Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01720

Experiment: L-Sorbose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01710 and BWI76_RS01715 are separated by 31 nucleotidesBWI76_RS01715 and BWI76_RS01720 overlap by 8 nucleotidesBWI76_RS01720 and BWI76_RS01725 are separated by 123 nucleotidesBWI76_RS01725 and BWI76_RS01730 are separated by 11 nucleotides BWI76_RS01710: BWI76_RS01710 - DNA gyrase inhibitor, at 351,356 to 351,673 _RS01710 BWI76_RS01715: BWI76_RS01715 - shikimate 5-dehydrogenase, at 351,705 to 352,514 _RS01715 BWI76_RS01720: BWI76_RS01720 - L-sorbose 1-phosphate reductase (EC 1.1.1.-) (from data), at 352,507 to 353,736 _RS01720 BWI76_RS01725: BWI76_RS01725 - PTS mannose/fructose/sorbose family transporter subunit IID, at 353,860 to 354,684 _RS01725 BWI76_RS01730: BWI76_RS01730 - PTS mannose/fructose/sorbose transporter subunit IIC, at 354,696 to 355,493 _RS01730 Position (kb) 352 353 354Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 351.622 kb on + strand, within BWI76_RS01710at 351.622 kb on + strand, within BWI76_RS01710at 351.627 kb on + strand, within BWI76_RS01710at 351.627 kb on + strand, within BWI76_RS01710at 351.627 kb on + strand, within BWI76_RS01710at 351.630 kb on - strand, within BWI76_RS01710at 351.630 kb on - strand, within BWI76_RS01710at 351.630 kb on - strand, within BWI76_RS01710at 351.635 kb on - strand, within BWI76_RS01710at 351.682 kb on + strandat 351.682 kb on + strandat 351.690 kb on - strandat 351.722 kb on - strandat 351.777 kb on + strandat 351.834 kb on + strand, within BWI76_RS01715at 351.988 kb on - strand, within BWI76_RS01715at 351.999 kb on - strand, within BWI76_RS01715at 352.145 kb on - strand, within BWI76_RS01715at 352.218 kb on - strand, within BWI76_RS01715at 352.249 kb on + strand, within BWI76_RS01715at 352.280 kb on + strand, within BWI76_RS01715at 352.309 kb on - strand, within BWI76_RS01715at 352.346 kb on - strand, within BWI76_RS01715at 352.391 kb on - strand, within BWI76_RS01715at 352.392 kb on + strand, within BWI76_RS01715at 352.416 kb on - strand, within BWI76_RS01715at 352.486 kb on + strandat 352.687 kb on + strand, within BWI76_RS01720at 352.722 kb on + strand, within BWI76_RS01720at 352.724 kb on - strand, within BWI76_RS01720at 352.930 kb on + strand, within BWI76_RS01720at 353.173 kb on + strand, within BWI76_RS01720at 353.189 kb on + strand, within BWI76_RS01720at 353.450 kb on - strand, within BWI76_RS01720at 353.467 kb on + strand, within BWI76_RS01720at 353.467 kb on + strand, within BWI76_RS01720at 353.721 kb on + strandat 353.748 kb on + strandat 353.815 kb on + strandat 353.942 kb on - strandat 353.951 kb on + strand, within BWI76_RS01725at 353.978 kb on - strand, within BWI76_RS01725at 353.985 kb on + strand, within BWI76_RS01725at 353.990 kb on + strand, within BWI76_RS01725at 354.077 kb on - strand, within BWI76_RS01725at 354.134 kb on - strand, within BWI76_RS01725at 354.182 kb on + strand, within BWI76_RS01725at 354.223 kb on + strand, within BWI76_RS01725at 354.335 kb on + strand, within BWI76_RS01725at 354.477 kb on + strand, within BWI76_RS01725at 354.718 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Sorbose (C)
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351,622 + BWI76_RS01710 0.84 -0.0
351,622 + BWI76_RS01710 0.84 +0.3
351,627 + BWI76_RS01710 0.85 -0.2
351,627 + BWI76_RS01710 0.85 -1.8
351,627 + BWI76_RS01710 0.85 +0.8
351,630 - BWI76_RS01710 0.86 +0.3
351,630 - BWI76_RS01710 0.86 +2.8
351,630 - BWI76_RS01710 0.86 +0.1
351,635 - BWI76_RS01710 0.88 +0.5
351,682 + -0.1
351,682 + -0.3
351,690 - -1.0
351,722 - +0.7
351,777 + -1.3
351,834 + BWI76_RS01715 0.16 -0.4
351,988 - BWI76_RS01715 0.35 -0.3
351,999 - BWI76_RS01715 0.36 -3.2
352,145 - BWI76_RS01715 0.54 -0.5
352,218 - BWI76_RS01715 0.63 -1.4
352,249 + BWI76_RS01715 0.67 -1.6
352,280 + BWI76_RS01715 0.71 +1.5
352,309 - BWI76_RS01715 0.75 -1.8
352,346 - BWI76_RS01715 0.79 -1.8
352,391 - BWI76_RS01715 0.85 -0.7
352,392 + BWI76_RS01715 0.85 -2.4
352,416 - BWI76_RS01715 0.88 -1.8
352,486 + -1.6
352,687 + BWI76_RS01720 0.15 -4.1
352,722 + BWI76_RS01720 0.17 -2.4
352,724 - BWI76_RS01720 0.18 -0.4
352,930 + BWI76_RS01720 0.34 -2.4
353,173 + BWI76_RS01720 0.54 -5.2
353,189 + BWI76_RS01720 0.55 -3.8
353,450 - BWI76_RS01720 0.77 -1.7
353,467 + BWI76_RS01720 0.78 -3.2
353,467 + BWI76_RS01720 0.78 -1.9
353,721 + -4.6
353,748 + -1.1
353,815 + -3.9
353,942 - +0.0
353,951 + BWI76_RS01725 0.11 -2.7
353,978 - BWI76_RS01725 0.14 -1.1
353,985 + BWI76_RS01725 0.15 -2.8
353,990 + BWI76_RS01725 0.16 +0.1
354,077 - BWI76_RS01725 0.26 -3.0
354,134 - BWI76_RS01725 0.33 -2.7
354,182 + BWI76_RS01725 0.39 -2.4
354,223 + BWI76_RS01725 0.44 +0.2
354,335 + BWI76_RS01725 0.58 -4.4
354,477 + BWI76_RS01725 0.75 -1.7
354,718 - -3.6

Or see this region's nucleotide sequence