Experiment: L-Valine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1229 and Pf1N1B4_1230 are separated by 161 nucleotides Pf1N1B4_1230 and Pf1N1B4_1231 overlap by 4 nucleotides Pf1N1B4_1231 and Pf1N1B4_1232 are separated by 5 nucleotides
Pf1N1B4_1229 - Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27) (from data) , at 1,394,966 to 1,396,483
_1229
Pf1N1B4_1230 - Transcriptional regulator, LysR family, at 1,396,645 to 1,397,592
_1230
Pf1N1B4_1231 - Integral membrane protein YfiB, at 1,397,589 to 1,398,092
_1231
Pf1N1B4_1232 - Inner membrane protein YfiN, at 1,398,098 to 1,399,366
_1232
Position (kb)
1396
1397
1398 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1395.736 kb on + strand at 1395.760 kb on - strand, within Pf1N1B4_1229 at 1395.760 kb on - strand, within Pf1N1B4_1229 at 1396.024 kb on + strand, within Pf1N1B4_1229 at 1396.059 kb on + strand, within Pf1N1B4_1229 at 1396.059 kb on + strand, within Pf1N1B4_1229 at 1396.059 kb on + strand, within Pf1N1B4_1229 at 1396.059 kb on + strand, within Pf1N1B4_1229 at 1396.059 kb on + strand, within Pf1N1B4_1229 at 1396.059 kb on + strand, within Pf1N1B4_1229 at 1396.059 kb on + strand, within Pf1N1B4_1229 at 1396.059 kb on + strand, within Pf1N1B4_1229 at 1396.060 kb on - strand, within Pf1N1B4_1229 at 1396.060 kb on - strand, within Pf1N1B4_1229 at 1396.060 kb on - strand, within Pf1N1B4_1229 at 1396.156 kb on + strand, within Pf1N1B4_1229 at 1396.202 kb on + strand, within Pf1N1B4_1229 at 1396.203 kb on - strand, within Pf1N1B4_1229 at 1396.214 kb on - strand, within Pf1N1B4_1229 at 1396.484 kb on - strand at 1396.506 kb on + strand at 1396.506 kb on + strand at 1396.506 kb on + strand at 1396.506 kb on + strand at 1396.506 kb on + strand at 1396.507 kb on - strand at 1396.507 kb on - strand at 1396.510 kb on + strand at 1396.510 kb on + strand at 1396.511 kb on - strand at 1396.511 kb on - strand at 1396.598 kb on + strand at 1396.598 kb on + strand at 1396.598 kb on + strand at 1396.598 kb on + strand at 1396.598 kb on + strand at 1396.598 kb on + strand at 1396.598 kb on + strand at 1396.598 kb on + strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.614 kb on + strand at 1396.615 kb on - strand at 1396.617 kb on + strand at 1396.629 kb on + strand at 1396.630 kb on - strand at 1396.667 kb on + strand at 1396.759 kb on + strand, within Pf1N1B4_1230 at 1396.759 kb on + strand, within Pf1N1B4_1230 at 1396.759 kb on + strand, within Pf1N1B4_1230 at 1396.760 kb on - strand, within Pf1N1B4_1230 at 1396.760 kb on - strand, within Pf1N1B4_1230 at 1396.760 kb on - strand, within Pf1N1B4_1230 at 1397.131 kb on + strand, within Pf1N1B4_1230 at 1397.131 kb on + strand, within Pf1N1B4_1230 at 1397.131 kb on + strand, within Pf1N1B4_1230 at 1397.132 kb on - strand, within Pf1N1B4_1230 at 1397.132 kb on - strand, within Pf1N1B4_1230 at 1397.170 kb on + strand, within Pf1N1B4_1230 at 1397.170 kb on + strand, within Pf1N1B4_1230 at 1397.170 kb on + strand, within Pf1N1B4_1230 at 1397.170 kb on + strand, within Pf1N1B4_1230 at 1397.171 kb on - strand, within Pf1N1B4_1230 at 1397.248 kb on + strand, within Pf1N1B4_1230 at 1397.248 kb on + strand, within Pf1N1B4_1230 at 1397.248 kb on + strand, within Pf1N1B4_1230 at 1397.248 kb on + strand, within Pf1N1B4_1230 at 1397.248 kb on + strand, within Pf1N1B4_1230 at 1397.249 kb on - strand, within Pf1N1B4_1230 at 1397.249 kb on - strand, within Pf1N1B4_1230 at 1397.249 kb on - strand, within Pf1N1B4_1230 at 1397.249 kb on - strand, within Pf1N1B4_1230 at 1397.284 kb on + strand, within Pf1N1B4_1230 at 1397.284 kb on + strand, within Pf1N1B4_1230 at 1397.285 kb on - strand, within Pf1N1B4_1230 at 1397.467 kb on + strand at 1397.468 kb on - strand, within Pf1N1B4_1230 at 1397.468 kb on - strand, within Pf1N1B4_1230 at 1397.468 kb on - strand, within Pf1N1B4_1230 at 1397.518 kb on + strand at 1397.518 kb on + strand at 1397.666 kb on + strand, within Pf1N1B4_1231 at 1397.667 kb on - strand, within Pf1N1B4_1231 at 1397.706 kb on + strand, within Pf1N1B4_1231 at 1397.706 kb on + strand, within Pf1N1B4_1231 at 1397.706 kb on + strand, within Pf1N1B4_1231 at 1397.707 kb on - strand, within Pf1N1B4_1231 at 1397.707 kb on - strand, within Pf1N1B4_1231 at 1397.768 kb on + strand, within Pf1N1B4_1231 at 1397.770 kb on + strand, within Pf1N1B4_1231 at 1397.770 kb on + strand, within Pf1N1B4_1231 at 1397.770 kb on + strand, within Pf1N1B4_1231 at 1397.770 kb on + strand, within Pf1N1B4_1231 at 1397.770 kb on + strand, within Pf1N1B4_1231 at 1397.770 kb on + strand, within Pf1N1B4_1231 at 1397.770 kb on + strand, within Pf1N1B4_1231 at 1397.770 kb on + strand, within Pf1N1B4_1231 at 1397.771 kb on - strand, within Pf1N1B4_1231 at 1397.771 kb on - strand, within Pf1N1B4_1231 at 1398.066 kb on + strand at 1398.099 kb on + strand at 1398.099 kb on + strand at 1398.099 kb on + strand at 1398.100 kb on - strand at 1398.100 kb on - strand at 1398.180 kb on + strand at 1398.180 kb on + strand at 1398.181 kb on - strand at 1398.352 kb on + strand at 1398.352 kb on + strand, within Pf1N1B4_1232 at 1398.485 kb on - strand, within Pf1N1B4_1232 at 1398.485 kb on - strand, within Pf1N1B4_1232 at 1398.485 kb on - strand, within Pf1N1B4_1232
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (N) remove 1,395,736 + +0.1 1,395,760 - Pf1N1B4_1229 0.52 +0.9 1,395,760 - Pf1N1B4_1229 0.52 +1.1 1,396,024 + Pf1N1B4_1229 0.70 -0.6 1,396,059 + Pf1N1B4_1229 0.72 -1.5 1,396,059 + Pf1N1B4_1229 0.72 -1.5 1,396,059 + Pf1N1B4_1229 0.72 +0.5 1,396,059 + Pf1N1B4_1229 0.72 -0.6 1,396,059 + Pf1N1B4_1229 0.72 +0.7 1,396,059 + Pf1N1B4_1229 0.72 -0.2 1,396,059 + Pf1N1B4_1229 0.72 +0.2 1,396,059 + Pf1N1B4_1229 0.72 +0.2 1,396,060 - Pf1N1B4_1229 0.72 -0.2 1,396,060 - Pf1N1B4_1229 0.72 -0.1 1,396,060 - Pf1N1B4_1229 0.72 -1.3 1,396,156 + Pf1N1B4_1229 0.78 +0.1 1,396,202 + Pf1N1B4_1229 0.81 +2.2 1,396,203 - Pf1N1B4_1229 0.81 +0.1 1,396,214 - Pf1N1B4_1229 0.82 -0.0 1,396,484 - -0.1 1,396,506 + +0.1 1,396,506 + +0.3 1,396,506 + +0.2 1,396,506 + +0.9 1,396,506 + +0.1 1,396,507 - -0.5 1,396,507 - +0.1 1,396,510 + +0.9 1,396,510 + -1.6 1,396,511 - -0.9 1,396,511 - -0.9 1,396,598 + -0.9 1,396,598 + -0.5 1,396,598 + -0.2 1,396,598 + -0.9 1,396,598 + -2.5 1,396,598 + +0.4 1,396,598 + +0.1 1,396,598 + +1.4 1,396,599 - +0.4 1,396,599 - -0.9 1,396,599 - +1.4 1,396,599 - -0.5 1,396,599 - +3.1 1,396,599 - +1.1 1,396,599 - -1.5 1,396,599 - +0.8 1,396,599 - +1.1 1,396,599 - -0.5 1,396,614 + -0.9 1,396,615 - +2.7 1,396,617 + -0.1 1,396,629 + -1.5 1,396,630 - +0.1 1,396,667 + -1.9 1,396,759 + Pf1N1B4_1230 0.12 -1.9 1,396,759 + Pf1N1B4_1230 0.12 +1.7 1,396,759 + Pf1N1B4_1230 0.12 +1.4 1,396,760 - Pf1N1B4_1230 0.12 +0.6 1,396,760 - Pf1N1B4_1230 0.12 -1.6 1,396,760 - Pf1N1B4_1230 0.12 +1.7 1,397,131 + Pf1N1B4_1230 0.51 +2.9 1,397,131 + Pf1N1B4_1230 0.51 -1.3 1,397,131 + Pf1N1B4_1230 0.51 +1.1 1,397,132 - Pf1N1B4_1230 0.51 +1.1 1,397,132 - Pf1N1B4_1230 0.51 +0.4 1,397,170 + Pf1N1B4_1230 0.55 -0.9 1,397,170 + Pf1N1B4_1230 0.55 -1.5 1,397,170 + Pf1N1B4_1230 0.55 -0.9 1,397,170 + Pf1N1B4_1230 0.55 -1.5 1,397,171 - Pf1N1B4_1230 0.55 +0.7 1,397,248 + Pf1N1B4_1230 0.64 +0.1 1,397,248 + Pf1N1B4_1230 0.64 -0.3 1,397,248 + Pf1N1B4_1230 0.64 -1.5 1,397,248 + Pf1N1B4_1230 0.64 -2.3 1,397,248 + Pf1N1B4_1230 0.64 -0.5 1,397,249 - Pf1N1B4_1230 0.64 +0.1 1,397,249 - Pf1N1B4_1230 0.64 -1.5 1,397,249 - Pf1N1B4_1230 0.64 -2.2 1,397,249 - Pf1N1B4_1230 0.64 -0.6 1,397,284 + Pf1N1B4_1230 0.67 +0.1 1,397,284 + Pf1N1B4_1230 0.67 -0.6 1,397,285 - Pf1N1B4_1230 0.68 -0.5 1,397,467 + +0.1 1,397,468 - Pf1N1B4_1230 0.87 -0.6 1,397,468 - Pf1N1B4_1230 0.87 +0.1 1,397,468 - Pf1N1B4_1230 0.87 -2.3 1,397,518 + -0.5 1,397,518 + -0.9 1,397,666 + Pf1N1B4_1231 0.15 -0.9 1,397,667 - Pf1N1B4_1231 0.15 -0.5 1,397,706 + Pf1N1B4_1231 0.23 -1.2 1,397,706 + Pf1N1B4_1231 0.23 +1.3 1,397,706 + Pf1N1B4_1231 0.23 -1.9 1,397,707 - Pf1N1B4_1231 0.23 +0.9 1,397,707 - Pf1N1B4_1231 0.23 +0.9 1,397,768 + Pf1N1B4_1231 0.36 +0.7 1,397,770 + Pf1N1B4_1231 0.36 -0.1 1,397,770 + Pf1N1B4_1231 0.36 +0.8 1,397,770 + Pf1N1B4_1231 0.36 +0.1 1,397,770 + Pf1N1B4_1231 0.36 +0.1 1,397,770 + Pf1N1B4_1231 0.36 -0.1 1,397,770 + Pf1N1B4_1231 0.36 -0.9 1,397,770 + Pf1N1B4_1231 0.36 +1.0 1,397,770 + Pf1N1B4_1231 0.36 +1.5 1,397,771 - Pf1N1B4_1231 0.36 -0.5 1,397,771 - Pf1N1B4_1231 0.36 +1.0 1,398,066 + +0.1 1,398,099 + +1.3 1,398,099 + +0.5 1,398,099 + -0.6 1,398,100 - +0.4 1,398,100 - +1.1 1,398,180 + +0.4 1,398,180 + +0.4 1,398,181 - +1.7 1,398,352 + +0.1 1,398,352 + Pf1N1B4_1232 0.20 -0.6 1,398,485 - Pf1N1B4_1232 0.30 -0.6 1,398,485 - Pf1N1B4_1232 0.30 -0.5 1,398,485 - Pf1N1B4_1232 0.30 +0.5
Or see this region's nucleotide sequence