Experiment: L-Valine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4538 and Pf1N1B4_4539 are separated by 228 nucleotides Pf1N1B4_4539 and Pf1N1B4_4540 are separated by 136 nucleotides Pf1N1B4_4540 and Pf1N1B4_4541 are separated by 18 nucleotides Pf1N1B4_4541 and Pf1N1B4_4542 overlap by 4 nucleotides
Pf1N1B4_4538 - Uncharacterized protein ImpB, at 5,005,788 to 5,006,336
_4538
Pf1N1B4_4539 - Uncharacterized protein homologous to Paeru. PA2364, at 5,006,565 to 5,007,014
_4539
Pf1N1B4_4540 - Uncharacterized protein ImpJ/VasE, at 5,007,151 to 5,008,482
_4540
Pf1N1B4_4541 - Inner membrane protein DotU, at 5,008,501 to 5,009,268
_4541
Pf1N1B4_4542 - IcmF-related protein, at 5,009,265 to 5,012,888
_4542
Position (kb) 5007
5008
5009 Strain fitness (log2 ratio) -1
0
1
2 at 5006.275 kb on + strand, within Pf1N1B4_4538 at 5006.403 kb on - strand at 5006.511 kb on - strand at 5006.910 kb on + strand, within Pf1N1B4_4539 at 5006.910 kb on + strand at 5006.910 kb on + strand, within Pf1N1B4_4539 at 5006.910 kb on + strand, within Pf1N1B4_4539 at 5006.910 kb on + strand at 5006.911 kb on - strand, within Pf1N1B4_4539 at 5006.911 kb on - strand, within Pf1N1B4_4539 at 5007.150 kb on + strand at 5007.150 kb on + strand at 5007.151 kb on - strand at 5007.161 kb on + strand at 5007.162 kb on - strand at 5007.268 kb on - strand at 5007.931 kb on + strand at 5007.932 kb on - strand, within Pf1N1B4_4540 at 5007.932 kb on - strand, within Pf1N1B4_4540 at 5008.500 kb on + strand at 5008.501 kb on - strand at 5008.501 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (N) remove 5,006,275 + Pf1N1B4_4538 0.89 -0.8 5,006,403 - -0.1 5,006,511 - -1.0 5,006,910 + Pf1N1B4_4539 0.77 -0.9 5,006,910 + +1.6 5,006,910 + Pf1N1B4_4539 0.77 +1.0 5,006,910 + Pf1N1B4_4539 0.77 +0.6 5,006,910 + -0.4 5,006,911 - Pf1N1B4_4539 0.77 +0.1 5,006,911 - Pf1N1B4_4539 0.77 +1.7 5,007,150 + -0.4 5,007,150 + +0.4 5,007,151 - +0.9 5,007,161 + -0.4 5,007,162 - +1.9 5,007,268 - +0.6 5,007,931 + -0.9 5,007,932 - Pf1N1B4_4540 0.59 -0.4 5,007,932 - Pf1N1B4_4540 0.59 -0.8 5,008,500 + -0.4 5,008,501 - -0.4 5,008,501 - +0.4
Or see this region's nucleotide sequence