Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5522

Experiment: L-Isoleucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5521 and Pf1N1B4_5522 are separated by 9 nucleotidesPf1N1B4_5522 and Pf1N1B4_5523 are separated by 96 nucleotides Pf1N1B4_5521 - General secretion pathway protein D / Type II secretion outermembrane pore forming protein (PulD), at 278,116 to 279,762 _5521 Pf1N1B4_5522 - hypothetical protein, at 279,772 to 280,713 _5522 Pf1N1B4_5523 - hypothetical protein, at 280,810 to 282,381 _5523 Position (kb) 279 280 281Strain fitness (log2 ratio) -2 -1 0 1 2at 278.794 kb on + strand, within Pf1N1B4_5521at 278.794 kb on + strand, within Pf1N1B4_5521at 278.849 kb on + strand, within Pf1N1B4_5521at 278.850 kb on - strand, within Pf1N1B4_5521at 278.850 kb on - strand, within Pf1N1B4_5521at 278.862 kb on + strand, within Pf1N1B4_5521at 278.862 kb on + strand, within Pf1N1B4_5521at 278.907 kb on - strand, within Pf1N1B4_5521at 278.917 kb on + strand, within Pf1N1B4_5521at 278.981 kb on + strandat 278.981 kb on + strand, within Pf1N1B4_5521at 278.981 kb on + strand, within Pf1N1B4_5521at 278.981 kb on + strand, within Pf1N1B4_5521at 278.981 kb on + strand, within Pf1N1B4_5521at 278.982 kb on - strand, within Pf1N1B4_5521at 278.982 kb on - strand, within Pf1N1B4_5521at 278.982 kb on - strand, within Pf1N1B4_5521at 278.982 kb on - strand, within Pf1N1B4_5521at 278.982 kb on - strand, within Pf1N1B4_5521at 279.048 kb on + strand, within Pf1N1B4_5521at 279.049 kb on - strand, within Pf1N1B4_5521at 279.049 kb on - strand, within Pf1N1B4_5521at 279.264 kb on + strand, within Pf1N1B4_5521at 279.282 kb on + strand, within Pf1N1B4_5521at 279.282 kb on + strand, within Pf1N1B4_5521at 279.282 kb on + strand, within Pf1N1B4_5521at 279.282 kb on + strand, within Pf1N1B4_5521at 279.283 kb on - strand, within Pf1N1B4_5521at 279.371 kb on + strand, within Pf1N1B4_5521at 279.371 kb on + strand, within Pf1N1B4_5521at 279.371 kb on + strand, within Pf1N1B4_5521at 279.372 kb on - strandat 279.372 kb on - strand, within Pf1N1B4_5521at 279.423 kb on - strand, within Pf1N1B4_5521at 279.523 kb on - strand, within Pf1N1B4_5521at 279.532 kb on + strand, within Pf1N1B4_5521at 279.532 kb on + strand, within Pf1N1B4_5521at 279.532 kb on + strand, within Pf1N1B4_5521at 279.533 kb on - strand, within Pf1N1B4_5521at 279.533 kb on - strand, within Pf1N1B4_5521at 279.566 kb on + strand, within Pf1N1B4_5521at 279.567 kb on - strand, within Pf1N1B4_5521at 279.570 kb on + strand, within Pf1N1B4_5521at 279.570 kb on + strand, within Pf1N1B4_5521at 279.571 kb on - strand, within Pf1N1B4_5521at 279.571 kb on - strand, within Pf1N1B4_5521at 279.571 kb on - strand, within Pf1N1B4_5521at 279.601 kb on + strandat 279.602 kb on - strandat 279.676 kb on - strandat 279.676 kb on - strandat 279.699 kb on + strandat 279.700 kb on - strandat 279.700 kb on - strandat 279.700 kb on - strandat 279.763 kb on + strandat 279.773 kb on + strandat 279.774 kb on - strandat 279.774 kb on - strandat 279.774 kb on - strandat 279.845 kb on + strandat 279.846 kb on - strandat 279.846 kb on - strandat 279.884 kb on - strand, within Pf1N1B4_5522at 280.065 kb on + strand, within Pf1N1B4_5522at 280.144 kb on + strand, within Pf1N1B4_5522at 280.144 kb on + strand, within Pf1N1B4_5522at 280.144 kb on + strand, within Pf1N1B4_5522at 280.144 kb on + strand, within Pf1N1B4_5522at 280.145 kb on - strand, within Pf1N1B4_5522at 280.145 kb on - strand, within Pf1N1B4_5522at 280.145 kb on - strand, within Pf1N1B4_5522at 280.145 kb on - strand, within Pf1N1B4_5522at 280.145 kb on - strand, within Pf1N1B4_5522at 280.522 kb on + strand, within Pf1N1B4_5522at 280.523 kb on - strand, within Pf1N1B4_5522at 280.523 kb on - strand, within Pf1N1B4_5522at 280.523 kb on - strand, within Pf1N1B4_5522at 280.598 kb on + strand, within Pf1N1B4_5522at 280.599 kb on - strand, within Pf1N1B4_5522at 280.599 kb on - strandat 280.599 kb on - strand, within Pf1N1B4_5522at 280.652 kb on + strandat 280.653 kb on - strandat 280.653 kb on - strandat 280.697 kb on - strandat 280.748 kb on - strandat 280.748 kb on - strandat 280.748 kb on - strandat 280.748 kb on - strandat 280.748 kb on - strandat 280.748 kb on - strandat 280.748 kb on - strandat 280.748 kb on - strandat 280.869 kb on + strandat 280.870 kb on - strandat 280.870 kb on - strandat 280.936 kb on + strandat 280.936 kb on + strandat 280.936 kb on + strandat 280.937 kb on - strandat 280.966 kb on + strandat 280.998 kb on + strand, within Pf1N1B4_5523at 280.998 kb on + strand, within Pf1N1B4_5523at 281.045 kb on - strand, within Pf1N1B4_5523at 281.057 kb on + strand, within Pf1N1B4_5523at 281.058 kb on - strand, within Pf1N1B4_5523at 281.058 kb on - strand, within Pf1N1B4_5523at 281.058 kb on - strand, within Pf1N1B4_5523at 281.122 kb on + strand, within Pf1N1B4_5523at 281.122 kb on + strand, within Pf1N1B4_5523at 281.123 kb on - strand, within Pf1N1B4_5523at 281.123 kb on - strand, within Pf1N1B4_5523at 281.123 kb on - strand, within Pf1N1B4_5523at 281.150 kb on + strand, within Pf1N1B4_5523at 281.151 kb on - strand, within Pf1N1B4_5523at 281.170 kb on - strandat 281.207 kb on - strand, within Pf1N1B4_5523at 281.207 kb on - strand, within Pf1N1B4_5523at 281.350 kb on - strand, within Pf1N1B4_5523at 281.366 kb on + strand, within Pf1N1B4_5523at 281.366 kb on + strand, within Pf1N1B4_5523at 281.366 kb on + strand, within Pf1N1B4_5523at 281.367 kb on - strand, within Pf1N1B4_5523at 281.374 kb on + strand, within Pf1N1B4_5523at 281.386 kb on + strand, within Pf1N1B4_5523at 281.386 kb on + strand, within Pf1N1B4_5523at 281.386 kb on + strand, within Pf1N1B4_5523at 281.387 kb on - strand, within Pf1N1B4_5523at 281.387 kb on - strand, within Pf1N1B4_5523at 281.387 kb on - strand, within Pf1N1B4_5523at 281.394 kb on + strand, within Pf1N1B4_5523at 281.394 kb on + strand, within Pf1N1B4_5523at 281.507 kb on - strand, within Pf1N1B4_5523at 281.528 kb on + strandat 281.547 kb on + strand, within Pf1N1B4_5523at 281.548 kb on - strand, within Pf1N1B4_5523at 281.571 kb on + strand, within Pf1N1B4_5523at 281.571 kb on + strand, within Pf1N1B4_5523at 281.585 kb on - strand, within Pf1N1B4_5523at 281.642 kb on - strand, within Pf1N1B4_5523at 281.642 kb on - strand, within Pf1N1B4_5523at 281.650 kb on + strand, within Pf1N1B4_5523at 281.651 kb on - strand, within Pf1N1B4_5523at 281.651 kb on - strand, within Pf1N1B4_5523at 281.651 kb on - strand, within Pf1N1B4_5523at 281.651 kb on - strand, within Pf1N1B4_5523at 281.651 kb on - strand, within Pf1N1B4_5523at 281.651 kb on - strand, within Pf1N1B4_5523at 281.651 kb on - strand, within Pf1N1B4_5523

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (N)
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278,794 + Pf1N1B4_5521 0.41 -0.4
278,794 + Pf1N1B4_5521 0.41 +0.0
278,849 + Pf1N1B4_5521 0.45 +1.9
278,850 - Pf1N1B4_5521 0.45 +0.5
278,850 - Pf1N1B4_5521 0.45 -0.5
278,862 + Pf1N1B4_5521 0.45 -0.3
278,862 + Pf1N1B4_5521 0.45 +0.0
278,907 - Pf1N1B4_5521 0.48 -2.0
278,917 + Pf1N1B4_5521 0.49 -0.8
278,981 + +0.0
278,981 + Pf1N1B4_5521 0.53 -1.1
278,981 + Pf1N1B4_5521 0.53 -0.7
278,981 + Pf1N1B4_5521 0.53 -2.1
278,981 + Pf1N1B4_5521 0.53 +1.0
278,982 - Pf1N1B4_5521 0.53 -1.0
278,982 - Pf1N1B4_5521 0.53 +0.8
278,982 - Pf1N1B4_5521 0.53 +1.0
278,982 - Pf1N1B4_5521 0.53 -0.7
278,982 - Pf1N1B4_5521 0.53 -0.5
279,048 + Pf1N1B4_5521 0.57 -0.5
279,049 - Pf1N1B4_5521 0.57 +1.9
279,049 - Pf1N1B4_5521 0.57 -1.0
279,264 + Pf1N1B4_5521 0.70 +0.8
279,282 + Pf1N1B4_5521 0.71 +1.6
279,282 + Pf1N1B4_5521 0.71 +0.8
279,282 + Pf1N1B4_5521 0.71 +0.0
279,282 + Pf1N1B4_5521 0.71 +1.0
279,283 - Pf1N1B4_5521 0.71 +0.0
279,371 + Pf1N1B4_5521 0.76 -2.0
279,371 + Pf1N1B4_5521 0.76 -0.5
279,371 + Pf1N1B4_5521 0.76 -0.3
279,372 - -2.0
279,372 - Pf1N1B4_5521 0.76 +2.4
279,423 - Pf1N1B4_5521 0.79 +2.0
279,523 - Pf1N1B4_5521 0.85 -0.4
279,532 + Pf1N1B4_5521 0.86 +0.0
279,532 + Pf1N1B4_5521 0.86 -2.5
279,532 + Pf1N1B4_5521 0.86 +1.2
279,533 - Pf1N1B4_5521 0.86 -0.1
279,533 - Pf1N1B4_5521 0.86 -1.0
279,566 + Pf1N1B4_5521 0.88 +1.0
279,567 - Pf1N1B4_5521 0.88 +0.4
279,570 + Pf1N1B4_5521 0.88 -0.1
279,570 + Pf1N1B4_5521 0.88 +0.0
279,571 - Pf1N1B4_5521 0.88 +0.4
279,571 - Pf1N1B4_5521 0.88 +0.4
279,571 - Pf1N1B4_5521 0.88 -1.0
279,601 + +0.6
279,602 - -0.2
279,676 - +0.2
279,676 - -0.4
279,699 + +0.6
279,700 - +1.0
279,700 - +0.6
279,700 - -0.2
279,763 + +0.9
279,773 + +1.6
279,774 - -2.3
279,774 - -1.0
279,774 - -1.5
279,845 + +0.3
279,846 - +1.5
279,846 - -0.4
279,884 - Pf1N1B4_5522 0.12 +0.4
280,065 + Pf1N1B4_5522 0.31 -0.8
280,144 + Pf1N1B4_5522 0.39 +1.0
280,144 + Pf1N1B4_5522 0.39 +0.0
280,144 + Pf1N1B4_5522 0.39 -1.5
280,144 + Pf1N1B4_5522 0.39 +1.0
280,145 - Pf1N1B4_5522 0.40 +0.9
280,145 - Pf1N1B4_5522 0.40 -1.0
280,145 - Pf1N1B4_5522 0.40 -1.0
280,145 - Pf1N1B4_5522 0.40 -1.0
280,145 - Pf1N1B4_5522 0.40 -0.4
280,522 + Pf1N1B4_5522 0.80 -1.0
280,523 - Pf1N1B4_5522 0.80 +2.6
280,523 - Pf1N1B4_5522 0.80 +0.9
280,523 - Pf1N1B4_5522 0.80 -0.5
280,598 + Pf1N1B4_5522 0.88 -1.0
280,599 - Pf1N1B4_5522 0.88 -0.3
280,599 - +0.0
280,599 - Pf1N1B4_5522 0.88 -1.4
280,652 + +0.5
280,653 - -0.8
280,653 - +0.0
280,697 - +0.3
280,748 - -2.0
280,748 - +0.0
280,748 - +0.6
280,748 - +1.6
280,748 - -0.3
280,748 - +0.5
280,748 - +0.4
280,748 - -1.4
280,869 + -0.5
280,870 - -2.7
280,870 - -0.5
280,936 + +1.0
280,936 + -0.1
280,936 + -1.1
280,937 - +1.6
280,966 + +0.5
280,998 + Pf1N1B4_5523 0.12 -0.5
280,998 + Pf1N1B4_5523 0.12 +0.2
281,045 - Pf1N1B4_5523 0.15 -1.0
281,057 + Pf1N1B4_5523 0.16 -0.1
281,058 - Pf1N1B4_5523 0.16 +0.0
281,058 - Pf1N1B4_5523 0.16 -2.3
281,058 - Pf1N1B4_5523 0.16 +0.0
281,122 + Pf1N1B4_5523 0.20 -1.4
281,122 + Pf1N1B4_5523 0.20 +2.4
281,123 - Pf1N1B4_5523 0.20 +1.0
281,123 - Pf1N1B4_5523 0.20 +1.0
281,123 - Pf1N1B4_5523 0.20 -1.5
281,150 + Pf1N1B4_5523 0.22 +1.0
281,151 - Pf1N1B4_5523 0.22 +0.0
281,170 - +0.0
281,207 - Pf1N1B4_5523 0.25 -1.0
281,207 - Pf1N1B4_5523 0.25 +0.0
281,350 - Pf1N1B4_5523 0.34 -0.8
281,366 + Pf1N1B4_5523 0.35 +1.0
281,366 + Pf1N1B4_5523 0.35 +0.0
281,366 + Pf1N1B4_5523 0.35 +0.4
281,367 - Pf1N1B4_5523 0.35 +1.0
281,374 + Pf1N1B4_5523 0.36 +1.0
281,386 + Pf1N1B4_5523 0.37 -0.3
281,386 + Pf1N1B4_5523 0.37 +0.5
281,386 + Pf1N1B4_5523 0.37 +0.0
281,387 - Pf1N1B4_5523 0.37 -0.7
281,387 - Pf1N1B4_5523 0.37 +0.8
281,387 - Pf1N1B4_5523 0.37 -2.0
281,394 + Pf1N1B4_5523 0.37 -0.7
281,394 + Pf1N1B4_5523 0.37 -1.5
281,507 - Pf1N1B4_5523 0.44 -0.4
281,528 + +0.0
281,547 + Pf1N1B4_5523 0.47 +0.0
281,548 - Pf1N1B4_5523 0.47 +1.0
281,571 + Pf1N1B4_5523 0.48 +0.0
281,571 + Pf1N1B4_5523 0.48 +0.0
281,585 - Pf1N1B4_5523 0.49 +0.0
281,642 - Pf1N1B4_5523 0.53 +0.0
281,642 - Pf1N1B4_5523 0.53 +1.4
281,650 + Pf1N1B4_5523 0.53 -0.7
281,651 - Pf1N1B4_5523 0.53 +0.8
281,651 - Pf1N1B4_5523 0.53 +1.0
281,651 - Pf1N1B4_5523 0.53 -0.1
281,651 - Pf1N1B4_5523 0.53 +0.0
281,651 - Pf1N1B4_5523 0.53 -0.4
281,651 - Pf1N1B4_5523 0.53 -0.8
281,651 - Pf1N1B4_5523 0.53 -0.3

Or see this region's nucleotide sequence