Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5834

Experiment: L-Isoleucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5832 and Pf1N1B4_5833 are separated by 243 nucleotidesPf1N1B4_5833 and Pf1N1B4_5834 are separated by 36 nucleotidesPf1N1B4_5834 and Pf1N1B4_5835 are separated by 11 nucleotides Pf1N1B4_5832 - LysR family transcriptional regulator near succinyl-CoA:3-ketoacid-coenzyme A transferase, at 266,702 to 267,601 _5832 Pf1N1B4_5833 - Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A (EC 2.8.3.5), at 267,845 to 268,543 _5833 Pf1N1B4_5834 - Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B (EC 2.8.3.5), at 268,580 to 269,245 _5834 Pf1N1B4_5835 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 269,257 to 270,438 _5835 Position (kb) 268 269 270Strain fitness (log2 ratio) -2 -1 0 1at 267.831 kb on + strandat 267.832 kb on - strandat 267.832 kb on - strandat 267.832 kb on - strandat 267.832 kb on - strandat 267.844 kb on + strandat 267.845 kb on - strandat 267.845 kb on - strandat 267.870 kb on - strandat 268.083 kb on - strand, within Pf1N1B4_5833at 268.275 kb on - strand, within Pf1N1B4_5833at 268.478 kb on + strandat 268.478 kb on + strandat 268.478 kb on + strandat 268.478 kb on + strandat 268.479 kb on - strandat 268.479 kb on - strandat 268.572 kb on + strandat 268.640 kb on + strandat 268.640 kb on + strandat 268.640 kb on + strandat 268.641 kb on - strandat 268.682 kb on + strand, within Pf1N1B4_5834at 268.682 kb on + strand, within Pf1N1B4_5834at 268.683 kb on - strand, within Pf1N1B4_5834at 268.683 kb on - strand, within Pf1N1B4_5834at 268.683 kb on - strand, within Pf1N1B4_5834at 269.423 kb on + strand, within Pf1N1B4_5835at 269.623 kb on + strand, within Pf1N1B4_5835at 269.624 kb on - strand, within Pf1N1B4_5835at 269.624 kb on - strand, within Pf1N1B4_5835at 269.624 kb on - strand, within Pf1N1B4_5835at 269.758 kb on + strand, within Pf1N1B4_5835at 269.759 kb on - strand, within Pf1N1B4_5835at 269.759 kb on - strand, within Pf1N1B4_5835

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (N)
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267,831 + +1.7
267,832 - -1.3
267,832 - +1.3
267,832 - -0.3
267,832 - -1.7
267,844 + -0.9
267,845 - -0.7
267,845 - -0.9
267,870 - -0.7
268,083 - Pf1N1B4_5833 0.34 +0.2
268,275 - Pf1N1B4_5833 0.62 +0.3
268,478 + -0.3
268,478 + +1.3
268,478 + +0.2
268,478 + -1.3
268,479 - -0.3
268,479 - -0.3
268,572 + -1.1
268,640 + +0.7
268,640 + +1.7
268,640 + -1.7
268,641 - -1.9
268,682 + Pf1N1B4_5834 0.15 -2.3
268,682 + Pf1N1B4_5834 0.15 -0.0
268,683 - Pf1N1B4_5834 0.15 +0.7
268,683 - Pf1N1B4_5834 0.15 -2.3
268,683 - Pf1N1B4_5834 0.15 -1.6
269,423 + Pf1N1B4_5835 0.14 -0.6
269,623 + Pf1N1B4_5835 0.31 -2.3
269,624 - Pf1N1B4_5835 0.31 -0.3
269,624 - Pf1N1B4_5835 0.31 -0.2
269,624 - Pf1N1B4_5835 0.31 +0.2
269,758 + Pf1N1B4_5835 0.42 -1.3
269,759 - Pf1N1B4_5835 0.42 +0.7
269,759 - Pf1N1B4_5835 0.42 +1.3

Or see this region's nucleotide sequence