Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5906 and Pf1N1B4_5907 are separated by 63 nucleotides Pf1N1B4_5907 and Pf1N1B4_5908 are separated by 89 nucleotides Pf1N1B4_5908 and Pf1N1B4_5909 are separated by 70 nucleotides
Pf1N1B4_5906 - Esterase/lipase, at 348,829 to 349,845
_5906
Pf1N1B4_5907 - cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases, at 349,909 to 351,336
_5907
Pf1N1B4_5908 - Nicotinamidase family protein YcaC, at 351,426 to 352,052
_5908
Pf1N1B4_5909 - Exoenzymes regulatory protein AepA precursor, at 352,123 to 353,961
_5909
Position (kb) 349
350
351
352 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 349.234 kb on + strand, within Pf1N1B4_5906 at 349.235 kb on - strand, within Pf1N1B4_5906 at 349.292 kb on - strand, within Pf1N1B4_5906 at 349.553 kb on - strand, within Pf1N1B4_5906 at 349.688 kb on - strand, within Pf1N1B4_5906 at 349.736 kb on - strand, within Pf1N1B4_5906 at 349.805 kb on + strand at 349.844 kb on - strand at 349.844 kb on - strand at 349.844 kb on - strand at 350.138 kb on + strand, within Pf1N1B4_5907 at 350.138 kb on + strand, within Pf1N1B4_5907 at 350.204 kb on + strand, within Pf1N1B4_5907 at 350.204 kb on + strand, within Pf1N1B4_5907 at 350.204 kb on + strand, within Pf1N1B4_5907 at 350.205 kb on - strand, within Pf1N1B4_5907 at 350.205 kb on - strand, within Pf1N1B4_5907 at 350.205 kb on - strand, within Pf1N1B4_5907 at 350.281 kb on + strand, within Pf1N1B4_5907 at 350.361 kb on + strand, within Pf1N1B4_5907 at 350.361 kb on + strand, within Pf1N1B4_5907 at 350.728 kb on + strand, within Pf1N1B4_5907 at 350.956 kb on + strand, within Pf1N1B4_5907 at 350.956 kb on + strand at 351.166 kb on + strand, within Pf1N1B4_5907 at 351.167 kb on - strand, within Pf1N1B4_5907 at 351.367 kb on + strand at 351.367 kb on + strand at 351.367 kb on + strand at 351.391 kb on + strand at 351.391 kb on + strand at 351.469 kb on + strand at 351.709 kb on - strand, within Pf1N1B4_5908 at 352.005 kb on + strand at 352.037 kb on - strand at 352.037 kb on - strand at 352.079 kb on - strand at 352.079 kb on - strand at 352.124 kb on + strand at 352.124 kb on + strand at 352.124 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 349,234 + Pf1N1B4_5906 0.40 -1.7 349,235 - Pf1N1B4_5906 0.40 -1.3 349,292 - Pf1N1B4_5906 0.46 -1.3 349,553 - Pf1N1B4_5906 0.71 -0.7 349,688 - Pf1N1B4_5906 0.84 -2.7 349,736 - Pf1N1B4_5906 0.89 -1.8 349,805 + -0.9 349,844 - -0.6 349,844 - -0.3 349,844 - +2.0 350,138 + Pf1N1B4_5907 0.16 -0.5 350,138 + Pf1N1B4_5907 0.16 -0.3 350,204 + Pf1N1B4_5907 0.21 -0.3 350,204 + Pf1N1B4_5907 0.21 -0.7 350,204 + Pf1N1B4_5907 0.21 +1.3 350,205 - Pf1N1B4_5907 0.21 -1.2 350,205 - Pf1N1B4_5907 0.21 -2.9 350,205 - Pf1N1B4_5907 0.21 -0.3 350,281 + Pf1N1B4_5907 0.26 -0.3 350,361 + Pf1N1B4_5907 0.32 +0.7 350,361 + Pf1N1B4_5907 0.32 +0.7 350,728 + Pf1N1B4_5907 0.57 -1.1 350,956 + Pf1N1B4_5907 0.73 -0.2 350,956 + +0.7 351,166 + Pf1N1B4_5907 0.88 +0.4 351,167 - Pf1N1B4_5907 0.88 +2.0 351,367 + -2.3 351,367 + -0.9 351,367 + -0.7 351,391 + -0.3 351,391 + -1.3 351,469 + -0.4 351,709 - Pf1N1B4_5908 0.45 -2.7 352,005 + -1.7 352,037 - -1.7 352,037 - -1.1 352,079 - +0.3 352,079 - -1.1 352,124 + -0.3 352,124 + -0.9 352,124 + +1.7
Or see this region's nucleotide sequence