Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4763 and Pf1N1B4_4764 are separated by 21 nucleotides Pf1N1B4_4764 and Pf1N1B4_4765 are separated by 61 nucleotides
Pf1N1B4_4763 - 2-ketogluconate 6-phosphate reductase (EC 1.1.1.43), at 5,259,196 to 5,260,221
_4763
Pf1N1B4_4764 - 2-ketogluconate transporter, at 5,260,243 to 5,261,529
_4764
Pf1N1B4_4765 - 2-ketogluconate kinase (EC 2.7.1.13), at 5,261,591 to 5,262,610
_4765
Position (kb) 5260
5261
5262 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5259.313 kb on - strand, within Pf1N1B4_4763 at 5259.702 kb on - strand, within Pf1N1B4_4763 at 5259.793 kb on + strand, within Pf1N1B4_4763 at 5259.793 kb on + strand, within Pf1N1B4_4763 at 5259.793 kb on + strand, within Pf1N1B4_4763 at 5259.793 kb on + strand, within Pf1N1B4_4763 at 5259.793 kb on + strand at 5259.794 kb on - strand, within Pf1N1B4_4763 at 5259.915 kb on + strand, within Pf1N1B4_4763 at 5259.983 kb on + strand, within Pf1N1B4_4763 at 5259.984 kb on - strand at 5260.199 kb on + strand at 5260.199 kb on + strand at 5260.200 kb on - strand at 5260.235 kb on - strand at 5260.235 kb on - strand at 5260.352 kb on + strand at 5260.353 kb on - strand at 5260.353 kb on - strand at 5260.353 kb on - strand at 5260.401 kb on - strand, within Pf1N1B4_4764 at 5260.401 kb on - strand at 5260.517 kb on + strand, within Pf1N1B4_4764 at 5260.517 kb on + strand, within Pf1N1B4_4764 at 5260.518 kb on - strand, within Pf1N1B4_4764 at 5260.518 kb on - strand, within Pf1N1B4_4764 at 5260.550 kb on + strand, within Pf1N1B4_4764 at 5260.550 kb on + strand, within Pf1N1B4_4764 at 5260.593 kb on - strand at 5260.593 kb on - strand, within Pf1N1B4_4764 at 5260.700 kb on + strand, within Pf1N1B4_4764 at 5260.981 kb on - strand, within Pf1N1B4_4764 at 5261.315 kb on + strand at 5261.315 kb on + strand at 5261.315 kb on + strand, within Pf1N1B4_4764 at 5261.316 kb on - strand, within Pf1N1B4_4764 at 5261.435 kb on + strand at 5261.436 kb on - strand at 5261.465 kb on + strand at 5261.493 kb on - strand at 5261.493 kb on - strand at 5261.493 kb on - strand at 5261.493 kb on - strand at 5261.493 kb on - strand at 5261.493 kb on - strand at 5261.493 kb on - strand at 5261.529 kb on + strand at 5261.908 kb on - strand, within Pf1N1B4_4765 at 5261.908 kb on - strand, within Pf1N1B4_4765 at 5261.908 kb on - strand, within Pf1N1B4_4765 at 5261.962 kb on - strand, within Pf1N1B4_4765 at 5262.291 kb on + strand at 5262.291 kb on + strand, within Pf1N1B4_4765
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 5,259,313 - Pf1N1B4_4763 0.11 +0.8 5,259,702 - Pf1N1B4_4763 0.49 -0.5 5,259,793 + Pf1N1B4_4763 0.58 +0.4 5,259,793 + Pf1N1B4_4763 0.58 -0.2 5,259,793 + Pf1N1B4_4763 0.58 -2.0 5,259,793 + Pf1N1B4_4763 0.58 -0.9 5,259,793 + -0.5 5,259,794 - Pf1N1B4_4763 0.58 -0.5 5,259,915 + Pf1N1B4_4763 0.70 -2.0 5,259,983 + Pf1N1B4_4763 0.77 +1.1 5,259,984 - +0.5 5,260,199 + -1.8 5,260,199 + -0.8 5,260,200 - +0.5 5,260,235 - +2.1 5,260,235 - +0.1 5,260,352 + -0.5 5,260,353 - -0.5 5,260,353 - -0.5 5,260,353 - -0.9 5,260,401 - Pf1N1B4_4764 0.12 -0.3 5,260,401 - -0.5 5,260,517 + Pf1N1B4_4764 0.21 -1.5 5,260,517 + Pf1N1B4_4764 0.21 -0.5 5,260,518 - Pf1N1B4_4764 0.21 -0.6 5,260,518 - Pf1N1B4_4764 0.21 -1.5 5,260,550 + Pf1N1B4_4764 0.24 -2.5 5,260,550 + Pf1N1B4_4764 0.24 -0.5 5,260,593 - +0.5 5,260,593 - Pf1N1B4_4764 0.27 -0.5 5,260,700 + Pf1N1B4_4764 0.36 -2.0 5,260,981 - Pf1N1B4_4764 0.57 -0.5 5,261,315 + +1.1 5,261,315 + -1.0 5,261,315 + Pf1N1B4_4764 0.83 -2.5 5,261,316 - Pf1N1B4_4764 0.83 -1.5 5,261,435 + +1.1 5,261,436 - -0.5 5,261,465 + +0.4 5,261,493 - +0.5 5,261,493 - -0.0 5,261,493 - -0.5 5,261,493 - +0.5 5,261,493 - -0.1 5,261,493 - -1.5 5,261,493 - +0.5 5,261,529 + -0.5 5,261,908 - Pf1N1B4_4765 0.31 -0.5 5,261,908 - Pf1N1B4_4765 0.31 +0.5 5,261,908 - Pf1N1B4_4765 0.31 -1.0 5,261,962 - Pf1N1B4_4765 0.36 -0.2 5,262,291 + -0.5 5,262,291 + Pf1N1B4_4765 0.69 +0.2
Or see this region's nucleotide sequence