Experiment: incubated with with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate for 3 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0470 and Dsui_0471 overlap by 16 nucleotides Dsui_0471 and Dsui_0472 are separated by 10 nucleotides Dsui_0472 and Dsui_0473 are separated by 83 nucleotides Dsui_0473 and Dsui_0474 are separated by 83 nucleotides
Dsui_0470: Dsui_0470 - fructose-2,6-bisphosphatase, at 477,597 to 478,166
_0470
Dsui_0471: Dsui_0471 - cobalamin 5''-phosphate synthase/cobalamin synthase, at 478,151 to 478,927
_0471
Dsui_0472: Dsui_0472 - nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase, at 478,938 to 479,993
_0472
Dsui_0473: Dsui_0473 - adenosyl cobinamide kinase/adenosyl cobinamide phosphate guanylyltransferase, at 480,077 to 480,655
_0473
Dsui_0474: Dsui_0474 - universal stress protein UspA-like protein, at 480,739 to 481,191
_0474
Position (kb) 478
479
480 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 477.995 kb on + strand, within Dsui_0470 at 478.134 kb on + strand at 478.169 kb on - strand at 478.169 kb on - strand at 478.199 kb on + strand at 478.325 kb on - strand, within Dsui_0471 at 478.341 kb on + strand at 478.442 kb on - strand, within Dsui_0471 at 478.583 kb on - strand, within Dsui_0471 at 478.720 kb on + strand at 478.737 kb on + strand, within Dsui_0471 at 478.943 kb on - strand at 478.943 kb on - strand at 478.992 kb on - strand at 479.080 kb on - strand, within Dsui_0472 at 479.138 kb on - strand, within Dsui_0472 at 479.251 kb on + strand at 479.283 kb on - strand, within Dsui_0472 at 479.335 kb on + strand at 479.343 kb on - strand, within Dsui_0472 at 479.582 kb on + strand, within Dsui_0472 at 479.678 kb on + strand at 479.678 kb on + strand, within Dsui_0472 at 479.752 kb on + strand, within Dsui_0472 at 479.891 kb on + strand at 479.908 kb on + strand at 479.908 kb on + strand at 479.908 kb on + strand at 479.949 kb on + strand at 479.972 kb on + strand at 480.039 kb on + strand at 480.060 kb on + strand at 480.063 kb on - strand at 480.075 kb on - strand at 480.108 kb on - strand at 480.196 kb on - strand, within Dsui_0473 at 480.196 kb on - strand at 480.196 kb on - strand at 480.201 kb on - strand, within Dsui_0473 at 480.260 kb on - strand, within Dsui_0473 at 480.260 kb on - strand, within Dsui_0473 at 480.342 kb on + strand, within Dsui_0473 at 480.409 kb on + strand, within Dsui_0473 at 480.468 kb on - strand, within Dsui_0473 at 480.489 kb on + strand, within Dsui_0473 at 480.500 kb on + strand, within Dsui_0473 at 480.556 kb on - strand, within Dsui_0473 at 480.639 kb on - strand at 480.834 kb on + strand, within Dsui_0474 at 480.888 kb on - strand, within Dsui_0474
Per-strain Table
Position Strand Gene LocusTag Fraction incubated with with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate for 3 hours remove 477,995 + Dsui_0470 0.70 +0.9 478,134 + -3.3 478,169 - -1.2 478,169 - +0.9 478,199 + -0.2 478,325 - Dsui_0471 0.22 +0.3 478,341 + +1.0 478,442 - Dsui_0471 0.37 +0.6 478,583 - Dsui_0471 0.56 -0.1 478,720 + -0.9 478,737 + Dsui_0471 0.75 -0.2 478,943 - +0.2 478,943 - +0.2 478,992 - +0.7 479,080 - Dsui_0472 0.13 +0.3 479,138 - Dsui_0472 0.19 +0.4 479,251 + -0.9 479,283 - Dsui_0472 0.33 -2.3 479,335 + +0.7 479,343 - Dsui_0472 0.38 -2.1 479,582 + Dsui_0472 0.61 +0.4 479,678 + -3.3 479,678 + Dsui_0472 0.70 -1.1 479,752 + Dsui_0472 0.77 -2.5 479,891 + -2.4 479,908 + -0.6 479,908 + -1.0 479,908 + -0.6 479,949 + +1.8 479,972 + -1.7 480,039 + -0.1 480,060 + +0.9 480,063 - -1.7 480,075 - +0.8 480,108 - -1.7 480,196 - Dsui_0473 0.21 -0.0 480,196 - -0.3 480,196 - -3.0 480,201 - Dsui_0473 0.21 +0.5 480,260 - Dsui_0473 0.32 -0.6 480,260 - Dsui_0473 0.32 -1.0 480,342 + Dsui_0473 0.46 -0.6 480,409 + Dsui_0473 0.57 +0.2 480,468 - Dsui_0473 0.68 -0.6 480,489 + Dsui_0473 0.71 +1.3 480,500 + Dsui_0473 0.73 +0.1 480,556 - Dsui_0473 0.83 -3.9 480,639 - +0.5 480,834 + Dsui_0474 0.21 -2.5 480,888 - Dsui_0474 0.33 -2.7
Or see this region's nucleotide sequence