Experiment: incubated with with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate for 3 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3104 and Dsui_3105 are separated by 54 nucleotides Dsui_3105 and Dsui_3106 are separated by 291 nucleotides Dsui_3106 and Dsui_3107 overlap by 8 nucleotides
Dsui_3104: Dsui_3104 - PAS domain S-box/diguanylate cyclase (GGDEF) domain-containing protein, at 3,322,174 to 3,324,675
_3104
Dsui_3105: Dsui_3105 - CBS domain-containing protein, at 3,324,730 to 3,326,046
_3105
Dsui_3106: Dsui_3106 - hypothetical protein, at 3,326,338 to 3,326,718
_3106
Dsui_3107: Dsui_3107 - Zn-dependent hydrolase, glyoxylase, at 3,326,711 to 3,327,349
_3107
Position (kb)
3324
3325
3326
3327 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3323.749 kb on + strand, within Dsui_3104 at 3323.817 kb on - strand, within Dsui_3104 at 3323.825 kb on - strand, within Dsui_3104 at 3324.226 kb on - strand, within Dsui_3104 at 3324.242 kb on + strand, within Dsui_3104 at 3324.295 kb on - strand, within Dsui_3104 at 3324.302 kb on - strand, within Dsui_3104 at 3324.375 kb on - strand, within Dsui_3104 at 3324.432 kb on + strand at 3324.432 kb on + strand at 3324.440 kb on - strand at 3324.523 kb on - strand at 3324.609 kb on - strand at 3324.700 kb on + strand at 3324.871 kb on - strand, within Dsui_3105 at 3324.943 kb on - strand, within Dsui_3105 at 3325.164 kb on + strand, within Dsui_3105 at 3325.172 kb on - strand, within Dsui_3105 at 3325.175 kb on - strand, within Dsui_3105 at 3325.245 kb on - strand, within Dsui_3105 at 3325.247 kb on + strand, within Dsui_3105 at 3325.255 kb on - strand, within Dsui_3105 at 3325.255 kb on - strand, within Dsui_3105 at 3325.255 kb on - strand, within Dsui_3105 at 3325.257 kb on + strand, within Dsui_3105 at 3325.534 kb on - strand, within Dsui_3105 at 3325.736 kb on + strand, within Dsui_3105 at 3325.744 kb on - strand, within Dsui_3105 at 3325.791 kb on + strand, within Dsui_3105 at 3325.798 kb on + strand, within Dsui_3105 at 3325.799 kb on - strand, within Dsui_3105 at 3326.076 kb on + strand at 3326.084 kb on - strand at 3326.084 kb on - strand at 3326.168 kb on - strand at 3326.227 kb on - strand at 3326.270 kb on - strand at 3326.344 kb on - strand at 3326.513 kb on - strand, within Dsui_3106 at 3326.572 kb on + strand, within Dsui_3106 at 3326.581 kb on + strand at 3326.647 kb on - strand, within Dsui_3106 at 3326.903 kb on - strand, within Dsui_3107 at 3326.908 kb on + strand at 3326.912 kb on - strand, within Dsui_3107 at 3326.974 kb on - strand, within Dsui_3107 at 3327.039 kb on - strand, within Dsui_3107
Per-strain Table
Position Strand Gene LocusTag Fraction incubated with with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate for 3 hours remove 3,323,749 + Dsui_3104 0.63 +2.0 3,323,817 - Dsui_3104 0.66 +0.4 3,323,825 - Dsui_3104 0.66 +0.4 3,324,226 - Dsui_3104 0.82 +1.5 3,324,242 + Dsui_3104 0.83 +1.0 3,324,295 - Dsui_3104 0.85 +2.0 3,324,302 - Dsui_3104 0.85 +1.4 3,324,375 - Dsui_3104 0.88 +1.9 3,324,432 + +1.1 3,324,432 + +1.7 3,324,440 - +1.2 3,324,523 - +0.8 3,324,609 - +0.6 3,324,700 + +1.0 3,324,871 - Dsui_3105 0.11 +0.6 3,324,943 - Dsui_3105 0.16 -0.1 3,325,164 + Dsui_3105 0.33 -0.3 3,325,172 - Dsui_3105 0.34 -0.2 3,325,175 - Dsui_3105 0.34 +1.1 3,325,245 - Dsui_3105 0.39 +1.7 3,325,247 + Dsui_3105 0.39 -3.5 3,325,255 - Dsui_3105 0.40 +0.4 3,325,255 - Dsui_3105 0.40 +0.3 3,325,255 - Dsui_3105 0.40 -3.5 3,325,257 + Dsui_3105 0.40 +0.5 3,325,534 - Dsui_3105 0.61 +1.3 3,325,736 + Dsui_3105 0.76 +1.2 3,325,744 - Dsui_3105 0.77 -0.5 3,325,791 + Dsui_3105 0.81 -0.9 3,325,798 + Dsui_3105 0.81 -0.3 3,325,799 - Dsui_3105 0.81 +0.1 3,326,076 + -1.6 3,326,084 - +1.2 3,326,084 - +0.1 3,326,168 - +1.0 3,326,227 - +2.1 3,326,270 - +1.1 3,326,344 - -0.1 3,326,513 - Dsui_3106 0.46 +1.0 3,326,572 + Dsui_3106 0.61 -1.4 3,326,581 + -3.5 3,326,647 - Dsui_3106 0.81 +0.9 3,326,903 - Dsui_3107 0.30 +0.4 3,326,908 + -4.4 3,326,912 - Dsui_3107 0.31 -0.8 3,326,974 - Dsui_3107 0.41 -0.8 3,327,039 - Dsui_3107 0.51 -0.5
Or see this region's nucleotide sequence