Experiment: L-Valine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3820 and Pf1N1B4_3821 are separated by 205 nucleotides Pf1N1B4_3821 and Pf1N1B4_3822 are separated by 51 nucleotides
Pf1N1B4_3820 - 2-methylcitrate synthase (EC 2.3.3.5), at 4,231,337 to 4,232,464
_3820
Pf1N1B4_3821 - 2-methylcitrate dehydratase FeS dependent (EC 4.2.1.79), at 4,232,670 to 4,235,264
_3821
Pf1N1B4_3822 - 2-methylaconitate cis-trans isomerase, at 4,235,316 to 4,236,506
_3822
Position (kb)
4232
4233
4234
4235
4236 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4231.743 kb on - strand, within Pf1N1B4_3820 at 4231.955 kb on + strand, within Pf1N1B4_3820 at 4231.956 kb on - strand, within Pf1N1B4_3820 at 4231.956 kb on - strand, within Pf1N1B4_3820 at 4231.956 kb on - strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.127 kb on - strand, within Pf1N1B4_3820 at 4232.127 kb on - strand, within Pf1N1B4_3820 at 4232.127 kb on - strand, within Pf1N1B4_3820 at 4232.127 kb on - strand, within Pf1N1B4_3820 at 4232.127 kb on - strand at 4232.127 kb on - strand, within Pf1N1B4_3820 at 4232.128 kb on + strand, within Pf1N1B4_3820 at 4232.129 kb on - strand, within Pf1N1B4_3820 at 4232.276 kb on + strand, within Pf1N1B4_3820 at 4232.276 kb on + strand, within Pf1N1B4_3820 at 4232.276 kb on + strand, within Pf1N1B4_3820 at 4232.276 kb on + strand, within Pf1N1B4_3820 at 4232.276 kb on + strand, within Pf1N1B4_3820 at 4232.276 kb on + strand, within Pf1N1B4_3820 at 4232.276 kb on + strand, within Pf1N1B4_3820 at 4232.276 kb on + strand, within Pf1N1B4_3820 at 4232.277 kb on - strand, within Pf1N1B4_3820 at 4232.277 kb on - strand, within Pf1N1B4_3820 at 4232.277 kb on - strand, within Pf1N1B4_3820 at 4232.292 kb on - strand, within Pf1N1B4_3820 at 4232.417 kb on + strand at 4232.417 kb on + strand at 4232.417 kb on + strand at 4232.418 kb on - strand at 4232.418 kb on - strand at 4232.418 kb on - strand at 4232.619 kb on + strand at 4232.620 kb on - strand at 4232.620 kb on - strand at 4232.646 kb on - strand at 4232.902 kb on + strand at 4232.903 kb on - strand at 4233.660 kb on + strand at 4233.709 kb on - strand, within Pf1N1B4_3821 at 4234.155 kb on + strand, within Pf1N1B4_3821 at 4234.297 kb on + strand at 4234.297 kb on + strand, within Pf1N1B4_3821 at 4234.298 kb on - strand, within Pf1N1B4_3821 at 4234.410 kb on + strand at 4234.410 kb on + strand, within Pf1N1B4_3821 at 4234.410 kb on + strand, within Pf1N1B4_3821 at 4234.411 kb on - strand, within Pf1N1B4_3821 at 4234.464 kb on + strand, within Pf1N1B4_3821 at 4234.488 kb on + strand, within Pf1N1B4_3821 at 4234.488 kb on + strand, within Pf1N1B4_3821 at 4234.488 kb on + strand, within Pf1N1B4_3821 at 4234.488 kb on + strand, within Pf1N1B4_3821 at 4234.488 kb on + strand, within Pf1N1B4_3821 at 4234.488 kb on + strand, within Pf1N1B4_3821 at 4234.488 kb on + strand at 4234.489 kb on - strand, within Pf1N1B4_3821 at 4234.489 kb on - strand, within Pf1N1B4_3821 at 4234.489 kb on - strand, within Pf1N1B4_3821 at 4234.680 kb on + strand, within Pf1N1B4_3821 at 4234.680 kb on + strand, within Pf1N1B4_3821 at 4234.681 kb on - strand, within Pf1N1B4_3821 at 4234.845 kb on + strand, within Pf1N1B4_3821 at 4234.846 kb on - strand, within Pf1N1B4_3821 at 4234.846 kb on - strand, within Pf1N1B4_3821 at 4235.073 kb on + strand at 4235.196 kb on + strand at 4235.313 kb on + strand at 4235.313 kb on + strand at 4235.313 kb on + strand at 4235.313 kb on + strand at 4235.313 kb on + strand at 4235.313 kb on + strand at 4235.313 kb on + strand at 4235.313 kb on + strand at 4235.313 kb on + strand at 4235.314 kb on - strand at 4235.314 kb on - strand at 4235.352 kb on + strand at 4235.352 kb on + strand at 4235.352 kb on + strand at 4235.352 kb on + strand at 4235.352 kb on + strand at 4235.353 kb on - strand at 4235.353 kb on - strand at 4235.761 kb on + strand, within Pf1N1B4_3822 at 4236.177 kb on + strand, within Pf1N1B4_3822
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (N) remove 4,231,743 - Pf1N1B4_3820 0.36 -0.1 4,231,955 + Pf1N1B4_3820 0.55 +1.9 4,231,956 - Pf1N1B4_3820 0.55 +0.6 4,231,956 - Pf1N1B4_3820 0.55 -0.1 4,231,956 - Pf1N1B4_3820 0.55 -0.9 4,232,126 + Pf1N1B4_3820 0.70 -0.8 4,232,126 + Pf1N1B4_3820 0.70 -0.3 4,232,126 + Pf1N1B4_3820 0.70 +1.5 4,232,126 + Pf1N1B4_3820 0.70 -2.5 4,232,126 + Pf1N1B4_3820 0.70 +0.9 4,232,126 + Pf1N1B4_3820 0.70 +0.4 4,232,126 + -2.1 4,232,126 + Pf1N1B4_3820 0.70 -0.1 4,232,126 + Pf1N1B4_3820 0.70 -0.1 4,232,127 - Pf1N1B4_3820 0.70 -1.1 4,232,127 - Pf1N1B4_3820 0.70 -2.1 4,232,127 - Pf1N1B4_3820 0.70 -1.1 4,232,127 - Pf1N1B4_3820 0.70 -0.5 4,232,127 - -0.1 4,232,127 - Pf1N1B4_3820 0.70 +0.3 4,232,128 + Pf1N1B4_3820 0.70 -0.5 4,232,129 - Pf1N1B4_3820 0.70 +0.5 4,232,276 + Pf1N1B4_3820 0.83 +0.9 4,232,276 + Pf1N1B4_3820 0.83 -0.6 4,232,276 + Pf1N1B4_3820 0.83 -1.1 4,232,276 + Pf1N1B4_3820 0.83 -0.9 4,232,276 + Pf1N1B4_3820 0.83 -0.1 4,232,276 + Pf1N1B4_3820 0.83 -0.7 4,232,276 + Pf1N1B4_3820 0.83 -0.5 4,232,276 + Pf1N1B4_3820 0.83 -0.5 4,232,277 - Pf1N1B4_3820 0.83 -1.4 4,232,277 - Pf1N1B4_3820 0.83 +0.4 4,232,277 - Pf1N1B4_3820 0.83 +0.9 4,232,292 - Pf1N1B4_3820 0.85 -0.3 4,232,417 + -0.7 4,232,417 + -0.1 4,232,417 + -3.1 4,232,418 - -0.1 4,232,418 - +0.2 4,232,418 - +0.9 4,232,619 + -0.2 4,232,620 - -3.1 4,232,620 - -1.1 4,232,646 - -1.7 4,232,902 + -0.1 4,232,903 - -1.4 4,233,660 + +0.9 4,233,709 - Pf1N1B4_3821 0.40 +1.7 4,234,155 + Pf1N1B4_3821 0.57 -2.5 4,234,297 + -1.1 4,234,297 + Pf1N1B4_3821 0.63 -1.1 4,234,298 - Pf1N1B4_3821 0.63 -2.7 4,234,410 + -0.1 4,234,410 + Pf1N1B4_3821 0.67 -1.7 4,234,410 + Pf1N1B4_3821 0.67 -2.7 4,234,411 - Pf1N1B4_3821 0.67 -1.1 4,234,464 + Pf1N1B4_3821 0.69 -1.1 4,234,488 + Pf1N1B4_3821 0.70 -1.1 4,234,488 + Pf1N1B4_3821 0.70 -2.3 4,234,488 + Pf1N1B4_3821 0.70 -2.9 4,234,488 + Pf1N1B4_3821 0.70 -2.9 4,234,488 + Pf1N1B4_3821 0.70 -2.1 4,234,488 + Pf1N1B4_3821 0.70 -2.7 4,234,488 + -1.1 4,234,489 - Pf1N1B4_3821 0.70 -0.1 4,234,489 - Pf1N1B4_3821 0.70 -1.1 4,234,489 - Pf1N1B4_3821 0.70 -1.4 4,234,680 + Pf1N1B4_3821 0.77 -2.5 4,234,680 + Pf1N1B4_3821 0.77 -0.1 4,234,681 - Pf1N1B4_3821 0.77 -1.7 4,234,845 + Pf1N1B4_3821 0.84 -1.7 4,234,846 - Pf1N1B4_3821 0.84 -2.1 4,234,846 - Pf1N1B4_3821 0.84 -2.7 4,235,073 + +0.9 4,235,196 + -0.1 4,235,313 + -0.1 4,235,313 + +0.9 4,235,313 + -1.1 4,235,313 + -1.7 4,235,313 + -2.3 4,235,313 + -3.0 4,235,313 + -0.1 4,235,313 + -1.7 4,235,313 + -1.1 4,235,314 - -0.1 4,235,314 - -2.3 4,235,352 + -2.1 4,235,352 + -1.1 4,235,352 + -1.7 4,235,352 + -1.5 4,235,352 + +0.5 4,235,353 - +1.2 4,235,353 - -0.1 4,235,761 + Pf1N1B4_3822 0.37 -3.3 4,236,177 + Pf1N1B4_3822 0.72 -2.1
Or see this region's nucleotide sequence