Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2122 and Pf1N1B4_2123 overlap by 4 nucleotides Pf1N1B4_2123 and Pf1N1B4_2124 are separated by 65 nucleotides Pf1N1B4_2124 and Pf1N1B4_2125 are separated by 5 nucleotides
Pf1N1B4_2122 - Phosphomannomutase (EC 5.4.2.8) / Phosphoglucomutase (EC 5.4.2.2), at 2,433,222 to 2,434,619
_2122
Pf1N1B4_2123 - hypothetical protein, at 2,434,616 to 2,434,816
_2123
Pf1N1B4_2124 - Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC 3.6.1.23), at 2,434,882 to 2,435,337
_2124
Pf1N1B4_2125 - Phosphopantothenoylcysteine decarboxylase (EC 4.1.1.36) / Phosphopantothenoylcysteine synthetase (EC 6.3.2.5), at 2,435,343 to 2,436,551
_2125
Position (kb)
2434
2435 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2433.626 kb on + strand, within Pf1N1B4_2122 at 2433.626 kb on + strand, within Pf1N1B4_2122 at 2433.626 kb on + strand, within Pf1N1B4_2122 at 2433.626 kb on + strand, within Pf1N1B4_2122 at 2433.626 kb on + strand, within Pf1N1B4_2122 at 2433.627 kb on - strand, within Pf1N1B4_2122 at 2433.627 kb on - strand, within Pf1N1B4_2122 at 2433.627 kb on - strand, within Pf1N1B4_2122 at 2434.138 kb on + strand, within Pf1N1B4_2122 at 2434.138 kb on + strand, within Pf1N1B4_2122 at 2434.138 kb on + strand, within Pf1N1B4_2122 at 2434.139 kb on - strand, within Pf1N1B4_2122 at 2434.139 kb on - strand, within Pf1N1B4_2122 at 2434.273 kb on + strand, within Pf1N1B4_2122 at 2434.273 kb on + strand, within Pf1N1B4_2122 at 2434.274 kb on - strand, within Pf1N1B4_2122 at 2434.274 kb on - strand, within Pf1N1B4_2122 at 2434.326 kb on + strand, within Pf1N1B4_2122 at 2434.326 kb on + strand, within Pf1N1B4_2122 at 2434.326 kb on + strand, within Pf1N1B4_2122 at 2434.326 kb on + strand, within Pf1N1B4_2122 at 2434.326 kb on + strand, within Pf1N1B4_2122 at 2434.326 kb on + strand, within Pf1N1B4_2122 at 2434.326 kb on + strand, within Pf1N1B4_2122 at 2434.326 kb on + strand, within Pf1N1B4_2122 at 2434.326 kb on + strand, within Pf1N1B4_2122 at 2434.326 kb on + strand, within Pf1N1B4_2122 at 2434.326 kb on + strand, within Pf1N1B4_2122 at 2434.327 kb on - strand, within Pf1N1B4_2122 at 2434.327 kb on - strand, within Pf1N1B4_2122 at 2434.327 kb on - strand, within Pf1N1B4_2122 at 2434.327 kb on - strand, within Pf1N1B4_2122 at 2434.327 kb on - strand, within Pf1N1B4_2122 at 2434.339 kb on + strand, within Pf1N1B4_2122 at 2434.339 kb on + strand, within Pf1N1B4_2122 at 2434.340 kb on - strand, within Pf1N1B4_2122 at 2434.340 kb on - strand, within Pf1N1B4_2122 at 2434.340 kb on - strand, within Pf1N1B4_2122 at 2434.340 kb on - strand, within Pf1N1B4_2122 at 2434.340 kb on - strand, within Pf1N1B4_2122 at 2434.342 kb on + strand, within Pf1N1B4_2122 at 2434.342 kb on + strand, within Pf1N1B4_2122 at 2434.342 kb on + strand, within Pf1N1B4_2122 at 2434.343 kb on - strand, within Pf1N1B4_2122 at 2434.343 kb on - strand, within Pf1N1B4_2122 at 2434.343 kb on - strand, within Pf1N1B4_2122 at 2434.343 kb on - strand, within Pf1N1B4_2122 at 2434.461 kb on + strand, within Pf1N1B4_2122 at 2434.461 kb on + strand, within Pf1N1B4_2122 at 2434.462 kb on - strand, within Pf1N1B4_2122 at 2434.462 kb on - strand, within Pf1N1B4_2122 at 2434.516 kb on + strand at 2434.517 kb on - strand at 2434.517 kb on - strand at 2434.640 kb on + strand, within Pf1N1B4_2123 at 2434.640 kb on + strand, within Pf1N1B4_2123 at 2434.640 kb on + strand, within Pf1N1B4_2123 at 2434.640 kb on + strand, within Pf1N1B4_2123 at 2434.640 kb on + strand, within Pf1N1B4_2123 at 2434.640 kb on + strand, within Pf1N1B4_2123 at 2434.641 kb on - strand, within Pf1N1B4_2123 at 2434.694 kb on + strand, within Pf1N1B4_2123 at 2434.694 kb on + strand, within Pf1N1B4_2123 at 2434.694 kb on + strand at 2434.694 kb on + strand, within Pf1N1B4_2123 at 2434.694 kb on + strand, within Pf1N1B4_2123 at 2434.694 kb on + strand, within Pf1N1B4_2123 at 2434.695 kb on - strand, within Pf1N1B4_2123 at 2434.729 kb on + strand, within Pf1N1B4_2123 at 2434.729 kb on + strand, within Pf1N1B4_2123 at 2434.729 kb on + strand, within Pf1N1B4_2123 at 2434.729 kb on + strand, within Pf1N1B4_2123 at 2434.730 kb on - strand, within Pf1N1B4_2123 at 2434.730 kb on - strand, within Pf1N1B4_2123 at 2434.790 kb on + strand, within Pf1N1B4_2123 at 2434.791 kb on - strand, within Pf1N1B4_2123 at 2434.828 kb on - strand at 2435.345 kb on - strand at 2435.345 kb on - strand at 2435.345 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,433,626 + Pf1N1B4_2122 0.29 +1.7 2,433,626 + Pf1N1B4_2122 0.29 +0.0 2,433,626 + Pf1N1B4_2122 0.29 +0.3 2,433,626 + Pf1N1B4_2122 0.29 +0.3 2,433,626 + Pf1N1B4_2122 0.29 -0.2 2,433,627 - Pf1N1B4_2122 0.29 +0.5 2,433,627 - Pf1N1B4_2122 0.29 +0.3 2,433,627 - Pf1N1B4_2122 0.29 +1.3 2,434,138 + Pf1N1B4_2122 0.66 -0.3 2,434,138 + Pf1N1B4_2122 0.66 -0.3 2,434,138 + Pf1N1B4_2122 0.66 +0.1 2,434,139 - Pf1N1B4_2122 0.66 -0.3 2,434,139 - Pf1N1B4_2122 0.66 +0.3 2,434,273 + Pf1N1B4_2122 0.75 -1.0 2,434,273 + Pf1N1B4_2122 0.75 -0.3 2,434,274 - Pf1N1B4_2122 0.75 -1.7 2,434,274 - Pf1N1B4_2122 0.75 -0.7 2,434,326 + Pf1N1B4_2122 0.79 -1.3 2,434,326 + Pf1N1B4_2122 0.79 +0.7 2,434,326 + Pf1N1B4_2122 0.79 -1.0 2,434,326 + Pf1N1B4_2122 0.79 -2.4 2,434,326 + Pf1N1B4_2122 0.79 -0.4 2,434,326 + Pf1N1B4_2122 0.79 +1.1 2,434,326 + Pf1N1B4_2122 0.79 -1.1 2,434,326 + Pf1N1B4_2122 0.79 +1.2 2,434,326 + Pf1N1B4_2122 0.79 -1.5 2,434,326 + Pf1N1B4_2122 0.79 -0.4 2,434,326 + Pf1N1B4_2122 0.79 -1.7 2,434,327 - Pf1N1B4_2122 0.79 -0.7 2,434,327 - Pf1N1B4_2122 0.79 +0.5 2,434,327 - Pf1N1B4_2122 0.79 -0.7 2,434,327 - Pf1N1B4_2122 0.79 -1.0 2,434,327 - Pf1N1B4_2122 0.79 +1.7 2,434,339 + Pf1N1B4_2122 0.80 +1.3 2,434,339 + Pf1N1B4_2122 0.80 -1.1 2,434,340 - Pf1N1B4_2122 0.80 +0.5 2,434,340 - Pf1N1B4_2122 0.80 +1.3 2,434,340 - Pf1N1B4_2122 0.80 -0.7 2,434,340 - Pf1N1B4_2122 0.80 +0.9 2,434,340 - Pf1N1B4_2122 0.80 -0.4 2,434,342 + Pf1N1B4_2122 0.80 -0.4 2,434,342 + Pf1N1B4_2122 0.80 -0.3 2,434,342 + Pf1N1B4_2122 0.80 -0.3 2,434,343 - Pf1N1B4_2122 0.80 -0.7 2,434,343 - Pf1N1B4_2122 0.80 +0.7 2,434,343 - Pf1N1B4_2122 0.80 -0.3 2,434,343 - Pf1N1B4_2122 0.80 -0.7 2,434,461 + Pf1N1B4_2122 0.89 -0.2 2,434,461 + Pf1N1B4_2122 0.89 +1.3 2,434,462 - Pf1N1B4_2122 0.89 -2.5 2,434,462 - Pf1N1B4_2122 0.89 -1.1 2,434,516 + -0.3 2,434,517 - -1.5 2,434,517 - +0.0 2,434,640 + Pf1N1B4_2123 0.12 -0.7 2,434,640 + Pf1N1B4_2123 0.12 -1.3 2,434,640 + Pf1N1B4_2123 0.12 +0.7 2,434,640 + Pf1N1B4_2123 0.12 -0.6 2,434,640 + Pf1N1B4_2123 0.12 -3.3 2,434,640 + Pf1N1B4_2123 0.12 -1.5 2,434,641 - Pf1N1B4_2123 0.12 -0.9 2,434,694 + Pf1N1B4_2123 0.39 -2.0 2,434,694 + Pf1N1B4_2123 0.39 -0.2 2,434,694 + -2.0 2,434,694 + Pf1N1B4_2123 0.39 -0.9 2,434,694 + Pf1N1B4_2123 0.39 +0.3 2,434,694 + Pf1N1B4_2123 0.39 -1.6 2,434,695 - Pf1N1B4_2123 0.39 -1.4 2,434,729 + Pf1N1B4_2123 0.56 -0.4 2,434,729 + Pf1N1B4_2123 0.56 -1.3 2,434,729 + Pf1N1B4_2123 0.56 -2.5 2,434,729 + Pf1N1B4_2123 0.56 +0.7 2,434,730 - Pf1N1B4_2123 0.57 -1.3 2,434,730 - Pf1N1B4_2123 0.57 -1.3 2,434,790 + Pf1N1B4_2123 0.87 -1.3 2,434,791 - Pf1N1B4_2123 0.87 -1.7 2,434,828 - +0.3 2,435,345 - -1.5 2,435,345 - -2.0 2,435,345 - +0.1
Or see this region's nucleotide sequence