Experiment: incubated with with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate for 3 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3516 and Dsui_3517 overlap by 4 nucleotides Dsui_3517 and Dsui_3518 are separated by 84 nucleotides Dsui_3518 and Dsui_3519 are separated by 72 nucleotides
Dsui_3516: Dsui_3516 - hypothetical protein, at 3,779,723 to 3,781,249
_3516
Dsui_3517: Dsui_3517 - putative membrane protein, at 3,781,246 to 3,782,076
_3517
Dsui_3518: Dsui_3518 - putative membrane protein, at 3,782,161 to 3,782,523
_3518
Dsui_3519: Dsui_3519 - 2-isopropylmalate synthase, bacterial type, at 3,782,596 to 3,784,137
_3519
Position (kb)
3781
3782
3783 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3780.294 kb on + strand, within Dsui_3516 at 3780.310 kb on - strand at 3780.310 kb on - strand, within Dsui_3516 at 3780.331 kb on - strand, within Dsui_3516 at 3780.368 kb on - strand, within Dsui_3516 at 3780.427 kb on + strand, within Dsui_3516 at 3780.446 kb on - strand, within Dsui_3516 at 3780.531 kb on - strand, within Dsui_3516 at 3780.585 kb on + strand, within Dsui_3516 at 3780.619 kb on - strand, within Dsui_3516 at 3780.810 kb on + strand, within Dsui_3516 at 3780.818 kb on - strand, within Dsui_3516 at 3780.846 kb on + strand, within Dsui_3516 at 3780.847 kb on + strand, within Dsui_3516 at 3780.854 kb on - strand, within Dsui_3516 at 3780.855 kb on - strand, within Dsui_3516 at 3780.913 kb on + strand, within Dsui_3516 at 3780.915 kb on + strand, within Dsui_3516 at 3780.963 kb on - strand, within Dsui_3516 at 3781.179 kb on + strand at 3781.187 kb on - strand at 3781.207 kb on + strand at 3781.221 kb on - strand at 3781.315 kb on + strand at 3781.327 kb on - strand at 3781.371 kb on + strand, within Dsui_3517 at 3781.379 kb on - strand, within Dsui_3517 at 3781.386 kb on - strand, within Dsui_3517 at 3781.575 kb on + strand, within Dsui_3517 at 3781.605 kb on + strand, within Dsui_3517 at 3781.605 kb on + strand, within Dsui_3517 at 3781.643 kb on + strand, within Dsui_3517 at 3781.728 kb on - strand, within Dsui_3517 at 3781.750 kb on + strand, within Dsui_3517 at 3781.773 kb on + strand, within Dsui_3517 at 3781.773 kb on + strand, within Dsui_3517 at 3781.820 kb on + strand, within Dsui_3517 at 3781.823 kb on - strand, within Dsui_3517 at 3781.829 kb on - strand, within Dsui_3517 at 3781.879 kb on + strand at 3781.880 kb on + strand at 3781.880 kb on + strand, within Dsui_3517 at 3781.880 kb on + strand at 3781.880 kb on + strand, within Dsui_3517 at 3781.888 kb on - strand, within Dsui_3517 at 3781.888 kb on - strand, within Dsui_3517 at 3781.888 kb on - strand, within Dsui_3517 at 3781.888 kb on - strand, within Dsui_3517 at 3781.965 kb on + strand, within Dsui_3517 at 3781.973 kb on + strand, within Dsui_3517 at 3781.973 kb on + strand, within Dsui_3517 at 3781.981 kb on - strand, within Dsui_3517 at 3781.981 kb on - strand, within Dsui_3517 at 3782.015 kb on - strand at 3782.065 kb on - strand at 3782.065 kb on - strand at 3782.065 kb on - strand at 3782.109 kb on + strand at 3782.109 kb on + strand at 3782.122 kb on - strand at 3782.171 kb on + strand at 3782.232 kb on - strand, within Dsui_3518 at 3782.269 kb on - strand, within Dsui_3518 at 3782.325 kb on + strand, within Dsui_3518 at 3782.333 kb on - strand, within Dsui_3518 at 3782.333 kb on - strand, within Dsui_3518 at 3782.369 kb on - strand, within Dsui_3518 at 3782.393 kb on + strand, within Dsui_3518 at 3782.401 kb on - strand, within Dsui_3518 at 3782.404 kb on + strand, within Dsui_3518 at 3782.465 kb on - strand, within Dsui_3518 at 3782.525 kb on + strand at 3782.525 kb on + strand at 3782.526 kb on - strand at 3782.605 kb on + strand at 3782.605 kb on + strand at 3782.630 kb on + strand at 3782.662 kb on + strand at 3782.670 kb on - strand at 3782.671 kb on - strand at 3782.731 kb on - strand at 3782.831 kb on + strand, within Dsui_3519 at 3782.839 kb on + strand, within Dsui_3519 at 3782.839 kb on - strand, within Dsui_3519 at 3782.847 kb on - strand, within Dsui_3519 at 3782.944 kb on - strand, within Dsui_3519 at 3782.976 kb on - strand, within Dsui_3519 at 3783.007 kb on + strand, within Dsui_3519
Per-strain Table
Position Strand Gene LocusTag Fraction incubated with with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate for 3 hours remove 3,780,294 + Dsui_3516 0.37 +0.1 3,780,310 - -0.6 3,780,310 - Dsui_3516 0.38 +0.4 3,780,331 - Dsui_3516 0.40 -1.0 3,780,368 - Dsui_3516 0.42 -0.5 3,780,427 + Dsui_3516 0.46 +2.3 3,780,446 - Dsui_3516 0.47 -1.2 3,780,531 - Dsui_3516 0.53 -0.3 3,780,585 + Dsui_3516 0.56 +0.9 3,780,619 - Dsui_3516 0.59 -0.8 3,780,810 + Dsui_3516 0.71 +0.7 3,780,818 - Dsui_3516 0.72 +0.2 3,780,846 + Dsui_3516 0.74 +0.9 3,780,847 + Dsui_3516 0.74 +1.1 3,780,854 - Dsui_3516 0.74 +0.4 3,780,855 - Dsui_3516 0.74 +0.5 3,780,913 + Dsui_3516 0.78 +1.2 3,780,915 + Dsui_3516 0.78 +1.7 3,780,963 - Dsui_3516 0.81 +0.4 3,781,179 + -0.4 3,781,187 - +0.8 3,781,207 + +1.1 3,781,221 - +1.2 3,781,315 + +0.5 3,781,327 - +0.5 3,781,371 + Dsui_3517 0.15 +0.5 3,781,379 - Dsui_3517 0.16 +1.5 3,781,386 - Dsui_3517 0.17 +0.3 3,781,575 + Dsui_3517 0.40 -2.4 3,781,605 + Dsui_3517 0.43 +0.4 3,781,605 + Dsui_3517 0.43 +1.8 3,781,643 + Dsui_3517 0.48 +0.8 3,781,728 - Dsui_3517 0.58 +0.5 3,781,750 + Dsui_3517 0.61 +0.4 3,781,773 + Dsui_3517 0.63 -0.7 3,781,773 + Dsui_3517 0.63 -1.0 3,781,820 + Dsui_3517 0.69 -2.3 3,781,823 - Dsui_3517 0.69 +0.7 3,781,829 - Dsui_3517 0.70 -1.5 3,781,879 + -2.4 3,781,880 + -2.3 3,781,880 + Dsui_3517 0.76 -2.2 3,781,880 + +0.7 3,781,880 + Dsui_3517 0.76 -1.2 3,781,888 - Dsui_3517 0.77 -0.4 3,781,888 - Dsui_3517 0.77 +0.8 3,781,888 - Dsui_3517 0.77 +0.4 3,781,888 - Dsui_3517 0.77 +1.4 3,781,965 + Dsui_3517 0.87 -1.0 3,781,973 + Dsui_3517 0.87 -0.5 3,781,973 + Dsui_3517 0.87 -2.3 3,781,981 - Dsui_3517 0.88 +0.6 3,781,981 - Dsui_3517 0.88 +0.9 3,782,015 - +0.8 3,782,065 - -0.0 3,782,065 - +0.6 3,782,065 - +1.9 3,782,109 + +0.0 3,782,109 + -3.8 3,782,122 - -0.1 3,782,171 + -0.5 3,782,232 - Dsui_3518 0.20 +0.6 3,782,269 - Dsui_3518 0.30 +1.7 3,782,325 + Dsui_3518 0.45 -0.5 3,782,333 - Dsui_3518 0.47 +0.4 3,782,333 - Dsui_3518 0.47 -0.5 3,782,369 - Dsui_3518 0.57 +0.5 3,782,393 + Dsui_3518 0.64 +0.0 3,782,401 - Dsui_3518 0.66 +0.6 3,782,404 + Dsui_3518 0.67 -0.4 3,782,465 - Dsui_3518 0.84 +0.4 3,782,525 + +1.0 3,782,525 + +0.4 3,782,526 - +0.8 3,782,605 + +0.0 3,782,605 + -1.4 3,782,630 + +1.1 3,782,662 + -0.1 3,782,670 - +1.0 3,782,671 - +1.2 3,782,731 - +1.2 3,782,831 + Dsui_3519 0.15 +0.4 3,782,839 + Dsui_3519 0.16 +0.7 3,782,839 - Dsui_3519 0.16 +0.7 3,782,847 - Dsui_3519 0.16 +0.3 3,782,944 - Dsui_3519 0.23 +1.2 3,782,976 - Dsui_3519 0.25 +1.0 3,783,007 + Dsui_3519 0.27 -2.1
Or see this region's nucleotide sequence