Experiment: Recovery broth with Nitrofurantoin 0.03 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14490 and BBR_RS14495 are separated by 54 nucleotides BBR_RS14495 and BBR_RS14500 are separated by 183 nucleotides
BBR_RS14490: BBR_RS14490 - ribonuclease D, at 1,025,170 to 1,026,471
_RS14490
BBR_RS14495: BBR_RS14495 - trigger factor, at 1,026,526 to 1,027,899
_RS14495
BBR_RS14500: BBR_RS14500 - hypothetical protein, at 1,028,083 to 1,029,483
_RS14500
Position (kb)
1026
1027
1028 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1025.667 kb on - strand, within BBR_RS14490 at 1025.681 kb on - strand, within BBR_RS14490 at 1025.683 kb on - strand, within BBR_RS14490 at 1025.698 kb on - strand, within BBR_RS14490 at 1025.726 kb on + strand, within BBR_RS14490 at 1025.739 kb on - strand, within BBR_RS14490 at 1025.777 kb on + strand, within BBR_RS14490 at 1025.785 kb on - strand, within BBR_RS14490 at 1025.787 kb on + strand, within BBR_RS14490 at 1025.787 kb on + strand, within BBR_RS14490 at 1025.851 kb on + strand, within BBR_RS14490 at 1025.883 kb on + strand, within BBR_RS14490 at 1025.915 kb on - strand, within BBR_RS14490 at 1025.944 kb on - strand, within BBR_RS14490 at 1025.949 kb on - strand, within BBR_RS14490 at 1025.952 kb on - strand, within BBR_RS14490 at 1025.960 kb on - strand, within BBR_RS14490 at 1025.960 kb on - strand, within BBR_RS14490 at 1026.021 kb on - strand, within BBR_RS14490 at 1026.031 kb on - strand, within BBR_RS14490 at 1026.035 kb on - strand, within BBR_RS14490 at 1026.037 kb on - strand, within BBR_RS14490 at 1026.056 kb on - strand, within BBR_RS14490 at 1026.133 kb on + strand, within BBR_RS14490 at 1026.133 kb on + strand, within BBR_RS14490 at 1026.146 kb on - strand, within BBR_RS14490 at 1026.155 kb on + strand, within BBR_RS14490 at 1026.212 kb on - strand, within BBR_RS14490 at 1026.261 kb on - strand, within BBR_RS14490 at 1026.265 kb on + strand, within BBR_RS14490 at 1026.279 kb on - strand, within BBR_RS14490 at 1026.517 kb on + strand at 1026.639 kb on + strand at 1026.760 kb on + strand, within BBR_RS14495 at 1027.204 kb on + strand, within BBR_RS14495 at 1027.217 kb on + strand, within BBR_RS14495 at 1027.257 kb on + strand, within BBR_RS14495 at 1027.304 kb on + strand, within BBR_RS14495 at 1027.425 kb on + strand, within BBR_RS14495 at 1027.461 kb on + strand, within BBR_RS14495 at 1027.689 kb on + strand, within BBR_RS14495 at 1027.719 kb on + strand, within BBR_RS14495 at 1027.855 kb on + strand at 1027.882 kb on + strand at 1028.003 kb on + strand at 1028.003 kb on + strand at 1028.003 kb on + strand at 1028.024 kb on - strand at 1028.041 kb on + strand at 1028.069 kb on + strand at 1028.069 kb on + strand at 1028.085 kb on - strand at 1028.085 kb on - strand at 1028.132 kb on + strand at 1028.146 kb on - strand at 1028.184 kb on + strand at 1028.184 kb on + strand at 1028.192 kb on - strand at 1028.226 kb on - strand, within BBR_RS14500 at 1028.243 kb on + strand, within BBR_RS14500 at 1028.243 kb on - strand, within BBR_RS14500 at 1028.432 kb on - strand, within BBR_RS14500 at 1028.464 kb on + strand, within BBR_RS14500 at 1028.528 kb on + strand, within BBR_RS14500 at 1028.552 kb on - strand, within BBR_RS14500 at 1028.642 kb on + strand, within BBR_RS14500 at 1028.643 kb on - strand, within BBR_RS14500 at 1028.671 kb on - strand, within BBR_RS14500 at 1028.711 kb on - strand, within BBR_RS14500 at 1028.730 kb on - strand, within BBR_RS14500 at 1028.756 kb on + strand, within BBR_RS14500 at 1028.756 kb on + strand, within BBR_RS14500 at 1028.779 kb on + strand, within BBR_RS14500 at 1028.782 kb on - strand, within BBR_RS14500 at 1028.784 kb on + strand, within BBR_RS14500 at 1028.792 kb on - strand, within BBR_RS14500 at 1028.792 kb on - strand, within BBR_RS14500 at 1028.792 kb on - strand, within BBR_RS14500 at 1028.827 kb on + strand, within BBR_RS14500 at 1028.835 kb on - strand, within BBR_RS14500 at 1028.839 kb on + strand, within BBR_RS14500 at 1028.893 kb on + strand, within BBR_RS14500
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Nitrofurantoin 0.03 mM remove 1,025,667 - BBR_RS14490 0.38 -1.0 1,025,681 - BBR_RS14490 0.39 -1.0 1,025,683 - BBR_RS14490 0.39 -0.6 1,025,698 - BBR_RS14490 0.41 -0.9 1,025,726 + BBR_RS14490 0.43 -2.0 1,025,739 - BBR_RS14490 0.44 -0.0 1,025,777 + BBR_RS14490 0.47 +0.4 1,025,785 - BBR_RS14490 0.47 -0.1 1,025,787 + BBR_RS14490 0.47 -1.8 1,025,787 + BBR_RS14490 0.47 +0.0 1,025,851 + BBR_RS14490 0.52 -0.2 1,025,883 + BBR_RS14490 0.55 -0.7 1,025,915 - BBR_RS14490 0.57 -0.3 1,025,944 - BBR_RS14490 0.59 +1.1 1,025,949 - BBR_RS14490 0.60 -1.2 1,025,952 - BBR_RS14490 0.60 -1.1 1,025,960 - BBR_RS14490 0.61 +0.6 1,025,960 - BBR_RS14490 0.61 -1.4 1,026,021 - BBR_RS14490 0.65 -1.0 1,026,031 - BBR_RS14490 0.66 -1.7 1,026,035 - BBR_RS14490 0.66 -0.2 1,026,037 - BBR_RS14490 0.67 +0.7 1,026,056 - BBR_RS14490 0.68 -0.9 1,026,133 + BBR_RS14490 0.74 -1.4 1,026,133 + BBR_RS14490 0.74 -0.6 1,026,146 - BBR_RS14490 0.75 -0.9 1,026,155 + BBR_RS14490 0.76 -0.3 1,026,212 - BBR_RS14490 0.80 +2.6 1,026,261 - BBR_RS14490 0.84 -0.6 1,026,265 + BBR_RS14490 0.84 -1.2 1,026,279 - BBR_RS14490 0.85 -0.1 1,026,517 + +0.3 1,026,639 + -0.3 1,026,760 + BBR_RS14495 0.17 -0.0 1,027,204 + BBR_RS14495 0.49 -2.0 1,027,217 + BBR_RS14495 0.50 +0.9 1,027,257 + BBR_RS14495 0.53 +1.9 1,027,304 + BBR_RS14495 0.57 -1.8 1,027,425 + BBR_RS14495 0.65 -0.7 1,027,461 + BBR_RS14495 0.68 -0.5 1,027,689 + BBR_RS14495 0.85 +0.7 1,027,719 + BBR_RS14495 0.87 -2.6 1,027,855 + -2.5 1,027,882 + +2.9 1,028,003 + -1.3 1,028,003 + +0.3 1,028,003 + -0.1 1,028,024 - +0.3 1,028,041 + -1.8 1,028,069 + +0.8 1,028,069 + +0.5 1,028,085 - -0.5 1,028,085 - -0.5 1,028,132 + +0.8 1,028,146 - -0.9 1,028,184 + +0.6 1,028,184 + +0.3 1,028,192 - +0.5 1,028,226 - BBR_RS14500 0.10 -1.8 1,028,243 + BBR_RS14500 0.11 -0.7 1,028,243 - BBR_RS14500 0.11 -1.5 1,028,432 - BBR_RS14500 0.25 -0.8 1,028,464 + BBR_RS14500 0.27 +0.5 1,028,528 + BBR_RS14500 0.32 -0.0 1,028,552 - BBR_RS14500 0.33 +0.5 1,028,642 + BBR_RS14500 0.40 +0.6 1,028,643 - BBR_RS14500 0.40 +0.6 1,028,671 - BBR_RS14500 0.42 +0.1 1,028,711 - BBR_RS14500 0.45 +0.4 1,028,730 - BBR_RS14500 0.46 -0.6 1,028,756 + BBR_RS14500 0.48 +0.1 1,028,756 + BBR_RS14500 0.48 -0.6 1,028,779 + BBR_RS14500 0.50 +0.4 1,028,782 - BBR_RS14500 0.50 -1.4 1,028,784 + BBR_RS14500 0.50 -0.3 1,028,792 - BBR_RS14500 0.51 -0.9 1,028,792 - BBR_RS14500 0.51 -0.5 1,028,792 - BBR_RS14500 0.51 +0.4 1,028,827 + BBR_RS14500 0.53 -0.9 1,028,835 - BBR_RS14500 0.54 +0.3 1,028,839 + BBR_RS14500 0.54 -1.6 1,028,893 + BBR_RS14500 0.58 -0.7
Or see this region's nucleotide sequence