Experiment: Ceftazidime 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0016 and eda are separated by 25 nucleotides eda and glyS are separated by 12 nucleotides
Synpcc7942_0016: Synpcc7942_0016 - hypothetical protein, at 15,020 to 15,604
_0016
Synpcc7942_0017: eda - keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase, at 15,630 to 16,193
eda
Synpcc7942_0018: glyS - glycyl-tRNA synthetase subunit beta, at 16,206 to 18,392
glyS
Position (kb) 15
16
17 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 14.658 kb on + strand at 14.666 kb on - strand at 14.676 kb on - strand at 14.703 kb on + strand at 14.703 kb on + strand at 14.711 kb on - strand at 14.729 kb on - strand at 14.769 kb on + strand at 14.769 kb on - strand at 14.774 kb on + strand at 14.779 kb on - strand at 14.801 kb on - strand at 14.801 kb on - strand at 14.801 kb on - strand at 14.822 kb on + strand at 14.822 kb on + strand at 14.822 kb on + strand at 14.822 kb on + strand at 14.822 kb on + strand at 14.822 kb on + strand at 14.830 kb on - strand at 14.830 kb on - strand at 14.830 kb on - strand at 14.832 kb on + strand at 14.840 kb on - strand at 14.840 kb on - strand at 14.886 kb on + strand at 14.894 kb on - strand at 14.901 kb on + strand at 14.926 kb on + strand at 14.934 kb on - strand at 14.941 kb on - strand at 15.023 kb on - strand at 15.167 kb on + strand, within Synpcc7942_0016 at 15.168 kb on - strand, within Synpcc7942_0016 at 15.175 kb on - strand, within Synpcc7942_0016 at 15.175 kb on - strand, within Synpcc7942_0016 at 15.232 kb on - strand, within Synpcc7942_0016 at 15.330 kb on - strand, within Synpcc7942_0016 at 15.425 kb on - strand, within Synpcc7942_0016 at 15.425 kb on - strand, within Synpcc7942_0016 at 15.552 kb on + strand at 15.562 kb on + strand at 15.698 kb on - strand, within eda at 15.779 kb on - strand, within eda at 15.859 kb on - strand, within eda at 15.917 kb on + strand, within eda at 15.983 kb on - strand, within eda at 15.987 kb on + strand, within eda at 16.067 kb on + strand, within eda at 16.067 kb on + strand, within eda at 16.075 kb on - strand, within eda at 16.177 kb on + strand at 16.195 kb on + strand at 16.203 kb on + strand at 16.203 kb on + strand at 16.221 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ceftazidime 0.0625 mg/ml remove 14,658 + +0.2 14,666 - -0.2 14,676 - -1.1 14,703 + +1.0 14,703 + +1.5 14,711 - +0.5 14,729 - +0.6 14,769 + +0.7 14,769 - +0.5 14,774 + -0.3 14,779 - +0.6 14,801 - -1.2 14,801 - +0.1 14,801 - +0.2 14,822 + -0.1 14,822 + -0.5 14,822 + -0.5 14,822 + +1.8 14,822 + +2.8 14,822 + -0.5 14,830 - +0.9 14,830 - +0.6 14,830 - -1.6 14,832 + -1.9 14,840 - +2.1 14,840 - -2.9 14,886 + -0.9 14,894 - +1.1 14,901 + -2.2 14,926 + +0.9 14,934 - -0.4 14,941 - -0.4 15,023 - +1.1 15,167 + Synpcc7942_0016 0.25 +0.1 15,168 - Synpcc7942_0016 0.25 +1.5 15,175 - Synpcc7942_0016 0.26 +0.5 15,175 - Synpcc7942_0016 0.26 +0.2 15,232 - Synpcc7942_0016 0.36 +0.4 15,330 - Synpcc7942_0016 0.53 -1.7 15,425 - Synpcc7942_0016 0.69 -1.0 15,425 - Synpcc7942_0016 0.69 -0.1 15,552 + +0.5 15,562 + -0.4 15,698 - eda Synpcc7942_0017 0.12 -0.1 15,779 - eda Synpcc7942_0017 0.26 -3.1 15,859 - eda Synpcc7942_0017 0.41 -3.8 15,917 + eda Synpcc7942_0017 0.51 -3.1 15,983 - eda Synpcc7942_0017 0.63 -3.8 15,987 + eda Synpcc7942_0017 0.63 -2.4 16,067 + eda Synpcc7942_0017 0.77 -1.7 16,067 + eda Synpcc7942_0017 0.77 -1.1 16,075 - eda Synpcc7942_0017 0.79 -1.4 16,177 + -1.7 16,195 + -0.4 16,203 + +1.1 16,203 + -1.1 16,221 - +0.3
Or see this region's nucleotide sequence