Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3698 and Pf1N1B4_3699 are separated by 389 nucleotides Pf1N1B4_3699 and Pf1N1B4_3700 are separated by 30 nucleotides
Pf1N1B4_3698 - Phosphoribosylformylglycinamidine cyclo-ligase (EC 6.3.3.1), at 4,106,311 to 4,107,369
_3698
Pf1N1B4_3699 - FIG00954480: hypothetical protein, at 4,107,759 to 4,108,817
_3699
Pf1N1B4_3700 - Putative permease often clustered with de novo purine synthesis, at 4,108,848 to 4,109,921
_3700
Position (kb)
4107
4108
4109 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4106.925 kb on - strand at 4107.604 kb on + strand at 4107.604 kb on + strand at 4107.604 kb on + strand at 4107.604 kb on + strand at 4107.604 kb on + strand at 4107.605 kb on - strand at 4107.605 kb on - strand at 4107.605 kb on - strand at 4107.606 kb on + strand at 4107.606 kb on + strand at 4107.607 kb on - strand at 4107.634 kb on + strand at 4108.397 kb on - strand, within Pf1N1B4_3699 at 4108.397 kb on - strand, within Pf1N1B4_3699 at 4108.826 kb on + strand at 4108.827 kb on - strand at 4108.827 kb on - strand at 4108.827 kb on - strand at 4108.828 kb on + strand at 4108.828 kb on + strand at 4108.828 kb on + strand at 4108.829 kb on - strand at 4108.830 kb on + strand at 4108.831 kb on - strand at 4108.831 kb on - strand at 4108.845 kb on + strand at 4108.845 kb on + strand at 4108.908 kb on + strand at 4108.959 kb on + strand, within Pf1N1B4_3700 at 4108.960 kb on - strand, within Pf1N1B4_3700 at 4108.960 kb on - strand, within Pf1N1B4_3700 at 4109.110 kb on - strand, within Pf1N1B4_3700 at 4109.110 kb on - strand, within Pf1N1B4_3700 at 4109.110 kb on - strand, within Pf1N1B4_3700 at 4109.505 kb on + strand, within Pf1N1B4_3700 at 4109.506 kb on - strand, within Pf1N1B4_3700 at 4109.506 kb on - strand at 4109.586 kb on + strand, within Pf1N1B4_3700 at 4109.587 kb on - strand, within Pf1N1B4_3700 at 4109.587 kb on - strand, within Pf1N1B4_3700 at 4109.656 kb on - strand, within Pf1N1B4_3700
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 4,106,925 - -1.8 4,107,604 + -0.6 4,107,604 + +0.4 4,107,604 + -1.0 4,107,604 + -0.2 4,107,604 + -1.8 4,107,605 - -0.2 4,107,605 - +2.1 4,107,605 - -0.6 4,107,606 + +0.8 4,107,606 + +0.8 4,107,607 - +0.8 4,107,634 + +0.1 4,108,397 - Pf1N1B4_3699 0.60 +1.1 4,108,397 - Pf1N1B4_3699 0.60 -0.2 4,108,826 + -0.2 4,108,827 - -1.8 4,108,827 - -1.8 4,108,827 - -0.2 4,108,828 + +0.2 4,108,828 + +0.3 4,108,828 + -1.8 4,108,829 - +0.2 4,108,830 + +0.3 4,108,831 - +0.4 4,108,831 - -0.6 4,108,845 + -1.0 4,108,845 + +0.1 4,108,908 + -1.5 4,108,959 + Pf1N1B4_3700 0.10 +0.1 4,108,960 - Pf1N1B4_3700 0.10 -1.2 4,108,960 - Pf1N1B4_3700 0.10 -1.3 4,109,110 - Pf1N1B4_3700 0.24 +1.0 4,109,110 - Pf1N1B4_3700 0.24 -0.7 4,109,110 - Pf1N1B4_3700 0.24 +0.8 4,109,505 + Pf1N1B4_3700 0.61 -2.5 4,109,506 - Pf1N1B4_3700 0.61 +0.2 4,109,506 - +0.4 4,109,586 + Pf1N1B4_3700 0.69 -0.8 4,109,587 - Pf1N1B4_3700 0.69 +0.8 4,109,587 - Pf1N1B4_3700 0.69 -1.5 4,109,656 - Pf1N1B4_3700 0.75 -0.8
Or see this region's nucleotide sequence