Experiment: incubated with with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate for 3 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1401 and Dsui_1402 are separated by 97 nucleotides Dsui_1402 and Dsui_1403 overlap by 1 nucleotides Dsui_1403 and Dsui_1404 are separated by 19 nucleotides Dsui_1404 and Dsui_1405 overlap by 4 nucleotides
Dsui_1401: Dsui_1401 - surface lipoprotein, at 1,461,079 to 1,461,798
_1401
Dsui_1402: Dsui_1402 - ABC-type transport system involved in resistance to organic solvents, auxiliary component, at 1,461,896 to 1,462,525
_1402
Dsui_1403: Dsui_1403 - putative NTP binding protein (contains STAS domain), at 1,462,525 to 1,462,812
_1403
Dsui_1404: Dsui_1404 - ABC-type multidrug transport system, ATPase component, at 1,462,832 to 1,463,755
_1404
Dsui_1405: Dsui_1405 - ABC-type polysaccharide/polyol phosphate export systems, permease component, at 1,463,752 to 1,464,507
_1405
Position (kb)
1462
1463 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1461.637 kb on - strand at 1461.879 kb on + strand at 1462.045 kb on + strand, within Dsui_1402 at 1462.141 kb on + strand, within Dsui_1402 at 1462.336 kb on + strand, within Dsui_1402 at 1462.514 kb on + strand at 1462.562 kb on + strand, within Dsui_1403 at 1462.806 kb on + strand at 1462.807 kb on + strand at 1462.990 kb on + strand, within Dsui_1404 at 1463.451 kb on + strand, within Dsui_1404
Per-strain Table
Position Strand Gene LocusTag Fraction incubated with with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate for 3 hours remove 1,461,637 - -1.8 1,461,879 + -1.3 1,462,045 + Dsui_1402 0.24 -4.0 1,462,141 + Dsui_1402 0.39 -2.8 1,462,336 + Dsui_1402 0.70 -2.8 1,462,514 + +0.1 1,462,562 + Dsui_1403 0.13 -4.5 1,462,806 + +0.7 1,462,807 + +0.8 1,462,990 + Dsui_1404 0.17 +0.5 1,463,451 + Dsui_1404 0.67 +0.1
Or see this region's nucleotide sequence