Experiment: L-Valine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4966 and Pf1N1B4_4967 are separated by 229 nucleotides Pf1N1B4_4967 and Pf1N1B4_4968 are separated by 170 nucleotides Pf1N1B4_4968 and Pf1N1B4_4969 are separated by 214 nucleotides
Pf1N1B4_4966 - Transcriptional regulator, LysR family, at 5,507,561 to 5,508,454
_4966
Pf1N1B4_4967 - Transcriptional regulator, at 5,508,684 to 5,509,088
_4967
Pf1N1B4_4968 - 4-carboxymuconolactone decarboxylase (EC 4.1.1.44), at 5,509,259 to 5,510,083
_4968
Pf1N1B4_4969 - FIG00956189: hypothetical protein, at 5,510,298 to 5,510,729
_4969
Position (kb)
5509
5510
5511 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5508.343 kb on + strand, within Pf1N1B4_4966 at 5508.428 kb on + strand at 5508.428 kb on + strand at 5508.471 kb on + strand at 5508.475 kb on + strand at 5508.475 kb on + strand at 5508.475 kb on + strand at 5508.475 kb on + strand at 5508.476 kb on - strand at 5508.476 kb on - strand at 5508.476 kb on - strand at 5508.489 kb on - strand at 5508.518 kb on + strand at 5508.518 kb on + strand at 5508.739 kb on - strand, within Pf1N1B4_4967 at 5509.031 kb on + strand, within Pf1N1B4_4967 at 5509.072 kb on - strand at 5509.076 kb on + strand at 5509.336 kb on - strand at 5509.336 kb on - strand at 5509.336 kb on - strand at 5509.527 kb on + strand, within Pf1N1B4_4968 at 5509.528 kb on - strand, within Pf1N1B4_4968 at 5509.547 kb on + strand, within Pf1N1B4_4968 at 5509.668 kb on - strand, within Pf1N1B4_4968 at 5509.668 kb on - strand, within Pf1N1B4_4968 at 5509.717 kb on - strand, within Pf1N1B4_4968 at 5509.717 kb on - strand, within Pf1N1B4_4968 at 5509.907 kb on - strand, within Pf1N1B4_4968 at 5509.908 kb on + strand at 5510.092 kb on + strand at 5510.092 kb on + strand at 5510.092 kb on + strand at 5510.095 kb on + strand at 5510.096 kb on - strand at 5510.096 kb on - strand at 5510.096 kb on - strand at 5510.108 kb on + strand at 5510.127 kb on - strand at 5510.285 kb on + strand at 5510.285 kb on + strand at 5510.286 kb on - strand at 5510.558 kb on - strand, within Pf1N1B4_4969 at 5510.562 kb on - strand, within Pf1N1B4_4969 at 5510.562 kb on - strand, within Pf1N1B4_4969 at 5510.681 kb on - strand, within Pf1N1B4_4969 at 5510.957 kb on + strand at 5511.058 kb on + strand at 5511.083 kb on - strand at 5511.083 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (N) remove 5,508,343 + Pf1N1B4_4966 0.87 -0.5 5,508,428 + -0.5 5,508,428 + -0.2 5,508,471 + -0.0 5,508,475 + -0.5 5,508,475 + -0.1 5,508,475 + -2.1 5,508,475 + +0.4 5,508,476 - +1.5 5,508,476 - +1.1 5,508,476 - +0.8 5,508,489 - +0.2 5,508,518 + +0.8 5,508,518 + -0.5 5,508,739 - Pf1N1B4_4967 0.14 -0.5 5,509,031 + Pf1N1B4_4967 0.86 -0.5 5,509,072 - +0.1 5,509,076 + -1.5 5,509,336 - +2.1 5,509,336 - +0.9 5,509,336 - -0.3 5,509,527 + Pf1N1B4_4968 0.32 -1.5 5,509,528 - Pf1N1B4_4968 0.33 -0.7 5,509,547 + Pf1N1B4_4968 0.35 -1.1 5,509,668 - Pf1N1B4_4968 0.50 -1.5 5,509,668 - Pf1N1B4_4968 0.50 +0.1 5,509,717 - Pf1N1B4_4968 0.56 -2.1 5,509,717 - Pf1N1B4_4968 0.56 +0.0 5,509,907 - Pf1N1B4_4968 0.79 -2.5 5,509,908 + -3.1 5,510,092 + +0.9 5,510,092 + +0.1 5,510,092 + +0.2 5,510,095 + +1.5 5,510,096 - -0.0 5,510,096 - -1.5 5,510,096 - +1.1 5,510,108 + -0.5 5,510,127 - -0.5 5,510,285 + +0.1 5,510,285 + -0.0 5,510,286 - +2.1 5,510,558 - Pf1N1B4_4969 0.60 +0.6 5,510,562 - Pf1N1B4_4969 0.61 +0.2 5,510,562 - Pf1N1B4_4969 0.61 +1.8 5,510,681 - Pf1N1B4_4969 0.89 +0.8 5,510,957 + +0.8 5,511,058 + +1.3 5,511,083 - +1.6 5,511,083 - +0.7
Or see this region's nucleotide sequence