Experiment: Ceftazidime 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rpl21 and rpl27 are separated by 34 nucleotides rpl27 and cheY are separated by 121 nucleotides cheY and truB are separated by 92 nucleotides truB and Synpcc7942_1223 are separated by 20 nucleotides
Synpcc7942_1219: rpl21 - 50S ribosomal protein L21, at 1,241,649 to 1,242,032
rpl21
Synpcc7942_1220: rpl27 - 50S ribosomal protein L27, at 1,242,067 to 1,242,333
rpl27
Synpcc7942_1221: cheY - response regulator receiver domain protein (CheY-like), at 1,242,455 to 1,242,832
cheY
Synpcc7942_1222: truB - tRNA pseudouridine synthase B, at 1,242,925 to 1,243,809
truB
Synpcc7942_1223: Synpcc7942_1223 - DevC protein, at 1,243,830 to 1,245,008
_1223
Position (kb)
1242
1243 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1241.480 kb on + strand at 1241.480 kb on + strand at 1241.488 kb on - strand at 1241.491 kb on - strand at 1242.306 kb on + strand, within rpl27 at 1242.366 kb on + strand at 1242.380 kb on - strand at 1242.513 kb on + strand, within cheY at 1242.556 kb on + strand, within cheY at 1242.563 kb on - strand, within cheY at 1242.716 kb on + strand, within cheY at 1242.795 kb on - strand at 1242.802 kb on - strand at 1242.814 kb on - strand at 1242.851 kb on - strand at 1242.862 kb on - strand at 1242.886 kb on - strand at 1242.904 kb on + strand at 1242.904 kb on + strand at 1242.912 kb on - strand at 1242.912 kb on - strand at 1242.929 kb on + strand at 1242.937 kb on + strand at 1242.973 kb on + strand at 1242.973 kb on + strand at 1242.973 kb on + strand at 1242.973 kb on + strand at 1242.973 kb on + strand at 1242.973 kb on + strand at 1242.973 kb on + strand at 1242.973 kb on + strand at 1242.981 kb on - strand at 1242.981 kb on - strand at 1242.981 kb on - strand at 1242.981 kb on - strand at 1242.981 kb on - strand at 1242.981 kb on - strand at 1242.981 kb on - strand at 1242.999 kb on - strand at 1243.008 kb on - strand at 1243.032 kb on - strand, within truB at 1243.092 kb on + strand, within truB at 1243.185 kb on + strand, within truB at 1243.288 kb on - strand, within truB at 1243.291 kb on - strand, within truB at 1243.342 kb on + strand, within truB at 1243.345 kb on - strand, within truB at 1243.355 kb on - strand, within truB at 1243.373 kb on - strand, within truB at 1243.436 kb on - strand, within truB at 1243.462 kb on + strand, within truB at 1243.470 kb on + strand, within truB at 1243.470 kb on + strand, within truB at 1243.470 kb on + strand, within truB at 1243.470 kb on + strand, within truB at 1243.478 kb on - strand, within truB at 1243.499 kb on + strand, within truB at 1243.507 kb on - strand, within truB at 1243.515 kb on - strand, within truB at 1243.532 kb on + strand, within truB at 1243.532 kb on + strand, within truB at 1243.532 kb on - strand at 1243.540 kb on - strand, within truB at 1243.556 kb on - strand, within truB at 1243.567 kb on - strand, within truB at 1243.598 kb on - strand, within truB at 1243.598 kb on - strand, within truB at 1243.638 kb on - strand, within truB at 1243.670 kb on + strand, within truB at 1243.674 kb on + strand, within truB at 1243.704 kb on - strand, within truB at 1243.706 kb on + strand, within truB at 1243.706 kb on + strand, within truB at 1243.719 kb on - strand, within truB at 1243.743 kb on + strand at 1243.751 kb on - strand at 1243.751 kb on - strand at 1243.774 kb on - strand at 1243.776 kb on + strand at 1243.782 kb on + strand at 1243.812 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ceftazidime 0.0625 mg/ml remove 1,241,480 + -1.1 1,241,480 + -1.1 1,241,488 - +1.1 1,241,491 - +0.9 1,242,306 + rpl27 Synpcc7942_1220 0.90 -3.1 1,242,366 + -1.1 1,242,380 - -0.1 1,242,513 + cheY Synpcc7942_1221 0.15 +2.0 1,242,556 + cheY Synpcc7942_1221 0.27 -0.3 1,242,563 - cheY Synpcc7942_1221 0.29 -1.1 1,242,716 + cheY Synpcc7942_1221 0.69 +0.8 1,242,795 - +1.0 1,242,802 - -0.1 1,242,814 - -0.1 1,242,851 - -0.7 1,242,862 - +1.1 1,242,886 - -0.8 1,242,904 + -0.5 1,242,904 + +1.2 1,242,912 - -2.4 1,242,912 - +1.9 1,242,929 + +0.4 1,242,937 + -0.8 1,242,973 + -0.1 1,242,973 + +2.8 1,242,973 + -1.7 1,242,973 + -0.3 1,242,973 + -1.7 1,242,973 + -1.1 1,242,973 + -0.7 1,242,973 + -1.4 1,242,981 - -1.7 1,242,981 - +0.7 1,242,981 - +0.4 1,242,981 - -1.3 1,242,981 - +2.0 1,242,981 - +0.5 1,242,981 - +0.5 1,242,999 - -1.1 1,243,008 - -1.1 1,243,032 - truB Synpcc7942_1222 0.12 +2.0 1,243,092 + truB Synpcc7942_1222 0.19 -0.5 1,243,185 + truB Synpcc7942_1222 0.29 +0.1 1,243,288 - truB Synpcc7942_1222 0.41 +2.4 1,243,291 - truB Synpcc7942_1222 0.41 +1.5 1,243,342 + truB Synpcc7942_1222 0.47 +1.5 1,243,345 - truB Synpcc7942_1222 0.47 -0.5 1,243,355 - truB Synpcc7942_1222 0.49 +0.1 1,243,373 - truB Synpcc7942_1222 0.51 +1.4 1,243,436 - truB Synpcc7942_1222 0.58 -0.1 1,243,462 + truB Synpcc7942_1222 0.61 -2.1 1,243,470 + truB Synpcc7942_1222 0.62 -0.4 1,243,470 + truB Synpcc7942_1222 0.62 -0.1 1,243,470 + truB Synpcc7942_1222 0.62 +1.0 1,243,470 + truB Synpcc7942_1222 0.62 -1.7 1,243,478 - truB Synpcc7942_1222 0.62 +1.3 1,243,499 + truB Synpcc7942_1222 0.65 +0.5 1,243,507 - truB Synpcc7942_1222 0.66 -1.2 1,243,515 - truB Synpcc7942_1222 0.67 +0.4 1,243,532 + truB Synpcc7942_1222 0.69 +2.2 1,243,532 + truB Synpcc7942_1222 0.69 -1.1 1,243,532 - +0.5 1,243,540 - truB Synpcc7942_1222 0.69 -1.3 1,243,556 - truB Synpcc7942_1222 0.71 -0.1 1,243,567 - truB Synpcc7942_1222 0.73 +0.7 1,243,598 - truB Synpcc7942_1222 0.76 +1.7 1,243,598 - truB Synpcc7942_1222 0.76 -1.1 1,243,638 - truB Synpcc7942_1222 0.81 -1.4 1,243,670 + truB Synpcc7942_1222 0.84 +0.7 1,243,674 + truB Synpcc7942_1222 0.85 +2.3 1,243,704 - truB Synpcc7942_1222 0.88 -0.1 1,243,706 + truB Synpcc7942_1222 0.88 +1.7 1,243,706 + truB Synpcc7942_1222 0.88 -0.9 1,243,719 - truB Synpcc7942_1222 0.90 -2.4 1,243,743 + +3.3 1,243,751 - +1.1 1,243,751 - -0.6 1,243,774 - +1.0 1,243,776 + +0.8 1,243,782 + +1.9 1,243,812 - -1.7
Or see this region's nucleotide sequence