Experiment: L-Valine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1389 and Pf1N1B4_1390 are separated by 68 nucleotides Pf1N1B4_1390 and Pf1N1B4_1391 are separated by 220 nucleotides Pf1N1B4_1391 and Pf1N1B4_1392 overlap by 1 nucleotides
Pf1N1B4_1389 - Lysine decarboxylase family, at 1,589,605 to 1,590,192
_1389
Pf1N1B4_1390 - FIG00956548: hypothetical protein, at 1,590,261 to 1,590,449
_1390
Pf1N1B4_1391 - Protein containing transglutaminase-like domain, putative cysteine protease, at 1,590,670 to 1,591,560
_1391
Pf1N1B4_1392 - Protein containing domains DUF404, DUF407, DUF403, at 1,591,560 to 1,594,046
_1392
Position (kb)
1590
1591
1592 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1589.913 kb on + strand, within Pf1N1B4_1389 at 1589.913 kb on + strand, within Pf1N1B4_1389 at 1589.914 kb on - strand, within Pf1N1B4_1389 at 1590.014 kb on + strand, within Pf1N1B4_1389 at 1590.014 kb on + strand, within Pf1N1B4_1389 at 1590.015 kb on - strand, within Pf1N1B4_1389 at 1590.055 kb on - strand, within Pf1N1B4_1389 at 1590.055 kb on - strand, within Pf1N1B4_1389 at 1590.267 kb on - strand at 1590.272 kb on + strand at 1590.272 kb on + strand at 1590.272 kb on + strand at 1590.272 kb on + strand at 1590.272 kb on + strand at 1590.273 kb on - strand at 1590.273 kb on - strand at 1590.276 kb on + strand at 1590.276 kb on + strand at 1590.276 kb on + strand at 1590.276 kb on + strand at 1590.276 kb on + strand at 1590.277 kb on - strand at 1590.277 kb on - strand at 1590.277 kb on - strand at 1590.277 kb on - strand at 1590.350 kb on + strand, within Pf1N1B4_1390 at 1590.350 kb on + strand, within Pf1N1B4_1390 at 1590.350 kb on + strand, within Pf1N1B4_1390 at 1590.350 kb on + strand, within Pf1N1B4_1390 at 1590.350 kb on + strand, within Pf1N1B4_1390 at 1590.350 kb on + strand, within Pf1N1B4_1390 at 1590.350 kb on + strand, within Pf1N1B4_1390 at 1590.351 kb on - strand, within Pf1N1B4_1390 at 1590.685 kb on + strand at 1590.686 kb on - strand at 1590.686 kb on - strand at 1590.686 kb on - strand at 1590.935 kb on + strand, within Pf1N1B4_1391 at 1591.175 kb on + strand, within Pf1N1B4_1391 at 1591.175 kb on + strand, within Pf1N1B4_1391 at 1591.175 kb on + strand, within Pf1N1B4_1391 at 1591.175 kb on + strand, within Pf1N1B4_1391 at 1591.175 kb on + strand, within Pf1N1B4_1391 at 1591.175 kb on + strand, within Pf1N1B4_1391 at 1591.175 kb on + strand, within Pf1N1B4_1391 at 1591.175 kb on + strand, within Pf1N1B4_1391 at 1591.176 kb on - strand, within Pf1N1B4_1391 at 1591.176 kb on - strand, within Pf1N1B4_1391 at 1591.176 kb on - strand, within Pf1N1B4_1391 at 1591.176 kb on - strand, within Pf1N1B4_1391 at 1591.176 kb on - strand, within Pf1N1B4_1391 at 1591.238 kb on + strand, within Pf1N1B4_1391 at 1591.239 kb on - strand, within Pf1N1B4_1391 at 1591.239 kb on - strand, within Pf1N1B4_1391 at 1591.380 kb on + strand, within Pf1N1B4_1391 at 1591.517 kb on + strand at 1591.518 kb on - strand at 1591.518 kb on - strand at 1591.518 kb on - strand at 1591.518 kb on - strand at 1591.519 kb on - strand at 1591.524 kb on - strand at 1591.524 kb on - strand at 1591.680 kb on + strand at 1591.681 kb on - strand at 1592.247 kb on + strand, within Pf1N1B4_1392 at 1592.426 kb on - strand, within Pf1N1B4_1392 at 1592.426 kb on - strand, within Pf1N1B4_1392 at 1592.524 kb on + strand, within Pf1N1B4_1392 at 1592.524 kb on + strand, within Pf1N1B4_1392 at 1592.559 kb on - strand, within Pf1N1B4_1392 at 1592.559 kb on - strand, within Pf1N1B4_1392
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (N) remove 1,589,913 + Pf1N1B4_1389 0.52 +0.1 1,589,913 + Pf1N1B4_1389 0.52 -0.3 1,589,914 - Pf1N1B4_1389 0.53 +2.9 1,590,014 + Pf1N1B4_1389 0.70 -2.2 1,590,014 + Pf1N1B4_1389 0.70 +0.6 1,590,015 - Pf1N1B4_1389 0.70 +0.6 1,590,055 - Pf1N1B4_1389 0.77 +0.1 1,590,055 - Pf1N1B4_1389 0.77 -0.7 1,590,267 - +1.3 1,590,272 + -0.1 1,590,272 + +0.1 1,590,272 + -1.9 1,590,272 + +0.1 1,590,272 + +1.3 1,590,273 - +1.1 1,590,273 - -1.7 1,590,276 + +0.1 1,590,276 + -0.4 1,590,276 + -0.1 1,590,276 + +0.8 1,590,276 + +1.1 1,590,277 - -0.9 1,590,277 - +0.1 1,590,277 - -0.4 1,590,277 - -1.0 1,590,350 + Pf1N1B4_1390 0.47 +3.3 1,590,350 + Pf1N1B4_1390 0.47 -0.3 1,590,350 + Pf1N1B4_1390 0.47 +0.1 1,590,350 + Pf1N1B4_1390 0.47 +0.1 1,590,350 + Pf1N1B4_1390 0.47 +0.4 1,590,350 + Pf1N1B4_1390 0.47 +0.7 1,590,350 + Pf1N1B4_1390 0.47 +0.7 1,590,351 - Pf1N1B4_1390 0.48 +0.1 1,590,685 + +0.1 1,590,686 - -0.6 1,590,686 - +0.1 1,590,686 - +0.2 1,590,935 + Pf1N1B4_1391 0.30 -1.3 1,591,175 + Pf1N1B4_1391 0.57 +0.4 1,591,175 + Pf1N1B4_1391 0.57 -1.1 1,591,175 + Pf1N1B4_1391 0.57 +0.1 1,591,175 + Pf1N1B4_1391 0.57 -1.9 1,591,175 + Pf1N1B4_1391 0.57 -1.5 1,591,175 + Pf1N1B4_1391 0.57 -2.2 1,591,175 + Pf1N1B4_1391 0.57 -0.4 1,591,175 + Pf1N1B4_1391 0.57 -1.4 1,591,176 - Pf1N1B4_1391 0.57 -0.1 1,591,176 - Pf1N1B4_1391 0.57 -0.9 1,591,176 - Pf1N1B4_1391 0.57 -1.4 1,591,176 - Pf1N1B4_1391 0.57 -1.2 1,591,176 - Pf1N1B4_1391 0.57 -0.9 1,591,238 + Pf1N1B4_1391 0.64 +0.3 1,591,239 - Pf1N1B4_1391 0.64 +0.1 1,591,239 - Pf1N1B4_1391 0.64 -0.9 1,591,380 + Pf1N1B4_1391 0.80 -0.4 1,591,517 + -1.4 1,591,518 - +1.7 1,591,518 - +0.7 1,591,518 - +0.1 1,591,518 - +1.1 1,591,519 - -2.2 1,591,524 - +0.9 1,591,524 - +0.1 1,591,680 + +0.1 1,591,681 - -0.3 1,592,247 + Pf1N1B4_1392 0.28 +0.7 1,592,426 - Pf1N1B4_1392 0.35 -1.5 1,592,426 - Pf1N1B4_1392 0.35 -0.9 1,592,524 + Pf1N1B4_1392 0.39 +0.1 1,592,524 + Pf1N1B4_1392 0.39 +1.1 1,592,559 - Pf1N1B4_1392 0.40 +0.0 1,592,559 - Pf1N1B4_1392 0.40 -1.0
Or see this region's nucleotide sequence