Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1991 and Pf1N1B4_1992 overlap by 4 nucleotides Pf1N1B4_1992 and Pf1N1B4_1993 are separated by 127 nucleotides Pf1N1B4_1993 and Pf1N1B4_1994 are separated by 28 nucleotides
Pf1N1B4_1991 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 2,276,193 to 2,277,401
_1991
Pf1N1B4_1992 - Glutamine synthetase adenylyltransferase, at 2,277,398 to 2,277,775
_1992
Pf1N1B4_1993 - C4-type zinc finger protein, DksA/TraR family, at 2,277,903 to 2,278,307
_1993
Pf1N1B4_1994 - Putative metal chaperone, involved in Zn homeostasis, GTPase of COG0523 family, at 2,278,336 to 2,279,544
_1994
Position (kb)
2277
2278 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2276.426 kb on - strand, within Pf1N1B4_1991 at 2276.438 kb on + strand, within Pf1N1B4_1991 at 2276.439 kb on - strand, within Pf1N1B4_1991 at 2276.941 kb on - strand, within Pf1N1B4_1991 at 2277.196 kb on + strand, within Pf1N1B4_1991 at 2277.360 kb on + strand at 2277.360 kb on + strand at 2277.360 kb on + strand at 2277.361 kb on - strand at 2277.361 kb on - strand at 2277.493 kb on - strand, within Pf1N1B4_1992 at 2277.493 kb on - strand, within Pf1N1B4_1992 at 2277.588 kb on - strand, within Pf1N1B4_1992 at 2277.606 kb on + strand, within Pf1N1B4_1992 at 2277.606 kb on + strand, within Pf1N1B4_1992 at 2277.606 kb on + strand, within Pf1N1B4_1992 at 2277.606 kb on + strand, within Pf1N1B4_1992 at 2277.606 kb on + strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.636 kb on + strand, within Pf1N1B4_1992 at 2277.636 kb on + strand, within Pf1N1B4_1992 at 2277.656 kb on + strand, within Pf1N1B4_1992 at 2277.656 kb on + strand, within Pf1N1B4_1992 at 2277.656 kb on + strand, within Pf1N1B4_1992 at 2277.657 kb on - strand, within Pf1N1B4_1992 at 2277.657 kb on - strand, within Pf1N1B4_1992 at 2277.657 kb on - strand, within Pf1N1B4_1992 at 2277.657 kb on - strand, within Pf1N1B4_1992 at 2277.657 kb on - strand, within Pf1N1B4_1992 at 2277.802 kb on + strand at 2277.802 kb on + strand at 2277.803 kb on - strand at 2277.803 kb on - strand at 2277.828 kb on + strand at 2277.829 kb on - strand at 2277.942 kb on + strand at 2277.942 kb on + strand at 2277.943 kb on - strand at 2277.943 kb on - strand at 2277.943 kb on - strand at 2277.943 kb on - strand at 2278.253 kb on + strand, within Pf1N1B4_1993 at 2278.253 kb on + strand, within Pf1N1B4_1993 at 2278.330 kb on + strand at 2278.331 kb on - strand at 2278.331 kb on - strand at 2278.393 kb on - strand at 2278.401 kb on + strand at 2278.401 kb on + strand at 2278.402 kb on - strand at 2278.405 kb on + strand at 2278.405 kb on + strand at 2278.405 kb on + strand at 2278.405 kb on + strand at 2278.410 kb on - strand at 2278.413 kb on - strand at 2278.413 kb on - strand at 2278.712 kb on + strand, within Pf1N1B4_1994 at 2278.712 kb on + strand, within Pf1N1B4_1994 at 2278.712 kb on + strand, within Pf1N1B4_1994 at 2278.713 kb on - strand, within Pf1N1B4_1994 at 2278.721 kb on + strand, within Pf1N1B4_1994 at 2278.769 kb on - strand, within Pf1N1B4_1994 at 2278.769 kb on - strand, within Pf1N1B4_1994
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,276,426 - Pf1N1B4_1991 0.19 +1.1 2,276,438 + Pf1N1B4_1991 0.20 +0.6 2,276,439 - Pf1N1B4_1991 0.20 +0.4 2,276,941 - Pf1N1B4_1991 0.62 -1.2 2,277,196 + Pf1N1B4_1991 0.83 +0.4 2,277,360 + -0.5 2,277,360 + +0.4 2,277,360 + -1.2 2,277,361 - -2.1 2,277,361 - +0.6 2,277,493 - Pf1N1B4_1992 0.25 +0.4 2,277,493 - Pf1N1B4_1992 0.25 +0.0 2,277,588 - Pf1N1B4_1992 0.50 -0.6 2,277,606 + Pf1N1B4_1992 0.55 -0.2 2,277,606 + Pf1N1B4_1992 0.55 -0.6 2,277,606 + Pf1N1B4_1992 0.55 +0.1 2,277,606 + Pf1N1B4_1992 0.55 -0.6 2,277,606 + Pf1N1B4_1992 0.55 +0.9 2,277,607 - Pf1N1B4_1992 0.55 +0.4 2,277,607 - Pf1N1B4_1992 0.55 +0.8 2,277,607 - Pf1N1B4_1992 0.55 -0.9 2,277,607 - Pf1N1B4_1992 0.55 +0.4 2,277,607 - Pf1N1B4_1992 0.55 -0.2 2,277,607 - Pf1N1B4_1992 0.55 -0.2 2,277,607 - Pf1N1B4_1992 0.55 -1.1 2,277,607 - Pf1N1B4_1992 0.55 -1.2 2,277,607 - Pf1N1B4_1992 0.55 +0.4 2,277,607 - Pf1N1B4_1992 0.55 -0.8 2,277,636 + Pf1N1B4_1992 0.63 -0.8 2,277,636 + Pf1N1B4_1992 0.63 -0.6 2,277,656 + Pf1N1B4_1992 0.68 -1.0 2,277,656 + Pf1N1B4_1992 0.68 +0.4 2,277,656 + Pf1N1B4_1992 0.68 +0.9 2,277,657 - Pf1N1B4_1992 0.69 +0.4 2,277,657 - Pf1N1B4_1992 0.69 -1.4 2,277,657 - Pf1N1B4_1992 0.69 -0.6 2,277,657 - Pf1N1B4_1992 0.69 -1.6 2,277,657 - Pf1N1B4_1992 0.69 -1.0 2,277,802 + -1.2 2,277,802 + +1.9 2,277,803 - -1.4 2,277,803 - +0.4 2,277,828 + -1.8 2,277,829 - -0.6 2,277,942 + -0.4 2,277,942 + +0.4 2,277,943 - -2.0 2,277,943 - -1.6 2,277,943 - -0.6 2,277,943 - -1.2 2,278,253 + Pf1N1B4_1993 0.86 +0.9 2,278,253 + Pf1N1B4_1993 0.86 +0.7 2,278,330 + +0.0 2,278,331 - -1.2 2,278,331 - -1.6 2,278,393 - -0.6 2,278,401 + -2.0 2,278,401 + +0.1 2,278,402 - -0.4 2,278,405 + -0.2 2,278,405 + -0.4 2,278,405 + +0.4 2,278,405 + +1.4 2,278,410 - +0.4 2,278,413 - +0.4 2,278,413 - +1.7 2,278,712 + Pf1N1B4_1994 0.31 -0.6 2,278,712 + Pf1N1B4_1994 0.31 -0.3 2,278,712 + Pf1N1B4_1994 0.31 -1.0 2,278,713 - Pf1N1B4_1994 0.31 -1.0 2,278,721 + Pf1N1B4_1994 0.32 -0.6 2,278,769 - Pf1N1B4_1994 0.36 -1.6 2,278,769 - Pf1N1B4_1994 0.36 -1.2
Or see this region's nucleotide sequence