Experiment: incubated with with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate for 3 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2149 and Dsui_2150 are separated by 4 nucleotides Dsui_2150 and Dsui_2151 overlap by 1 nucleotides Dsui_2151 and Dsui_2152 are separated by 30 nucleotides
Dsui_2149: Dsui_2149 - DNA helicase, Rad3, at 2,278,825 to 2,280,777
_2149
Dsui_2150: Dsui_2150 - hypothetical protein, at 2,280,782 to 2,282,134
_2150
Dsui_2151: Dsui_2151 - molybdopterin-guanine dinucleotide biosynthesis protein MobB, at 2,282,134 to 2,282,655
_2151
Dsui_2152: Dsui_2152 - molybdenum cofactor synthesis domain protein, at 2,282,686 to 2,283,912
_2152
Position (kb)
2280
2281
2282
2283 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2279.805 kb on + strand, within Dsui_2149 at 2279.805 kb on + strand, within Dsui_2149 at 2279.805 kb on + strand, within Dsui_2149 at 2279.848 kb on - strand at 2279.918 kb on + strand, within Dsui_2149 at 2279.918 kb on + strand, within Dsui_2149 at 2279.925 kb on + strand, within Dsui_2149 at 2280.019 kb on - strand at 2280.153 kb on - strand at 2280.353 kb on - strand, within Dsui_2149 at 2280.464 kb on + strand, within Dsui_2149 at 2280.576 kb on + strand, within Dsui_2149 at 2280.654 kb on - strand at 2280.786 kb on - strand at 2280.868 kb on - strand at 2280.870 kb on + strand at 2280.881 kb on + strand at 2280.881 kb on + strand at 2280.889 kb on - strand at 2281.146 kb on + strand, within Dsui_2150 at 2281.382 kb on + strand, within Dsui_2150 at 2281.490 kb on + strand, within Dsui_2150 at 2281.604 kb on - strand, within Dsui_2150 at 2281.673 kb on - strand at 2281.924 kb on - strand, within Dsui_2150 at 2281.986 kb on + strand, within Dsui_2150 at 2282.126 kb on - strand at 2282.195 kb on + strand, within Dsui_2151 at 2282.261 kb on + strand, within Dsui_2151 at 2282.269 kb on - strand, within Dsui_2151 at 2282.284 kb on - strand, within Dsui_2151 at 2282.517 kb on + strand, within Dsui_2151 at 2282.581 kb on - strand, within Dsui_2151 at 2282.655 kb on - strand at 2282.763 kb on - strand at 2282.844 kb on + strand, within Dsui_2152 at 2282.933 kb on + strand, within Dsui_2152 at 2282.941 kb on - strand, within Dsui_2152 at 2282.977 kb on + strand, within Dsui_2152 at 2283.070 kb on - strand, within Dsui_2152
Per-strain Table
Position Strand Gene LocusTag Fraction incubated with with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate for 3 hours remove 2,279,805 + Dsui_2149 0.50 +0.2 2,279,805 + Dsui_2149 0.50 -0.1 2,279,805 + Dsui_2149 0.50 -0.3 2,279,848 - -2.2 2,279,918 + Dsui_2149 0.56 -5.6 2,279,918 + Dsui_2149 0.56 -2.0 2,279,925 + Dsui_2149 0.56 -1.3 2,280,019 - -1.5 2,280,153 - +0.1 2,280,353 - Dsui_2149 0.78 -4.5 2,280,464 + Dsui_2149 0.84 -0.8 2,280,576 + Dsui_2149 0.90 +1.3 2,280,654 - -2.1 2,280,786 - -2.7 2,280,868 - -1.2 2,280,870 + -6.3 2,280,881 + -2.7 2,280,881 + -3.3 2,280,889 - -4.2 2,281,146 + Dsui_2150 0.27 -6.4 2,281,382 + Dsui_2150 0.44 -4.4 2,281,490 + Dsui_2150 0.52 -4.0 2,281,604 - Dsui_2150 0.61 -4.7 2,281,673 - -2.6 2,281,924 - Dsui_2150 0.84 -4.6 2,281,986 + Dsui_2150 0.89 -0.8 2,282,126 - -2.7 2,282,195 + Dsui_2151 0.12 -3.9 2,282,261 + Dsui_2151 0.24 -5.6 2,282,269 - Dsui_2151 0.26 -4.3 2,282,284 - Dsui_2151 0.29 -4.9 2,282,517 + Dsui_2151 0.73 -4.3 2,282,581 - Dsui_2151 0.86 -3.4 2,282,655 - -2.6 2,282,763 - -1.5 2,282,844 + Dsui_2152 0.13 -3.9 2,282,933 + Dsui_2152 0.20 -4.1 2,282,941 - Dsui_2152 0.21 -3.7 2,282,977 + Dsui_2152 0.24 -2.9 2,283,070 - Dsui_2152 0.31 -3.8
Or see this region's nucleotide sequence