Experiment: incubated with with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate for 3 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0731 and Dsui_0732 are separated by 12 nucleotides Dsui_0732 and Dsui_0733 are separated by 12 nucleotides Dsui_0733 and Dsui_0734 overlap by 4 nucleotides Dsui_0734 and Dsui_0735 are separated by 265 nucleotides Dsui_0735 and Dsui_0736 are separated by 93 nucleotides
Dsui_0731: Dsui_0731 - microcystin-dependent protein, at 729,312 to 729,833
_0731
Dsui_0732: Dsui_0732 - microcystin-dependent protein, at 729,846 to 730,379
_0732
Dsui_0733: Dsui_0733 - hypothetical protein, at 730,392 to 730,703
_0733
Dsui_0734: Dsui_0734 - acetyltransferase, at 730,700 to 731,239
_0734
Dsui_0735: Dsui_0735 - putative membrane protein, at 731,505 to 731,984
_0735
Dsui_0736: Dsui_0736 - FAD/FMN-dependent dehydrogenase, at 732,078 to 735,956
_0736
Position (kb)
730
731
732 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 729.728 kb on - strand, within Dsui_0731 at 729.760 kb on - strand, within Dsui_0731 at 729.772 kb on + strand, within Dsui_0731 at 729.777 kb on + strand, within Dsui_0731 at 729.837 kb on + strand at 729.906 kb on - strand, within Dsui_0732 at 730.012 kb on + strand, within Dsui_0732 at 730.020 kb on - strand, within Dsui_0732 at 730.097 kb on + strand, within Dsui_0732 at 730.131 kb on + strand at 730.134 kb on - strand, within Dsui_0732 at 730.152 kb on + strand, within Dsui_0732 at 730.152 kb on + strand, within Dsui_0732 at 730.160 kb on - strand, within Dsui_0732 at 730.196 kb on - strand, within Dsui_0732 at 730.204 kb on - strand, within Dsui_0732 at 730.211 kb on + strand, within Dsui_0732 at 730.219 kb on - strand, within Dsui_0732 at 730.223 kb on - strand, within Dsui_0732 at 730.316 kb on + strand, within Dsui_0732 at 730.316 kb on + strand at 730.318 kb on + strand, within Dsui_0732 at 730.324 kb on - strand, within Dsui_0732 at 730.324 kb on - strand, within Dsui_0732 at 730.324 kb on - strand, within Dsui_0732 at 730.325 kb on - strand, within Dsui_0732 at 730.333 kb on + strand at 730.398 kb on - strand at 730.498 kb on - strand, within Dsui_0733 at 730.524 kb on - strand, within Dsui_0733 at 730.551 kb on + strand, within Dsui_0733 at 730.559 kb on - strand, within Dsui_0733 at 730.607 kb on - strand, within Dsui_0733 at 730.644 kb on + strand, within Dsui_0733 at 730.657 kb on + strand at 730.707 kb on + strand at 730.707 kb on + strand at 730.728 kb on + strand at 730.865 kb on - strand, within Dsui_0734 at 731.030 kb on - strand, within Dsui_0734 at 731.080 kb on - strand, within Dsui_0734 at 731.202 kb on - strand at 731.202 kb on - strand at 731.242 kb on - strand at 731.439 kb on + strand at 731.441 kb on - strand at 731.441 kb on - strand at 731.451 kb on - strand at 731.458 kb on + strand at 731.458 kb on + strand at 731.466 kb on - strand at 731.466 kb on - strand at 731.466 kb on - strand at 731.487 kb on + strand at 731.493 kb on + strand at 731.495 kb on - strand at 731.514 kb on + strand at 731.527 kb on - strand at 731.527 kb on - strand at 731.527 kb on - strand at 731.540 kb on - strand at 731.556 kb on - strand, within Dsui_0735 at 731.567 kb on - strand, within Dsui_0735 at 731.663 kb on - strand, within Dsui_0735 at 731.675 kb on - strand, within Dsui_0735 at 731.749 kb on - strand, within Dsui_0735 at 731.802 kb on - strand, within Dsui_0735 at 731.821 kb on + strand, within Dsui_0735 at 731.835 kb on - strand, within Dsui_0735 at 731.913 kb on - strand, within Dsui_0735 at 731.915 kb on - strand, within Dsui_0735 at 732.000 kb on + strand at 732.007 kb on + strand at 732.008 kb on - strand at 732.014 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction incubated with with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate for 3 hours remove 729,728 - Dsui_0731 0.80 -2.7 729,760 - Dsui_0731 0.86 -1.1 729,772 + Dsui_0731 0.88 -0.1 729,777 + Dsui_0731 0.89 -0.4 729,837 + -0.6 729,906 - Dsui_0732 0.11 +1.2 730,012 + Dsui_0732 0.31 -0.3 730,020 - Dsui_0732 0.33 +0.2 730,097 + Dsui_0732 0.47 -1.5 730,131 + -2.3 730,134 - Dsui_0732 0.54 +0.5 730,152 + Dsui_0732 0.57 +0.7 730,152 + Dsui_0732 0.57 -0.9 730,160 - Dsui_0732 0.59 +1.3 730,196 - Dsui_0732 0.66 -3.6 730,204 - Dsui_0732 0.67 -0.5 730,211 + Dsui_0732 0.68 +2.2 730,219 - Dsui_0732 0.70 +0.8 730,223 - Dsui_0732 0.71 +0.3 730,316 + Dsui_0732 0.88 -1.0 730,316 + -3.6 730,318 + Dsui_0732 0.88 +0.1 730,324 - Dsui_0732 0.90 -0.0 730,324 - Dsui_0732 0.90 -0.8 730,324 - Dsui_0732 0.90 -1.2 730,325 - Dsui_0732 0.90 -0.5 730,333 + +0.9 730,398 - +0.0 730,498 - Dsui_0733 0.34 -1.2 730,524 - Dsui_0733 0.42 -0.4 730,551 + Dsui_0733 0.51 -3.1 730,559 - Dsui_0733 0.54 +0.6 730,607 - Dsui_0733 0.69 -2.8 730,644 + Dsui_0733 0.81 +0.0 730,657 + -2.2 730,707 + -0.8 730,707 + -0.6 730,728 + -0.0 730,865 - Dsui_0734 0.31 -1.0 731,030 - Dsui_0734 0.61 -2.1 731,080 - Dsui_0734 0.70 -1.4 731,202 - +1.1 731,202 - +0.1 731,242 - +0.2 731,439 + -1.1 731,441 - -0.9 731,441 - -0.8 731,451 - -0.5 731,458 + +2.3 731,458 + -2.4 731,466 - +0.5 731,466 - +0.2 731,466 - -0.5 731,487 + -0.4 731,493 + -0.3 731,495 - +1.3 731,514 + +0.5 731,527 - -1.2 731,527 - -0.6 731,527 - -2.1 731,540 - -4.8 731,556 - Dsui_0735 0.11 -2.6 731,567 - Dsui_0735 0.13 -0.5 731,663 - Dsui_0735 0.33 -1.4 731,675 - Dsui_0735 0.35 -0.7 731,749 - Dsui_0735 0.51 -0.2 731,802 - Dsui_0735 0.62 -0.4 731,821 + Dsui_0735 0.66 +0.7 731,835 - Dsui_0735 0.69 +0.0 731,913 - Dsui_0735 0.85 -0.9 731,915 - Dsui_0735 0.85 -0.6 732,000 + +1.2 732,007 + +0.7 732,008 - +0.4 732,014 - -0.8
Or see this region's nucleotide sequence