Experiment: incubated with with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate for 3 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0686 and Dsui_0687 overlap by 17 nucleotides Dsui_0687 and Dsui_0688 are separated by 95 nucleotides Dsui_0688 and Dsui_0689 overlap by 1 nucleotides Dsui_0689 and Dsui_0690 are separated by 72 nucleotides Dsui_0690 and Dsui_0691 are separated by 160 nucleotides Dsui_0691 and Dsui_0692 are separated by 17 nucleotides
Dsui_0686: Dsui_0686 - tRNA nucleotidyltransferase/poly(A) polymerase, at 684,017 to 685,237
_0686
Dsui_0687: Dsui_0687 - Protein of unknown function (DUF2905), at 685,221 to 685,412
_0687
Dsui_0688: Dsui_0688 - glutathione S-transferase, at 685,508 to 686,125
_0688
Dsui_0689: Dsui_0689 - lysophospholipase, at 686,125 to 686,937
_0689
Dsui_0690: Dsui_0690 - hypothetical protein, at 687,010 to 687,204
_0690
Dsui_0691: Dsui_0691 - hypothetical protein, at 687,365 to 687,562
_0691
Dsui_0692: Dsui_0692 - hypothetical protein, at 687,580 to 688,434
_0692
Position (kb) 686
687 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 685.268 kb on - strand, within Dsui_0687 at 685.274 kb on - strand, within Dsui_0687 at 685.318 kb on - strand, within Dsui_0687 at 685.379 kb on + strand, within Dsui_0687 at 685.387 kb on - strand, within Dsui_0687 at 685.422 kb on - strand at 685.493 kb on + strand at 685.501 kb on - strand at 685.513 kb on - strand at 685.514 kb on + strand at 685.525 kb on - strand at 685.574 kb on + strand, within Dsui_0688 at 685.579 kb on + strand, within Dsui_0688 at 685.579 kb on + strand, within Dsui_0688 at 685.579 kb on + strand, within Dsui_0688 at 685.579 kb on + strand, within Dsui_0688 at 685.587 kb on - strand at 685.587 kb on - strand, within Dsui_0688 at 685.587 kb on - strand, within Dsui_0688 at 685.587 kb on - strand, within Dsui_0688 at 685.587 kb on - strand, within Dsui_0688 at 685.587 kb on - strand at 685.616 kb on - strand at 685.616 kb on - strand, within Dsui_0688 at 685.641 kb on - strand, within Dsui_0688 at 685.701 kb on + strand, within Dsui_0688 at 685.776 kb on + strand, within Dsui_0688 at 685.877 kb on - strand, within Dsui_0688 at 685.921 kb on + strand, within Dsui_0688 at 685.921 kb on + strand, within Dsui_0688 at 685.940 kb on + strand, within Dsui_0688 at 685.952 kb on + strand, within Dsui_0688 at 685.952 kb on + strand, within Dsui_0688 at 686.001 kb on + strand, within Dsui_0688 at 686.106 kb on + strand at 686.114 kb on - strand at 686.114 kb on - strand at 686.190 kb on + strand at 686.329 kb on + strand at 686.329 kb on + strand, within Dsui_0689 at 686.435 kb on - strand, within Dsui_0689 at 686.446 kb on + strand, within Dsui_0689 at 686.485 kb on + strand, within Dsui_0689 at 686.559 kb on + strand, within Dsui_0689 at 686.588 kb on - strand, within Dsui_0689 at 686.797 kb on + strand, within Dsui_0689 at 686.905 kb on + strand at 686.925 kb on - strand at 686.998 kb on + strand at 687.016 kb on - strand at 687.028 kb on - strand at 687.051 kb on + strand, within Dsui_0690 at 687.059 kb on - strand, within Dsui_0690 at 687.069 kb on - strand, within Dsui_0690 at 687.095 kb on + strand, within Dsui_0690 at 687.110 kb on + strand, within Dsui_0690 at 687.129 kb on - strand, within Dsui_0690 at 687.144 kb on + strand at 687.159 kb on - strand, within Dsui_0690 at 687.214 kb on - strand at 687.288 kb on + strand at 687.294 kb on + strand at 687.311 kb on + strand at 687.311 kb on + strand at 687.311 kb on + strand at 687.311 kb on + strand at 687.311 kb on + strand at 687.319 kb on - strand at 687.319 kb on - strand at 687.342 kb on + strand at 687.348 kb on - strand at 687.386 kb on + strand, within Dsui_0691 at 687.466 kb on + strand, within Dsui_0691 at 687.539 kb on - strand, within Dsui_0691 at 687.556 kb on + strand at 687.581 kb on - strand at 687.647 kb on + strand at 687.655 kb on - strand at 687.716 kb on - strand, within Dsui_0692 at 687.748 kb on - strand at 687.756 kb on + strand, within Dsui_0692 at 687.786 kb on + strand, within Dsui_0692 at 687.856 kb on - strand, within Dsui_0692 at 687.873 kb on - strand, within Dsui_0692
Per-strain Table
Position Strand Gene LocusTag Fraction incubated with with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate for 3 hours remove 685,268 - Dsui_0687 0.24 +1.3 685,274 - Dsui_0687 0.28 +0.3 685,318 - Dsui_0687 0.51 +0.3 685,379 + Dsui_0687 0.82 +0.2 685,387 - Dsui_0687 0.86 +0.0 685,422 - +1.9 685,493 + +0.4 685,501 - +0.3 685,513 - +0.1 685,514 + +0.1 685,525 - +0.2 685,574 + Dsui_0688 0.11 +0.4 685,579 + Dsui_0688 0.11 -1.0 685,579 + Dsui_0688 0.11 -0.5 685,579 + Dsui_0688 0.11 +0.4 685,579 + Dsui_0688 0.11 +0.6 685,587 - -3.8 685,587 - Dsui_0688 0.13 -0.2 685,587 - Dsui_0688 0.13 -0.8 685,587 - Dsui_0688 0.13 +0.0 685,587 - Dsui_0688 0.13 +0.3 685,587 - -3.1 685,616 - -0.3 685,616 - Dsui_0688 0.17 +0.7 685,641 - Dsui_0688 0.22 +0.0 685,701 + Dsui_0688 0.31 +0.2 685,776 + Dsui_0688 0.43 +0.5 685,877 - Dsui_0688 0.60 -0.5 685,921 + Dsui_0688 0.67 +0.5 685,921 + Dsui_0688 0.67 +0.6 685,940 + Dsui_0688 0.70 -0.5 685,952 + Dsui_0688 0.72 +0.6 685,952 + Dsui_0688 0.72 +0.6 686,001 + Dsui_0688 0.80 +0.1 686,106 + +1.2 686,114 - +0.5 686,114 - -0.7 686,190 + -2.8 686,329 + -0.8 686,329 + Dsui_0689 0.25 +0.0 686,435 - Dsui_0689 0.38 +0.9 686,446 + Dsui_0689 0.39 -2.0 686,485 + Dsui_0689 0.44 -1.2 686,559 + Dsui_0689 0.53 -0.1 686,588 - Dsui_0689 0.57 -0.4 686,797 + Dsui_0689 0.83 -1.0 686,905 + -0.5 686,925 - +0.7 686,998 + -0.1 687,016 - -0.6 687,028 - +5.6 687,051 + Dsui_0690 0.21 +0.2 687,059 - Dsui_0690 0.25 +1.3 687,069 - Dsui_0690 0.30 +1.7 687,095 + Dsui_0690 0.44 -0.2 687,110 + Dsui_0690 0.51 -4.0 687,129 - Dsui_0690 0.61 +1.4 687,144 + -3.0 687,159 - Dsui_0690 0.76 +0.8 687,214 - +0.7 687,288 + +0.1 687,294 + +0.5 687,311 + -0.1 687,311 + +0.1 687,311 + -0.8 687,311 + +0.7 687,311 + +0.7 687,319 - -0.3 687,319 - +0.3 687,342 + +2.1 687,348 - +0.7 687,386 + Dsui_0691 0.11 +0.4 687,466 + Dsui_0691 0.51 +1.4 687,539 - Dsui_0691 0.88 +0.7 687,556 + +0.6 687,581 - +0.3 687,647 + +1.9 687,655 - +0.6 687,716 - Dsui_0692 0.16 -0.3 687,748 - -0.4 687,756 + Dsui_0692 0.21 -2.0 687,786 + Dsui_0692 0.24 +0.9 687,856 - Dsui_0692 0.32 +0.2 687,873 - Dsui_0692 0.34 -1.4
Or see this region's nucleotide sequence