Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2123

Experiment: L-Isoleucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2122 and Pf1N1B4_2123 overlap by 4 nucleotidesPf1N1B4_2123 and Pf1N1B4_2124 are separated by 65 nucleotidesPf1N1B4_2124 and Pf1N1B4_2125 are separated by 5 nucleotides Pf1N1B4_2122 - Phosphomannomutase (EC 5.4.2.8) / Phosphoglucomutase (EC 5.4.2.2), at 2,433,222 to 2,434,619 _2122 Pf1N1B4_2123 - hypothetical protein, at 2,434,616 to 2,434,816 _2123 Pf1N1B4_2124 - Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC 3.6.1.23), at 2,434,882 to 2,435,337 _2124 Pf1N1B4_2125 - Phosphopantothenoylcysteine decarboxylase (EC 4.1.1.36) / Phosphopantothenoylcysteine synthetase (EC 6.3.2.5), at 2,435,343 to 2,436,551 _2125 Position (kb) 2434 2435Strain fitness (log2 ratio) -3 -2 -1 0 1at 2433.626 kb on + strand, within Pf1N1B4_2122at 2433.626 kb on + strand, within Pf1N1B4_2122at 2433.626 kb on + strand, within Pf1N1B4_2122at 2433.626 kb on + strand, within Pf1N1B4_2122at 2433.626 kb on + strand, within Pf1N1B4_2122at 2433.627 kb on - strand, within Pf1N1B4_2122at 2433.627 kb on - strand, within Pf1N1B4_2122at 2433.627 kb on - strand, within Pf1N1B4_2122at 2434.138 kb on + strand, within Pf1N1B4_2122at 2434.138 kb on + strand, within Pf1N1B4_2122at 2434.138 kb on + strand, within Pf1N1B4_2122at 2434.139 kb on - strand, within Pf1N1B4_2122at 2434.139 kb on - strand, within Pf1N1B4_2122at 2434.273 kb on + strand, within Pf1N1B4_2122at 2434.273 kb on + strand, within Pf1N1B4_2122at 2434.274 kb on - strand, within Pf1N1B4_2122at 2434.274 kb on - strand, within Pf1N1B4_2122at 2434.326 kb on + strand, within Pf1N1B4_2122at 2434.326 kb on + strand, within Pf1N1B4_2122at 2434.326 kb on + strand, within Pf1N1B4_2122at 2434.326 kb on + strand, within Pf1N1B4_2122at 2434.326 kb on + strand, within Pf1N1B4_2122at 2434.326 kb on + strand, within Pf1N1B4_2122at 2434.326 kb on + strand, within Pf1N1B4_2122at 2434.326 kb on + strand, within Pf1N1B4_2122at 2434.326 kb on + strand, within Pf1N1B4_2122at 2434.326 kb on + strand, within Pf1N1B4_2122at 2434.326 kb on + strand, within Pf1N1B4_2122at 2434.327 kb on - strand, within Pf1N1B4_2122at 2434.327 kb on - strand, within Pf1N1B4_2122at 2434.327 kb on - strand, within Pf1N1B4_2122at 2434.327 kb on - strand, within Pf1N1B4_2122at 2434.327 kb on - strand, within Pf1N1B4_2122at 2434.339 kb on + strand, within Pf1N1B4_2122at 2434.339 kb on + strand, within Pf1N1B4_2122at 2434.340 kb on - strand, within Pf1N1B4_2122at 2434.340 kb on - strand, within Pf1N1B4_2122at 2434.340 kb on - strand, within Pf1N1B4_2122at 2434.340 kb on - strand, within Pf1N1B4_2122at 2434.340 kb on - strand, within Pf1N1B4_2122at 2434.342 kb on + strand, within Pf1N1B4_2122at 2434.342 kb on + strand, within Pf1N1B4_2122at 2434.342 kb on + strand, within Pf1N1B4_2122at 2434.343 kb on - strand, within Pf1N1B4_2122at 2434.343 kb on - strand, within Pf1N1B4_2122at 2434.343 kb on - strand, within Pf1N1B4_2122at 2434.343 kb on - strand, within Pf1N1B4_2122at 2434.461 kb on + strand, within Pf1N1B4_2122at 2434.461 kb on + strand, within Pf1N1B4_2122at 2434.462 kb on - strand, within Pf1N1B4_2122at 2434.462 kb on - strand, within Pf1N1B4_2122at 2434.516 kb on + strandat 2434.517 kb on - strandat 2434.517 kb on - strandat 2434.640 kb on + strand, within Pf1N1B4_2123at 2434.640 kb on + strand, within Pf1N1B4_2123at 2434.640 kb on + strand, within Pf1N1B4_2123at 2434.640 kb on + strand, within Pf1N1B4_2123at 2434.640 kb on + strand, within Pf1N1B4_2123at 2434.640 kb on + strand, within Pf1N1B4_2123at 2434.641 kb on - strand, within Pf1N1B4_2123at 2434.694 kb on + strand, within Pf1N1B4_2123at 2434.694 kb on + strand, within Pf1N1B4_2123at 2434.694 kb on + strandat 2434.694 kb on + strand, within Pf1N1B4_2123at 2434.694 kb on + strand, within Pf1N1B4_2123at 2434.694 kb on + strand, within Pf1N1B4_2123at 2434.695 kb on - strand, within Pf1N1B4_2123at 2434.729 kb on + strand, within Pf1N1B4_2123at 2434.729 kb on + strand, within Pf1N1B4_2123at 2434.729 kb on + strand, within Pf1N1B4_2123at 2434.729 kb on + strand, within Pf1N1B4_2123at 2434.730 kb on - strand, within Pf1N1B4_2123at 2434.730 kb on - strand, within Pf1N1B4_2123at 2434.790 kb on + strand, within Pf1N1B4_2123at 2434.791 kb on - strand, within Pf1N1B4_2123at 2434.828 kb on - strandat 2435.345 kb on - strandat 2435.345 kb on - strandat 2435.345 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (N)
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2,433,626 + Pf1N1B4_2122 0.29 +1.7
2,433,626 + Pf1N1B4_2122 0.29 +0.0
2,433,626 + Pf1N1B4_2122 0.29 +0.3
2,433,626 + Pf1N1B4_2122 0.29 +0.3
2,433,626 + Pf1N1B4_2122 0.29 -0.2
2,433,627 - Pf1N1B4_2122 0.29 +0.5
2,433,627 - Pf1N1B4_2122 0.29 +0.3
2,433,627 - Pf1N1B4_2122 0.29 +1.3
2,434,138 + Pf1N1B4_2122 0.66 -0.3
2,434,138 + Pf1N1B4_2122 0.66 -0.3
2,434,138 + Pf1N1B4_2122 0.66 +0.1
2,434,139 - Pf1N1B4_2122 0.66 -0.3
2,434,139 - Pf1N1B4_2122 0.66 +0.3
2,434,273 + Pf1N1B4_2122 0.75 -1.0
2,434,273 + Pf1N1B4_2122 0.75 -0.3
2,434,274 - Pf1N1B4_2122 0.75 -1.7
2,434,274 - Pf1N1B4_2122 0.75 -0.7
2,434,326 + Pf1N1B4_2122 0.79 -1.3
2,434,326 + Pf1N1B4_2122 0.79 +0.7
2,434,326 + Pf1N1B4_2122 0.79 -1.0
2,434,326 + Pf1N1B4_2122 0.79 -2.4
2,434,326 + Pf1N1B4_2122 0.79 -0.4
2,434,326 + Pf1N1B4_2122 0.79 +1.1
2,434,326 + Pf1N1B4_2122 0.79 -1.1
2,434,326 + Pf1N1B4_2122 0.79 +1.2
2,434,326 + Pf1N1B4_2122 0.79 -1.5
2,434,326 + Pf1N1B4_2122 0.79 -0.4
2,434,326 + Pf1N1B4_2122 0.79 -1.7
2,434,327 - Pf1N1B4_2122 0.79 -0.7
2,434,327 - Pf1N1B4_2122 0.79 +0.5
2,434,327 - Pf1N1B4_2122 0.79 -0.7
2,434,327 - Pf1N1B4_2122 0.79 -1.0
2,434,327 - Pf1N1B4_2122 0.79 +1.7
2,434,339 + Pf1N1B4_2122 0.80 +1.3
2,434,339 + Pf1N1B4_2122 0.80 -1.1
2,434,340 - Pf1N1B4_2122 0.80 +0.5
2,434,340 - Pf1N1B4_2122 0.80 +1.3
2,434,340 - Pf1N1B4_2122 0.80 -0.7
2,434,340 - Pf1N1B4_2122 0.80 +0.9
2,434,340 - Pf1N1B4_2122 0.80 -0.4
2,434,342 + Pf1N1B4_2122 0.80 -0.4
2,434,342 + Pf1N1B4_2122 0.80 -0.3
2,434,342 + Pf1N1B4_2122 0.80 -0.3
2,434,343 - Pf1N1B4_2122 0.80 -0.7
2,434,343 - Pf1N1B4_2122 0.80 +0.7
2,434,343 - Pf1N1B4_2122 0.80 -0.3
2,434,343 - Pf1N1B4_2122 0.80 -0.7
2,434,461 + Pf1N1B4_2122 0.89 -0.2
2,434,461 + Pf1N1B4_2122 0.89 +1.3
2,434,462 - Pf1N1B4_2122 0.89 -2.5
2,434,462 - Pf1N1B4_2122 0.89 -1.1
2,434,516 + -0.3
2,434,517 - -1.5
2,434,517 - +0.0
2,434,640 + Pf1N1B4_2123 0.12 -0.7
2,434,640 + Pf1N1B4_2123 0.12 -1.3
2,434,640 + Pf1N1B4_2123 0.12 +0.7
2,434,640 + Pf1N1B4_2123 0.12 -0.6
2,434,640 + Pf1N1B4_2123 0.12 -3.3
2,434,640 + Pf1N1B4_2123 0.12 -1.5
2,434,641 - Pf1N1B4_2123 0.12 -0.9
2,434,694 + Pf1N1B4_2123 0.39 -2.0
2,434,694 + Pf1N1B4_2123 0.39 -0.2
2,434,694 + -2.0
2,434,694 + Pf1N1B4_2123 0.39 -0.9
2,434,694 + Pf1N1B4_2123 0.39 +0.3
2,434,694 + Pf1N1B4_2123 0.39 -1.6
2,434,695 - Pf1N1B4_2123 0.39 -1.4
2,434,729 + Pf1N1B4_2123 0.56 -0.4
2,434,729 + Pf1N1B4_2123 0.56 -1.3
2,434,729 + Pf1N1B4_2123 0.56 -2.5
2,434,729 + Pf1N1B4_2123 0.56 +0.7
2,434,730 - Pf1N1B4_2123 0.57 -1.3
2,434,730 - Pf1N1B4_2123 0.57 -1.3
2,434,790 + Pf1N1B4_2123 0.87 -1.3
2,434,791 - Pf1N1B4_2123 0.87 -1.7
2,434,828 - +0.3
2,435,345 - -1.5
2,435,345 - -2.0
2,435,345 - +0.1

Or see this region's nucleotide sequence