Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3501 and Pf1N1B4_3502 are separated by 23 nucleotides Pf1N1B4_3502 and Pf1N1B4_3503 overlap by 21 nucleotides Pf1N1B4_3503 and Pf1N1B4_3504 are separated by 1 nucleotides
Pf1N1B4_3501 - BarA sensory histidine kinase (= VarS = GacS), at 3,905,307 to 3,908,057
_3501
Pf1N1B4_3502 - hypothetical protein, at 3,908,081 to 3,908,347
_3502
Pf1N1B4_3503 - Two-component response regulator PfeR, enterobactin, at 3,908,327 to 3,908,995
_3503
Pf1N1B4_3504 - Two-component sensor histidine kinase PfeS, enterobactin, at 3,908,997 to 3,910,412
_3504
Position (kb)
3908
3909 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3907.973 kb on - strand at 3908.037 kb on - strand at 3908.037 kb on - strand at 3908.037 kb on - strand at 3908.037 kb on - strand at 3908.082 kb on + strand at 3908.339 kb on + strand at 3908.339 kb on + strand at 3908.339 kb on + strand at 3908.339 kb on + strand at 3908.340 kb on - strand at 3908.585 kb on + strand, within Pf1N1B4_3503 at 3908.786 kb on - strand, within Pf1N1B4_3503 at 3908.786 kb on - strand, within Pf1N1B4_3503 at 3908.786 kb on - strand, within Pf1N1B4_3503 at 3908.786 kb on - strand, within Pf1N1B4_3503 at 3908.811 kb on + strand, within Pf1N1B4_3503 at 3908.811 kb on + strand, within Pf1N1B4_3503 at 3908.811 kb on + strand, within Pf1N1B4_3503 at 3908.811 kb on + strand at 3908.812 kb on - strand at 3908.812 kb on - strand, within Pf1N1B4_3503 at 3908.812 kb on - strand, within Pf1N1B4_3503 at 3908.812 kb on - strand, within Pf1N1B4_3503 at 3908.841 kb on + strand, within Pf1N1B4_3503 at 3908.841 kb on + strand, within Pf1N1B4_3503 at 3908.945 kb on - strand at 3908.963 kb on + strand at 3908.967 kb on + strand at 3908.967 kb on + strand at 3908.967 kb on + strand at 3908.967 kb on + strand at 3908.968 kb on - strand at 3908.968 kb on - strand at 3909.090 kb on + strand at 3909.091 kb on - strand at 3909.140 kb on + strand, within Pf1N1B4_3504 at 3909.434 kb on + strand, within Pf1N1B4_3504 at 3909.434 kb on + strand, within Pf1N1B4_3504 at 3909.435 kb on - strand, within Pf1N1B4_3504 at 3909.662 kb on + strand, within Pf1N1B4_3504 at 3909.662 kb on + strand, within Pf1N1B4_3504 at 3909.663 kb on - strand, within Pf1N1B4_3504 at 3909.942 kb on + strand, within Pf1N1B4_3504 at 3909.942 kb on + strand, within Pf1N1B4_3504
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,907,973 - +0.1 3,908,037 - -1.5 3,908,037 - +1.8 3,908,037 - -0.2 3,908,037 - -0.2 3,908,082 + +0.4 3,908,339 + -0.5 3,908,339 + +1.4 3,908,339 + -0.6 3,908,339 + -0.2 3,908,340 - +1.2 3,908,585 + Pf1N1B4_3503 0.39 -0.2 3,908,786 - Pf1N1B4_3503 0.69 -1.2 3,908,786 - Pf1N1B4_3503 0.69 -3.3 3,908,786 - Pf1N1B4_3503 0.69 -1.7 3,908,786 - Pf1N1B4_3503 0.69 -2.3 3,908,811 + Pf1N1B4_3503 0.72 -1.0 3,908,811 + Pf1N1B4_3503 0.72 +0.8 3,908,811 + Pf1N1B4_3503 0.72 -0.5 3,908,811 + -1.7 3,908,812 - +0.8 3,908,812 - Pf1N1B4_3503 0.72 +0.8 3,908,812 - Pf1N1B4_3503 0.72 -1.2 3,908,812 - Pf1N1B4_3503 0.72 -0.6 3,908,841 + Pf1N1B4_3503 0.77 -0.2 3,908,841 + Pf1N1B4_3503 0.77 -0.7 3,908,945 - -0.2 3,908,963 + -0.7 3,908,967 + -2.7 3,908,967 + -0.2 3,908,967 + -0.2 3,908,967 + -0.4 3,908,968 - -0.1 3,908,968 - +1.8 3,909,090 + -0.9 3,909,091 - +2.2 3,909,140 + Pf1N1B4_3504 0.10 -0.4 3,909,434 + Pf1N1B4_3504 0.31 -0.9 3,909,434 + Pf1N1B4_3504 0.31 -0.6 3,909,435 - Pf1N1B4_3504 0.31 -0.1 3,909,662 + Pf1N1B4_3504 0.47 +0.3 3,909,662 + Pf1N1B4_3504 0.47 -2.5 3,909,663 - Pf1N1B4_3504 0.47 +0.3 3,909,942 + Pf1N1B4_3504 0.67 -0.6 3,909,942 + Pf1N1B4_3504 0.67 -2.5
Or see this region's nucleotide sequence