Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5901 and Pf1N1B4_5902 are separated by 305 nucleotides Pf1N1B4_5902 and Pf1N1B4_5903 are separated by 2 nucleotides Pf1N1B4_5903 and Pf1N1B4_5904 overlap by 12 nucleotides Pf1N1B4_5904 and Pf1N1B4_5905 are separated by 24 nucleotides
Pf1N1B4_5901 - hypothetical protein, at 345,393 to 346,082
_5901
Pf1N1B4_5902 - FIG022160: hypothetical toxin, at 346,388 to 346,528
_5902
Pf1N1B4_5903 - FIG045511: hypothetical antitoxin (to FIG022160: hypothetical toxin), at 346,531 to 346,839
_5903
Pf1N1B4_5904 - FIG00955320: hypothetical protein, at 346,828 to 347,736
_5904
Pf1N1B4_5905 - Transcriptional regulator, LysR family, at 347,761 to 348,666
_5905
Position (kb) 346
347
348 Strain fitness (log2 ratio) -2
-1
0
1 at 345.846 kb on - strand, within Pf1N1B4_5901 at 345.943 kb on - strand, within Pf1N1B4_5901 at 345.972 kb on + strand, within Pf1N1B4_5901 at 345.973 kb on - strand, within Pf1N1B4_5901 at 346.015 kb on - strand at 346.181 kb on - strand at 346.181 kb on - strand at 346.223 kb on + strand at 346.224 kb on - strand at 346.224 kb on - strand at 346.224 kb on - strand at 346.242 kb on - strand at 346.247 kb on - strand at 346.432 kb on - strand, within Pf1N1B4_5902 at 346.527 kb on - strand at 346.598 kb on - strand at 346.767 kb on - strand, within Pf1N1B4_5903 at 346.856 kb on - strand at 346.886 kb on + strand at 346.928 kb on + strand, within Pf1N1B4_5904 at 346.929 kb on - strand, within Pf1N1B4_5904 at 347.034 kb on - strand, within Pf1N1B4_5904 at 347.180 kb on + strand, within Pf1N1B4_5904 at 347.957 kb on + strand, within Pf1N1B4_5905 at 348.115 kb on + strand, within Pf1N1B4_5905 at 348.116 kb on - strand, within Pf1N1B4_5905 at 348.155 kb on + strand, within Pf1N1B4_5905 at 348.155 kb on + strand, within Pf1N1B4_5905 at 348.155 kb on + strand, within Pf1N1B4_5905 at 348.251 kb on + strand, within Pf1N1B4_5905 at 348.473 kb on + strand, within Pf1N1B4_5905 at 348.473 kb on + strand at 348.474 kb on - strand, within Pf1N1B4_5905 at 348.589 kb on + strand at 348.589 kb on + strand at 348.590 kb on - strand at 348.642 kb on + strand at 348.643 kb on - strand at 348.643 kb on - strand at 348.643 kb on - strand at 348.648 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 345,846 - Pf1N1B4_5901 0.66 -0.3 345,943 - Pf1N1B4_5901 0.80 +0.4 345,972 + Pf1N1B4_5901 0.84 -0.3 345,973 - Pf1N1B4_5901 0.84 -2.3 346,015 - -0.3 346,181 - -0.3 346,181 - +0.3 346,223 + -2.3 346,224 - -0.3 346,224 - +1.3 346,224 - -1.1 346,242 - -1.6 346,247 - -0.3 346,432 - Pf1N1B4_5902 0.31 -0.9 346,527 - -1.1 346,598 - -1.9 346,767 - Pf1N1B4_5903 0.76 -1.3 346,856 - -0.1 346,886 + -0.3 346,928 + Pf1N1B4_5904 0.11 -0.7 346,929 - Pf1N1B4_5904 0.11 +0.1 347,034 - Pf1N1B4_5904 0.23 -0.8 347,180 + Pf1N1B4_5904 0.39 -1.3 347,957 + Pf1N1B4_5905 0.22 -0.4 348,115 + Pf1N1B4_5905 0.39 -1.1 348,116 - Pf1N1B4_5905 0.39 -0.4 348,155 + Pf1N1B4_5905 0.43 -0.6 348,155 + Pf1N1B4_5905 0.43 -2.3 348,155 + Pf1N1B4_5905 0.43 +0.7 348,251 + Pf1N1B4_5905 0.54 -0.3 348,473 + Pf1N1B4_5905 0.79 -0.3 348,473 + -0.7 348,474 - Pf1N1B4_5905 0.79 +0.7 348,589 + -0.3 348,589 + -1.3 348,590 - -0.3 348,642 + -0.3 348,643 - +1.3 348,643 - -0.3 348,643 - -1.9 348,648 + -1.3
Or see this region's nucleotide sequence