Experiment: incubated with with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate for 3 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3194 and Dsui_3195 overlap by 4 nucleotides Dsui_3195 and Dsui_3196 are separated by 130 nucleotides Dsui_3196 and Dsui_3197 are separated by 215 nucleotides
Dsui_3194: Dsui_3194 - pseudouridylate synthase I, at 3,428,225 to 3,429,052
_3194
Dsui_3195: Dsui_3195 - Cobalt transport protein, at 3,429,049 to 3,429,678
_3195
Dsui_3196: Dsui_3196 - FimV N-terminal domain protein, at 3,429,809 to 3,432,817
_3196
Dsui_3197: Dsui_3197 - aspartate-semialdehyde dehydrogenase, gamma-proteobacterial, at 3,433,033 to 3,434,166
_3197
Position (kb)
3429
3430
3431
3432
3433 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3428.992 kb on + strand at 3428.993 kb on + strand at 3429.012 kb on - strand at 3429.027 kb on - strand at 3429.027 kb on - strand at 3429.066 kb on - strand at 3429.290 kb on - strand, within Dsui_3195 at 3429.407 kb on - strand at 3429.431 kb on - strand at 3429.581 kb on + strand at 3429.826 kb on + strand at 3429.897 kb on - strand at 3430.022 kb on - strand at 3430.068 kb on + strand at 3430.377 kb on - strand, within Dsui_3196 at 3430.416 kb on + strand at 3430.417 kb on + strand, within Dsui_3196 at 3430.482 kb on + strand, within Dsui_3196 at 3430.556 kb on - strand, within Dsui_3196 at 3430.556 kb on - strand, within Dsui_3196 at 3430.604 kb on + strand, within Dsui_3196 at 3430.622 kb on + strand, within Dsui_3196 at 3430.625 kb on - strand, within Dsui_3196 at 3430.653 kb on + strand, within Dsui_3196 at 3430.661 kb on + strand, within Dsui_3196 at 3430.673 kb on - strand, within Dsui_3196 at 3430.960 kb on - strand, within Dsui_3196 at 3430.988 kb on - strand, within Dsui_3196 at 3430.998 kb on - strand, within Dsui_3196 at 3431.034 kb on - strand, within Dsui_3196 at 3431.052 kb on + strand, within Dsui_3196 at 3431.146 kb on + strand, within Dsui_3196 at 3431.357 kb on + strand, within Dsui_3196 at 3431.416 kb on + strand, within Dsui_3196 at 3431.558 kb on - strand, within Dsui_3196 at 3431.772 kb on + strand, within Dsui_3196 at 3431.772 kb on + strand, within Dsui_3196 at 3431.820 kb on + strand, within Dsui_3196 at 3431.827 kb on - strand, within Dsui_3196 at 3431.868 kb on - strand, within Dsui_3196 at 3431.937 kb on + strand, within Dsui_3196 at 3431.940 kb on - strand, within Dsui_3196 at 3431.970 kb on + strand, within Dsui_3196 at 3432.041 kb on - strand, within Dsui_3196 at 3432.119 kb on + strand, within Dsui_3196 at 3432.138 kb on + strand, within Dsui_3196 at 3432.143 kb on + strand, within Dsui_3196 at 3432.171 kb on - strand, within Dsui_3196 at 3432.177 kb on + strand at 3432.441 kb on + strand, within Dsui_3196 at 3432.442 kb on - strand, within Dsui_3196 at 3432.525 kb on + strand at 3432.538 kb on - strand at 3432.538 kb on - strand at 3432.574 kb on + strand at 3432.608 kb on + strand at 3432.657 kb on + strand at 3432.670 kb on - strand at 3432.766 kb on - strand at 3432.792 kb on - strand at 3432.831 kb on + strand at 3432.834 kb on + strand at 3432.885 kb on + strand at 3432.885 kb on + strand at 3432.885 kb on + strand at 3432.893 kb on - strand at 3432.893 kb on - strand at 3432.893 kb on - strand at 3432.893 kb on - strand at 3432.893 kb on - strand at 3432.893 kb on - strand at 3432.893 kb on - strand at 3432.893 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction incubated with with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate for 3 hours remove 3,428,992 + -1.7 3,428,993 + -2.3 3,429,012 - -3.2 3,429,027 - -1.4 3,429,027 - -2.8 3,429,066 - -0.1 3,429,290 - Dsui_3195 0.38 -1.6 3,429,407 - +1.3 3,429,431 - -1.0 3,429,581 + -2.9 3,429,826 + -1.2 3,429,897 - +0.4 3,430,022 - +0.5 3,430,068 + -0.3 3,430,377 - Dsui_3196 0.19 +0.9 3,430,416 + +2.5 3,430,417 + Dsui_3196 0.20 +0.0 3,430,482 + Dsui_3196 0.22 +0.3 3,430,556 - Dsui_3196 0.25 +1.1 3,430,556 - Dsui_3196 0.25 -0.1 3,430,604 + Dsui_3196 0.26 +0.6 3,430,622 + Dsui_3196 0.27 -0.3 3,430,625 - Dsui_3196 0.27 +0.4 3,430,653 + Dsui_3196 0.28 -0.0 3,430,661 + Dsui_3196 0.28 +0.3 3,430,673 - Dsui_3196 0.29 +1.0 3,430,960 - Dsui_3196 0.38 +0.3 3,430,988 - Dsui_3196 0.39 +1.0 3,430,998 - Dsui_3196 0.40 +1.1 3,431,034 - Dsui_3196 0.41 +1.0 3,431,052 + Dsui_3196 0.41 +0.9 3,431,146 + Dsui_3196 0.44 +0.6 3,431,357 + Dsui_3196 0.51 +0.3 3,431,416 + Dsui_3196 0.53 +1.0 3,431,558 - Dsui_3196 0.58 +1.1 3,431,772 + Dsui_3196 0.65 +0.1 3,431,772 + Dsui_3196 0.65 +0.4 3,431,820 + Dsui_3196 0.67 +0.6 3,431,827 - Dsui_3196 0.67 +0.9 3,431,868 - Dsui_3196 0.68 +0.9 3,431,937 + Dsui_3196 0.71 -0.5 3,431,940 - Dsui_3196 0.71 +1.5 3,431,970 + Dsui_3196 0.72 +0.4 3,432,041 - Dsui_3196 0.74 +0.4 3,432,119 + Dsui_3196 0.77 +1.6 3,432,138 + Dsui_3196 0.77 +0.1 3,432,143 + Dsui_3196 0.78 -2.6 3,432,171 - Dsui_3196 0.78 +0.8 3,432,177 + -1.8 3,432,441 + Dsui_3196 0.87 +0.0 3,432,442 - Dsui_3196 0.88 +1.4 3,432,525 + -0.7 3,432,538 - +0.7 3,432,538 - +1.6 3,432,574 + +1.1 3,432,608 + +0.1 3,432,657 + +0.5 3,432,670 - +0.8 3,432,766 - +1.2 3,432,792 - +0.6 3,432,831 + -0.1 3,432,834 + -3.1 3,432,885 + +0.7 3,432,885 + +0.3 3,432,885 + -0.2 3,432,893 - +1.3 3,432,893 - +1.1 3,432,893 - +1.2 3,432,893 - -0.2 3,432,893 - +0.0 3,432,893 - +1.1 3,432,893 - +0.8 3,432,893 - +0.7
Or see this region's nucleotide sequence