Experiment: L-Valine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2238 and Pf1N1B4_2239 are separated by 90 nucleotides Pf1N1B4_2239 and Pf1N1B4_2240 are separated by 126 nucleotides Pf1N1B4_2240 and Pf1N1B4_2241 are separated by 124 nucleotides
Pf1N1B4_2238 - Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB, at 2,552,373 to 2,554,157
_2238
Pf1N1B4_2239 - FIG00953395: hypothetical protein, at 2,554,248 to 2,554,586
_2239
Pf1N1B4_2240 - Transcriptional regulator, AsnC family, at 2,554,713 to 2,555,177
_2240
Pf1N1B4_2241 - Adenylate cyclase (EC 4.6.1.1), at 2,555,302 to 2,556,669
_2241
Position (kb)
2554
2555
2556 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2553.844 kb on + strand, within Pf1N1B4_2238 at 2553.844 kb on + strand, within Pf1N1B4_2238 at 2553.844 kb on + strand, within Pf1N1B4_2238 at 2553.845 kb on - strand, within Pf1N1B4_2238 at 2553.901 kb on + strand at 2553.901 kb on + strand, within Pf1N1B4_2238 at 2553.901 kb on + strand, within Pf1N1B4_2238 at 2553.901 kb on + strand, within Pf1N1B4_2238 at 2553.901 kb on + strand, within Pf1N1B4_2238 at 2553.901 kb on + strand, within Pf1N1B4_2238 at 2553.902 kb on - strand, within Pf1N1B4_2238 at 2553.902 kb on - strand, within Pf1N1B4_2238 at 2554.026 kb on + strand at 2554.026 kb on + strand at 2554.026 kb on + strand at 2554.026 kb on + strand at 2554.027 kb on - strand at 2554.027 kb on - strand at 2554.168 kb on + strand at 2554.168 kb on + strand at 2554.169 kb on - strand at 2554.326 kb on - strand, within Pf1N1B4_2239 at 2554.326 kb on - strand, within Pf1N1B4_2239 at 2554.329 kb on + strand, within Pf1N1B4_2239 at 2554.330 kb on - strand, within Pf1N1B4_2239 at 2554.330 kb on - strand, within Pf1N1B4_2239 at 2554.497 kb on + strand, within Pf1N1B4_2239 at 2554.497 kb on + strand, within Pf1N1B4_2239 at 2554.498 kb on - strand, within Pf1N1B4_2239 at 2554.498 kb on - strand, within Pf1N1B4_2239 at 2554.498 kb on - strand, within Pf1N1B4_2239 at 2554.498 kb on - strand, within Pf1N1B4_2239 at 2554.498 kb on - strand, within Pf1N1B4_2239 at 2554.498 kb on - strand, within Pf1N1B4_2239 at 2554.504 kb on + strand, within Pf1N1B4_2239 at 2554.504 kb on + strand, within Pf1N1B4_2239 at 2554.575 kb on + strand at 2554.575 kb on + strand at 2554.575 kb on + strand at 2554.575 kb on + strand at 2554.576 kb on - strand at 2554.641 kb on + strand at 2554.645 kb on + strand at 2554.645 kb on + strand at 2554.645 kb on + strand at 2554.646 kb on - strand at 2554.646 kb on - strand at 2554.691 kb on - strand at 2554.691 kb on - strand at 2554.702 kb on + strand at 2554.702 kb on + strand at 2554.712 kb on + strand at 2555.010 kb on + strand, within Pf1N1B4_2240 at 2555.011 kb on - strand, within Pf1N1B4_2240 at 2555.011 kb on - strand, within Pf1N1B4_2240 at 2555.011 kb on - strand, within Pf1N1B4_2240 at 2555.070 kb on + strand, within Pf1N1B4_2240 at 2555.070 kb on + strand, within Pf1N1B4_2240 at 2555.070 kb on + strand, within Pf1N1B4_2240 at 2555.070 kb on + strand, within Pf1N1B4_2240 at 2555.071 kb on - strand, within Pf1N1B4_2240 at 2555.071 kb on - strand, within Pf1N1B4_2240 at 2555.250 kb on + strand at 2555.250 kb on + strand at 2555.250 kb on + strand at 2555.303 kb on + strand at 2555.303 kb on + strand at 2555.304 kb on - strand at 2555.339 kb on + strand at 2555.339 kb on + strand at 2555.340 kb on - strand at 2555.429 kb on + strand at 2555.430 kb on - strand at 2555.430 kb on - strand at 2555.430 kb on - strand at 2555.430 kb on - strand at 2555.742 kb on + strand, within Pf1N1B4_2241 at 2555.742 kb on + strand, within Pf1N1B4_2241 at 2555.742 kb on + strand, within Pf1N1B4_2241 at 2555.743 kb on - strand, within Pf1N1B4_2241 at 2555.743 kb on - strand at 2555.746 kb on + strand, within Pf1N1B4_2241 at 2555.746 kb on + strand at 2555.746 kb on + strand, within Pf1N1B4_2241 at 2555.746 kb on + strand, within Pf1N1B4_2241 at 2555.746 kb on + strand, within Pf1N1B4_2241 at 2555.746 kb on + strand, within Pf1N1B4_2241 at 2555.747 kb on - strand, within Pf1N1B4_2241 at 2555.747 kb on - strand, within Pf1N1B4_2241 at 2555.747 kb on - strand, within Pf1N1B4_2241 at 2555.747 kb on - strand, within Pf1N1B4_2241 at 2555.747 kb on - strand, within Pf1N1B4_2241 at 2555.747 kb on - strand, within Pf1N1B4_2241 at 2555.773 kb on + strand, within Pf1N1B4_2241 at 2555.774 kb on - strand, within Pf1N1B4_2241 at 2555.774 kb on - strand, within Pf1N1B4_2241 at 2555.933 kb on + strand, within Pf1N1B4_2241 at 2556.044 kb on + strand, within Pf1N1B4_2241 at 2556.045 kb on - strand, within Pf1N1B4_2241 at 2556.045 kb on - strand, within Pf1N1B4_2241 at 2556.139 kb on + strand, within Pf1N1B4_2241 at 2556.140 kb on - strand, within Pf1N1B4_2241 at 2556.140 kb on - strand, within Pf1N1B4_2241
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (N) remove 2,553,844 + Pf1N1B4_2238 0.82 +1.6 2,553,844 + Pf1N1B4_2238 0.82 -0.8 2,553,844 + Pf1N1B4_2238 0.82 +1.5 2,553,845 - Pf1N1B4_2238 0.82 -0.0 2,553,901 + -0.8 2,553,901 + Pf1N1B4_2238 0.86 +1.6 2,553,901 + Pf1N1B4_2238 0.86 -0.8 2,553,901 + Pf1N1B4_2238 0.86 +0.2 2,553,901 + Pf1N1B4_2238 0.86 +0.7 2,553,901 + Pf1N1B4_2238 0.86 +0.2 2,553,902 - Pf1N1B4_2238 0.86 -0.8 2,553,902 - Pf1N1B4_2238 0.86 +0.7 2,554,026 + +0.9 2,554,026 + +1.4 2,554,026 + -0.8 2,554,026 + -0.5 2,554,027 - -0.0 2,554,027 - -0.1 2,554,168 + -0.8 2,554,168 + +0.5 2,554,169 - -0.7 2,554,326 - Pf1N1B4_2239 0.23 -1.7 2,554,326 - Pf1N1B4_2239 0.23 +0.0 2,554,329 + Pf1N1B4_2239 0.24 -0.8 2,554,330 - Pf1N1B4_2239 0.24 -3.1 2,554,330 - Pf1N1B4_2239 0.24 -0.8 2,554,497 + Pf1N1B4_2239 0.73 +2.2 2,554,497 + Pf1N1B4_2239 0.73 -2.3 2,554,498 - Pf1N1B4_2239 0.74 +0.2 2,554,498 - Pf1N1B4_2239 0.74 -1.3 2,554,498 - Pf1N1B4_2239 0.74 +0.2 2,554,498 - Pf1N1B4_2239 0.74 -0.8 2,554,498 - Pf1N1B4_2239 0.74 -0.2 2,554,498 - Pf1N1B4_2239 0.74 -2.9 2,554,504 + Pf1N1B4_2239 0.76 -1.3 2,554,504 + Pf1N1B4_2239 0.76 -2.0 2,554,575 + -1.0 2,554,575 + -2.1 2,554,575 + -0.4 2,554,575 + -1.3 2,554,576 - +0.2 2,554,641 + -0.8 2,554,645 + +0.1 2,554,645 + -1.1 2,554,645 + +0.2 2,554,646 - +0.2 2,554,646 - +0.7 2,554,691 - -0.5 2,554,691 - -0.2 2,554,702 + -0.5 2,554,702 + -1.3 2,554,712 + +0.8 2,555,010 + Pf1N1B4_2240 0.64 +1.7 2,555,011 - Pf1N1B4_2240 0.64 -0.9 2,555,011 - Pf1N1B4_2240 0.64 -0.2 2,555,011 - Pf1N1B4_2240 0.64 -1.3 2,555,070 + Pf1N1B4_2240 0.77 -2.1 2,555,070 + Pf1N1B4_2240 0.77 -1.6 2,555,070 + Pf1N1B4_2240 0.77 -1.8 2,555,070 + Pf1N1B4_2240 0.77 -0.0 2,555,071 - Pf1N1B4_2240 0.77 -1.2 2,555,071 - Pf1N1B4_2240 0.77 +0.8 2,555,250 + +0.2 2,555,250 + +0.2 2,555,250 + -0.5 2,555,303 + -1.5 2,555,303 + +1.5 2,555,304 - +1.8 2,555,339 + +0.2 2,555,339 + +0.2 2,555,340 - +0.2 2,555,429 + -0.5 2,555,430 - -0.3 2,555,430 - -0.8 2,555,430 - -1.8 2,555,430 - +0.5 2,555,742 + Pf1N1B4_2241 0.32 -0.3 2,555,742 + Pf1N1B4_2241 0.32 +0.2 2,555,742 + Pf1N1B4_2241 0.32 -2.3 2,555,743 - Pf1N1B4_2241 0.32 +0.5 2,555,743 - -0.8 2,555,746 + Pf1N1B4_2241 0.32 -1.0 2,555,746 + -0.3 2,555,746 + Pf1N1B4_2241 0.32 -2.1 2,555,746 + Pf1N1B4_2241 0.32 -1.3 2,555,746 + Pf1N1B4_2241 0.32 +0.2 2,555,746 + Pf1N1B4_2241 0.32 -1.1 2,555,747 - Pf1N1B4_2241 0.33 -1.8 2,555,747 - Pf1N1B4_2241 0.33 -2.5 2,555,747 - Pf1N1B4_2241 0.33 -1.1 2,555,747 - Pf1N1B4_2241 0.33 +0.9 2,555,747 - Pf1N1B4_2241 0.33 +0.2 2,555,747 - Pf1N1B4_2241 0.33 +0.5 2,555,773 + Pf1N1B4_2241 0.34 +1.2 2,555,774 - Pf1N1B4_2241 0.35 -0.0 2,555,774 - Pf1N1B4_2241 0.35 +0.2 2,555,933 + Pf1N1B4_2241 0.46 +0.1 2,556,044 + Pf1N1B4_2241 0.54 +1.8 2,556,045 - Pf1N1B4_2241 0.54 -0.3 2,556,045 - Pf1N1B4_2241 0.54 +0.6 2,556,139 + Pf1N1B4_2241 0.61 +0.2 2,556,140 - Pf1N1B4_2241 0.61 -0.3 2,556,140 - Pf1N1B4_2241 0.61 -2.1
Or see this region's nucleotide sequence