Experiment: L-Isoleucine (N) 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf1N1B4_1433 and Pf1N1B4_1434 are separated by 129 nucleotides Pf1N1B4_1434 and Pf1N1B4_1435 are separated by 135 nucleotides 
        Pf1N1B4_1433 - DnaK-related protein, at 1,641,810 to 1,643,600 
        _1433 
         
        
        Pf1N1B4_1434 - Phosphoserine phosphatase (EC 3.1.3.3), at 1,643,730 to 1,644,944 
        _1434 
         
        
        Pf1N1B4_1435 - HAMP domain protein, at 1,645,080 to 1,646,606 
        _1435 
         Position (kb) 1643 
1644 
1645 Strain fitness (log2 ratio) -4 
-3 
-2 
-1 
0 
1 
2 
3 at 1642.842 kb on + strand, within Pf1N1B4_1433 at 1642.843 kb on - strand at 1642.998 kb on + strand, within Pf1N1B4_1433 at 1643.076 kb on + strand, within Pf1N1B4_1433 at 1643.076 kb on + strand, within Pf1N1B4_1433 at 1643.076 kb on + strand, within Pf1N1B4_1433 at 1643.077 kb on - strand, within Pf1N1B4_1433 at 1643.077 kb on - strand, within Pf1N1B4_1433 at 1643.149 kb on + strand, within Pf1N1B4_1433 at 1643.328 kb on + strand, within Pf1N1B4_1433 at 1643.417 kb on + strand, within Pf1N1B4_1433 at 1643.417 kb on + strand, within Pf1N1B4_1433 at 1643.417 kb on + strand, within Pf1N1B4_1433 at 1643.418 kb on - strand, within Pf1N1B4_1433 at 1643.418 kb on - strand, within Pf1N1B4_1433 at 1643.433 kb on - strand at 1643.433 kb on - strand at 1643.480 kb on + strand at 1643.492 kb on + strand at 1643.492 kb on + strand at 1643.604 kb on + strand at 1643.604 kb on + strand at 1643.604 kb on + strand at 1643.604 kb on + strand at 1643.604 kb on + strand at 1643.605 kb on - strand at 1643.605 kb on - strand at 1643.605 kb on - strand at 1643.605 kb on - strand at 1643.605 kb on - strand at 1643.612 kb on + strand at 1643.613 kb on - strand at 1643.661 kb on + strand at 1643.661 kb on + strand at 1643.661 kb on + strand at 1643.662 kb on - strand at 1643.662 kb on - strand at 1643.662 kb on - strand at 1643.662 kb on - strand at 1643.662 kb on - strand at 1643.722 kb on + strand at 1643.723 kb on - strand at 1643.768 kb on - strand at 1643.845 kb on - strand at 1643.930 kb on + strand, within Pf1N1B4_1434 at 1643.930 kb on + strand, within Pf1N1B4_1434 at 1643.930 kb on + strand, within Pf1N1B4_1434 at 1643.931 kb on - strand, within Pf1N1B4_1434 at 1644.036 kb on + strand, within Pf1N1B4_1434 at 1644.036 kb on + strand, within Pf1N1B4_1434 at 1644.037 kb on - strand, within Pf1N1B4_1434 at 1644.037 kb on - strand, within Pf1N1B4_1434 at 1644.037 kb on - strand, within Pf1N1B4_1434 at 1644.037 kb on - strand, within Pf1N1B4_1434 at 1644.037 kb on - strand, within Pf1N1B4_1434 at 1644.037 kb on - strand, within Pf1N1B4_1434 at 1644.037 kb on - strand, within Pf1N1B4_1434 at 1644.037 kb on - strand, within Pf1N1B4_1434 at 1644.037 kb on - strand, within Pf1N1B4_1434 at 1644.037 kb on - strand, within Pf1N1B4_1434 at 1644.037 kb on - strand, within Pf1N1B4_1434 at 1644.037 kb on - strand, within Pf1N1B4_1434 at 1644.037 kb on - strand, within Pf1N1B4_1434 at 1644.040 kb on + strand, within Pf1N1B4_1434 at 1644.040 kb on + strand, within Pf1N1B4_1434 at 1644.040 kb on + strand, within Pf1N1B4_1434 at 1644.041 kb on - strand, within Pf1N1B4_1434 at 1644.041 kb on - strand, within Pf1N1B4_1434 at 1644.079 kb on + strand, within Pf1N1B4_1434 at 1644.561 kb on - strand, within Pf1N1B4_1434 at 1644.561 kb on - strand, within Pf1N1B4_1434 at 1644.561 kb on - strand, within Pf1N1B4_1434 at 1644.581 kb on - strand, within Pf1N1B4_1434 at 1644.581 kb on - strand, within Pf1N1B4_1434 at 1644.644 kb on + strand, within Pf1N1B4_1434 at 1644.737 kb on - strand, within Pf1N1B4_1434 at 1644.770 kb on + strand, within Pf1N1B4_1434 at 1644.771 kb on - strand, within Pf1N1B4_1434  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction L-Isoleucine (N) remove 1,642,842 +  Pf1N1B4_1433 0.58  +0.9 1,642,843 -  +0.8 1,642,998 +  Pf1N1B4_1433 0.66  -0.5 1,643,076 +  Pf1N1B4_1433 0.71  -0.9 1,643,076 +  Pf1N1B4_1433 0.71  -2.1 1,643,076 +  Pf1N1B4_1433 0.71  -1.1 1,643,077 -  Pf1N1B4_1433 0.71  -0.3 1,643,077 -  Pf1N1B4_1433 0.71  +0.2 1,643,149 +  Pf1N1B4_1433 0.75  +0.6 1,643,328 +  Pf1N1B4_1433 0.85  +1.2 1,643,417 +  Pf1N1B4_1433 0.90  +0.6 1,643,417 +  Pf1N1B4_1433 0.90  +3.2 1,643,417 +  Pf1N1B4_1433 0.90  -0.1 1,643,418 -  Pf1N1B4_1433 0.90  -0.8 1,643,418 -  Pf1N1B4_1433 0.90  +1.2 1,643,433 -  -2.1 1,643,433 -  -0.4 1,643,480 +  -1.0 1,643,492 +  -0.2 1,643,492 +  +0.2 1,643,604 +  +0.2 1,643,604 +  +1.0 1,643,604 +  +1.8 1,643,604 +  -0.8 1,643,604 +  +0.5 1,643,605 -  -1.6 1,643,605 -  -0.2 1,643,605 -  +0.2 1,643,605 -  +0.6 1,643,605 -  +0.5 1,643,612 +  -0.5 1,643,613 -  -0.4 1,643,661 +  -0.2 1,643,661 +  +0.2 1,643,661 +  -1.4 1,643,662 -  -1.4 1,643,662 -  +0.1 1,643,662 -  -0.4 1,643,662 -  -0.6 1,643,662 -  +0.2 1,643,722 +  -0.6 1,643,723 -  +0.2 1,643,768 -  -1.4 1,643,845 -  -0.8 1,643,930 +  Pf1N1B4_1434 0.16  -2.9 1,643,930 +  Pf1N1B4_1434 0.16  -3.9 1,643,930 +  Pf1N1B4_1434 0.16  -1.8 1,643,931 -  Pf1N1B4_1434 0.17  -2.6 1,644,036 +  Pf1N1B4_1434 0.25  -2.8 1,644,036 +  Pf1N1B4_1434 0.25  -0.6 1,644,037 -  Pf1N1B4_1434 0.25  -0.8 1,644,037 -  Pf1N1B4_1434 0.25  -0.8 1,644,037 -  Pf1N1B4_1434 0.25  -1.4 1,644,037 -  Pf1N1B4_1434 0.25  -0.8 1,644,037 -  Pf1N1B4_1434 0.25  -4.0 1,644,037 -  Pf1N1B4_1434 0.25  -1.4 1,644,037 -  Pf1N1B4_1434 0.25  +0.2 1,644,037 -  Pf1N1B4_1434 0.25  -3.9 1,644,037 -  Pf1N1B4_1434 0.25  -0.8 1,644,037 -  Pf1N1B4_1434 0.25  -1.8 1,644,037 -  Pf1N1B4_1434 0.25  -0.4 1,644,037 -  Pf1N1B4_1434 0.25  -1.8 1,644,037 -  Pf1N1B4_1434 0.25  -1.8 1,644,040 +  Pf1N1B4_1434 0.26  +0.2 1,644,040 +  Pf1N1B4_1434 0.26  -1.4 1,644,040 +  Pf1N1B4_1434 0.26  -0.8 1,644,041 -  Pf1N1B4_1434 0.26  -2.1 1,644,041 -  Pf1N1B4_1434 0.26  +0.2 1,644,079 +  Pf1N1B4_1434 0.29  -2.1 1,644,561 -  Pf1N1B4_1434 0.68  +0.2 1,644,561 -  Pf1N1B4_1434 0.68  -3.9 1,644,561 -  Pf1N1B4_1434 0.68  -1.8 1,644,581 -  Pf1N1B4_1434 0.70  -3.1 1,644,581 -  Pf1N1B4_1434 0.70  -1.8 1,644,644 +  Pf1N1B4_1434 0.75  -3.8 1,644,737 -  Pf1N1B4_1434 0.83  -3.5 1,644,770 +  Pf1N1B4_1434 0.86  -1.4 1,644,771 -  Pf1N1B4_1434 0.86  -2.8 
Or see this region's nucleotide sequence