Experiment: Ceftazidime 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hemD and coaE overlap by 22 nucleotides coaE and Synpcc7942_0274 are separated by 37 nucleotides Synpcc7942_0274 and Synpcc7942_0275 overlap by 11 nucleotides Synpcc7942_0275 and glcD are separated by 11 nucleotides
Synpcc7942_0272: hemD - Uroporphyrinogen-III synthase, at 267,889 to 268,683
hemD
Synpcc7942_0273: coaE - Dephospho-CoA kinase, at 268,662 to 269,291
coaE
Synpcc7942_0274: Synpcc7942_0274 - hypothetical protein, at 269,329 to 269,667
_0274
Synpcc7942_0275: Synpcc7942_0275 - hypothetical protein, at 269,657 to 269,989
_0275
Synpcc7942_0276: glcD - glycolate oxidase subunit GlcD, at 270,001 to 271,473
glcD
Position (kb) 269
270 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 268.704 kb on + strand at 269.351 kb on - strand at 269.804 kb on + strand, within Synpcc7942_0275 at 270.162 kb on + strand, within glcD at 270.219 kb on + strand, within glcD at 270.273 kb on - strand, within glcD at 270.395 kb on - strand, within glcD at 270.411 kb on + strand, within glcD at 270.419 kb on - strand, within glcD at 270.431 kb on + strand, within glcD at 270.497 kb on - strand, within glcD at 270.598 kb on + strand, within glcD at 270.598 kb on + strand, within glcD at 270.701 kb on - strand, within glcD at 270.701 kb on - strand, within glcD at 270.730 kb on + strand, within glcD at 270.738 kb on - strand, within glcD at 270.753 kb on + strand, within glcD at 270.854 kb on - strand, within glcD at 270.881 kb on - strand, within glcD at 270.881 kb on - strand, within glcD at 270.881 kb on - strand, within glcD at 270.881 kb on - strand, within glcD at 270.881 kb on - strand, within glcD at 270.891 kb on - strand, within glcD at 270.897 kb on - strand, within glcD at 270.931 kb on - strand, within glcD at 270.941 kb on - strand, within glcD at 270.953 kb on - strand, within glcD at 270.982 kb on + strand, within glcD at 270.988 kb on - strand, within glcD
Per-strain Table
Position Strand Gene LocusTag Fraction Ceftazidime 0.0625 mg/ml remove 268,704 + +0.4 269,351 - -1.6 269,804 + Synpcc7942_0275 0.44 -4.2 270,162 + glcD Synpcc7942_0276 0.11 +1.1 270,219 + glcD Synpcc7942_0276 0.15 -0.6 270,273 - glcD Synpcc7942_0276 0.18 +0.6 270,395 - glcD Synpcc7942_0276 0.27 -0.6 270,411 + glcD Synpcc7942_0276 0.28 -0.6 270,419 - glcD Synpcc7942_0276 0.28 +0.2 270,431 + glcD Synpcc7942_0276 0.29 +1.7 270,497 - glcD Synpcc7942_0276 0.34 -0.1 270,598 + glcD Synpcc7942_0276 0.41 -2.1 270,598 + glcD Synpcc7942_0276 0.41 +1.0 270,701 - glcD Synpcc7942_0276 0.48 -0.3 270,701 - glcD Synpcc7942_0276 0.48 +1.2 270,730 + glcD Synpcc7942_0276 0.49 +1.2 270,738 - glcD Synpcc7942_0276 0.50 +1.8 270,753 + glcD Synpcc7942_0276 0.51 -1.5 270,854 - glcD Synpcc7942_0276 0.58 -0.1 270,881 - glcD Synpcc7942_0276 0.60 -1.6 270,881 - glcD Synpcc7942_0276 0.60 +0.4 270,881 - glcD Synpcc7942_0276 0.60 -0.1 270,881 - glcD Synpcc7942_0276 0.60 +1.6 270,881 - glcD Synpcc7942_0276 0.60 +1.2 270,891 - glcD Synpcc7942_0276 0.60 -0.1 270,897 - glcD Synpcc7942_0276 0.61 -0.3 270,931 - glcD Synpcc7942_0276 0.63 -0.6 270,941 - glcD Synpcc7942_0276 0.64 -1.4 270,953 - glcD Synpcc7942_0276 0.65 -2.6 270,982 + glcD Synpcc7942_0276 0.67 +1.1 270,988 - glcD Synpcc7942_0276 0.67 +2.2
Or see this region's nucleotide sequence