0.5 681,619 - Pf1N1B4_590 0.81 -0.1 681,619 - Pf1N1B4_590 0.81 -0.1 681,622 + Pf1N1B4_590 0.82 -0.8 681,622 + Pf1N1B4_590 0.82 -1.5 681,622 + Pf1N1B4_590 0.82 -0.7 681,622 + Pf1N1B4_590 0.82 +0.6 681,622 + +0.9 681,622 + Pf1N1B4_590 0.82 +0.9 681,622 + Pf1N1B4_590 0.82 -1.7 681,622 + Pf1N1B4_590 0.82 +1.0 681,622 + Pf1N1B4_590 0.82 +0.7 681,622 + Pf1N1B4_590 0.82 -0.5 681,622 + -0.1 681,622 + Pf1N1B4_590 0.82 -0.1 681,622 + Pf1N1B4_590 0.82 +0.5 681,622 + Pf1N1B4_590 0.82 -0.3 681,622 + Pf1N1B4_590 0.82 -0.7 681,622 + Pf1N1B4_590 0.82 -1.1 681,622 + Pf1N1B4_590 0.82 -0.1 681,622 + Pf1N1B4_590 0.82 +1.5 681,622 + +1.5 681,622 + Pf1N1B4_590 0.82 +0.5 681,622 + Pf1N1B4_590 0.82 -0.1 681,622 + Pf1N1B4_590 0.82 -0.8 681,622 + Pf1N1B4_590 0.82 +0.2 681,622 + Pf1N1B4_590 0.82 +0.7 681,622 + Pf1N1B4_590 0.82 +2.3 681,622 + Pf1N1B4_590 0.82 -1.0 681,623 - Pf1N1B4_590 0.82 -0.1 681,623 - Pf1N1B4_590 0.82 +0.1 681,623 - Pf1N1B4_590 0.82 +0.9 681,623 - Pf1N1B4_590 0.82 -0.6 681,623 - Pf1N1B4_590 0.82 -0.6 681,623 - Pf1N1B4_590 0.82 +0.5 681,623 - Pf1N1B4_590 0.82 -0.1 681,623 - Pf1N1B4_590 0.82 +0.2 681,623 - Pf1N1B4_590 0.82 -1.7 681,623 - Pf1N1B4_590 0.82 +0.3 681,623 - Pf1N1B4_590 0.82 -0.1 681,623 - Pf1N1B4_590 0.82 -0.1 681,623 - Pf1N1B4_590 0.82 -0.1 681,623 - Pf1N1B4_590 0.82 -1.3 681,623 - Pf1N1B4_590 0.82 -0.7 681,623 - Pf1N1B4_590 0.82 +0.8 681,623 - Pf1N1B4_590 0.82 +0.9 681,623 - Pf1N1B4_590 0.82 -0.1 681,623 - Pf1N1B4_590 0.82 -0.7 681,623 - Pf1N1B4_590 0.82 +0.2 681,623 - Pf1N1B4_590 0.82 +1.9 681,623 - Pf1N1B4_590 0.82 +0.3 681,623 - Pf1N1B4_590 0.82 -0.6 681,623 - Pf1N1B4_590 0.82 +0.9 681,623 - Pf1N1B4_590 0.82 +0.2 681,623 - Pf1N1B4_590 0.82 -0.1 681,623 - Pf1N1B4_590 0.82 -0.5 681,623 - Pf1N1B4_590 0.82 -1.1 681,623 - Pf1N1B4_590 0.82 +0.4 681,623 - Pf1N1B4_590 0.82 +0.4 681,623 - Pf1N1B4_590 0.82 -0.1 681,623 - Pf1N1B4_590 0.82 +0.3 681,623 - Pf1N1B4_590 0.82 -0.6 681,623 - Pf1N1B4_590 0.82 +0.2 681,623 - Pf1N1B4_590 0.82 -0.7 681,623 - Pf1N1B4_590 0.82 -0.7 681,623 - Pf1N1B4_590 0.82 -1.7 681,623 - Pf1N1B4_590 0.82 -1.7 681,624 + Pf1N1B4_590 0.82 -1.7 681,624 + Pf1N1B4_590 0.82 -0.1 681,624 + Pf1N1B4_590 0.82 -0.1 681,625 - Pf1N1B4_590 0.82 -0.1 681,774 + -1.5 681,774 + -1.1 681,774 + -0.1 681,774 + +2.3 681,775 - -1.1 681,778 + -0.1 681,779 - -0.7 682,007 - Pf1N1B4_591 0.14 -0.8 682,007 - -0.1 682,269 + Pf1N1B4_591 0.44 -1.7 682,269 + Pf1N1B4_591 0.44 -1.3 682,396 + Pf1N1B4_591 0.58 -2.1 682,397 - Pf1N1B4_591 0.58 -1.3 682,397 - Pf1N1B4_591 0.58 -0.7 682,397 - Pf1N1B4_591 0.58 -0.7 682,397 - Pf1N1B4_591 0.58 -1.1 682,397 - Pf1N1B4_591 0.58 -1.7 682,742 - -2.4 682,843 + +1.9 682,843 + -0.7 682,843 + -0.8 682,843 + +0.1 682,843 + -0.8 682,965 + +0.9 682,965 + -1.1 682,965 + -2.7 682,965 + -1.7 682,966 - -0.3 682,966 - +0.5 682,966 - +0.2 682,966 - -1.4 682,966 - -0.1 683,066 + +2.5 683,066 + -0.1 683,066 + -1.3 683,066 + -1.7 683,066 + +0.3 683,067 - -0.1 683,067 - -2.1 683,067 - -1.1 683,067 - -0.3 683,067 - +1.3 683,067 - +0.3 683,067 - -0.1 683,067 - +0.3 683,067 - -1.1 683,067 - -0.3 683,067 - +0.3 683,067 - +0.7 683,067 - +0.3 683,067 - +0.7 683,067 - -0.2 683,067 - -1.1 683,067 - +0.7 683,067 - -0.5 683,076 + -0.6 683,115 - -0.1 683,115 - +0.3 683,115 - +0.3 683,115 - +0.5 683,127 - Pf1N1B4_592 0.10 -0.1 683,127 - Pf1N1B4_592 0.10 +0.5 683,133 - Pf1N1B4_592 0.11 -0.1 683,133 - Pf1N1B4_592 0.11 -0.1 683,133 - Pf1N1B4_592 0.11 -0.8 683,171 + Pf1N1B4_592 0.13 +1.5 683,171 + Pf1N1B4_592 0.13 -0.5 683,172 - Pf1N1B4_592 0.14 -1.7 683,172 - Pf1N1B4_592 0.14 +0.5 683,269 - Pf1N1B4_592 0.21 +0.6 683,294 + Pf1N1B4_592 0.23 +1.9 683,294 + Pf1N1B4_592 0.23 +2.3 683,295 - -0.1 683,295 - Pf1N1B4_592 0.23 -0.1 683,295 - Pf1N1B4_592 0.23 -0.1 683,295 - Pf1N1B4_592 0.23 -0.5 683,315 + Pf1N1B4_592 0.24 +0.7 683,315 + Pf1N1B4_592 0.24 -0.1 683,315 + Pf1N1B4_592 0.24 +0.2 683,316 - Pf1N1B4_592 0.24 -0.1 683,316 - Pf1N1B4_592 0.24 -0.1 683,316 - Pf1N1B4_592 0.24 +0.7 683,429 + Pf1N1B4_592 0.33 -1.4 683,457 - Pf1N1B4_592 0.35 +0.5 683,531 + Pf1N1B4_592 0.40 +0.3 683,531 + Pf1N1B4_592 0.40 -0.1 683,531 + Pf1N1B4_592 0.40 -0.1 683,531 + -0.7 683,531 + Pf1N1B4_592 0.40 -0.3 683,532 - Pf1N1B4_592 0.40 -1.1 683,532 - Pf1N1B4_592 0.40 -1.4 683,532 - Pf1N1B4_592 0.40 +0.3 683,547 - Pf1N1B4_592 0.41 -0.5 683,614 - Pf1N1B4_592 0.46 -0.1 683,672 + Pf1N1B4_592 0.51 +1.9 683,672 + Pf1N1B4_592 0.51 -1.4 683,690 + Pf1N1B4_592 0.52 -1.7 683,691 - Pf1N1B4_592 0.52 -0.1 683,691 - Pf1N1B4_592 0.52 -1.1 683,711 + Pf1N1B4_592 0.54 -1.1 683,711 + Pf1N1B4_592 0.54 -0.1 683,712 - Pf1N1B4_592 0.54 -0.9 683,712 - Pf1N1B4_592 0.54 +0.3 683,712 - Pf1N1B4_592 0.54 -0.1 683,712 - Pf1N1B4_592 0.54 -1.1 683,712 - Pf1N1B4_592 0.54 -0.6
Or see this region's nucleotide sequence