Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3702 and Pf1N1B4_3703 are separated by 165 nucleotides Pf1N1B4_3703 and Pf1N1B4_3704 are separated by 164 nucleotides Pf1N1B4_3704 and Pf1N1B4_3705 are separated by 68 nucleotides
Pf1N1B4_3702 - NLP/P60 family protein, at 4,111,158 to 4,111,721
_3702
Pf1N1B4_3703 - NLP/P60 family protein, at 4,111,887 to 4,112,423
_3703
Pf1N1B4_3704 - L-sorbosone dehydrogenase, at 4,112,588 to 4,113,901
_3704
Pf1N1B4_3705 - FIG00961250: hypothetical protein, at 4,113,970 to 4,114,554
_3705
Position (kb)
4112
4113
4114 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4111.635 kb on + strand, within Pf1N1B4_3702 at 4111.719 kb on + strand at 4111.734 kb on + strand at 4111.746 kb on - strand at 4111.753 kb on + strand at 4111.915 kb on + strand at 4111.916 kb on - strand at 4112.280 kb on + strand, within Pf1N1B4_3703 at 4112.280 kb on + strand, within Pf1N1B4_3703 at 4112.280 kb on + strand, within Pf1N1B4_3703 at 4112.280 kb on + strand at 4112.280 kb on + strand, within Pf1N1B4_3703 at 4112.280 kb on + strand, within Pf1N1B4_3703 at 4112.281 kb on - strand, within Pf1N1B4_3703 at 4112.281 kb on - strand at 4112.281 kb on - strand, within Pf1N1B4_3703 at 4112.356 kb on - strand, within Pf1N1B4_3703 at 4112.356 kb on - strand, within Pf1N1B4_3703 at 4112.370 kb on + strand at 4112.414 kb on + strand at 4112.414 kb on + strand at 4112.414 kb on + strand at 4112.522 kb on + strand at 4112.589 kb on + strand at 4112.589 kb on + strand at 4112.590 kb on - strand at 4112.590 kb on - strand at 4112.776 kb on + strand at 4112.776 kb on + strand, within Pf1N1B4_3704 at 4112.776 kb on + strand, within Pf1N1B4_3704 at 4112.777 kb on - strand, within Pf1N1B4_3704 at 4112.777 kb on - strand, within Pf1N1B4_3704 at 4112.980 kb on - strand, within Pf1N1B4_3704 at 4112.980 kb on - strand, within Pf1N1B4_3704 at 4112.980 kb on - strand, within Pf1N1B4_3704 at 4112.982 kb on + strand, within Pf1N1B4_3704 at 4112.982 kb on + strand, within Pf1N1B4_3704 at 4112.982 kb on + strand, within Pf1N1B4_3704 at 4112.982 kb on + strand, within Pf1N1B4_3704 at 4112.982 kb on + strand, within Pf1N1B4_3704 at 4112.982 kb on + strand, within Pf1N1B4_3704 at 4112.982 kb on + strand, within Pf1N1B4_3704 at 4112.982 kb on + strand, within Pf1N1B4_3704 at 4112.982 kb on + strand, within Pf1N1B4_3704 at 4112.983 kb on - strand, within Pf1N1B4_3704 at 4112.983 kb on - strand, within Pf1N1B4_3704 at 4112.983 kb on - strand, within Pf1N1B4_3704 at 4112.983 kb on - strand, within Pf1N1B4_3704 at 4112.983 kb on - strand, within Pf1N1B4_3704 at 4112.983 kb on - strand, within Pf1N1B4_3704 at 4112.983 kb on - strand, within Pf1N1B4_3704 at 4112.983 kb on - strand, within Pf1N1B4_3704 at 4112.989 kb on - strand, within Pf1N1B4_3704 at 4113.030 kb on + strand at 4113.030 kb on + strand, within Pf1N1B4_3704 at 4113.031 kb on - strand, within Pf1N1B4_3704 at 4113.031 kb on - strand, within Pf1N1B4_3704 at 4113.031 kb on - strand, within Pf1N1B4_3704 at 4113.031 kb on - strand, within Pf1N1B4_3704 at 4113.249 kb on + strand, within Pf1N1B4_3704 at 4113.249 kb on + strand, within Pf1N1B4_3704 at 4113.249 kb on + strand, within Pf1N1B4_3704 at 4113.250 kb on - strand, within Pf1N1B4_3704 at 4113.250 kb on - strand, within Pf1N1B4_3704 at 4113.250 kb on - strand, within Pf1N1B4_3704 at 4113.250 kb on - strand at 4113.250 kb on - strand, within Pf1N1B4_3704 at 4113.250 kb on - strand, within Pf1N1B4_3704 at 4113.250 kb on - strand, within Pf1N1B4_3704 at 4113.250 kb on - strand, within Pf1N1B4_3704 at 4113.405 kb on + strand, within Pf1N1B4_3704 at 4113.405 kb on + strand, within Pf1N1B4_3704 at 4113.405 kb on + strand, within Pf1N1B4_3704 at 4113.405 kb on + strand at 4113.405 kb on + strand, within Pf1N1B4_3704 at 4113.405 kb on + strand, within Pf1N1B4_3704 at 4113.406 kb on - strand, within Pf1N1B4_3704 at 4113.406 kb on - strand at 4113.406 kb on - strand, within Pf1N1B4_3704 at 4113.406 kb on - strand, within Pf1N1B4_3704 at 4113.461 kb on + strand, within Pf1N1B4_3704 at 4113.651 kb on + strand, within Pf1N1B4_3704 at 4113.651 kb on + strand, within Pf1N1B4_3704 at 4113.652 kb on - strand, within Pf1N1B4_3704 at 4113.652 kb on - strand, within Pf1N1B4_3704 at 4113.652 kb on - strand, within Pf1N1B4_3704 at 4113.652 kb on - strand, within Pf1N1B4_3704 at 4113.652 kb on - strand, within Pf1N1B4_3704 at 4113.652 kb on - strand, within Pf1N1B4_3704 at 4113.724 kb on + strand, within Pf1N1B4_3704 at 4113.724 kb on + strand, within Pf1N1B4_3704 at 4113.724 kb on + strand, within Pf1N1B4_3704 at 4113.724 kb on + strand, within Pf1N1B4_3704 at 4113.902 kb on + strand at 4113.903 kb on - strand at 4113.915 kb on + strand at 4113.916 kb on - strand at 4113.931 kb on + strand at 4113.931 kb on + strand at 4113.932 kb on - strand at 4113.932 kb on - strand at 4113.932 kb on - strand at 4113.932 kb on - strand at 4113.937 kb on + strand at 4113.937 kb on + strand at 4113.947 kb on + strand at 4113.948 kb on - strand at 4114.013 kb on + strand at 4114.014 kb on - strand at 4114.014 kb on - strand at 4114.014 kb on - strand at 4114.014 kb on - strand at 4114.050 kb on - strand, within Pf1N1B4_3705 at 4114.058 kb on + strand, within Pf1N1B4_3705 at 4114.058 kb on + strand, within Pf1N1B4_3705 at 4114.058 kb on + strand, within Pf1N1B4_3705 at 4114.059 kb on - strand, within Pf1N1B4_3705 at 4114.077 kb on + strand, within Pf1N1B4_3705 at 4114.077 kb on + strand, within Pf1N1B4_3705 at 4114.077 kb on + strand at 4114.077 kb on + strand, within Pf1N1B4_3705 at 4114.077 kb on + strand, within Pf1N1B4_3705 at 4114.077 kb on + strand, within Pf1N1B4_3705 at 4114.077 kb on + strand at 4114.078 kb on - strand, within Pf1N1B4_3705 at 4114.078 kb on - strand, within Pf1N1B4_3705 at 4114.187 kb on + strand, within Pf1N1B4_3705 at 4114.280 kb on + strand, within Pf1N1B4_3705 at 4114.280 kb on + strand, within Pf1N1B4_3705 at 4114.280 kb on + strand, within Pf1N1B4_3705 at 4114.440 kb on - strand, within Pf1N1B4_3705 at 4114.570 kb on + strand at 4114.570 kb on + strand at 4114.637 kb on + strand at 4114.638 kb on - strand at 4114.638 kb on - strand at 4114.655 kb on + strand at 4114.698 kb on + strand at 4114.846 kb on + strand at 4114.847 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 4,111,635 + Pf1N1B4_3702 0.85 -1.8 4,111,719 + -2.0 4,111,734 + -0.9 4,111,746 - -0.5 4,111,753 + +0.1 4,111,915 + +0.2 4,111,916 - -0.5 4,112,280 + Pf1N1B4_3703 0.73 -0.9 4,112,280 + Pf1N1B4_3703 0.73 -0.2 4,112,280 + Pf1N1B4_3703 0.73 -1.2 4,112,280 + -0.2 4,112,280 + Pf1N1B4_3703 0.73 -1.8 4,112,280 + Pf1N1B4_3703 0.73 -0.9 4,112,281 - Pf1N1B4_3703 0.73 -2.8 4,112,281 - +1.4 4,112,281 - Pf1N1B4_3703 0.73 -1.5 4,112,356 - Pf1N1B4_3703 0.87 -1.2 4,112,356 - Pf1N1B4_3703 0.87 +0.8 4,112,370 + -0.2 4,112,414 + -2.0 4,112,414 + -0.2 4,112,414 + -1.2 4,112,522 + -0.2 4,112,589 + -0.5 4,112,589 + +0.8 4,112,590 - +0.4 4,112,590 - -0.8 4,112,776 + -1.8 4,112,776 + Pf1N1B4_3704 0.14 +0.4 4,112,776 + Pf1N1B4_3704 0.14 -1.5 4,112,777 - Pf1N1B4_3704 0.14 +0.8 4,112,777 - Pf1N1B4_3704 0.14 -0.2 4,112,980 - Pf1N1B4_3704 0.30 -0.2 4,112,980 - Pf1N1B4_3704 0.30 +0.2 4,112,980 - Pf1N1B4_3704 0.30 -2.2 4,112,982 + Pf1N1B4_3704 0.30 -0.6 4,112,982 + Pf1N1B4_3704 0.30 -0.2 4,112,982 + Pf1N1B4_3704 0.30 -0.4 4,112,982 + Pf1N1B4_3704 0.30 +0.4 4,112,982 + Pf1N1B4_3704 0.30 -0.2 4,112,982 + Pf1N1B4_3704 0.30 -0.2 4,112,982 + Pf1N1B4_3704 0.30 -0.2 4,112,982 + Pf1N1B4_3704 0.30 -2.0 4,112,982 + Pf1N1B4_3704 0.30 -1.8 4,112,983 - Pf1N1B4_3704 0.30 +1.1 4,112,983 - Pf1N1B4_3704 0.30 -0.2 4,112,983 - Pf1N1B4_3704 0.30 -0.8 4,112,983 - Pf1N1B4_3704 0.30 -0.4 4,112,983 - Pf1N1B4_3704 0.30 +1.4 4,112,983 - Pf1N1B4_3704 0.30 +1.4 4,112,983 - Pf1N1B4_3704 0.30 -0.4 4,112,983 - Pf1N1B4_3704 0.30 -3.2 4,112,989 - Pf1N1B4_3704 0.31 -0.8 4,113,030 + +0.2 4,113,030 + Pf1N1B4_3704 0.34 +0.4 4,113,031 - Pf1N1B4_3704 0.34 -0.2 4,113,031 - Pf1N1B4_3704 0.34 +1.4 4,113,031 - Pf1N1B4_3704 0.34 +0.1 4,113,031 - Pf1N1B4_3704 0.34 -0.2 4,113,249 + Pf1N1B4_3704 0.50 +0.4 4,113,249 + Pf1N1B4_3704 0.50 -0.2 4,113,249 + Pf1N1B4_3704 0.50 +0.4 4,113,250 - Pf1N1B4_3704 0.50 +0.2 4,113,250 - Pf1N1B4_3704 0.50 +0.1 4,113,250 - Pf1N1B4_3704 0.50 -0.6 4,113,250 - -0.2 4,113,250 - Pf1N1B4_3704 0.50 +0.6 4,113,250 - Pf1N1B4_3704 0.50 -0.6 4,113,250 - Pf1N1B4_3704 0.50 -1.2 4,113,250 - Pf1N1B4_3704 0.50 -0.2 4,113,405 + Pf1N1B4_3704 0.62 +0.8 4,113,405 + Pf1N1B4_3704 0.62 -0.2 4,113,405 + Pf1N1B4_3704 0.62 +1.0 4,113,405 + -0.2 4,113,405 + Pf1N1B4_3704 0.62 -0.2 4,113,405 + Pf1N1B4_3704 0.62 -0.5 4,113,406 - Pf1N1B4_3704 0.62 +0.8 4,113,406 - -1.8 4,113,406 - Pf1N1B4_3704 0.62 +1.8 4,113,406 - Pf1N1B4_3704 0.62 -0.6 4,113,461 + Pf1N1B4_3704 0.66 -0.2 4,113,651 + Pf1N1B4_3704 0.81 +0.8 4,113,651 + Pf1N1B4_3704 0.81 -0.2 4,113,652 - Pf1N1B4_3704 0.81 -0.2 4,113,652 - Pf1N1B4_3704 0.81 -0.4 4,113,652 - Pf1N1B4_3704 0.81 +1.4 4,113,652 - Pf1N1B4_3704 0.81 +0.8 4,113,652 - Pf1N1B4_3704 0.81 -0.8 4,113,652 - Pf1N1B4_3704 0.81 -0.2 4,113,724 + Pf1N1B4_3704 0.86 +0.4 4,113,724 + Pf1N1B4_3704 0.86 +0.6 4,113,724 + Pf1N1B4_3704 0.86 +0.2 4,113,724 + Pf1N1B4_3704 0.86 -0.7 4,113,902 + -1.8 4,113,903 - -0.0 4,113,915 + -0.8 4,113,916 - +1.8 4,113,931 + +0.2 4,113,931 + +1.2 4,113,932 - +1.4 4,113,932 - -0.6 4,113,932 - -0.2 4,113,932 - +0.4 4,113,937 + -1.2 4,113,937 + +1.8 4,113,947 + -1.8 4,113,948 - +0.8 4,114,013 + -2.2 4,114,014 - +1.4 4,114,014 - +0.4 4,114,014 - -0.2 4,114,014 - -0.2 4,114,050 - Pf1N1B4_3705 0.14 +0.2 4,114,058 + Pf1N1B4_3705 0.15 -1.5 4,114,058 + Pf1N1B4_3705 0.15 -2.8 4,114,058 + Pf1N1B4_3705 0.15 -1.2 4,114,059 - Pf1N1B4_3705 0.15 +0.4 4,114,077 + Pf1N1B4_3705 0.18 +1.4 4,114,077 + Pf1N1B4_3705 0.18 +0.8 4,114,077 + +1.4 4,114,077 + Pf1N1B4_3705 0.18 -0.7 4,114,077 + Pf1N1B4_3705 0.18 -1.2 4,114,077 + Pf1N1B4_3705 0.18 -0.0 4,114,077 + +1.4 4,114,078 - Pf1N1B4_3705 0.18 +0.2 4,114,078 - Pf1N1B4_3705 0.18 +2.2 4,114,187 + Pf1N1B4_3705 0.37 -0.8 4,114,280 + Pf1N1B4_3705 0.53 -0.8 4,114,280 + Pf1N1B4_3705 0.53 -2.2 4,114,280 + Pf1N1B4_3705 0.53 +0.1 4,114,440 - Pf1N1B4_3705 0.80 -0.1 4,114,570 + -0.2 4,114,570 + -1.2 4,114,637 + +0.3 4,114,638 - +1.8 4,114,638 - -0.6 4,114,655 + +0.8 4,114,698 + -1.2 4,114,846 + +1.2 4,114,847 - -0.7
Or see this region's nucleotide sequence