Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4145

Experiment: L-Isoleucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4145 and Pf1N1B4_4146 are separated by 27 nucleotides Pf1N1B4_4145 - hypothetical protein, at 4,590,825 to 4,590,992 _4145 Pf1N1B4_4146 - Transposase, at 4,591,020 to 4,591,148 _4146 Position (kb) 4590 4591Strain fitness (log2 ratio) -2 -1 0 1 2at 4589.839 kb on - strandat 4589.870 kb on - strandat 4589.896 kb on + strandat 4589.896 kb on + strandat 4589.897 kb on - strandat 4589.897 kb on - strandat 4589.897 kb on - strandat 4589.900 kb on - strandat 4590.095 kb on + strandat 4590.096 kb on - strandat 4590.096 kb on - strandat 4590.138 kb on - strandat 4590.140 kb on - strandat 4590.230 kb on - strandat 4590.268 kb on + strandat 4590.340 kb on - strandat 4590.740 kb on + strandat 4590.754 kb on - strandat 4590.800 kb on + strandat 4590.826 kb on + strandat 4590.895 kb on - strand, within Pf1N1B4_4145at 4590.895 kb on - strand, within Pf1N1B4_4145at 4591.146 kb on + strandat 4591.243 kb on + strandat 4591.391 kb on + strandat 4591.765 kb on - strandat 4591.884 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (N)
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4,589,839 - -0.9
4,589,870 - +1.0
4,589,896 + -0.3
4,589,896 + +0.1
4,589,897 - -1.9
4,589,897 - -0.3
4,589,897 - -2.7
4,589,900 - -0.9
4,590,095 + -1.3
4,590,096 - -1.3
4,590,096 - -0.9
4,590,138 - -0.8
4,590,140 - -2.3
4,590,230 - +0.4
4,590,268 + +0.9
4,590,340 - +1.7
4,590,740 + +2.0
4,590,754 - -0.3
4,590,800 + +0.7
4,590,826 + -0.9
4,590,895 - Pf1N1B4_4145 0.42 +0.3
4,590,895 - Pf1N1B4_4145 0.42 +1.2
4,591,146 + +1.2
4,591,243 + +0.5
4,591,391 + -0.9
4,591,765 - -0.3
4,591,884 + +0.2

Or see this region's nucleotide sequence