Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_05722

Experiment: 2-Deoxy-D-Ribose 10 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_05720 and H281DRAFT_05721 are separated by 201 nucleotidesH281DRAFT_05721 and H281DRAFT_05722 are separated by 1 nucleotidesH281DRAFT_05722 and H281DRAFT_05723 are separated by 53 nucleotides H281DRAFT_05720: H281DRAFT_05720 - phenylacetate-CoA ligase, at 139,050 to 140,354 _05720 H281DRAFT_05721: H281DRAFT_05721 - acyl-CoA thioesterase, at 140,556 to 141,044 _05721 H281DRAFT_05722: H281DRAFT_05722 - 1,2-epoxyphenylacetyl-CoA isomerase (EC 5.3.3.18) (from data), at 141,046 to 141,837 _05722 H281DRAFT_05723: H281DRAFT_05723 - bifunctional thiolase PaaJ: 3-oxo-5,6-didehydrosuberyl-CoA thiolase (EC 2.3.1.223); 3-oxoadipyl-CoA thiolase (EC 2.3.1.174) (from data), at 141,891 to 143,093 _05723 Position (kb) 141 142Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 140.131 kb on + strandat 140.236 kb on + strandat 140.237 kb on - strandat 140.255 kb on - strandat 140.301 kb on + strandat 140.301 kb on + strandat 140.302 kb on - strandat 140.302 kb on - strandat 140.391 kb on + strandat 140.557 kb on + strandat 140.558 kb on - strandat 140.558 kb on - strandat 140.648 kb on + strand, within H281DRAFT_05721at 140.663 kb on + strand, within H281DRAFT_05721at 140.663 kb on + strand, within H281DRAFT_05721at 140.664 kb on - strand, within H281DRAFT_05721at 140.664 kb on - strand, within H281DRAFT_05721at 140.664 kb on - strand, within H281DRAFT_05721at 140.664 kb on - strand, within H281DRAFT_05721at 140.667 kb on + strand, within H281DRAFT_05721at 140.668 kb on - strand, within H281DRAFT_05721at 140.676 kb on - strand, within H281DRAFT_05721at 140.680 kb on + strand, within H281DRAFT_05721at 140.680 kb on + strand, within H281DRAFT_05721at 140.680 kb on + strand, within H281DRAFT_05721at 140.681 kb on - strand, within H281DRAFT_05721at 140.681 kb on - strand, within H281DRAFT_05721at 140.797 kb on + strand, within H281DRAFT_05721at 140.798 kb on - strand, within H281DRAFT_05721at 140.798 kb on - strand, within H281DRAFT_05721at 140.798 kb on - strand, within H281DRAFT_05721at 140.837 kb on + strand, within H281DRAFT_05721at 140.837 kb on + strand, within H281DRAFT_05721at 140.838 kb on - strand, within H281DRAFT_05721at 140.841 kb on + strand, within H281DRAFT_05721at 140.912 kb on - strand, within H281DRAFT_05721at 140.972 kb on - strand, within H281DRAFT_05721at 140.972 kb on - strand, within H281DRAFT_05721at 141.025 kb on + strandat 141.025 kb on + strandat 141.044 kb on + strandat 141.045 kb on - strandat 141.045 kb on - strandat 141.045 kb on - strandat 141.104 kb on + strandat 141.104 kb on + strandat 141.104 kb on + strandat 141.199 kb on - strand, within H281DRAFT_05722at 141.199 kb on - strand, within H281DRAFT_05722at 141.199 kb on - strand, within H281DRAFT_05722at 141.295 kb on - strand, within H281DRAFT_05722at 141.295 kb on - strand, within H281DRAFT_05722at 141.295 kb on - strand, within H281DRAFT_05722at 141.295 kb on - strand, within H281DRAFT_05722at 141.295 kb on - strand, within H281DRAFT_05722at 141.295 kb on - strand, within H281DRAFT_05722at 141.295 kb on - strand, within H281DRAFT_05722at 141.353 kb on - strand, within H281DRAFT_05722at 141.527 kb on - strand, within H281DRAFT_05722at 141.791 kb on - strandat 141.830 kb on + strandat 141.880 kb on + strandat 141.880 kb on + strandat 141.880 kb on + strandat 141.881 kb on - strandat 141.884 kb on + strandat 141.884 kb on + strandat 141.884 kb on + strandat 141.884 kb on + strandat 141.885 kb on - strandat 141.885 kb on - strandat 141.885 kb on - strandat 141.907 kb on - strandat 142.276 kb on + strand, within H281DRAFT_05723at 142.276 kb on + strand, within H281DRAFT_05723at 142.353 kb on + strand, within H281DRAFT_05723at 142.354 kb on - strand, within H281DRAFT_05723at 142.374 kb on + strand, within H281DRAFT_05723at 142.374 kb on + strand, within H281DRAFT_05723at 142.374 kb on + strand, within H281DRAFT_05723at 142.375 kb on - strand, within H281DRAFT_05723at 142.375 kb on - strand, within H281DRAFT_05723at 142.375 kb on - strand, within H281DRAFT_05723at 142.375 kb on - strand, within H281DRAFT_05723at 142.375 kb on - strand, within H281DRAFT_05723at 142.837 kb on - strand, within H281DRAFT_05723

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxy-D-Ribose 10 mM (C)
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140,131 + +1.3
140,236 + -0.2
140,237 - +1.1
140,255 - +0.2
140,301 + -0.3
140,301 + +2.3
140,302 - +0.1
140,302 - -0.4
140,391 + +0.3
140,557 + -1.1
140,558 - +1.3
140,558 - +0.7
140,648 + H281DRAFT_05721 0.19 +0.8
140,663 + H281DRAFT_05721 0.22 +0.2
140,663 + H281DRAFT_05721 0.22 +0.5
140,664 - H281DRAFT_05721 0.22 +2.5
140,664 - H281DRAFT_05721 0.22 +0.4
140,664 - H281DRAFT_05721 0.22 +0.4
140,664 - H281DRAFT_05721 0.22 -0.0
140,667 + H281DRAFT_05721 0.23 -0.3
140,668 - H281DRAFT_05721 0.23 +0.2
140,676 - H281DRAFT_05721 0.25 -0.4
140,680 + H281DRAFT_05721 0.25 +1.6
140,680 + H281DRAFT_05721 0.25 +0.5
140,680 + H281DRAFT_05721 0.25 +0.2
140,681 - H281DRAFT_05721 0.26 -0.4
140,681 - H281DRAFT_05721 0.26 +0.3
140,797 + H281DRAFT_05721 0.49 +3.9
140,798 - H281DRAFT_05721 0.49 +0.9
140,798 - H281DRAFT_05721 0.49 +1.3
140,798 - H281DRAFT_05721 0.49 +0.1
140,837 + H281DRAFT_05721 0.57 +0.7
140,837 + H281DRAFT_05721 0.57 +0.5
140,838 - H281DRAFT_05721 0.58 +0.7
140,841 + H281DRAFT_05721 0.58 +0.0
140,912 - H281DRAFT_05721 0.73 +2.1
140,972 - H281DRAFT_05721 0.85 +0.5
140,972 - H281DRAFT_05721 0.85 +0.6
141,025 + +0.3
141,025 + +0.2
141,044 + +0.1
141,045 - +0.1
141,045 - +1.1
141,045 - +1.0
141,104 + -0.2
141,104 + +1.5
141,104 + +0.8
141,199 - H281DRAFT_05722 0.19 -0.3
141,199 - H281DRAFT_05722 0.19 -0.2
141,199 - H281DRAFT_05722 0.19 -0.6
141,295 - H281DRAFT_05722 0.31 +0.8
141,295 - H281DRAFT_05722 0.31 -2.2
141,295 - H281DRAFT_05722 0.31 +1.2
141,295 - H281DRAFT_05722 0.31 +0.7
141,295 - H281DRAFT_05722 0.31 +0.7
141,295 - H281DRAFT_05722 0.31 +0.4
141,295 - H281DRAFT_05722 0.31 +0.0
141,353 - H281DRAFT_05722 0.39 -0.7
141,527 - H281DRAFT_05722 0.61 +2.0
141,791 - -0.5
141,830 + +0.3
141,880 + +0.3
141,880 + -0.3
141,880 + +0.8
141,881 - +0.8
141,884 + +0.5
141,884 + +0.3
141,884 + +0.1
141,884 + -0.0
141,885 - +0.9
141,885 - +0.4
141,885 - -0.9
141,907 - -0.5
142,276 + H281DRAFT_05723 0.32 +0.7
142,276 + H281DRAFT_05723 0.32 +2.3
142,353 + H281DRAFT_05723 0.38 +0.2
142,354 - H281DRAFT_05723 0.38 -0.4
142,374 + H281DRAFT_05723 0.40 -0.1
142,374 + H281DRAFT_05723 0.40 +1.8
142,374 + H281DRAFT_05723 0.40 +1.1
142,375 - H281DRAFT_05723 0.40 +0.3
142,375 - H281DRAFT_05723 0.40 -0.3
142,375 - H281DRAFT_05723 0.40 -0.4
142,375 - H281DRAFT_05723 0.40 +0.7
142,375 - H281DRAFT_05723 0.40 -0.0
142,837 - H281DRAFT_05723 0.79 -0.3

Or see this region's nucleotide sequence