Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3503

Experiment: L-Isoleucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3501 and Pf1N1B4_3502 are separated by 23 nucleotidesPf1N1B4_3502 and Pf1N1B4_3503 overlap by 21 nucleotidesPf1N1B4_3503 and Pf1N1B4_3504 are separated by 1 nucleotides Pf1N1B4_3501 - BarA sensory histidine kinase (= VarS = GacS), at 3,905,307 to 3,908,057 _3501 Pf1N1B4_3502 - hypothetical protein, at 3,908,081 to 3,908,347 _3502 Pf1N1B4_3503 - Two-component response regulator PfeR, enterobactin, at 3,908,327 to 3,908,995 _3503 Pf1N1B4_3504 - Two-component sensor histidine kinase PfeS, enterobactin, at 3,908,997 to 3,910,412 _3504 Position (kb) 3908 3909Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3907.973 kb on - strandat 3908.037 kb on - strandat 3908.037 kb on - strandat 3908.037 kb on - strandat 3908.037 kb on - strandat 3908.082 kb on + strandat 3908.339 kb on + strandat 3908.339 kb on + strandat 3908.339 kb on + strandat 3908.339 kb on + strandat 3908.340 kb on - strandat 3908.585 kb on + strand, within Pf1N1B4_3503at 3908.786 kb on - strand, within Pf1N1B4_3503at 3908.786 kb on - strand, within Pf1N1B4_3503at 3908.786 kb on - strand, within Pf1N1B4_3503at 3908.786 kb on - strand, within Pf1N1B4_3503at 3908.811 kb on + strand, within Pf1N1B4_3503at 3908.811 kb on + strand, within Pf1N1B4_3503at 3908.811 kb on + strand, within Pf1N1B4_3503at 3908.811 kb on + strandat 3908.812 kb on - strandat 3908.812 kb on - strand, within Pf1N1B4_3503at 3908.812 kb on - strand, within Pf1N1B4_3503at 3908.812 kb on - strand, within Pf1N1B4_3503at 3908.841 kb on + strand, within Pf1N1B4_3503at 3908.841 kb on + strand, within Pf1N1B4_3503at 3908.945 kb on - strandat 3908.963 kb on + strandat 3908.967 kb on + strandat 3908.967 kb on + strandat 3908.967 kb on + strandat 3908.967 kb on + strandat 3908.968 kb on - strandat 3908.968 kb on - strandat 3909.090 kb on + strandat 3909.091 kb on - strandat 3909.140 kb on + strand, within Pf1N1B4_3504at 3909.434 kb on + strand, within Pf1N1B4_3504at 3909.434 kb on + strand, within Pf1N1B4_3504at 3909.435 kb on - strand, within Pf1N1B4_3504at 3909.662 kb on + strand, within Pf1N1B4_3504at 3909.662 kb on + strand, within Pf1N1B4_3504at 3909.663 kb on - strand, within Pf1N1B4_3504at 3909.942 kb on + strand, within Pf1N1B4_3504at 3909.942 kb on + strand, within Pf1N1B4_3504

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (N)
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3,907,973 - +0.1
3,908,037 - -1.5
3,908,037 - +1.8
3,908,037 - -0.2
3,908,037 - -0.2
3,908,082 + +0.4
3,908,339 + -0.5
3,908,339 + +1.4
3,908,339 + -0.6
3,908,339 + -0.2
3,908,340 - +1.2
3,908,585 + Pf1N1B4_3503 0.39 -0.2
3,908,786 - Pf1N1B4_3503 0.69 -1.2
3,908,786 - Pf1N1B4_3503 0.69 -3.3
3,908,786 - Pf1N1B4_3503 0.69 -1.7
3,908,786 - Pf1N1B4_3503 0.69 -2.3
3,908,811 + Pf1N1B4_3503 0.72 -1.0
3,908,811 + Pf1N1B4_3503 0.72 +0.8
3,908,811 + Pf1N1B4_3503 0.72 -0.5
3,908,811 + -1.7
3,908,812 - +0.8
3,908,812 - Pf1N1B4_3503 0.72 +0.8
3,908,812 - Pf1N1B4_3503 0.72 -1.2
3,908,812 - Pf1N1B4_3503 0.72 -0.6
3,908,841 + Pf1N1B4_3503 0.77 -0.2
3,908,841 + Pf1N1B4_3503 0.77 -0.7
3,908,945 - -0.2
3,908,963 + -0.7
3,908,967 + -2.7
3,908,967 + -0.2
3,908,967 + -0.2
3,908,967 + -0.4
3,908,968 - -0.1
3,908,968 - +1.8
3,909,090 + -0.9
3,909,091 - +2.2
3,909,140 + Pf1N1B4_3504 0.10 -0.4
3,909,434 + Pf1N1B4_3504 0.31 -0.9
3,909,434 + Pf1N1B4_3504 0.31 -0.6
3,909,435 - Pf1N1B4_3504 0.31 -0.1
3,909,662 + Pf1N1B4_3504 0.47 +0.3
3,909,662 + Pf1N1B4_3504 0.47 -2.5
3,909,663 - Pf1N1B4_3504 0.47 +0.3
3,909,942 + Pf1N1B4_3504 0.67 -0.6
3,909,942 + Pf1N1B4_3504 0.67 -2.5

Or see this region's nucleotide sequence