Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4970 and Pf1N1B4_4971 are separated by 77 nucleotides Pf1N1B4_4971 and Pf1N1B4_4972 overlap by 4 nucleotides Pf1N1B4_4972 and Pf1N1B4_4973 are separated by 114 nucleotides Pf1N1B4_4973 and Pf1N1B4_4974 are separated by 32 nucleotides
Pf1N1B4_4970 - hypothetical protein, at 5,511,311 to 5,511,718
_4970
Pf1N1B4_4971 - hypothetical protein, at 5,511,796 to 5,512,161
_4971
Pf1N1B4_4972 - CELL PROCESSES; Adaptation; adaptations, atypical conditions, at 5,512,158 to 5,512,988
_4972
Pf1N1B4_4973 - hypothetical protein, at 5,513,103 to 5,513,387
_4973
Pf1N1B4_4974 - Protease subunit of ATP-dependent Clp proteases, at 5,513,420 to 5,513,977
_4974
Position (kb) 5512
5513 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5511.301 kb on + strand at 5511.301 kb on + strand at 5511.302 kb on - strand at 5511.321 kb on - strand at 5511.321 kb on - strand at 5511.579 kb on - strand, within Pf1N1B4_4970 at 5511.839 kb on + strand, within Pf1N1B4_4971 at 5511.976 kb on + strand, within Pf1N1B4_4971 at 5511.977 kb on - strand, within Pf1N1B4_4971 at 5512.244 kb on + strand, within Pf1N1B4_4972 at 5512.245 kb on - strand, within Pf1N1B4_4972 at 5512.857 kb on + strand, within Pf1N1B4_4972 at 5512.857 kb on + strand, within Pf1N1B4_4972 at 5512.857 kb on + strand, within Pf1N1B4_4972 at 5512.993 kb on + strand at 5513.057 kb on - strand at 5513.095 kb on + strand at 5513.096 kb on - strand at 5513.167 kb on + strand, within Pf1N1B4_4973 at 5513.202 kb on + strand, within Pf1N1B4_4973 at 5513.203 kb on - strand, within Pf1N1B4_4973 at 5513.203 kb on - strand, within Pf1N1B4_4973 at 5513.205 kb on + strand, within Pf1N1B4_4973 at 5513.205 kb on + strand, within Pf1N1B4_4973 at 5513.258 kb on + strand, within Pf1N1B4_4973 at 5513.263 kb on - strand, within Pf1N1B4_4973 at 5513.263 kb on - strand, within Pf1N1B4_4973 at 5513.263 kb on - strand, within Pf1N1B4_4973 at 5513.263 kb on - strand, within Pf1N1B4_4973 at 5513.269 kb on + strand, within Pf1N1B4_4973 at 5513.270 kb on - strand, within Pf1N1B4_4973 at 5513.499 kb on - strand, within Pf1N1B4_4974 at 5513.634 kb on + strand, within Pf1N1B4_4974 at 5513.676 kb on + strand at 5513.821 kb on - strand, within Pf1N1B4_4974
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 5,511,301 + +0.3 5,511,301 + -0.5 5,511,302 - -0.5 5,511,321 - +0.1 5,511,321 - -0.2 5,511,579 - Pf1N1B4_4970 0.66 -0.1 5,511,839 + Pf1N1B4_4971 0.12 -0.9 5,511,976 + Pf1N1B4_4971 0.49 -0.1 5,511,977 - Pf1N1B4_4971 0.49 -0.5 5,512,244 + Pf1N1B4_4972 0.10 +1.1 5,512,245 - Pf1N1B4_4972 0.10 -2.1 5,512,857 + Pf1N1B4_4972 0.84 -1.3 5,512,857 + Pf1N1B4_4972 0.84 -1.5 5,512,857 + Pf1N1B4_4972 0.84 -0.5 5,512,993 + +0.1 5,513,057 - -0.5 5,513,095 + -0.1 5,513,096 - -0.8 5,513,167 + Pf1N1B4_4973 0.22 -0.1 5,513,202 + Pf1N1B4_4973 0.35 -0.5 5,513,203 - Pf1N1B4_4973 0.35 +0.0 5,513,203 - Pf1N1B4_4973 0.35 -0.1 5,513,205 + Pf1N1B4_4973 0.36 -1.5 5,513,205 + Pf1N1B4_4973 0.36 -3.1 5,513,258 + Pf1N1B4_4973 0.54 -1.1 5,513,263 - Pf1N1B4_4973 0.56 -0.3 5,513,263 - Pf1N1B4_4973 0.56 -2.1 5,513,263 - Pf1N1B4_4973 0.56 -1.1 5,513,263 - Pf1N1B4_4973 0.56 +1.1 5,513,269 + Pf1N1B4_4973 0.58 +0.5 5,513,270 - Pf1N1B4_4973 0.59 +0.1 5,513,499 - Pf1N1B4_4974 0.14 +0.5 5,513,634 + Pf1N1B4_4974 0.38 -0.5 5,513,676 + +0.1 5,513,821 - Pf1N1B4_4974 0.72 -0.8
Or see this region's nucleotide sequence