Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_05320

Experiment: D-Glucose 20 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_05319 and H281DRAFT_05320 are separated by 233 nucleotidesH281DRAFT_05320 and H281DRAFT_05321 are separated by 125 nucleotidesH281DRAFT_05321 and H281DRAFT_05322 overlap by 4 nucleotides H281DRAFT_05319: H281DRAFT_05319 - 5-dehydro-4-deoxyglucarate dehydratase, at 69,905 to 70,822 _05319 H281DRAFT_05320: H281DRAFT_05320 - galactarate dehydratase, at 71,056 to 72,645 _05320 H281DRAFT_05321: H281DRAFT_05321 - uronate dehydrogenase, at 72,771 to 73,613 _05321 H281DRAFT_05322: H281DRAFT_05322 - gluconolactonase (EC 3.1.1.17), at 73,610 to 74,563 _05322 Position (kb) 71 72 73Strain fitness (log2 ratio) -2 -1 0 1 2at 70.173 kb on - strand, within H281DRAFT_05319at 70.209 kb on + strand, within H281DRAFT_05319at 70.210 kb on - strand, within H281DRAFT_05319at 70.395 kb on + strand, within H281DRAFT_05319at 70.395 kb on + strand, within H281DRAFT_05319at 70.396 kb on - strand, within H281DRAFT_05319at 70.479 kb on + strand, within H281DRAFT_05319at 70.479 kb on + strand, within H281DRAFT_05319at 70.480 kb on - strand, within H281DRAFT_05319at 70.639 kb on + strand, within H281DRAFT_05319at 70.640 kb on - strand, within H281DRAFT_05319at 70.722 kb on + strand, within H281DRAFT_05319at 70.816 kb on + strandat 70.816 kb on + strandat 70.817 kb on - strandat 70.827 kb on + strandat 70.827 kb on + strandat 70.827 kb on + strandat 70.827 kb on + strandat 70.827 kb on + strandat 70.827 kb on + strandat 70.827 kb on + strandat 70.828 kb on - strandat 70.828 kb on - strandat 70.828 kb on - strandat 70.828 kb on - strandat 70.828 kb on - strandat 70.828 kb on - strandat 70.828 kb on - strandat 70.828 kb on - strandat 70.832 kb on - strandat 70.846 kb on - strandat 70.947 kb on + strandat 70.948 kb on - strandat 70.948 kb on - strandat 70.948 kb on - strandat 70.968 kb on + strandat 70.968 kb on + strandat 70.968 kb on + strandat 70.968 kb on + strandat 70.968 kb on + strandat 70.969 kb on - strandat 71.074 kb on + strandat 71.237 kb on - strand, within H281DRAFT_05320at 71.238 kb on + strand, within H281DRAFT_05320at 71.238 kb on + strand, within H281DRAFT_05320at 71.560 kb on + strand, within H281DRAFT_05320at 71.560 kb on + strand, within H281DRAFT_05320at 71.560 kb on + strand, within H281DRAFT_05320at 71.560 kb on + strand, within H281DRAFT_05320at 71.561 kb on - strand, within H281DRAFT_05320at 71.561 kb on - strand, within H281DRAFT_05320at 71.561 kb on - strand, within H281DRAFT_05320at 71.623 kb on - strand, within H281DRAFT_05320at 72.069 kb on - strand, within H281DRAFT_05320at 72.128 kb on - strand, within H281DRAFT_05320at 72.247 kb on - strand, within H281DRAFT_05320at 72.307 kb on + strand, within H281DRAFT_05320at 72.307 kb on + strand, within H281DRAFT_05320at 72.333 kb on + strand, within H281DRAFT_05320at 72.405 kb on + strand, within H281DRAFT_05320at 72.406 kb on - strand, within H281DRAFT_05320at 72.684 kb on + strandat 72.685 kb on - strandat 72.720 kb on + strandat 72.720 kb on + strandat 72.721 kb on - strandat 72.751 kb on + strandat 72.751 kb on + strandat 72.761 kb on + strandat 73.089 kb on + strand, within H281DRAFT_05321at 73.251 kb on + strand, within H281DRAFT_05321at 73.254 kb on + strand, within H281DRAFT_05321at 73.254 kb on + strand, within H281DRAFT_05321at 73.255 kb on - strand, within H281DRAFT_05321at 73.255 kb on - strand, within H281DRAFT_05321at 73.350 kb on + strand, within H281DRAFT_05321at 73.417 kb on - strand, within H281DRAFT_05321at 73.582 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose 20 mM (C)
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70,173 - H281DRAFT_05319 0.29 +1.8
70,209 + H281DRAFT_05319 0.33 -0.2
70,210 - H281DRAFT_05319 0.33 +0.4
70,395 + H281DRAFT_05319 0.53 +0.3
70,395 + H281DRAFT_05319 0.53 +0.4
70,396 - H281DRAFT_05319 0.53 -0.4
70,479 + H281DRAFT_05319 0.63 +0.6
70,479 + H281DRAFT_05319 0.63 -0.9
70,480 - H281DRAFT_05319 0.63 -0.4
70,639 + H281DRAFT_05319 0.80 -0.7
70,640 - H281DRAFT_05319 0.80 +0.4
70,722 + H281DRAFT_05319 0.89 -0.8
70,816 + -0.3
70,816 + -0.4
70,817 - +1.4
70,827 + +0.3
70,827 + +0.2
70,827 + +0.2
70,827 + -0.1
70,827 + -0.5
70,827 + +0.4
70,827 + +0.3
70,828 - +0.6
70,828 - +0.5
70,828 - -0.7
70,828 - +0.8
70,828 - -0.0
70,828 - +0.3
70,828 - -0.3
70,828 - -0.2
70,832 - +0.0
70,846 - -1.5
70,947 + -0.1
70,948 - +0.2
70,948 - -0.3
70,948 - +0.7
70,968 + +1.0
70,968 + +0.6
70,968 + +1.1
70,968 + -0.1
70,968 + -0.2
70,969 - +0.1
71,074 + -0.8
71,237 - H281DRAFT_05320 0.11 +2.1
71,238 + H281DRAFT_05320 0.11 -0.9
71,238 + H281DRAFT_05320 0.11 -0.8
71,560 + H281DRAFT_05320 0.32 +0.6
71,560 + H281DRAFT_05320 0.32 +0.9
71,560 + H281DRAFT_05320 0.32 -0.1
71,560 + H281DRAFT_05320 0.32 +0.2
71,561 - H281DRAFT_05320 0.32 +1.4
71,561 - H281DRAFT_05320 0.32 +0.1
71,561 - H281DRAFT_05320 0.32 -0.5
71,623 - H281DRAFT_05320 0.36 +0.0
72,069 - H281DRAFT_05320 0.64 +1.2
72,128 - H281DRAFT_05320 0.67 -0.4
72,247 - H281DRAFT_05320 0.75 +2.5
72,307 + H281DRAFT_05320 0.79 -1.8
72,307 + H281DRAFT_05320 0.79 +0.2
72,333 + H281DRAFT_05320 0.80 +0.6
72,405 + H281DRAFT_05320 0.85 +0.4
72,406 - H281DRAFT_05320 0.85 -0.5
72,684 + +0.5
72,685 - +0.4
72,720 + +0.5
72,720 + -1.9
72,721 - +0.0
72,751 + -0.2
72,751 + +0.1
72,761 + +0.2
73,089 + H281DRAFT_05321 0.38 +1.4
73,251 + H281DRAFT_05321 0.57 -1.1
73,254 + H281DRAFT_05321 0.57 +0.9
73,254 + H281DRAFT_05321 0.57 +0.4
73,255 - H281DRAFT_05321 0.57 +0.5
73,255 - H281DRAFT_05321 0.57 +1.2
73,350 + H281DRAFT_05321 0.69 +0.8
73,417 - H281DRAFT_05321 0.77 +0.3
73,582 + +0.1

Or see this region's nucleotide sequence