Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3139 and Pf1N1B4_3140 are separated by 253 nucleotides Pf1N1B4_3140 and Pf1N1B4_3141 overlap by 19 nucleotides Pf1N1B4_3141 and Pf1N1B4_3142 are separated by 80 nucleotides Pf1N1B4_3142 and Pf1N1B4_3143 are separated by 83 nucleotides
Pf1N1B4_3139 - Prophage tail fiber protein, at 3,522,593 to 3,523,801
_3139
Pf1N1B4_3140 - FIG101079: Lytic enzyme, at 3,524,055 to 3,524,585
_3140
Pf1N1B4_3141 - FIG00959768: hypothetical protein, at 3,524,567 to 3,525,085
_3141
Pf1N1B4_3142 - C-terminal domain of CinA type S; Protein Implicated in DNA repair function with RecA and MutS, at 3,525,166 to 3,525,666
_3142
Pf1N1B4_3143 - RecA protein, at 3,525,750 to 3,526,802
_3143
Position (kb)
3524
3525
3526 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3523.593 kb on + strand, within Pf1N1B4_3139 at 3523.797 kb on + strand at 3523.797 kb on + strand at 3523.797 kb on + strand at 3523.797 kb on + strand at 3523.797 kb on + strand at 3523.797 kb on + strand at 3523.797 kb on + strand at 3523.797 kb on + strand at 3523.797 kb on + strand at 3523.797 kb on + strand at 3523.797 kb on + strand at 3523.797 kb on + strand at 3523.797 kb on + strand at 3523.797 kb on + strand at 3523.798 kb on - strand at 3523.799 kb on + strand at 3523.799 kb on + strand at 3523.800 kb on - strand at 3523.820 kb on + strand at 3523.820 kb on + strand at 3523.827 kb on + strand at 3523.842 kb on + strand at 3523.842 kb on + strand at 3523.842 kb on + strand at 3523.843 kb on - strand at 3523.867 kb on + strand at 3523.905 kb on - strand at 3524.005 kb on + strand at 3524.021 kb on + strand at 3524.021 kb on + strand at 3524.021 kb on + strand at 3524.021 kb on + strand at 3524.021 kb on + strand at 3524.021 kb on + strand at 3524.021 kb on + strand at 3524.021 kb on + strand at 3524.022 kb on - strand at 3524.022 kb on - strand at 3524.022 kb on - strand at 3524.022 kb on - strand at 3524.044 kb on + strand at 3524.044 kb on + strand at 3524.044 kb on + strand at 3524.044 kb on + strand at 3524.044 kb on + strand at 3524.044 kb on + strand at 3524.045 kb on - strand at 3524.185 kb on + strand, within Pf1N1B4_3140 at 3524.185 kb on + strand, within Pf1N1B4_3140 at 3524.185 kb on + strand, within Pf1N1B4_3140 at 3524.185 kb on + strand, within Pf1N1B4_3140 at 3524.185 kb on + strand, within Pf1N1B4_3140 at 3524.185 kb on + strand, within Pf1N1B4_3140 at 3524.185 kb on + strand, within Pf1N1B4_3140 at 3524.186 kb on - strand, within Pf1N1B4_3140 at 3524.186 kb on - strand, within Pf1N1B4_3140 at 3524.186 kb on - strand, within Pf1N1B4_3140 at 3524.186 kb on - strand, within Pf1N1B4_3140 at 3524.186 kb on - strand, within Pf1N1B4_3140 at 3524.186 kb on - strand, within Pf1N1B4_3140 at 3524.186 kb on - strand, within Pf1N1B4_3140 at 3524.186 kb on - strand, within Pf1N1B4_3140 at 3524.190 kb on + strand, within Pf1N1B4_3140 at 3524.190 kb on + strand, within Pf1N1B4_3140 at 3524.190 kb on + strand, within Pf1N1B4_3140 at 3524.190 kb on + strand, within Pf1N1B4_3140 at 3524.190 kb on + strand, within Pf1N1B4_3140 at 3524.191 kb on - strand at 3524.191 kb on - strand, within Pf1N1B4_3140 at 3524.191 kb on - strand, within Pf1N1B4_3140 at 3524.191 kb on - strand, within Pf1N1B4_3140 at 3524.194 kb on + strand, within Pf1N1B4_3140 at 3524.194 kb on + strand, within Pf1N1B4_3140 at 3524.194 kb on + strand, within Pf1N1B4_3140 at 3524.194 kb on + strand, within Pf1N1B4_3140 at 3524.194 kb on + strand, within Pf1N1B4_3140 at 3524.194 kb on + strand, within Pf1N1B4_3140 at 3524.194 kb on + strand, within Pf1N1B4_3140 at 3524.194 kb on + strand, within Pf1N1B4_3140 at 3524.194 kb on + strand, within Pf1N1B4_3140 at 3524.195 kb on - strand, within Pf1N1B4_3140 at 3524.195 kb on - strand, within Pf1N1B4_3140 at 3524.195 kb on - strand, within Pf1N1B4_3140 at 3524.195 kb on - strand, within Pf1N1B4_3140 at 3524.195 kb on - strand, within Pf1N1B4_3140 at 3524.195 kb on - strand, within Pf1N1B4_3140 at 3524.195 kb on - strand, within Pf1N1B4_3140 at 3524.195 kb on - strand, within Pf1N1B4_3140 at 3524.195 kb on - strand, within Pf1N1B4_3140 at 3524.218 kb on - strand, within Pf1N1B4_3140 at 3524.229 kb on + strand, within Pf1N1B4_3140 at 3524.229 kb on + strand, within Pf1N1B4_3140 at 3524.229 kb on + strand at 3524.230 kb on - strand, within Pf1N1B4_3140 at 3524.230 kb on - strand, within Pf1N1B4_3140 at 3524.295 kb on + strand, within Pf1N1B4_3140 at 3524.296 kb on - strand, within Pf1N1B4_3140 at 3524.566 kb on + strand at 3524.566 kb on + strand at 3524.567 kb on - strand at 3524.711 kb on - strand, within Pf1N1B4_3141 at 3524.818 kb on + strand, within Pf1N1B4_3141 at 3524.819 kb on - strand, within Pf1N1B4_3141 at 3525.005 kb on - strand at 3525.005 kb on - strand at 3525.062 kb on + strand at 3525.062 kb on + strand at 3525.062 kb on + strand at 3525.062 kb on + strand at 3525.063 kb on - strand at 3525.063 kb on - strand at 3525.063 kb on - strand at 3525.063 kb on - strand at 3525.130 kb on - strand at 3525.174 kb on + strand at 3525.174 kb on + strand at 3525.174 kb on + strand at 3525.316 kb on + strand, within Pf1N1B4_3142 at 3525.317 kb on - strand, within Pf1N1B4_3142 at 3525.325 kb on + strand, within Pf1N1B4_3142 at 3525.325 kb on + strand, within Pf1N1B4_3142 at 3525.325 kb on + strand at 3525.326 kb on - strand, within Pf1N1B4_3142 at 3525.326 kb on - strand, within Pf1N1B4_3142 at 3525.326 kb on - strand, within Pf1N1B4_3142 at 3525.473 kb on + strand, within Pf1N1B4_3142 at 3525.474 kb on - strand, within Pf1N1B4_3142 at 3525.565 kb on + strand, within Pf1N1B4_3142 at 3525.565 kb on + strand, within Pf1N1B4_3142 at 3525.565 kb on + strand, within Pf1N1B4_3142 at 3525.728 kb on + strand at 3525.729 kb on - strand at 3525.735 kb on + strand at 3525.735 kb on + strand at 3525.735 kb on + strand at 3525.735 kb on + strand at 3525.939 kb on + strand, within Pf1N1B4_3143 at 3525.939 kb on + strand, within Pf1N1B4_3143
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,523,593 + Pf1N1B4_3139 0.83 -2.0 3,523,797 + +1.2 3,523,797 + +1.0 3,523,797 + +1.0 3,523,797 + +0.6 3,523,797 + -0.0 3,523,797 + -1.0 3,523,797 + +0.2 3,523,797 + -2.0 3,523,797 + -0.9 3,523,797 + +1.0 3,523,797 + -0.9 3,523,797 + +0.3 3,523,797 + -1.0 3,523,797 + -0.3 3,523,798 - -0.3 3,523,799 + -1.0 3,523,799 + -1.0 3,523,800 - -0.0 3,523,820 + +1.6 3,523,820 + -0.0 3,523,827 + -1.2 3,523,842 + +0.6 3,523,842 + +0.3 3,523,842 + -0.0 3,523,843 - -1.0 3,523,867 + -0.4 3,523,905 - +0.8 3,524,005 + +0.9 3,524,021 + -0.6 3,524,021 + -1.2 3,524,021 + +1.0 3,524,021 + -2.0 3,524,021 + -1.1 3,524,021 + -1.3 3,524,021 + -1.0 3,524,021 + -0.6 3,524,022 - -0.0 3,524,022 - +0.3 3,524,022 - -0.0 3,524,022 - +2.0 3,524,044 + -0.8 3,524,044 + -0.8 3,524,044 + -0.6 3,524,044 + -2.0 3,524,044 + -0.0 3,524,044 + +0.3 3,524,045 - -0.4 3,524,185 + Pf1N1B4_3140 0.24 -0.0 3,524,185 + Pf1N1B4_3140 0.24 -0.3 3,524,185 + Pf1N1B4_3140 0.24 -1.6 3,524,185 + Pf1N1B4_3140 0.24 -0.0 3,524,185 + Pf1N1B4_3140 0.24 -0.4 3,524,185 + Pf1N1B4_3140 0.24 -0.4 3,524,185 + Pf1N1B4_3140 0.24 -1.0 3,524,186 - Pf1N1B4_3140 0.25 +0.6 3,524,186 - Pf1N1B4_3140 0.25 -0.0 3,524,186 - Pf1N1B4_3140 0.25 -0.6 3,524,186 - Pf1N1B4_3140 0.25 -0.6 3,524,186 - Pf1N1B4_3140 0.25 -0.0 3,524,186 - Pf1N1B4_3140 0.25 +0.5 3,524,186 - Pf1N1B4_3140 0.25 -0.6 3,524,186 - Pf1N1B4_3140 0.25 -0.6 3,524,190 + Pf1N1B4_3140 0.25 -1.0 3,524,190 + Pf1N1B4_3140 0.25 -0.8 3,524,190 + Pf1N1B4_3140 0.25 -0.0 3,524,190 + Pf1N1B4_3140 0.25 -1.6 3,524,190 + Pf1N1B4_3140 0.25 -0.0 3,524,191 - -0.0 3,524,191 - Pf1N1B4_3140 0.26 -0.0 3,524,191 - Pf1N1B4_3140 0.26 +1.0 3,524,191 - Pf1N1B4_3140 0.26 -1.3 3,524,194 + Pf1N1B4_3140 0.26 -0.2 3,524,194 + Pf1N1B4_3140 0.26 -0.4 3,524,194 + Pf1N1B4_3140 0.26 -0.1 3,524,194 + Pf1N1B4_3140 0.26 +1.0 3,524,194 + Pf1N1B4_3140 0.26 -0.6 3,524,194 + Pf1N1B4_3140 0.26 -0.6 3,524,194 + Pf1N1B4_3140 0.26 +0.6 3,524,194 + Pf1N1B4_3140 0.26 -1.0 3,524,194 + Pf1N1B4_3140 0.26 +0.2 3,524,195 - Pf1N1B4_3140 0.26 -1.8 3,524,195 - Pf1N1B4_3140 0.26 -0.0 3,524,195 - Pf1N1B4_3140 0.26 +1.0 3,524,195 - Pf1N1B4_3140 0.26 -0.6 3,524,195 - Pf1N1B4_3140 0.26 +0.4 3,524,195 - Pf1N1B4_3140 0.26 +1.0 3,524,195 - Pf1N1B4_3140 0.26 -0.8 3,524,195 - Pf1N1B4_3140 0.26 -0.0 3,524,195 - Pf1N1B4_3140 0.26 -1.0 3,524,218 - Pf1N1B4_3140 0.31 -0.8 3,524,229 + Pf1N1B4_3140 0.33 -0.4 3,524,229 + Pf1N1B4_3140 0.33 -0.4 3,524,229 + -0.6 3,524,230 - Pf1N1B4_3140 0.33 -1.3 3,524,230 - Pf1N1B4_3140 0.33 -1.6 3,524,295 + Pf1N1B4_3140 0.45 +0.6 3,524,296 - Pf1N1B4_3140 0.45 +2.6 3,524,566 + -1.2 3,524,566 + +0.3 3,524,567 - -0.4 3,524,711 - Pf1N1B4_3141 0.28 -1.0 3,524,818 + Pf1N1B4_3141 0.48 -0.0 3,524,819 - Pf1N1B4_3141 0.49 -2.0 3,525,005 - +2.6 3,525,005 - +2.0 3,525,062 + -0.0 3,525,062 + -0.0 3,525,062 + -1.1 3,525,062 + -0.9 3,525,063 - -1.0 3,525,063 - -1.8 3,525,063 - +1.0 3,525,063 - -0.5 3,525,130 - -1.0 3,525,174 + -2.0 3,525,174 + -0.0 3,525,174 + -0.6 3,525,316 + Pf1N1B4_3142 0.30 -0.3 3,525,317 - Pf1N1B4_3142 0.30 -0.8 3,525,325 + Pf1N1B4_3142 0.32 -0.0 3,525,325 + Pf1N1B4_3142 0.32 +0.6 3,525,325 + -0.0 3,525,326 - Pf1N1B4_3142 0.32 -2.0 3,525,326 - Pf1N1B4_3142 0.32 -2.0 3,525,326 - Pf1N1B4_3142 0.32 -1.3 3,525,473 + Pf1N1B4_3142 0.61 -0.5 3,525,474 - Pf1N1B4_3142 0.61 -1.2 3,525,565 + Pf1N1B4_3142 0.80 -1.6 3,525,565 + Pf1N1B4_3142 0.80 -0.0 3,525,565 + Pf1N1B4_3142 0.80 +0.2 3,525,728 + +1.3 3,525,729 - -0.8 3,525,735 + -0.2 3,525,735 + -0.6 3,525,735 + -0.4 3,525,735 + -0.2 3,525,939 + Pf1N1B4_3143 0.18 -0.0 3,525,939 + Pf1N1B4_3143 0.18 +1.0
Or see this region's nucleotide sequence