Experiment: incubated with with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate for 3 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0538 and Dsui_0539 are separated by 9 nucleotides Dsui_0539 and Dsui_0540 overlap by 4 nucleotides Dsui_0540 and Dsui_0541 overlap by 8 nucleotides
Dsui_0538: Dsui_0538 - hypothetical protein, at 542,492 to 543,235
_0538
Dsui_0539: Dsui_0539 - ABC-type Fe3+-hydroxamate transport system, periplasmic component, at 543,245 to 544,129
_0539
Dsui_0540: Dsui_0540 - L-threonine-O-3-phosphate decarboxylase, at 544,126 to 545,130
_0540
Dsui_0541: Dsui_0541 - cobalamin biosynthesis protein CobD, at 545,123 to 546,094
_0541
Position (kb) 544
545
546 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 543.273 kb on + strand at 543.295 kb on + strand at 543.351 kb on + strand, within Dsui_0539 at 543.375 kb on + strand, within Dsui_0539 at 543.609 kb on - strand, within Dsui_0539 at 543.609 kb on - strand, within Dsui_0539 at 543.615 kb on + strand, within Dsui_0539 at 543.615 kb on + strand, within Dsui_0539 at 543.623 kb on - strand, within Dsui_0539 at 543.750 kb on + strand at 543.786 kb on - strand, within Dsui_0539 at 543.788 kb on + strand, within Dsui_0539 at 543.830 kb on + strand, within Dsui_0539 at 543.952 kb on - strand, within Dsui_0539 at 544.214 kb on - strand at 544.329 kb on + strand, within Dsui_0540 at 544.337 kb on - strand, within Dsui_0540 at 544.352 kb on + strand at 544.484 kb on + strand, within Dsui_0540 at 544.526 kb on + strand, within Dsui_0540 at 544.556 kb on - strand, within Dsui_0540 at 544.672 kb on - strand, within Dsui_0540 at 544.937 kb on - strand, within Dsui_0540 at 544.965 kb on + strand, within Dsui_0540 at 544.965 kb on + strand at 544.973 kb on - strand, within Dsui_0540 at 545.035 kb on - strand at 545.537 kb on - strand, within Dsui_0541 at 545.556 kb on + strand, within Dsui_0541 at 545.564 kb on - strand, within Dsui_0541 at 545.612 kb on - strand, within Dsui_0541 at 545.612 kb on - strand, within Dsui_0541 at 545.650 kb on - strand, within Dsui_0541 at 545.671 kb on - strand, within Dsui_0541 at 545.671 kb on - strand, within Dsui_0541 at 545.671 kb on - strand, within Dsui_0541 at 545.682 kb on - strand, within Dsui_0541 at 545.682 kb on - strand, within Dsui_0541 at 545.697 kb on - strand, within Dsui_0541 at 545.733 kb on + strand at 545.738 kb on + strand, within Dsui_0541 at 545.799 kb on - strand, within Dsui_0541 at 545.861 kb on + strand at 545.886 kb on - strand, within Dsui_0541 at 545.922 kb on - strand, within Dsui_0541 at 546.037 kb on - strand at 546.076 kb on + strand at 546.079 kb on - strand at 546.084 kb on - strand at 546.084 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction incubated with with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate for 3 hours remove 543,273 + +1.1 543,295 + +0.9 543,351 + Dsui_0539 0.12 -1.3 543,375 + Dsui_0539 0.15 +0.2 543,609 - Dsui_0539 0.41 +0.6 543,609 - Dsui_0539 0.41 +0.4 543,615 + Dsui_0539 0.42 +0.3 543,615 + Dsui_0539 0.42 -0.9 543,623 - Dsui_0539 0.43 +0.6 543,750 + -1.4 543,786 - Dsui_0539 0.61 +0.3 543,788 + Dsui_0539 0.61 -0.3 543,830 + Dsui_0539 0.66 -0.6 543,952 - Dsui_0539 0.80 +1.2 544,214 - +0.2 544,329 + Dsui_0540 0.20 +1.1 544,337 - Dsui_0540 0.21 -0.8 544,352 + -1.1 544,484 + Dsui_0540 0.36 -2.3 544,526 + Dsui_0540 0.40 -1.8 544,556 - Dsui_0540 0.43 -0.0 544,672 - Dsui_0540 0.54 -0.3 544,937 - Dsui_0540 0.81 -3.0 544,965 + Dsui_0540 0.83 -1.3 544,965 + +0.6 544,973 - Dsui_0540 0.84 +0.5 545,035 - +0.4 545,537 - Dsui_0541 0.43 +0.1 545,556 + Dsui_0541 0.45 -1.2 545,564 - Dsui_0541 0.45 +0.2 545,612 - Dsui_0541 0.50 +0.9 545,612 - Dsui_0541 0.50 +1.3 545,650 - Dsui_0541 0.54 -1.0 545,671 - Dsui_0541 0.56 +0.5 545,671 - Dsui_0541 0.56 -1.3 545,671 - Dsui_0541 0.56 +0.7 545,682 - Dsui_0541 0.58 -0.5 545,682 - Dsui_0541 0.58 -3.6 545,697 - Dsui_0541 0.59 -1.1 545,733 + -2.0 545,738 + Dsui_0541 0.63 -0.1 545,799 - Dsui_0541 0.70 -0.4 545,861 + -0.4 545,886 - Dsui_0541 0.78 -0.6 545,922 - Dsui_0541 0.82 +0.5 546,037 - -0.0 546,076 + -1.1 546,079 - +0.7 546,084 - +0.5 546,084 - +1.2
Or see this region's nucleotide sequence