Experiment: incubated with with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate for 3 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0046 and Dsui_0047 are separated by 111 nucleotides Dsui_0047 and Dsui_0048 are separated by 165 nucleotides
Dsui_0046: Dsui_0046 - Zn-dependent oligopeptidase, at 41,555 to 43,636
_0046
Dsui_0047: Dsui_0047 - putative integral membrane protein, at 43,748 to 44,518
_0047
Dsui_0048: Dsui_0048 - phosphoribosylaminoimidazole-succinocarboxamide synthase, at 44,684 to 45,622
_0048
Position (kb)
43
44
45 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 42.750 kb on + strand at 42.750 kb on + strand, within Dsui_0046 at 42.760 kb on + strand at 42.813 kb on - strand, within Dsui_0046 at 42.821 kb on + strand, within Dsui_0046 at 42.834 kb on - strand, within Dsui_0046 at 42.836 kb on + strand, within Dsui_0046 at 42.896 kb on + strand at 42.981 kb on + strand, within Dsui_0046 at 43.166 kb on - strand, within Dsui_0046 at 43.272 kb on - strand, within Dsui_0046 at 43.292 kb on - strand, within Dsui_0046 at 43.292 kb on - strand, within Dsui_0046 at 43.292 kb on - strand, within Dsui_0046 at 43.311 kb on - strand, within Dsui_0046 at 43.422 kb on + strand at 43.572 kb on - strand at 43.596 kb on - strand at 43.601 kb on - strand at 43.642 kb on + strand at 43.642 kb on + strand at 43.655 kb on - strand at 43.694 kb on + strand at 43.815 kb on - strand at 43.851 kb on - strand, within Dsui_0047 at 43.851 kb on - strand, within Dsui_0047 at 43.851 kb on - strand, within Dsui_0047 at 43.874 kb on - strand, within Dsui_0047 at 44.022 kb on - strand, within Dsui_0047 at 44.184 kb on - strand, within Dsui_0047 at 44.535 kb on + strand at 44.547 kb on + strand at 44.563 kb on + strand at 44.595 kb on + strand at 44.632 kb on - strand at 44.701 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction incubated with with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate for 3 hours remove 42,750 + -2.2 42,750 + Dsui_0046 0.57 -0.3 42,760 + -1.7 42,813 - Dsui_0046 0.60 -0.2 42,821 + Dsui_0046 0.61 -1.0 42,834 - Dsui_0046 0.61 -0.4 42,836 + Dsui_0046 0.62 +0.3 42,896 + -1.8 42,981 + Dsui_0046 0.68 -0.3 43,166 - Dsui_0046 0.77 -1.9 43,272 - Dsui_0046 0.82 +0.3 43,292 - Dsui_0046 0.83 +0.6 43,292 - Dsui_0046 0.83 -0.6 43,292 - Dsui_0046 0.83 +1.5 43,311 - Dsui_0046 0.84 -0.4 43,422 + +0.3 43,572 - +0.8 43,596 - -0.4 43,601 - -0.3 43,642 + -0.4 43,642 + +0.2 43,655 - +0.6 43,694 + +0.6 43,815 - +1.6 43,851 - Dsui_0047 0.13 +0.5 43,851 - Dsui_0047 0.13 +1.0 43,851 - Dsui_0047 0.13 +1.3 43,874 - Dsui_0047 0.16 +0.7 44,022 - Dsui_0047 0.36 +0.7 44,184 - Dsui_0047 0.57 +1.3 44,535 + +1.8 44,547 + +2.0 44,563 + -1.3 44,595 + -0.0 44,632 - +1.2 44,701 - +0.6
Or see this region's nucleotide sequence