Experiment: ICP3_phage_0.41_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_01040 and CSW01_01045 are separated by 160 nucleotides CSW01_01045 and CSW01_01050 overlap by 8 nucleotides CSW01_01050 and CSW01_01055 are separated by 155 nucleotides CSW01_01055 and CSW01_01060 are separated by 35 nucleotides
CSW01_01040: CSW01_01040 - protein RarD, at 200,762 to 201,670
_01040
CSW01_01045: CSW01_01045 - DNA helicase RecQ, at 201,831 to 203,666
_01045
CSW01_01050: CSW01_01050 - DUF3630 domain-containing protein, at 203,659 to 203,958
_01050
CSW01_01055: CSW01_01055 - hypothetical protein, at 204,114 to 204,491
_01055
CSW01_01060: CSW01_01060 - ABC transporter ATP-binding protein, at 204,527 to 206,362
_01060
Position (kb) 201
202
203
204 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3 at 200.836 kb on + strand at 200.879 kb on + strand, within CSW01_01040 at 200.879 kb on + strand, within CSW01_01040 at 200.887 kb on - strand, within CSW01_01040 at 200.900 kb on + strand, within CSW01_01040 at 200.908 kb on - strand, within CSW01_01040 at 200.978 kb on - strand, within CSW01_01040 at 200.980 kb on + strand, within CSW01_01040 at 201.001 kb on - strand, within CSW01_01040 at 201.011 kb on - strand, within CSW01_01040 at 201.036 kb on - strand, within CSW01_01040 at 201.036 kb on - strand, within CSW01_01040 at 201.036 kb on - strand, within CSW01_01040 at 201.036 kb on - strand, within CSW01_01040 at 201.043 kb on + strand, within CSW01_01040 at 201.043 kb on - strand, within CSW01_01040 at 201.051 kb on - strand, within CSW01_01040 at 201.069 kb on + strand, within CSW01_01040 at 201.127 kb on - strand, within CSW01_01040 at 201.127 kb on - strand, within CSW01_01040 at 201.172 kb on + strand, within CSW01_01040 at 201.203 kb on + strand, within CSW01_01040 at 201.203 kb on + strand, within CSW01_01040 at 201.203 kb on + strand, within CSW01_01040 at 201.211 kb on - strand, within CSW01_01040 at 201.218 kb on + strand, within CSW01_01040 at 201.221 kb on - strand, within CSW01_01040 at 201.223 kb on - strand, within CSW01_01040 at 201.258 kb on - strand, within CSW01_01040 at 201.318 kb on + strand, within CSW01_01040 at 201.326 kb on - strand, within CSW01_01040 at 201.331 kb on + strand, within CSW01_01040 at 201.365 kb on + strand, within CSW01_01040 at 201.375 kb on - strand, within CSW01_01040 at 201.375 kb on - strand, within CSW01_01040 at 201.409 kb on - strand, within CSW01_01040 at 201.423 kb on + strand, within CSW01_01040 at 201.451 kb on + strand, within CSW01_01040 at 201.457 kb on + strand, within CSW01_01040 at 201.457 kb on + strand, within CSW01_01040 at 201.460 kb on - strand, within CSW01_01040 at 201.589 kb on + strand at 201.606 kb on + strand at 201.620 kb on - strand at 201.671 kb on + strand at 201.685 kb on - strand at 201.686 kb on - strand at 201.723 kb on + strand at 201.736 kb on - strand at 201.738 kb on - strand at 201.738 kb on - strand at 201.746 kb on - strand at 201.762 kb on - strand at 201.762 kb on - strand at 201.774 kb on - strand at 201.782 kb on + strand at 201.787 kb on - strand at 201.808 kb on - strand at 201.831 kb on - strand at 201.831 kb on - strand at 201.833 kb on - strand at 201.854 kb on + strand at 201.864 kb on - strand at 201.866 kb on + strand at 201.874 kb on - strand at 201.897 kb on + strand at 201.905 kb on - strand at 201.907 kb on - strand at 201.933 kb on + strand at 201.946 kb on - strand at 201.947 kb on + strand at 202.006 kb on + strand at 202.015 kb on + strand, within CSW01_01045 at 202.015 kb on + strand, within CSW01_01045 at 202.015 kb on + strand, within CSW01_01045 at 202.023 kb on - strand, within CSW01_01045 at 202.023 kb on - strand, within CSW01_01045 at 202.023 kb on - strand, within CSW01_01045 at 202.044 kb on - strand, within CSW01_01045 at 202.091 kb on + strand, within CSW01_01045 at 202.091 kb on + strand, within CSW01_01045 at 202.099 kb on - strand, within CSW01_01045 at 202.099 kb on - strand, within CSW01_01045 at 202.099 kb on - strand, within CSW01_01045 at 202.133 kb on - strand, within CSW01_01045 at 202.141 kb on + strand, within CSW01_01045 at 202.184 kb on + strand, within CSW01_01045 at 202.184 kb on + strand, within CSW01_01045 at 202.191 kb on - strand, within CSW01_01045 at 202.192 kb on - strand, within CSW01_01045 at 202.193 kb on + strand, within CSW01_01045 at 202.193 kb on + strand, within CSW01_01045 at 202.193 kb on + strand, within CSW01_01045 at 202.201 kb on - strand, within CSW01_01045 at 202.201 kb on - strand, within CSW01_01045 at 202.246 kb on + strand, within CSW01_01045 at 202.246 kb on + strand, within CSW01_01045 at 202.247 kb on - strand, within CSW01_01045 at 202.249 kb on - strand, within CSW01_01045 at 202.249 kb on - strand, within CSW01_01045 at 202.296 kb on - strand, within CSW01_01045 at 202.330 kb on + strand, within CSW01_01045 at 202.347 kb on - strand, within CSW01_01045 at 202.347 kb on - strand, within CSW01_01045 at 202.347 kb on - strand, within CSW01_01045 at 202.413 kb on + strand, within CSW01_01045 at 202.419 kb on - strand, within CSW01_01045 at 202.430 kb on + strand, within CSW01_01045 at 202.459 kb on + strand, within CSW01_01045 at 202.467 kb on - strand, within CSW01_01045 at 202.467 kb on - strand, within CSW01_01045 at 202.597 kb on + strand, within CSW01_01045 at 202.597 kb on + strand, within CSW01_01045 at 202.605 kb on - strand, within CSW01_01045 at 202.612 kb on - strand, within CSW01_01045 at 202.644 kb on + strand, within CSW01_01045 at 202.663 kb on + strand, within CSW01_01045 at 202.686 kb on - strand, within CSW01_01045 at 202.752 kb on - strand, within CSW01_01045 at 202.786 kb on + strand, within CSW01_01045 at 202.821 kb on + strand, within CSW01_01045 at 202.829 kb on - strand, within CSW01_01045 at 202.831 kb on - strand, within CSW01_01045 at 202.854 kb on - strand, within CSW01_01045 at 202.856 kb on + strand, within CSW01_01045 at 202.864 kb on - strand, within CSW01_01045 at 202.885 kb on + strand, within CSW01_01045 at 202.904 kb on + strand, within CSW01_01045 at 202.912 kb on - strand, within CSW01_01045 at 202.912 kb on - strand, within CSW01_01045 at 203.024 kb on + strand, within CSW01_01045 at 203.057 kb on + strand, within CSW01_01045 at 203.082 kb on - strand, within CSW01_01045 at 203.102 kb on - strand, within CSW01_01045 at 203.145 kb on + strand, within CSW01_01045 at 203.159 kb on - strand, within CSW01_01045 at 203.161 kb on - strand, within CSW01_01045 at 203.209 kb on + strand, within CSW01_01045 at 203.285 kb on - strand, within CSW01_01045 at 203.285 kb on - strand, within CSW01_01045 at 203.308 kb on - strand, within CSW01_01045 at 203.345 kb on - strand, within CSW01_01045 at 203.379 kb on + strand, within CSW01_01045 at 203.459 kb on + strand, within CSW01_01045 at 203.476 kb on - strand, within CSW01_01045 at 203.493 kb on + strand at 203.534 kb on - strand at 203.558 kb on - strand at 203.582 kb on + strand at 203.587 kb on + strand at 203.587 kb on + strand at 203.587 kb on + strand at 203.587 kb on + strand at 203.587 kb on + strand at 203.587 kb on + strand at 203.587 kb on + strand at 203.587 kb on + strand at 203.590 kb on - strand at 203.595 kb on - strand at 203.595 kb on - strand at 203.595 kb on - strand at 203.595 kb on - strand at 203.595 kb on - strand at 203.667 kb on + strand at 203.758 kb on + strand, within CSW01_01050 at 203.804 kb on + strand, within CSW01_01050 at 203.809 kb on + strand, within CSW01_01050 at 203.809 kb on + strand, within CSW01_01050 at 203.809 kb on + strand, within CSW01_01050 at 203.817 kb on - strand, within CSW01_01050 at 203.817 kb on - strand, within CSW01_01050 at 203.817 kb on - strand, within CSW01_01050 at 203.817 kb on - strand, within CSW01_01050 at 203.817 kb on - strand, within CSW01_01050 at 203.817 kb on - strand, within CSW01_01050 at 203.819 kb on - strand, within CSW01_01050 at 203.819 kb on - strand, within CSW01_01050 at 203.894 kb on + strand, within CSW01_01050 at 203.900 kb on + strand, within CSW01_01050 at 203.900 kb on + strand, within CSW01_01050 at 203.905 kb on + strand, within CSW01_01050 at 203.905 kb on + strand, within CSW01_01050 at 203.905 kb on + strand, within CSW01_01050 at 203.905 kb on + strand, within CSW01_01050 at 203.905 kb on + strand, within CSW01_01050 at 203.913 kb on - strand, within CSW01_01050 at 203.913 kb on - strand, within CSW01_01050 at 203.952 kb on + strand at 203.973 kb on + strand at 203.976 kb on - strand at 203.976 kb on - strand at 204.001 kb on - strand at 204.033 kb on + strand at 204.041 kb on + strand at 204.041 kb on - strand at 204.041 kb on - strand at 204.081 kb on + strand at 204.087 kb on - strand at 204.104 kb on + strand at 204.132 kb on + strand at 204.216 kb on + strand, within CSW01_01055 at 204.228 kb on + strand, within CSW01_01055 at 204.233 kb on + strand, within CSW01_01055 at 204.279 kb on + strand, within CSW01_01055 at 204.279 kb on + strand, within CSW01_01055 at 204.279 kb on + strand, within CSW01_01055 at 204.279 kb on + strand, within CSW01_01055 at 204.298 kb on - strand, within CSW01_01055 at 204.316 kb on + strand, within CSW01_01055 at 204.316 kb on + strand, within CSW01_01055 at 204.316 kb on + strand, within CSW01_01055 at 204.316 kb on + strand, within CSW01_01055 at 204.316 kb on + strand, within CSW01_01055 at 204.316 kb on + strand, within CSW01_01055 at 204.316 kb on + strand, within CSW01_01055 at 204.316 kb on + strand, within CSW01_01055 at 204.316 kb on + strand, within CSW01_01055 at 204.316 kb on + strand, within CSW01_01055 at 204.316 kb on + strand, within CSW01_01055 at 204.316 kb on + strand, within CSW01_01055 at 204.324 kb on - strand, within CSW01_01055 at 204.324 kb on - strand, within CSW01_01055 at 204.324 kb on - strand, within CSW01_01055 at 204.324 kb on - strand, within CSW01_01055 at 204.324 kb on - strand, within CSW01_01055 at 204.324 kb on - strand, within CSW01_01055 at 204.324 kb on - strand, within CSW01_01055 at 204.324 kb on - strand, within CSW01_01055 at 204.324 kb on - strand, within CSW01_01055 at 204.324 kb on - strand, within CSW01_01055 at 204.324 kb on - strand, within CSW01_01055 at 204.326 kb on + strand, within CSW01_01055 at 204.326 kb on + strand, within CSW01_01055 at 204.326 kb on + strand, within CSW01_01055 at 204.326 kb on + strand, within CSW01_01055 at 204.334 kb on - strand, within CSW01_01055 at 204.360 kb on + strand, within CSW01_01055 at 204.360 kb on + strand, within CSW01_01055 at 204.368 kb on - strand, within CSW01_01055 at 204.368 kb on - strand, within CSW01_01055 at 204.381 kb on + strand, within CSW01_01055 at 204.447 kb on - strand, within CSW01_01055 at 204.463 kb on - strand at 204.472 kb on + strand at 204.507 kb on + strand at 204.515 kb on - strand at 204.515 kb on - strand at 204.515 kb on - strand at 204.515 kb on - strand at 204.515 kb on - strand at 204.559 kb on - strand at 204.561 kb on + strand at 204.569 kb on - strand at 204.599 kb on + strand at 204.599 kb on + strand at 204.599 kb on + strand at 204.599 kb on + strand at 204.599 kb on + strand at 204.607 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ICP3_phage_0.41_MOI_rep2 remove 200,836 + +1.6 200,879 + CSW01_01040 0.13 +1.3 200,879 + CSW01_01040 0.13 +0.6 200,887 - CSW01_01040 0.14 -1.3 200,900 + CSW01_01040 0.15 +0.8 200,908 - CSW01_01040 0.16 +0.5 200,978 - CSW01_01040 0.24 +0.1 200,980 + CSW01_01040 0.24 +0.7 201,001 - CSW01_01040 0.26 -3.6 201,011 - CSW01_01040 0.27 +1.7 201,036 - CSW01_01040 0.30 -0.9 201,036 - CSW01_01040 0.30 -2.1 201,036 - CSW01_01040 0.30 +0.8 201,036 - CSW01_01040 0.30 -0.4 201,043 + CSW01_01040 0.31 +0.8 201,043 - CSW01_01040 0.31 -0.3 201,051 - CSW01_01040 0.32 -1.7 201,069 + CSW01_01040 0.34 -0.4 201,127 - CSW01_01040 0.40 +1.2 201,127 - CSW01_01040 0.40 +0.3 201,172 + CSW01_01040 0.45 +0.8 201,203 + CSW01_01040 0.49 +1.2 201,203 + CSW01_01040 0.49 +2.2 201,203 + CSW01_01040 0.49 +0.5 201,211 - CSW01_01040 0.49 -3.5 201,218 + CSW01_01040 0.50 -2.9 201,221 - CSW01_01040 0.50 -2.3 201,223 - CSW01_01040 0.51 +0.1 201,258 - CSW01_01040 0.55 -2.4 201,318 + CSW01_01040 0.61 +0.0 201,326 - CSW01_01040 0.62 -1.6 201,331 + CSW01_01040 0.63 +0.1 201,365 + CSW01_01040 0.66 -0.9 201,375 - CSW01_01040 0.67 -2.1 201,375 - CSW01_01040 0.67 -2.7 201,409 - CSW01_01040 0.71 +0.8 201,423 + CSW01_01040 0.73 +0.2 201,451 + CSW01_01040 0.76 -0.4 201,457 + CSW01_01040 0.76 -1.2 201,457 + CSW01_01040 0.76 +1.1 201,460 - CSW01_01040 0.77 +2.1 201,589 + -1.8 201,606 + -3.7 201,620 - +0.5 201,671 + -3.2 201,685 - +0.1 201,686 - -1.1 201,723 + +1.7 201,736 - -3.6 201,738 - +2.1 201,738 - -3.0 201,746 - -3.0 201,762 - +0.5 201,762 - -3.2 201,774 - -2.1 201,782 + -3.5 201,787 - -0.0 201,808 - +0.2 201,831 - -0.6 201,831 - -0.4 201,833 - +2.0 201,854 + -1.3 201,864 - +0.5 201,866 + -0.8 201,874 - -0.2 201,897 + -3.6 201,905 - -4.3 201,907 - -3.6 201,933 + +0.5 201,946 - -0.3 201,947 + +0.3 202,006 + +0.5 202,015 + CSW01_01045 0.10 -0.2 202,015 + CSW01_01045 0.10 +1.1 202,015 + CSW01_01045 0.10 -3.0 202,023 - CSW01_01045 0.10 -3.6 202,023 - CSW01_01045 0.10 -3.6 202,023 - CSW01_01045 0.10 +0.9 202,044 - CSW01_01045 0.12 -1.5 202,091 + CSW01_01045 0.14 -2.0 202,091 + CSW01_01045 0.14 -0.3 202,099 - CSW01_01045 0.15 -1.0 202,099 - CSW01_01045 0.15 -0.9 202,099 - CSW01_01045 0.15 -4.6 202,133 - CSW01_01045 0.16 +0.9 202,141 + CSW01_01045 0.17 -4.4 202,184 + CSW01_01045 0.19 -0.7 202,184 + CSW01_01045 0.19 +0.6 202,191 - CSW01_01045 0.20 -1.5 202,192 - CSW01_01045 0.20 -0.1 202,193 + CSW01_01045 0.20 +2.4 202,193 + CSW01_01045 0.20 -2.0 202,193 + CSW01_01045 0.20 -4.5 202,201 - CSW01_01045 0.20 +0.4 202,201 - CSW01_01045 0.20 +1.7 202,246 + CSW01_01045 0.23 -2.0 202,246 + CSW01_01045 0.23 +0.1 202,247 - CSW01_01045 0.23 +0.9 202,249 - CSW01_01045 0.23 -1.1 202,249 - CSW01_01045 0.23 -3.2 202,296 - CSW01_01045 0.25 -0.4 202,330 + CSW01_01045 0.27 +1.6 202,347 - CSW01_01045 0.28 +0.5 202,347 - CSW01_01045 0.28 -0.4 202,347 - CSW01_01045 0.28 -3.4 202,413 + CSW01_01045 0.32 -4.6 202,419 - CSW01_01045 0.32 +1.9 202,430 + CSW01_01045 0.33 -0.9 202,459 + CSW01_01045 0.34 -0.2 202,467 - CSW01_01045 0.35 +0.2 202,467 - CSW01_01045 0.35 -5.9 202,597 + CSW01_01045 0.42 -2.9 202,597 + CSW01_01045 0.42 -0.9 202,605 - CSW01_01045 0.42 +2.3 202,612 - CSW01_01045 0.43 -3.1 202,644 + CSW01_01045 0.44 +2.4 202,663 + CSW01_01045 0.45 +0.1 202,686 - CSW01_01045 0.47 -0.1 202,752 - CSW01_01045 0.50 -0.1 202,786 + CSW01_01045 0.52 -4.4 202,821 + CSW01_01045 0.54 -4.0 202,829 - CSW01_01045 0.54 -0.1 202,831 - CSW01_01045 0.54 -0.2 202,854 - CSW01_01045 0.56 +1.4 202,856 + CSW01_01045 0.56 -0.1 202,864 - CSW01_01045 0.56 -2.9 202,885 + CSW01_01045 0.57 +0.9 202,904 + CSW01_01045 0.58 -0.7 202,912 - CSW01_01045 0.59 -0.2 202,912 - CSW01_01045 0.59 -0.1 203,024 + CSW01_01045 0.65 -1.4 203,057 + CSW01_01045 0.67 +0.8 203,082 - CSW01_01045 0.68 +1.2 203,102 - CSW01_01045 0.69 -1.3 203,145 + CSW01_01045 0.72 -0.0 203,159 - CSW01_01045 0.72 -0.5 203,161 - CSW01_01045 0.72 +0.5 203,209 + CSW01_01045 0.75 -1.1 203,285 - CSW01_01045 0.79 -0.1 203,285 - CSW01_01045 0.79 -3.3 203,308 - CSW01_01045 0.80 +0.7 203,345 - CSW01_01045 0.82 -2.9 203,379 + CSW01_01045 0.84 +1.3 203,459 + CSW01_01045 0.89 +0.1 203,476 - CSW01_01045 0.90 -0.5 203,493 + -0.2 203,534 - +0.4 203,558 - -2.3 203,582 + -1.0 203,587 + -2.3 203,587 + -1.6 203,587 + +0.5 203,587 + +0.8 203,587 + -0.3 203,587 + -3.6 203,587 + -2.4 203,587 + -0.1 203,590 - -0.9 203,595 - +0.5 203,595 - -2.3 203,595 - +1.4 203,595 - +1.4 203,595 - -0.3 203,667 + +0.0 203,758 + CSW01_01050 0.33 +1.3 203,804 + CSW01_01050 0.48 -0.2 203,809 + CSW01_01050 0.50 +0.2 203,809 + CSW01_01050 0.50 -0.8 203,809 + CSW01_01050 0.50 +0.9 203,817 - CSW01_01050 0.53 +1.1 203,817 - CSW01_01050 0.53 -4.0 203,817 - CSW01_01050 0.53 -0.0 203,817 - CSW01_01050 0.53 -3.6 203,817 - CSW01_01050 0.53 -0.3 203,817 - CSW01_01050 0.53 -4.2 203,819 - CSW01_01050 0.53 +0.7 203,819 - CSW01_01050 0.53 +0.5 203,894 + CSW01_01050 0.78 -3.7 203,900 + CSW01_01050 0.80 +1.4 203,900 + CSW01_01050 0.80 +2.1 203,905 + CSW01_01050 0.82 -1.5 203,905 + CSW01_01050 0.82 +0.4 203,905 + CSW01_01050 0.82 +1.4 203,905 + CSW01_01050 0.82 +0.3 203,905 + CSW01_01050 0.82 -0.9 203,913 - CSW01_01050 0.85 +2.1 203,913 - CSW01_01050 0.85 -3.5 203,952 + -0.7 203,973 + +2.5 203,976 - +1.1 203,976 - +0.9 204,001 - -1.1 204,033 + -0.2 204,041 + +0.2 204,041 - +1.5 204,041 - -0.5 204,081 + -1.7 204,087 - -3.6 204,104 + +0.1 204,132 + +1.1 204,216 + CSW01_01055 0.27 +1.8 204,228 + CSW01_01055 0.30 +0.3 204,233 + CSW01_01055 0.31 +0.4 204,279 + CSW01_01055 0.44 +1.0 204,279 + CSW01_01055 0.44 -1.8 204,279 + CSW01_01055 0.44 +2.4 204,279 + CSW01_01055 0.44 -2.8 204,298 - CSW01_01055 0.49 -4.1 204,316 + CSW01_01055 0.53 -0.1 204,316 + CSW01_01055 0.53 -0.9 204,316 + CSW01_01055 0.53 -1.2 204,316 + CSW01_01055 0.53 +1.2 204,316 + CSW01_01055 0.53 -2.6 204,316 + CSW01_01055 0.53 -1.6 204,316 + CSW01_01055 0.53 +1.2 204,316 + CSW01_01055 0.53 -1.1 204,316 + CSW01_01055 0.53 +0.1 204,316 + CSW01_01055 0.53 +2.1 204,316 + CSW01_01055 0.53 +0.5 204,316 + CSW01_01055 0.53 -4.3 204,324 - CSW01_01055 0.56 -0.1 204,324 - CSW01_01055 0.56 -1.2 204,324 - CSW01_01055 0.56 -4.5 204,324 - CSW01_01055 0.56 -0.3 204,324 - CSW01_01055 0.56 -1.9 204,324 - CSW01_01055 0.56 +0.3 204,324 - CSW01_01055 0.56 -1.7 204,324 - CSW01_01055 0.56 +2.1 204,324 - CSW01_01055 0.56 +0.1 204,324 - CSW01_01055 0.56 +3.2 204,324 - CSW01_01055 0.56 +0.3 204,326 + CSW01_01055 0.56 +0.2 204,326 + CSW01_01055 0.56 +0.5 204,326 + CSW01_01055 0.56 +1.1 204,326 + CSW01_01055 0.56 +0.8 204,334 - CSW01_01055 0.58 -0.9 204,360 + CSW01_01055 0.65 +1.0 204,360 + CSW01_01055 0.65 -0.9 204,368 - CSW01_01055 0.67 +0.9 204,368 - CSW01_01055 0.67 -2.7 204,381 + CSW01_01055 0.71 +1.3 204,447 - CSW01_01055 0.88 -0.9 204,463 - -1.8 204,472 + +0.9 204,507 + -0.3 204,515 - -0.3 204,515 - +0.9 204,515 - +2.9 204,515 - -3.1 204,515 - -3.8 204,559 - -2.3 204,561 + -5.1 204,569 - +0.9 204,599 + +1.5 204,599 + -0.5 204,599 + -1.0 204,599 + +0.2 204,599 + +0.5 204,607 - +1.0
Or see this region's nucleotide sequence