Experiment: L-Valine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3888 and Pf1N1B4_3889 are separated by 60 nucleotides Pf1N1B4_3889 and Pf1N1B4_3890 overlap by 21 nucleotides
Pf1N1B4_3888 - Aconitate hydratase (EC 4.2.1.3), at 4,317,018 to 4,319,759
_3888
Pf1N1B4_3889 - hypothetical protein, at 4,319,820 to 4,320,002
_3889
Pf1N1B4_3890 - LSU rRNA 2'-O-methyl-C2498 methyltransferase RlmM, at 4,319,982 to 4,321,055
_3890
Position (kb)
4319
4320
4321 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4319.155 kb on + strand, within Pf1N1B4_3888 at 4319.155 kb on + strand, within Pf1N1B4_3888 at 4319.155 kb on + strand, within Pf1N1B4_3888 at 4319.155 kb on + strand, within Pf1N1B4_3888 at 4319.155 kb on + strand, within Pf1N1B4_3888 at 4319.155 kb on + strand, within Pf1N1B4_3888 at 4319.156 kb on - strand, within Pf1N1B4_3888 at 4319.156 kb on - strand, within Pf1N1B4_3888 at 4319.156 kb on - strand, within Pf1N1B4_3888 at 4319.156 kb on - strand, within Pf1N1B4_3888 at 4319.156 kb on - strand, within Pf1N1B4_3888 at 4319.156 kb on - strand, within Pf1N1B4_3888 at 4319.156 kb on - strand, within Pf1N1B4_3888 at 4319.201 kb on - strand, within Pf1N1B4_3888 at 4319.201 kb on - strand, within Pf1N1B4_3888 at 4319.259 kb on - strand, within Pf1N1B4_3888 at 4319.353 kb on + strand at 4319.353 kb on + strand, within Pf1N1B4_3888 at 4319.354 kb on - strand, within Pf1N1B4_3888 at 4319.354 kb on - strand, within Pf1N1B4_3888 at 4319.502 kb on + strand at 4319.701 kb on + strand at 4319.701 kb on + strand at 4319.701 kb on + strand at 4319.702 kb on - strand at 4319.721 kb on + strand at 4319.722 kb on - strand at 4319.774 kb on + strand at 4319.774 kb on + strand at 4319.774 kb on + strand at 4319.775 kb on - strand at 4319.816 kb on - strand at 4319.816 kb on - strand at 4319.831 kb on + strand at 4319.833 kb on + strand at 4319.833 kb on + strand at 4319.833 kb on + strand at 4319.834 kb on - strand at 4319.847 kb on + strand, within Pf1N1B4_3889 at 4319.859 kb on - strand, within Pf1N1B4_3889 at 4319.977 kb on + strand, within Pf1N1B4_3889 at 4319.977 kb on + strand, within Pf1N1B4_3889 at 4319.996 kb on + strand at 4320.900 kb on + strand, within Pf1N1B4_3890 at 4320.900 kb on + strand, within Pf1N1B4_3890 at 4320.900 kb on + strand, within Pf1N1B4_3890 at 4320.929 kb on + strand, within Pf1N1B4_3890 at 4320.987 kb on + strand at 4320.987 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (N) remove 4,319,155 + Pf1N1B4_3888 0.78 -0.2 4,319,155 + Pf1N1B4_3888 0.78 -2.6 4,319,155 + Pf1N1B4_3888 0.78 -1.0 4,319,155 + Pf1N1B4_3888 0.78 +1.0 4,319,155 + Pf1N1B4_3888 0.78 -2.8 4,319,155 + Pf1N1B4_3888 0.78 +1.1 4,319,156 - Pf1N1B4_3888 0.78 -0.5 4,319,156 - Pf1N1B4_3888 0.78 -2.2 4,319,156 - Pf1N1B4_3888 0.78 +1.9 4,319,156 - Pf1N1B4_3888 0.78 -0.2 4,319,156 - Pf1N1B4_3888 0.78 +0.2 4,319,156 - Pf1N1B4_3888 0.78 +1.3 4,319,156 - Pf1N1B4_3888 0.78 -0.2 4,319,201 - Pf1N1B4_3888 0.80 -1.8 4,319,201 - Pf1N1B4_3888 0.80 -1.0 4,319,259 - Pf1N1B4_3888 0.82 -1.6 4,319,353 + -2.6 4,319,353 + Pf1N1B4_3888 0.85 -0.2 4,319,354 - Pf1N1B4_3888 0.85 +0.5 4,319,354 - Pf1N1B4_3888 0.85 -0.2 4,319,502 + -0.8 4,319,701 + +0.8 4,319,701 + -0.2 4,319,701 + -1.0 4,319,702 - -0.8 4,319,721 + -1.2 4,319,722 - +1.8 4,319,774 + -1.2 4,319,774 + -0.2 4,319,774 + -0.8 4,319,775 - +1.3 4,319,816 - -0.7 4,319,816 - -0.2 4,319,831 + -0.7 4,319,833 + +0.1 4,319,833 + +1.8 4,319,833 + +0.2 4,319,834 - +0.1 4,319,847 + Pf1N1B4_3889 0.15 +0.2 4,319,859 - Pf1N1B4_3889 0.21 -1.0 4,319,977 + Pf1N1B4_3889 0.86 -1.2 4,319,977 + Pf1N1B4_3889 0.86 -0.8 4,319,996 + +0.8 4,320,900 + Pf1N1B4_3890 0.85 -0.6 4,320,900 + Pf1N1B4_3890 0.85 -0.2 4,320,900 + Pf1N1B4_3890 0.85 +2.3 4,320,929 + Pf1N1B4_3890 0.88 +1.3 4,320,987 + +1.3 4,320,987 + -0.2
Or see this region's nucleotide sequence